LOCUS       NZ_CP056694             4858 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00626 plasmid pRHBSTW-00626_4,
            complete sequence.
ACCESSION   NZ_CP056694
VERSION     NZ_CP056694.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148666
            Assembly: GCF_013734915.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00626
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.344886308208x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734915.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:01:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,753
            CDSs (with protein)               :: 4,753
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 30 of 107
            Pseudo Genes (incomplete)         :: 77 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 18 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00626"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00626_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4624..4858,1..89)
                     /locus_tag="HV249_RS24645"
                     /old_locus_tag="HV249_24650"
     CDS             join(4624..4858,1..89)
                     /locus_tag="HV249_RS24645"
                     /old_locus_tag="HV249_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            298..>732
                     /locus_tag="HV249_RS25025"
                     /pseudo=""
     CDS             298..>732
                     /locus_tag="HV249_RS25025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            767..1258
                     /locus_tag="HV249_RS24655"
                     /old_locus_tag="HV249_24660"
     CDS             767..1258
                     /locus_tag="HV249_RS24655"
                     /old_locus_tag="HV249_24660"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1265..1480
                     /locus_tag="HV249_RS24660"
                     /old_locus_tag="HV249_24665"
     CDS             1265..1480
                     /locus_tag="HV249_RS24660"
                     /old_locus_tag="HV249_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1952..2443
                     /locus_tag="HV249_RS24665"
                     /old_locus_tag="HV249_24670"
     CDS             1952..2443
                     /locus_tag="HV249_RS24665"
                     /old_locus_tag="HV249_24670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(2500..2928)
                     /locus_tag="HV249_RS24670"
                     /old_locus_tag="HV249_24675"
     CDS             complement(2500..2928)
                     /locus_tag="HV249_RS24670"
                     /old_locus_tag="HV249_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            3213..3641
                     /locus_tag="HV249_RS24675"
                     /old_locus_tag="HV249_24680"
     CDS             3213..3641
                     /locus_tag="HV249_RS24675"
                     /old_locus_tag="HV249_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          4338..4511
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(3869..4414)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          80..1572
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4625..4857
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="99.0"
                     /evalue="4.58e-43"
ORIGIN
        1 agattgtggc ggcgctgatg gccatcgatg ccggccttga gcggctgcgg cacgccgttc
       61 ttgagaaagg aacggacgat gatcgttaaa tttcatccgc gcggccgcgg cggtggcgcc
      121 gggccggtgg actatctgct gggtaaagac cggcagcgtg aaggcgccac cgtcctgcag
      181 gggaagccgg aagaagtccg ggagctgatt gacgcgtcgc cttacgttaa gaaatacacc
      241 tccggcgttc tgtcgtttgc ggaggccgat ctgcctcccg gccagcggga aaaactgatg
      301 gccagcttcg agcgggttct gatgcccgga ctcgataaag atcagtacag cattctgtgg
      361 gtggagcacg cggacaaggg gcgtctggag ctgaactttc tgatccccaa taccgagctg
      421 ctgaccggca ggcggctcca gccttactac gaccgcgccg accggccgcg catcgatgcc
      481 tggcagacta tcgtgaacgg cagactgggg ctgcacgacc cgaacgcgcc ggaaaaccgc
      541 cgggcgctgg tcacgccgtc cgggctgccg aaaacgaagc aggaggccgc agaagccatt
      601 acccggggac tgctgaccct tgcttcgtcc ggggagctga aaagccgtca ggatgtcact
      661 gacactcttg aaggtgcagg ttttgaggtg gtgcgcacca cgaaaagcag tatcagcatt
      721 gccgacccgg acggggggcg aaacatccga ctgaagggag ccatctatga gcagtctttt
      781 aacgctggcg aaggacttag agcagcaatc gaaagcgcag cagcagagta ccggcgagat
      841 gctgaaagcc gcattcagcg agcacgagca gtctgtcaga gcggaactga gcgcaagcgc
      901 gaggagaatc agcgacgcca tcctcgccca cgagcagagt atgagcgagg cgatggagaa
      961 aaaccggcgg agcgtactgc ggactgccgg gcggacatgg ctcaccatcc tgatggtgtc
     1021 agcgctgctg atcggcacga gcgggagcat tctgtggtgg caggggcagc agataaccga
     1081 caattacacg catcttcgtc agcaggaaga taccctggcg aaaatgaccg cccggacgtg
     1141 gggagtgcgc tatcaggaga gcagcgacgg ccggaggttt cttatcctgc cgccggggat
     1201 gcaaaccgaa gccatcccct acgacgggac gacatggatc cgcctgaaac aggagtagcg
     1261 gaaaatgaca gagctggaaa agcagttgct gagcgcatta gagcagctac agcaggacta
     1321 ctcgcaaagg ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtggtct
     1381 tatgcaacgg gagaacgcga tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct
     1441 gagtttaagc gagcagctgc gccgcttgtc cgggaactga atgccatcga gctccggcag
     1501 agggagaatg agtatcagaa aacactggag ctggagcgcc agccgcagaa aacctaccgc
     1561 gggccgacac tttaaaccat ccaggcagct gacaacccgc acgaagaggg gctcttattt
     1621 ctcccgctaa ataggttcgt gatcaaaaag gtcacgactt attcaggagc gggaaatgaa
     1681 gctgttaaat cttattgaat cattgcttaa ctgcctgtat ctggcggcgg agctgtggga
     1741 ctggctggag aagcatttgt cggtttttaa ggcattggcg cggattttag tgtgacttta
     1801 acatccatgc atctggctgc atgactatgc accagtgtaa tattcagcgg ctgcgtttct
     1861 ggtgtttttt cgttatgtct gtcgccactt tctcgcgtct ggcaccggaa acgcagccaa
     1921 ccccgtttca gcggtgcggg caacagatgt tatggtaaat caaccgtccg ttgataaagg
     1981 ttctgataat acccatgtag gtgatgttgt ttgcttttta tctacacact gcctgatgga
     2041 gctatttatg aatgctgatg aagttacacg aattcttgcg caatcgagtc aaaatgattg
     2101 gattgtggat gatgagacag gggcgtttac ttataaaaat gatttagcac tgcacattaa
     2161 gagagctgat tacgacgact acaactcgtt taatgagcct tgggctgttc gtcatcctga
     2221 tagtaatgcg tgttcagtcg agtacaccgt tcactatggt tcttcgtttg ttgatagaaa
     2281 aaccttggtt tccgtcgatg gtcatcgtgc aaccttgcct atgcctgcat ccgcaacgga
     2341 tctaaacgtg tcatcggctg atgtgaattt cgccaaaatt gtaaacattg gcgatcgcgt
     2401 tgatgaatac attcaacgtt ctggtcttac gattaagccc taatttaacg ataagcccgg
     2461 cctccaaatg cgggctgttc ctgcatgtaa ccagcataac taatcggctt ttttccttac
     2521 tgtatatatt ggctttttat gtcgcggctt tcttacttgc tcggacacag aaggaccaat
     2581 tatgtctaaa taatctacaa gctttgtaag gtcgtatatg ttgcggccac catatcggtt
     2641 gtcgcttttt gatgtcggtt ttttatctaa gagattggct ttttctaggt cattaatagc
     2701 cctttgcacc gtccttactg acacccccat ccgtatagcc aggctctcca ttgaggggtg
     2761 cggccactcc tgtagattcc accagtgtac tatgagattc agcaaggtat tgaatgctac
     2821 agggctcaat ttcttctcat tttgtataaa aaacaacaca gtaggtactg cagtccatcc
     2881 taatgacaga cctccagcct tccatttttt ttctattgcg taactcattg attatcctgt
     2941 aaatattttt tttgagatat atataatata agtattcata tgaatagggt gacaccagcg
     3001 ccattattac acaactataa aaggctaatc tgagaacaga aaaacatttc tatctgcatt
     3061 aaatacggag ttagtgatat gaaaaccaaa caacatcatg atcttgtaaa agatactgaa
     3121 atatttggtg tatccttgtc tctgttgatt tccagagcga atgagagatc tgttacaatg
     3181 ccatctggtc taagtaggga gcagagaaga gcatgggcag cggatcaagc acgcaaaatc
     3241 cacaattgaa tgttgtctct ttctctgaga cattcaatct ttattacaca tcccgctttt
     3301 ctcaaaaaac actcgataaa atcgatgact tcatcgaaca tttcgagaag tacaatcttt
     3361 ttggcgatgc cttaaaaggc catccggctt ggattggcaa ggtatcgcca tcttggaacg
     3421 tgcctgacag ttacgaaaac aaagaagcta ttgaagaaca tgccagggct aataaattgt
     3481 ggcatgccca tataggcgat cccgttttca tcgatacatt tcacgggaag tacaaagtct
     3541 ctgattgggt catccacttc caacgtctag cgccgaacca tataaaactg ttagagcttg
     3601 ggtatcatga ccctatgcaa ctcccccaaa atttcgaatg atctcttgtt cggatgaatg
     3661 atcccttgag attcgttgac cctcgtcgta atctcttgcg gtgaaaacga aaaaaccacc
     3721 cggcaaggtg gctttttcga aggttcgcaa gagttgacgc tcttttgaac cgcggtaact
     3781 ggcttggagg agcacagtca ccaaaactgt cctttcagtg tagccttatc gcacccgaaa
     3841 cttcaagact ctcctcctct aaacctgtta ccagtggctg ctgccagtgg cgctttgtcg
     3901 tgtttttccg gattggactc aagttgatag ttaccggata aggcgcagcg gtcggactga
     3961 acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcccgga actgagtgtc
     4021 aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc
     4081 aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc tttaagtcct
     4141 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     4201 agcctatgga aaaacggctt cgccgcggcc ttatcgtttc cctgttaagt ttcttcctgg
     4261 catctaccag gaatttccgc cccattcgta agcccacacc gctcgccgca gccgaacgac
     4321 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcgca tattctgctg
     4381 acgcgccggt gccgcctttt ttctcctgcc acatgaagca cttcactgat tcccgcaccc
     4441 gtgccaatat aataagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
     4501 gccccgaaac ccgccaaaag cctgacgcgc ctgcggcttg tccaaccccg cgccgaccgg
     4561 gaaaggcttt cccgcccgtc ccggcgttat caggaggccg gtttgtcaga gaaacggagc
     4621 aagatgctca ccatgtgggt gacggaggac gagcaccggc ggctgcttga gcgctgtgac
     4681 ggcaggcagc tggcggcgtg gatgcggcag atctgcctgg acgaaaagcc ggcacgttcc
     4741 ggaaagctcc cctcgatctc gccggcgctg ctgcgccagc ttgccggcat gggcaataac
     4801 ctgaaccaga tagcccgccg tgttaacgcc ggcggcggca ccggccatga cagggtac
//
