LOCUS       NZ_CP056696             4096 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00626 plasmid pRHBSTW-00626_6,
            complete sequence.
ACCESSION   NZ_CP056696
VERSION     NZ_CP056696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148666
            Assembly: GCF_013734915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00626
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.344886308208x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734915.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:01:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,753
            CDSs (with protein)               :: 4,753
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 30 of 107
            Pseudo Genes (incomplete)         :: 77 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 18 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00626"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00626_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(282..551)
                     /locus_tag="HV249_RS24705"
                     /old_locus_tag="HV249_24705"
     CDS             complement(282..551)
                     /locus_tag="HV249_RS24705"
                     /old_locus_tag="HV249_24705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            633..866
                     /locus_tag="HV249_RS24710"
                     /old_locus_tag="HV249_24710"
     CDS             633..866
                     /locus_tag="HV249_RS24710"
                     /old_locus_tag="HV249_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            888..1115
                     /locus_tag="HV249_RS24715"
                     /old_locus_tag="HV249_24715"
     CDS             888..1115
                     /locus_tag="HV249_RS24715"
                     /old_locus_tag="HV249_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1645..1854)
                     /locus_tag="HV249_RS24720"
                     /old_locus_tag="HV249_24720"
     CDS             complement(1645..1854)
                     /locus_tag="HV249_RS24720"
                     /old_locus_tag="HV249_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1858..2343)
                     /locus_tag="HV249_RS24725"
                     /old_locus_tag="HV249_24725"
     CDS             complement(1858..2343)
                     /locus_tag="HV249_RS24725"
                     /old_locus_tag="HV249_24725"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2378..3082)
                     /locus_tag="HV249_RS25110"
                     /old_locus_tag="HV249_24730"
                     /pseudo=""
     CDS             complement(<2378..3082)
                     /locus_tag="HV249_RS25110"
                     /old_locus_tag="HV249_24730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3021..3344)
                     /locus_tag="HV249_RS24735"
                     /old_locus_tag="HV249_24735"
     CDS             complement(3021..3344)
                     /locus_tag="HV249_RS24735"
                     /old_locus_tag="HV249_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3453..3531)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          3607..4095
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(1536..3031)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggtatacga gagacaaatt aagaggtctt gaagtatggg cctgagagag gctacactga
       61 aagaacagtt ttggtttctg cggtctcgta tacccagtta cctggttaag cagttcccca
      121 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgtttt cagagcaaga
      181 gattacgact gtaacgacag gatcacaaga agatcattta ctcagagaga ctaactacca
      241 ctatgtttgc ctatcaacat acttaggaaa cattacttga gttaacgact ccaccttact
      301 tttagtatct cgcaatgaat cttccgaccg ccgttgtgtt gaagttctat tgcgccaccg
      361 gggtataaat cactttcgtt taactcaatt aaaagctcgt tcttgtgagc atcctctaaa
      421 taaaggtaat aacacccacc ataaaggttc tgcgtatctg gcacggcaac aaaagatttc
      481 tttccgaaca gtaaaacagc ctctttttct ccatgctctg tcagcaataa tcgaaattct
      541 tgtccattca atcctctttt cgcatgaaaa aacgacctat acttagatgg ttagtgacca
      601 tatccacttt acctttaagc agggaaccta ttatgaataa aaaaacgctg ttggcggcca
      661 tgatggtcgc gttggtgacc tttgccgctg ctggatgttc ttcaaaccaa gaaataaaaa
      721 ccacagatga tcgcactatc gttacagacg gcaaacccaa agttgacagt gatacgggat
      781 tagtatccta caaagatgcc catacaggcc gaaccgaaca gatcaaccgt gacaaaatta
      841 ccaacatgag tgaactagac aactgatacg gataaaagga gctggctttg aaaaagattt
      901 ttgtttcagt gttcgcagcg gcagttgcgc tttcagccct aaccggctgc acgcgcacaa
      961 gctatgccat ccacaccaac gacgggcgta caattgtcag cgacggcaag cctacagaat
     1021 cagactcagg cctgctgggc tataaagacg caaatggtgt caaacagcag atcaataaag
     1081 ccgacgttaa agaagtctct gaaattccgc attgaggcat cgctctctcc gaaaagggcc
     1141 gctaatgcgg cccttttctt tatctgcccc aacaacaggc aaaaaaagcc cccgctgggg
     1201 gccaaatacc tgcagtggct ggatagtact gcattcctga tgaaggagac gaataagtat
     1261 gggccggatt tttgcgtctg caggctcggt cgccacaatt tttccgtacc cgccaggcat
     1321 catgctttta ccataacatc ccttgcccgc acctctccgg acgggttcag cgcgaatgcg
     1381 gcgtcagtcc tgcaaaaccg acagtaaacc accccgcaaa ccgtgcattc tgcgcctgat
     1441 aaatctcacc cgactgcata gccatgcagc cgggtgaatg gcaccgggtt tcggcgcaga
     1501 tcctcaatga aagccccgtt ttatccggtt atcacagcga cggaccgtca tactcctgct
     1561 gacgctgcga tcgttccagc tccagcgctt tctggtgctg cgctgcgcgc tcctgctccc
     1621 ggtgtgcctc gatgtcgttc agccttacga caaccggcgc agctgctcgc ttaaactcag
     1681 cacctgcgtg ctcaagtccg tgacgcgctc gctcagcgct gcgttctccc gttgcataag
     1741 accacacatc gtccgccatt ccgcgaaggc gctctcccac tcgtccagcc ttttcgagta
     1801 gtcctgctgt agctgctcta atgcgctcag caactgtttt tccagttccg tcatgtgcta
     1861 tttccccgcc agtatcatcc agctcgaatc ctctccgaac cgtcccgcgt ccgggttcac
     1921 cctcacgcag cggcgtcgtt cctctccgca ggtcgtgagc tgcacgccgc tgttcctctc
     1981 ggacagcatg gcctgcgtgc tcttctgctc ccggatggtc gtgtaattgt cgagtatctg
     2041 ctgcccctgc caccatagaa tgcccgcact tgaggctatc agcagcacgg agaccaggac
     2101 gatggtcagc cacgtctggc tgaccatgcg cagcatccct ttcgtacgct ggctcatggc
     2161 tgaggacagc atccggtcgt ggtcgtggat ggcggcgctg attctcttct cgctttcgtt
     2221 cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca tctcgccggt
     2281 agtctgctgc tgcactttcg atttctgctc taagtccttc gccagcgtta aaagactgtt
     2341 catagatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatgc
     2401 tgctttttgt ggtgcgaacc acctcaaaac ctgcgctttc cagcgcctca gtgacgtcct
     2461 gacgcgtttt cagctccccg gacgaggcta aggccagtaa accccgcgta atcgcctgag
     2521 cggcttcctg cttcgtttcc ggcagcgcgg acggcgtgac cagtgcccgc cggttttccg
     2581 gcgcgttcgg gtcgtgcagc cccagactgc cgttcacgat ggtctgccag gcatcaatgc
     2641 gaggacggtc ggcgcggtca tagtatggct ggagccgttt gccggtcagc agctccgtgt
     2701 tcgggatcag gaagttcagc tccagccgcc ccttgtcccg gtgctcaacc cacagcacgc
     2761 tgtactggtc tttatcgagt ccgggcatca gaacccgctc gaagctcgcc atcagcgttt
     2821 cacgctgccc gggcggtaag tcctgttcgg caaaggacag caccccggag gtgtactttt
     2881 tggcgtaggg cgaggcgtcg ataagctccc ggacttcctc cggcttcccc tgaagaacgc
     2941 tggcaccgtc gcgctggcgg tctttcccca gcagataatc aaccgggccg gcaccgcctc
     3001 cgcgtccccg gggatgaaac ttaacgatca tcgtcagccc ccttttccag tacggcatgc
     3061 cgcagccgct cgagtccggc atcgatggcc atcagcgcgg cgacgacctg cacgcggtcg
     3121 agaccgctac tgccaccggc gttaacctgg cgggcaatct ggttgaggtt gttccccatg
     3181 ccggcaagct gacgaagcag cgccggcgag atcgagggaa gtttgccggc ccgggcgggc
     3241 ttctcgtcca ggcacgtctg ccgcatccag gccgcgagct gtttaccgtc gcagcgttcc
     3301 agcagacggc ggtgctcatc ttctgttacc cacatcgtga gcattttgct gcgtttgtct
     3361 gccaatccgg cctcctgata acgccgggac gggcgggaaa tcttttcccg gtcggcgcgg
     3421 tgttgggcaa gccgcaggcg cgtcagtggc atttagcggg tttcggggcg cagccctgaa
     3481 ccagtcacgt agcgctagcg gagtgtatac tggcttacta tggtggcacg acgggaatcg
     3541 ggatgaaata gtcagtcatg aaaactgtga gatgaggctc aggtgcgtaa gaggctgatg
     3601 cctgtcccgg tggatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc
     3661 tgcggcgagc ggtacggact cacggataaa gacgtggcgg ggaaatacgt gaatgcagga
     3721 ggggaagaaa ggatgcgccg ctgccggttt tccataggct ccgcccccct gacaagcatc
     3781 acagaatctg acgctcaaat cagtggtggc gaaacccgat gcggcttaaa gataccaggc
     3841 gtttccccct gatggctccc tcgtgctccc ccgcattcct gcctttcggt ttaccggtgt
     3901 cattccgctg ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgcgaaggc
     3961 agttcgctcc aggattgact gtatgcacga accccccgtt cagtccgact gctgcgcctt
     4021 atccggtaac tatcgtcttg agtccaaccc ggaaagacac gaaaaatcgc tactggcagc
     4081 agccattagt aactgg
//
