LOCUS       NZ_CP056695             4665 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00626 plasmid pRHBSTW-00626_5,
            complete sequence.
ACCESSION   NZ_CP056695
VERSION     NZ_CP056695.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148666
            Assembly: GCF_013734915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00626
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.344886308208x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734915.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:01:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,753
            CDSs (with protein)               :: 4,753
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 30 of 107
            Pseudo Genes (incomplete)         :: 77 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 18 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056695.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00626"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00626_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4425..4665,1..83)
                     /locus_tag="HV249_RS24680"
                     /old_locus_tag="HV249_24685"
     CDS             join(4425..4665,1..83)
                     /locus_tag="HV249_RS24680"
                     /old_locus_tag="HV249_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            292..>726
                     /locus_tag="HV249_RS25030"
                     /pseudo=""
     CDS             292..>726
                     /locus_tag="HV249_RS25030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            761..1246
                     /locus_tag="HV249_RS24690"
                     /old_locus_tag="HV249_24690"
     CDS             761..1246
                     /locus_tag="HV249_RS24690"
                     /old_locus_tag="HV249_24690"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1250..1465
                     /locus_tag="HV249_RS24695"
                     /old_locus_tag="HV249_24695"
     CDS             1250..1465
                     /locus_tag="HV249_RS24695"
                     /old_locus_tag="HV249_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1811..3367
                     /locus_tag="HV249_RS24700"
                     /old_locus_tag="HV249_24700"
     CDS             1811..3367
                     /locus_tag="HV249_RS24700"
                     /old_locus_tag="HV249_24700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          4139..4312
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(3663..4215)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          74..1569
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcggcgct gatggccatc gatgccggac ttgagcggct gcggcacgcc gttcttgaga
       61 aaggaacgga cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg cgccgggccg
      121 gtggactatc tgctgggtaa agaccggcag cgtgaaggcg ccaccgtcct gcaggggaag
      181 ccggaagaag tccgggagct gattgacgcg tcgccttacg ttaagaaata cacctccggg
      241 gtactgtcgt ttgcggaagc ggatttacct tccggccagc gggaaaagct gatggcaagc
      301 tttgagcggg ttctgatgcc cggacttgat aaagaccagt acagcatcct gtgggtggag
      361 catcgtgaca aggggcggct tgagctgaat tttctgatcc cgaacacgga gctgctgacc
      421 ggcagacggc tccagccgta ttacgatcgc gccgaccgtc cacgcatcga tgcctggcag
      481 accatcgtga acggcagact ggggctgcac gacccgaacg caccggagaa ccggcgggca
      541 ctggtcacac cgtctgcact gcctaaagcg aagcaggagg ctgcagaagc gattacacgc
      601 ggtctgcttg ctcttgcctc atccggggag ctgaaaaccc gtcaggatgt cactgaggcg
      661 ctggaaagcg caggttttga ggtggtccgc accacaaaaa gcagtatcag cattgccgac
      721 ccggacgggg ggcgaaacat ccgactgaag ggagccattt atgaacagtc ttttaacgct
      781 ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag aataccggcg agatgctgaa
      841 agccgcattc agcgagcacg agaaatctgt aaaagcggaa ctgaacgaaa gcgcgaagag
      901 aatcagcgcc gccatcctcg accacgaccg gaaactgtcc tcagccatga gccagcgcac
      961 gaaaggcatg gtgcgcatgg tcagccagac gtggctgacc atcgtcctgg tctccgcgct
     1021 gctgatcgcc tcgagcgcgg gcattctgtg gtggcagggg cagcagatac tcgacaacta
     1081 cacgaccatc cgggagcaga agagcacgca ggccatgctg tcagagagga acagcggcgt
     1141 gcagctctcg acctgcggcg agcagagacg ccgctgcgtg aaggtgaacc cggaagcggg
     1201 acggttcgga gaggattcga gctggatgat actggcgggg aaatagcaca tgacggagct
     1261 ggaaaaacag ttgctgagcg cattagagca gctacagcag gactactcga aaaggctgga
     1321 cgagtgggag agcgccttcg cggaatggcg gacgatgtct ggtcttatgc tacgggagaa
     1381 cgcagcgctg agcgagcgcg tcacgggctt gagcaggcag gtgcagagtt tgagcgagca
     1441 gctgcgccgg ttgtcgagcg actgaatgcc gtcgagctgc accgggagca ggagcgcgtg
     1501 gtgcagcatc agaaagcgct ggagctggag cgatctcagc gacagcggac gtataacagc
     1561 ccttcactgt gataacctga taaaacgggg ctttcggcgg acatctgcgc tgaaagccgg
     1621 agccattcac ccgactgcat agccatgcag ccgggtgaaa tttatctggc gcagaatgcg
     1681 cggtttagcg gagagtttgc tgccggtttt accgggctga cgccgcattt gttccgaacc
     1741 cgtccggggc ggtgctgata agggatgtta tggtaaatgc ggcatttttt attatatgga
     1801 gtgtattttt atgagaatta aaaagcctca ccttcgtccg atagtcataa ccttgtccgt
     1861 tggtgggatt ttgttaactt ctatttttct gattgccgtt attatggttt ttcagcgcga
     1921 aaatatagaa aaagatttgc tttatgctaa ttcatcgtac gctatgaaaa tgtcagatgt
     1981 aatgagtggt tatattgaaa tggctcaagg tgaattagca tatggagcaa aaaaaataaa
     2041 atcaactaat gatattgact cacttaaaga tgaggcggat aggttaagat tgcagtctgg
     2101 tatgtttaat tcagttcttg ttgttagtaa tgatgctgtt gttctggcga cttcgccaga
     2161 atctcttgat ttagttggta cccgcttgaa ttcaagtgcc agcaaattgg cgattgagga
     2221 aaagaaacca ttcatatctt caccgttaaa atcagttact gggaatttga ttattttgtt
     2281 gtcacatcct atttatgata agggtggcaa ttacataggg tatgtcggtg ggtctatata
     2341 tctaaaaaaa cataatttat ttggcgatgt tttgagcagg cattttttca agaacaacac
     2401 tgaaattagc attgttagtg atgatgggaa tgtgatcttt aataacgata aaagtgctgt
     2461 tgatcggcct atggttatgt ctgatgattt gaaatcaaag ttaactgcgt ctgatagagg
     2521 tgagggggca tttttttctt cggggcatag atatcttctt ggttatgcta gtatgaaaaa
     2581 tactgcttgg aatgtgttgg tttatagtcg tgctgacagt gtgactacta ttcttattgc
     2641 ttatgctaaa agtgtacttg ttctgctaat tataataatt attgtgtttt cacttatttc
     2701 atatttcatt gcctccagta tttcacttcc tttagaaaag cttgctatat ctaccaatgc
     2761 taaagatatc ggagcttcgc tagtttatat taaagctatc aatgcttggt atgccgaagc
     2821 tgataggctt aaaaaggcgc tttttacgta tattaaggta atgatggaaa gagttgattt
     2881 tctaaatgag gtggcgctta aagatcccct taccggcgtg aacaatagat tggggttttt
     2941 aagtaaaata cagaatcata agcgggattc agggtattca gttattgtaa ttgatgttga
     3001 tttttttaaa gagataaatg acagtttcgg gcatggggtc ggagatgagg tgcttgtttc
     3061 tttggctcat attattaatt cctgttgccg tagtgaagat gttgtttgtc ggtttggtgg
     3121 cgaagagttt gttgttttcc tgccgaatac atctgtggta acagctaagt gtgttgcgga
     3181 gcgtattagg agtgttattg aaagtactgt cttccctaat aacttgcggg tcaccatatc
     3241 tgctggtgtg gcaacacaag atgatcctct tggtaggatc gattttttgc tgaaaaatgc
     3301 tgacgatgct ctttatcaag ccaaaaggga ggggcgtaat aaagtgattg tatatagcgc
     3361 tatttgagtt ttttgatgat gctttacgcg ctagtgtgtc gttttttttg tggcgaagag
     3421 tgggcaagaa gcggatcctc ttgttatccc ttcgatgtcg tcgtaatctc ataccctgta
     3481 aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg gaattcagag ctaccaactc
     3541 tttcttccga aggtaactgg ctttcacgga gcgcaggtac caaaactgtc cttccagtgt
     3601 agccgcagtc aggccaccac ttcaagaact ctgtagaaca ctctactact ccgctccgtg
     3661 aatcctctta ctaatggcta ccgccactgg cgatttttcg tgtctgttcc gggttggact
     3721 caagatgata gttaccggga caacgtggta gtcgggctga acggggggtt cgtgcataca
     3781 gtccagcttg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa tgagataaac
     3841 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     3901 gggagccacc agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccaccactg
     3961 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     4021 cgccgcggcc ttctcgcttc cctgttacgt tccttcctgg catcttccag gaaatttccg
     4081 ccccacctgt aagccggcac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4141 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcgccgg tgccgccttt
     4201 tttctcctgc cacatgaagc acttcactga caccctcatc cgtgccaaca tattaagcca
     4261 gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgccaaaa
     4321 gcctgacgcg cctgcggctt gtccaacccc gtgccgaccg ggaaaggttt tcccgcccgt
     4381 cccggcgtta tcaggagacc ggtttgtcag agaaacggaa caagatgctc accatgtggg
     4441 tgacggagga cgagcaccgg cggctgcttg agcgctgtga cggcaggcag ctggcggcgt
     4501 ggatgcggca gacctgcctg gacgaaaagc cggcacgttc cggaaagctc ccctcaatct
     4561 cgccggcgct gctgcgccag cttgccggca tgggcaataa cctgaaccag atagcccgcc
     4621 gtgttaacgc cggcggcggt accggccatg acagggtgca gattg
//
