LOCUS       NZ_CP056690             4163 bp    DNA     circular CON 04-APR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00634 plasmid pRHBSTW-00634_9,
            complete sequence.
ACCESSION   NZ_CP056690
VERSION     NZ_CP056690.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148669
            Assembly: GCF_013734595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00634
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.4069515900754x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734595.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:43:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,498
            CDSs (total)                      :: 6,382
            Genes (coding)                    :: 6,176
            CDSs (with protein)               :: 6,176
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 79 of 206
            Pseudo Genes (incomplete)         :: 151 of 206
            Pseudo Genes (internal stop)      :: 45 of 206
            Pseudo Genes (multiple problems)  :: 57 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056690.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00634"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00634_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            35..226
                     /locus_tag="HV252_RS32275"
                     /old_locus_tag="HV252_32280"
     CDS             35..226
                     /locus_tag="HV252_RS32275"
                     /old_locus_tag="HV252_32280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            226..648
                     /locus_tag="HV252_RS32280"
                     /old_locus_tag="HV252_32285"
     CDS             226..648
                     /locus_tag="HV252_RS32280"
                     /old_locus_tag="HV252_32285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1767..1958)
                     /locus_tag="HV252_RS32285"
                     /old_locus_tag="HV252_32290"
     CDS             complement(1767..1958)
                     /locus_tag="HV252_RS32285"
                     /old_locus_tag="HV252_32290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            2200..3310
                     /locus_tag="HV252_RS32290"
                     /old_locus_tag="HV252_32295"
                     /pseudo=""
     CDS             2200..3310
                     /locus_tag="HV252_RS32290"
                     /old_locus_tag="HV252_32295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(3680..3793)
                     /locus_tag="HV252_RS32955"
                     /old_locus_tag="HV252_32300"
     CDS             complement(3680..3793)
                     /locus_tag="HV252_RS32955"
                     /old_locus_tag="HV252_32300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(3681..4057)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.825"
                     /coverage="92.0"
                     /evalue="3.51e-178"
ORIGIN
        1 tctgacattg agcactgacg ctctgaggcc ctttatgacg aaacaacaga aaacagcgct
       61 gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc ttaacgagct
      121 tgatctcgat gttgaggccg acctttgtga gaaactccac gatgatgcgg agcatctttt
      181 ccgtacactg tcctcgagac tggatgaatt acaggatggc aattgatggc cagagtgtct
      241 attagtgaag cggcacggct tgttaaggtt agccgtccca caatttacaa gatgattaac
      301 agtgggaagt taagttacac ctctgttgta aagcacggga aggctattaa agttattgat
      361 acttcagagc ttattagagt ttttggttct cttgatggtg tcatcgatac tgtaaaatat
      421 gatgtaaaaa gtgacgcaga atctacaagt attaacagcg ttggtttaca tgatttacaa
      481 catagaatcg ctctacttga agctgaaaac gatggtctta aaggggctgt taaggctcga
      541 gatgagcata ttgattccct tcgtcaggct atgcagcttc tggagcacaa gcgcgagcct
      601 tcttcaccac cccattcccc gtggtggaaa ttctggaaaa aatcctgatc ctgccatgct
      661 tgagttttcc tctccggcca tcccagggag ttcctgaacc ttcccaaaaa tcgccagatc
      721 tctgaacgct cctcacgact aaaaataccc acacactacg caaaattaaa attttgcaga
      781 ttccttattt aaaacaattg gttaatgaaa aattaatgtg cagtgatgtg ttacaaagtg
      841 cagatccaag tcgtggacga ctctatggcg aatcgtttac gaattgccca cgattcatat
      901 gtgaatcacg ctttattatc atgttgttat gatgaatcga ctcttgattc aattcatcaa
      961 tttgggcgtt ttttgttcat ggtgactctt gattcaattc acgattcgaa tcgccggtga
     1021 atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc
     1081 gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgcct cacggccacg
     1141 caataaattg cgtccccgtt caccgttttc ggatcagaag aacgccggaa acaggactta
     1201 ccctggctga atggcgtgaa tttccgcgct atgcacttgc gcgcatactc atgcatgccg
     1261 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
     1321 gtttcccgtc agaaacgccc tgaggccgtt ttagcggtgc gcgtaagaag acgttatggt
     1381 aaatccaaaa atgctggcat tcgcaattca gtatatcggt acgcagcata ataatggggg
     1441 ttaatttcca acgtatgagc catcaggtgc taaggttgct tccgaaccac caacgtatga
     1501 gccatcaggt gctaaggttg cttccgaacc actaacgtac gatccatcag gtgctaaggt
     1561 tacttgggat atcgcggtta gtggtaatgc tgacatagcc attaaaacgg caccaagaga
     1621 taatgctctc atcactatta ctcctgttaa tatcaaaaat agaattgaat cgttcagcgg
     1681 tgccgaacgc cggttccagc cgcgggattc gtgatcggga tgttatagta aattgatctc
     1741 ttgattttga gtgcccttac tcgaaatcag gttgctattt tcagtgtcgc ggctactgct
     1801 tgtacccccg gaagctcctg aagattatta ttgatagcaa actttatctc ttcaaagctc
     1861 gcgttaatca tgagcttctc atccacgcca cgtaaaaata ttattgtttt tgcattacca
     1921 catgatttca taaagataat atcatctttt tttagcatga tactgtcgtt acttagtgta
     1981 gtgagataaa gcatagccac tgtttttgtg tgtttttgtt aaggctgtta agattattcc
     2041 taaccactga ttttgtacat atcatcaaaa aaaataaaaa aactaatgcc cccgtggtgg
     2101 gtatataaca aatcacgggg agcggagctg acctgctccc cgttgattaa tacaccgcga
     2161 tgttagtaat gtcttcataa gccacatgag gacatcctca tgaagaagcg tttttccgac
     2221 gaacagatca tcagtattct ccgcgaggcc gaagccgggg tttctgcccg tgagctctgc
     2281 cgtaagcacg ccatttccga cgccatcttt tacacctggc gtaagaagta tggcggtatg
     2341 gaggtgcccg aggttaagcg ccttaagtcg cttgaggaag agaacgccag actcaagaaa
     2401 ctgcttgccg aagccatgct ggataagcag gcgcttcagg tggctctggg gcgaaagtac
     2461 tgacgacaga ccagaagcag gaagccgttg agtttatgtg tgatgcgacc ggtctgtcgc
     2521 aacgtagtgc ctgcaggctt acaggtttgc ccctgtcgac ctgccgctat gaggctcagc
     2581 gttcggcggc tgatgcgcat ttatcactga gctggcactg gagcgcaggc gttttggcta
     2641 ccgacgcatc tggcagttac tgcgccgtga aggccttcat gttaatcaca agcgcgtgta
     2701 ccgtctttac caccttaacg ggctgggtgt aaaacgcaga cgacgtcgta aaggtctggc
     2761 aacagaacgt ctgccgctgc tccgcccgga ggcgcccaac ctgacctggc cgatggattt
     2821 tgtcatggac gcgctggcca ccggtcgcag gatcaagtgc ctgacctgcg tggacgactt
     2881 cacgaaggag tgtctgacaa ttaccgccgt cttcgggatt tcaggcgttc aggtcacgcg
     2941 aattctggac agcattgcac tgtttcgcgg ctatccggcg acgataagaa ctgaccaggg
     3001 gccggagttt acctgcagag cactcgacca gtgggcttat gagcatgggg tggagctacg
     3061 gcttatccag ccgggcaggc caacacagaa cggatttatt gaaagtttta acggacgatt
     3121 cagggatgag tgcctcaatg agcactggtt cagcgatatc gttcacgcca ggaaaacgat
     3181 taatgactgg cggcaggatt agagcgagtg tcgtccacat tcatcgctga actaccagac
     3241 tccggctgaa tttgcggcgg gctggcgaaa cgggaaatat gaagaaaaac gaaccgacat
     3301 tactaactga aggttgtatc taatcctggg gggcaggtca accgcgttgg atgattgtgg
     3361 atcatgatta tatatgtttt tttcttatca gaaacaatgg gttaaatagt tttattttga
     3421 tggttggtgt cgcggagtgg tgctggtttc acttcagggg taggcatatg gaacaggatc
     3481 ctcttgagat cctttcttta gcatcgtaat ctcttgctca gtaaacgaaa aaaccgccct
     3541 gcaaggcggt tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc
     3601 ttggaggagc acagtcacca aaactgttcg tttagtttag ccttaaccgg cgcataactt
     3661 caagtccatc tcctctaaat cagttaccag tggctgctgc cagtggcgct tttgcatgtc
     3721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcagtcg gactgaacgg
     3781 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     3841 gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aatggcagga
     3901 acaggagagc gcacgaggga gccatcaggg ggaaacgcct ggtatcttta tagtcctgtc
     3961 gggtttcgcc accactgatt tgagcgtcaa attctgtgat gcttgtcagg ggggcggagc
     4021 ctatggaaaa acggccgctg tgctgccttc tccctctccg agctgtctgc tctatgtcgt
     4081 tatgtgcgcg ttgcgaccgc tcgccgcagc cgaacggcag gagcgtagcg actgagtgag
     4141 cgaggaagcg gaaaagagat ctg
//
