LOCUS       NZ_CP056706             3258 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00577 plasmid pRHBSTW-00577_6,
            complete sequence.
ACCESSION   NZ_CP056706
VERSION     NZ_CP056706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148657
            Assembly: GCF_013735435.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00577
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.2905475056742x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013735435.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:29:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,946
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,678
            CDSs (with protein)               :: 5,678
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 53 of 151
            Pseudo Genes (incomplete)         :: 102 of 151
            Pseudo Genes (internal stop)      :: 20 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00577"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00577_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            267..458
                     /locus_tag="HV240_RS29525"
                     /old_locus_tag="HV240_29530"
     CDS             267..458
                     /locus_tag="HV240_RS29525"
                     /old_locus_tag="HV240_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            458..880
                     /locus_tag="HV240_RS29530"
                     /old_locus_tag="HV240_29535"
     CDS             458..880
                     /locus_tag="HV240_RS29530"
                     /old_locus_tag="HV240_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1226..1435)
                     /locus_tag="HV240_RS29535"
                     /old_locus_tag="HV240_29540"
     CDS             complement(1226..1435)
                     /locus_tag="HV240_RS29535"
                     /old_locus_tag="HV240_29540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158239539.1"
                     /translation="MNALKATTNHPDNGERMRNLLRGREALPAQPENRFSARSGIGFVD
                     GRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            2089..2763
                     /locus_tag="HV240_RS29540"
                     /old_locus_tag="HV240_29545"
     CDS             2089..2763
                     /locus_tag="HV240_RS29540"
                     /old_locus_tag="HV240_29545"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_065806389.1"
                     /translation="MIPNWAPMFSWWVHNGLIRKFNWRNDEIGKPSAKVAALMIYIAIT
                     MKCAEEVGIQEVKITYTRLSEMTGISRQLIREGTAILVKLDMIDVTRIGRNNTYKLISN
                     KERSGWCKIPVRAITSEGGHAITPFEDLKLRRKVELHALKIFIYLASVRDNYTEYSLAS
                     YQTISSAVGITEKDIQRALALLSLVGLITRISSEKADDTKKNQPNRYYLAGYKSFVKRS
                     DW"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ggaaacgccc ggtatcttta agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
       61 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggccgctgt gctgccttct
      121 ccctctccga gctgtctgct ctatgtcgtt atgtgcgcgt tgcgaccgct cgccgcagcc
      181 gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagatc tgtctgacat
      241 tgagcactga cgctctgagg ccctttatga cgaaacaaca gaaaacagcg ctgaacatgg
      301 ctaaattcat ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatctcg
      361 atgttgaggc cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac
      421 tgtcctcgag actggatgaa ttacaggatg gtaattgatg gccagagtgt ctattagtga
      481 agcggcacgg cttgttaagg ttagccgtcc cacaatttac aagatgatta acagtgggaa
      541 gttaagttac acctctgttg taaagcacgg gaaggctatt aaagttattg atacttcaga
      601 gcttattaga gtgtttggtt ctcttgatgg tgtcatcgat actgtaaaat atgatgtaaa
      661 aagtgacgca gaatctacaa gtattaacag cgttggttta catgatttac aacatagaat
      721 cgctctactt gaagctgaaa acgatggtct taaaggggct gttaaggctc gagatgagca
      781 tattgattcc cttcgtcagg ctatgcagct tctggagcac aagcgcgagc cttcttcacc
      841 accccattcc ccgtggtgga aattctggaa aaaatcctga tcctgccatg cttgagtttt
      901 cctctccggc catcccaggg agttcctgaa ccttcccaaa aatcgccaga tctctgaacg
      961 ctcctcacga ctaaaaatac ccacacacta cgcaaaatta aaattttgca gattccttat
     1021 ttaaaacaat tggttaatga aaaattaatg tgcagtgatg tgttacaaag tgcagatcca
     1081 agtcgtggac gactctatgg cgaatcgttt acgaattgcc cacgattcat atgtgaatca
     1141 cgctttatta tcatgttgtt atgatgaatc gactcttgat tcaattcatc aatttgggcg
     1201 ttttttgttc atggtgactc ttgattcaat tcacgattcg aatcgccggt gaatcgtgtg
     1261 gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtcgacgaac ccgattcctg
     1321 aacgtgctga aaaccggttt tccggctgtg ccggcaacgc ctcacggcca cgcaataaat
     1381 tgcgcatccg ttcaccgtta tcggggtggt tcgtggtggc tttcagggcg ttcatcggat
     1441 tttagccgct aaaatcccgg ccaggtatgt ccgtgcatag ccatgcagtc gcgccagaat
     1501 catcaggggc gttttttctc gatatgggca cagaaaggtg tgtgtttcag gccggaggcc
     1561 tcagaaacgc cctgagggcc tctgaggcgg tgcgggcaac ggatgttatg gtaaatagct
     1621 cgactctgtg gctcttactc gacgataaaa aaatagtatt ataaaacaac accttacaaa
     1681 aaacatatat ttatcaatgg tttatgtttg ttttttgtga actaatagtt tatcaatttg
     1741 ggcgtttttt gactgataaa tggtctttta tcgcgctaaa tttcatctta tgaactaatt
     1801 tttagatgct aaaaactaat gattctaaaa gtaagtgatt gattaaattg gttttatttt
     1861 ttattttcaa aaaaaattaa aaccattgtc tctagatgta aatcctttgg cgagataaaa
     1921 tttgaaaata actcctcgta gaaggacctt ttatggaaaa tttaaccgtt atcagttcat
     1981 ttattacctc aatagcccct gtcatcagtg ggttcgttcc gttcctgttg tcattagctg
     2041 ttttggcagg cttcgttgct atttgcatta ttggctggag gaagtgagat gatccctaat
     2101 tgggctccga tgttctcttg gtgggttcat aacggactta ttcggaaatt taattggcgt
     2161 aacgatgaga tcggcaaacc atcagcaaag gttgccgctc ttatgattta catagcaatt
     2221 accatgaaat gcgctgaaga agttggcatt caggaagtga aaataaccta tacccggcta
     2281 tcagaaatga cagggattag tcggcagctg ataagagaag gtacagctat tttagttaag
     2341 ctggatatga ttgatgttac gcggattggt cgaaacaaca cctataaact gataagcaat
     2401 aaagagcgta gtggatggtg caagatacct gtaagagcaa taactagtga aggtggccat
     2461 gctattacac catttgaaga tttaaagcta aggcgaaaag tagagctgca tgcgctaaaa
     2521 atattcattt atcttgcttc tgttcgtgat aactacacag agtattcgtt agcttcttac
     2581 cagactataa gtagtgctgt tggcattaca gaaaaagata tccagagagc attagcactc
     2641 ctctcattgg taggtctgat cactaggatt tctagtgaaa aagctgatga cactaaaaag
     2701 aatcagccta atcgctacta tcttgctgga tataaaagct ttgttaaaag atcggactgg
     2761 tgaatacgag gtgtgcatag gcagggatgg gtttaacgct aagctaggaa tgcattcttg
     2821 acgttactct ttggctaaga gtatcgttac gcattaacga aaaaaccgcc ctgcaaggcg
     2881 gtttttcgaa ggttaagtca gttggggaac tgcttaaccg tggtaacagg gtgaacaaga
     2941 ccgctgccgc caaatttctc tttaagttta gccgttgtta ggctcaaaca tcaagactaa
     3001 agaatctata ggcatcttgt taccccgtta ccagtggcta ccgccaatgg cgatttgtcg
     3061 tgtctgctcc gggttggact caagtgatag ttaccggaaa gggcgtggta gtcgggctga
     3121 acggggggtt cttgcataca gcccagcttg gagcgaactg tctaaacgga gcgggacgtg
     3181 gtgatttagg taaggcttcc accacaacac gtaaaccgca gggcgggaac aggaaagcgc
     3241 aagagggagc caccgggg
//
