LOCUS       NZ_CP056705             4843 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00577 plasmid pRHBSTW-00577_5,
            complete sequence.
ACCESSION   NZ_CP056705
VERSION     NZ_CP056705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148657
            Assembly: GCF_013735435.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00577
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.2905475056742x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013735435.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:29:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,946
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,678
            CDSs (with protein)               :: 5,678
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 53 of 151
            Pseudo Genes (incomplete)         :: 102 of 151
            Pseudo Genes (internal stop)      :: 20 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00577"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00577_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(<335..988)
                     /locus_tag="HV240_RS29915"
                     /old_locus_tag="HV240_29510"
                     /pseudo=""
     CDS             complement(<335..988)
                     /locus_tag="HV240_RS29915"
                     /old_locus_tag="HV240_29510"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(978..1301)
                     /locus_tag="HV240_RS29510"
                     /old_locus_tag="HV240_29515"
     CDS             complement(978..1301)
                     /locus_tag="HV240_RS29510"
                     /old_locus_tag="HV240_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2360..4168)
                     /locus_tag="HV240_RS29515"
                     /old_locus_tag="HV240_29520"
     CDS             complement(2360..4168)
                     /locus_tag="HV240_RS29515"
                     /old_locus_tag="HV240_29520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_227627439.1"
                     /translation="MAGEAFAGFRGLHAVFGLNPAVIHLAAWLCSWVKFICRDRGGLPD
                     GLLLVLADCRPVRGEAVRGGAGKGCYGKCGIFYYMECIFMRIKKPHLRPIVITLSVGGI
                     LLTSIFLIAVIMVFQRENIEKDLLYTNSSYAMKMSDVMSGYIEMAQGELAYGAKKIKST
                     NDIDSLKDEADRLRLQSGMFNSVFVVSNDAVVLATSPESLDLVGTRLNSSASKLAIEEK
                     KPFISSPFKSVTGNLIILLLHPIYDKGGNYIGYVGGSIYLKKHSLFSDVLSRHFFKNNT
                     EISIVSDDGNVIFNNDKSAVDRPMVMSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMK
                     NTAWNVLVYSRADSVTTILIAYAKSVLVLLIIIIIVFSLISYFIASSISLPLEKLAIST
                     NAKDIGASLVYIKAINAWYAEADRLKKALFTHVKVMMERVDSLNEVALKDPLTGVNNRL
                     GFSTKIQNNKYGAGASVIAIDVDFFKEINDSFGHGVGDEVLVSLAQIIKSCCRSEDIIC
                     RFGGEEFVVFLPNTSVVTAECVAERIRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDF
                     LLKNADDALYQAKREGRNKVIVYSAI"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(4430..4645)
                     /locus_tag="HV240_RS29520"
                     /old_locus_tag="HV240_29525"
     CDS             complement(4430..4645)
                     /locus_tag="HV240_RS29520"
                     /old_locus_tag="HV240_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137500696.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSYLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1415..1587)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.701"
                     /coverage="100.0"
                     /evalue="4.2800000000000005e-79"
     Typing          1512..2064
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(3..988)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.441"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(985..1301)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.65e-61"
     Typing          complement(4339..4841)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.024"
                     /coverage="99.0"
                     /evalue="6.92e-97"
ORIGIN
        1 cccctgccac cacagtatgc tcccgctcgt gccgatcagc agcgctgaca ccatcaggat
       61 ggtgagccat gtccgcccgg cagtccgcag tacgctccgc cggtttttct ccatcgcctc
      121 gctcatactc tgctcgtgtg cgaggatggc gtcgctgatt ctcctcgcgc ttgcgctcag
      181 ttccgctctg acagactgct cgtgctcgct gaatgcggct ttcagcatct cgccggtact
      241 ctgcttctgc gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgctcat
      301 agatggctcc cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatactgc
      361 ttttcgtggt gcgcaccacc tcaaaacctg accgttccag ggcctcagtg acgtcctgac
      421 ggtttttcag ctccccggac gaagcaaggg tcagcagtcc gcgcgtgatg gcttctgcgg
      481 cctcctgctt cgttttcggc agtccggacg gcgtgaccag cgcccggcgg ttttccggtg
      541 cgttcgggtc gtgcagcccc agtctgccgt tcacgatggt ctgccaggca tcgatacgcg
      601 gccggtcggc gcggtcgtag tacggctgga gccgcctgcc ggtcagcagc tcggtattgg
      661 ggatcagaaa gttcagctcc agacgcccct tgtccgcgtg ctccacccac agaatgctgt
      721 actgatcttt atcaagtccg ggcatcagaa cgcgctcgaa gctggccatc agtttttccc
      781 gctggccggg aggcagatcg gcctccgcaa acgacaggac gccggatgtg tatttcttca
      841 cgtaaggcga cgcgtcaatc agctcccgga cttcttccgg cttcccctgc aggacgctgg
      901 caccttcacg ctgccggtct ttacccagca gatagtccac cggcccggcg ccaccgccgc
      961 gaccgcgtgg atgaaattta acgatcatcg tccgttcctt tctcaagaac ggcgtgcctc
     1021 agccgctcaa gcccggcatc gatggccatc agcgccgcca caatctgtac cctgtcatgg
     1081 ccggtgccgc cgccggcgtt aacacggcgg gctatctggt tcaggttatt gcccatgccg
     1141 gcaagctggc gcagcagcgc cggcgagatt gaggggagct ttccggaacg tgccggcttt
     1201 tcgtccaggc aggtctgccg catccacgcc gccagctgcc tgccgtcaca gcgctcaagc
     1261 agccgccggt gctcgtcctc cgtcacccac atggtgagca tcttgttccg tttctctgac
     1321 aaaccggcct cctgataacg ccgggacggg cgggaaagcc tttcccggtc ggcgcggggt
     1381 tggacaagcc gcaggcgcgt caggcttttg gcgggtttcg gggcgcagcc ctgaaccagt
     1441 cacgcagcgc tagcggagtg tatactggct tactatgttg gcacggatgc gagtgtcagt
     1501 gaagtgcttc atgtggcagg agaaaaatgc agcagcggag cgtcagcaga atatgtgata
     1561 caggatatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1621 agcggtaccg gcttacaggc ggggcggaaa tttcctggga gatgccagga agaagcttaa
     1681 cagggaagcg ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
     1741 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     1801 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1861 gatgtcaatc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcggttccgg
     1921 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagc ccgactacca
     1981 cgttgtcccg gtaactatca tcttgagtcc aacccggaac agacacgaaa aatcgccagt
     2041 ggcggtagcc attagtaaga ggattcacgg agcggagtag tagagtgttc tacagagttc
     2101 ttgaagtggt ggcctgactg cggctacact ggaaggacag ttttggtacc tgcgctccgt
     2161 gaaagccagt taccttcgga agaaagagtt ggtagctctt gattccggca aacgaaccac
     2221 cgttggtagc ggtggttttt ttgtttgcag ggcatgagat tacgacgaca tcgaagggat
     2281 aacaagagga tccgcttctt gaacccccct ccccacaaaa aaaacggcac actagcgcgt
     2341 aaagcgtcat caaaaaaact caaatagcgc tatatacaat cactttatta cgcccctctc
     2401 tcttagcttg ataaagagca tcgtcagcat ttttcagcaa aaaatcgatc ccaccaagag
     2461 gatcatcttg tgttgccaca ccagcagata cggtgacacg caagttatta gggaaaacag
     2521 tacgttcaat aacactccta atacgctccg caacacactc cgctgttacc acagatgtat
     2581 tcggcagaaa aacaacaaac tcttctccac caaaccgaca aataatatct tcactacggc
     2641 aacaagactt aataatctga gccaaagaaa caagcacctc atctccgacc ccatgcccga
     2701 aactgtcatt tatctcttta aaaaaatcaa catcaatagc aataactgaa gctcctgccc
     2761 catacttgtt attctgtatt ttagttgaaa accccaatct attgttcacg ccggtaaggg
     2821 gatctttaag cgccacctca tttaaagaat caaccctttc catcattacc ttaacatgcg
     2881 taaaaagtgc ctttttaagt cgatcagctt cggcatacca agcattgata gctttaatat
     2941 aaactagcga agctcctata tctttagcat tggtagatat agcaagcttt tctaaaggaa
     3001 gtgaaatact ggaggcaatg aaatatgaaa taagtgaaaa cacaataatt attataatta
     3061 gcagaacaag tacactttta gcataagcaa taagaatagt agtcacactg tcagcacgac
     3121 tataaaccaa cacattccaa gcagtatttt tcatactagc ataaccaaga agatatctat
     3181 gccccgaaga aaaaaatgcc ccctcacctc tatcagacgc agttaacttt gatttcaaat
     3241 catcagacat aaccataggc cgatcaacag cacttttatc gttattaaag atcacattcc
     3301 catcatcact aacaatgcta atttcagtgt tgttcttgaa aaaatgcctg ctcaaaacat
     3361 cgctaaataa actatgtttt tttagatata tagacccacc gacataccct atgtaattgc
     3421 cacccttatc ataaatagga tgtaacaaca aaataatcaa attcccagta actgatttga
     3481 acggtgaaga tatgaatggt ttcttttcct caatcgccaa tttgctggca cttgaattca
     3541 agcgggtacc aactaaatca agagattctg gcgaagtcgc cagaacaaca gcatcattac
     3601 taacaacaaa aactgaatta aacataccag actgcaatct taacctatcc gcctcatctt
     3661 taagtgagtc aatatcatta gttgatttta ttttttttgc tccatatgct aattcacctt
     3721 gagccatttc aatataacca ctcattacat ctgacatttt catagcgtac gatgaattag
     3781 tataaagcaa atctttttct atattttcgc gctgaaaaac cataataacg gcaatcagaa
     3841 aaatagaagt taacaaaatc ccaccaacgg acaaggttat gactatcgga cgaaggtgag
     3901 gctttttaat tctcataaaa atacactcca tataataaaa aatgccgcat ttaccataac
     3961 atcccttgcc cgcaccgccc cggacagctt cgccacgaac ggggcggcag tcagccaaaa
     4021 ccaacaacaa cccatccggt aaaccacccc gatcgcgaca gataaatttc acccaactgc
     4081 atagccatgc agccaggtga atgacggcag gattcagccc aaaaacagcg tgaaggcccc
     4141 gaaaaccggc aaatgcttct ccagccactc ccacagttct gccgccagat gcaggcagtt
     4201 aagtacagct tcaataacct taagcagttt cataattccc cctggtatag gcagtgatct
     4261 ttgcatcacg acgtatttag cggggaattt cgcagccctt cttcttgggg tttgtcacgg
     4321 gacggcaccc gtcagcccag cgtcggtcct ttgtaggttt tctgcggctg gcgctgcaac
     4381 tccaggcttt tctgatactc attctccctc tgccggagct cggtggcatt cagttcccgg
     4441 acaagcggcg cagctgctcg cttaaactca gcacctgcgt gctcaagtac gacacgcgct
     4501 cgttcagcac cgcgttctcc cgttgcataa gcccacacat gctccgccat tccgcgaagg
     4561 cgctctccca ttcgtccagc ctttgcgagt agtcctgctg tagctgctct aatgcgctca
     4621 gcaactgctt ttccagctct gtcattttcc gctactcctg tttcaggcgg atccatgtcg
     4681 tcccgtcgta ggggatggct tcggtttgca tccccggcgg caggataaga aacctccggc
     4741 cgtcgctgct ctcctgatag cgcactcccc acgtccgggc ggtcattttc gccagggtat
     4801 cttcctgctg acgaagatgc gtgtaattgt cggttatctg ctg
//
