LOCUS       NZ_CP056689             5754 bp    DNA     circular CON 04-APR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00634 plasmid pRHBSTW-00634_8,
            complete sequence.
ACCESSION   NZ_CP056689
VERSION     NZ_CP056689.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148669
            Assembly: GCF_013734595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00634
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.4069515900754x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734595.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:43:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,498
            CDSs (total)                      :: 6,382
            Genes (coding)                    :: 6,176
            CDSs (with protein)               :: 6,176
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 79 of 206
            Pseudo Genes (incomplete)         :: 151 of 206
            Pseudo Genes (internal stop)      :: 45 of 206
            Pseudo Genes (multiple problems)  :: 57 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056689.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00634"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00634_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            370..888
                     /locus_tag="HV252_RS32235"
                     /old_locus_tag="HV252_32240"
     CDS             370..888
                     /locus_tag="HV252_RS32235"
                     /old_locus_tag="HV252_32240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001445155.1"
                     /translation="MSEKAKKVGRRSLSKRRESLIKELWGDELSELNIWNRKEHDGFTT
                     IPRTLNYINRIIDYLAGAGSPVSQTYLSLWCRVFDEGFVEIRDKDALAFESGFSGQRAV
                     TTWLGRMKKLRELGLISTKPGTSGEFHYVIMLNPLYVIKKSYESNGWARDERYNALFSR
                     MQEVGAEWE"
                     /name="hypothetical protein"
     gene            885..1448
                     /locus_tag="HV252_RS32240"
                     /old_locus_tag="HV252_32245"
     CDS             885..1448
                     /locus_tag="HV252_RS32240"
                     /old_locus_tag="HV252_32245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999222.1"
                     /translation="MNDMKAVRHRYKQRYDFAASEVVGAFDELRSLDWSLEIASLSDVE
                     MSKRWIGAVDPNGVARLPPNGGWDWVKLYNGYLKNPSAFVCAIKSEGKLCALIGGGVSD
                     SKVVTKLHYLESAPYDTALSGYALDISVTYVVTVANAMGSEYSAIYEPNSKVREIAIND
                     YGFSEDNLFGTSKDFALYLPMSRI"
                     /name="hypothetical protein"
     gene            complement(1906..2052)
                     /locus_tag="HV252_RS32750"
     CDS             complement(1906..2052)
                     /locus_tag="HV252_RS32750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     gene            2281..2484
                     /locus_tag="HV252_RS32245"
                     /old_locus_tag="HV252_32250"
     CDS             2281..2484
                     /locus_tag="HV252_RS32245"
                     /old_locus_tag="HV252_32250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000415556.1"
                     /translation="MEKRQRRILFQPFLISAKSLYPAIRELATPAAVSRQERSDRVSEE
                     ASYHRNLHFLLYALQLHFSPAT"
                     /name="hypothetical protein"
     gene            2693..3016
                     /locus_tag="HV252_RS32250"
                     /old_locus_tag="HV252_32255"
     CDS             2693..3016
                     /locus_tag="HV252_RS32250"
                     /old_locus_tag="HV252_32255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3225..>3659
                     /locus_tag="HV252_RS32780"
                     /pseudo=""
     CDS             3225..>3659
                     /locus_tag="HV252_RS32780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3694..4179
                     /locus_tag="HV252_RS32260"
                     /old_locus_tag="HV252_32265"
     CDS             3694..4179
                     /locus_tag="HV252_RS32260"
                     /old_locus_tag="HV252_32265"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015059929.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4183..4398
                     /locus_tag="HV252_RS32265"
                     /old_locus_tag="HV252_32270"
     CDS             4183..4398
                     /locus_tag="HV252_RS32265"
                     /old_locus_tag="HV252_32270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4597..5148)
                     /locus_tag="HV252_RS32270"
                     /old_locus_tag="HV252_32275"
     CDS             complement(4597..5148)
                     /locus_tag="HV252_RS32270"
                     /old_locus_tag="HV252_32275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     Typing          2405..2589
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.17"
                     /coverage="86.0"
                     /evalue="5.83e-49"
     Typing          complement(1944..2459)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.211"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3007..4502
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.385"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actacgacgt cgaggaatca tttaaaaaga cgaagcccgc ttatgtgggc tttttcattg
       61 cgcattcagg gcaccccttc ccccccttaa accccctaag aaccccattc agcacagcga
      121 gcacaacgat tacattagag tacattgaga acagcgagta caaaatagcg cataactaat
      181 tgaaaaataa aaccgtaatt gtaaaaaata ttacatctca atatttgtat tgacaaaaaa
      241 aaagaatcat atcaaatgag atgttggtta atgatgtgct tggtttttgt gttattacac
      301 ataactcatt gaatataata ggttttttgt tgataatatt ttcatgttga attgactttt
      361 gggggataaa tgtcagagaa ggcaaaaaaa gtaggtaggc gctctctatc aaaaagaaga
      421 gaaagtctaa taaaagagct ttggggggat gagctttccg aacttaatat atggaacagg
      481 aaagaacacg atggatttac aacaatccca agaacattga attatatcaa taggataatc
      541 gattaccttg ctggcgctgg atctcctgtg tcacagacat acctcagttt atggtgtcga
      601 gtttttgacg agggattcgt cgaaattagg gataaagatg ctctggcatt tgagtcaggg
      661 tttagtgggc agcgagcggt gactacttgg ctaggaagaa tgaaaaagct acgggagctg
      721 ggactgatat caacgaagcc tggtacttct ggcgagtttc attatgtaat catgttgaat
      781 ccactttacg taattaaaaa gtcatatgaa tcaaatggat gggcaaggga tgagcgttac
      841 aacgctctat tttctaggat gcaggaagtg ggggcggagt gggaatgaac gacatgaaag
      901 ctgttcgtca ccgatataag cagagatatg attttgctgc gtcagaagta gtgggggcgt
      961 ttgatgaact tagaagtttg gattggagtt tagaaatcgc atctttaagt gacgtggaaa
     1021 tgtcaaagag atggattggg gctgttgatc ctaacggtgt tgcaagacta ccacctaacg
     1081 gtggatggga ctgggttaag ctctacaacg gctatttgaa gaaccctagc gcattcgtct
     1141 gtgccattaa atcagaaggc aagctatgcg ctctgattgg tggcggggtt tctgatagta
     1201 aagtggtaac taagctgcat taccttgaat cagcccctta tgacacggct ctttcaggat
     1261 atgcactgga tatttctgtt acgtatgttg ttactgtcgc gaatgcgatg ggaagcgaat
     1321 actcagccat ctatgagcca aatagcaaag tcagagagat agctataaat gattatggat
     1381 ttagcgagga caacttgttt gggacaagta aagatttcgc tctgtatcta ccaatgagcc
     1441 ggatttaacc ggctcattta tcctaccagt acgccgtaga gtttttctgg ctgttggttt
     1501 gatcggcctt gctctgctcg tcttgctttc acgcgctggc gaaatgctcc ggctgtgcca
     1561 gaagagcatg gttccgtcgc ttttttaagt tcgaccacgt gagctaacat attatttccg
     1621 ttttttcgtg agttagtaca aagcatatga ggtgacggat acgtcaactt catagtaact
     1681 gtactgtatt gtagaagaga tgcgcaagtc gcagaattaa tgcgaccctt cattgacgag
     1741 gcgaagagta aacgcgtatc ttctttgaaa acgaaaaaac cacccggcaa ggtggttttt
     1801 cgaaggttca ttaaaccggc aagtttatga accgtggtaa cagggtgtac aagaccactg
     1861 ccaccaaatt cgtccttcca gtttagccgt agttgggctg atacttcaag aactctgcgt
     1921 tagtaatctc ttgtacatcc tcttaccgtt ggctgctgcc agtggcgctt ttgcatgtct
     1981 ttccgggttg gactcaagat gatagttacc ggataaggcg cagcagtcgg actgaacggg
     2041 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     2101 tggaatgaga aaccgcggcc ataacagcgg agtgacaccg gtaaaccgaa aggcaggaac
     2161 aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata gtcctgtcgg
     2221 gtttcgccgc ctctgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     2281 atggaaaaac ggcaacgccg catccttttt cagcccttcc tgatttccgc taagtctctt
     2341 tatccagcca ttcgtgagct ggcaacgccc gccgcagtct cacggcagga gcgcagcgac
     2401 cgagtgagcg aggaggcgtc atatcaccgt aacctccatt ttctcctgta cgccctgcag
     2461 ctgcattttt cccctgccac atgaagcact ttcattaagt gttactccgc cgtaacatat
     2521 tgagctagta tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaacct
     2581 gctaaaacca ctgacgcgcc tgcggcttgt ccaacaccgc gccgaccggg aaaagatttc
     2641 ccgcccgtcc cggcgttatc aggaggctgg attggcagac aaacgcagta aaatgctcac
     2701 gatgtgggtg accgaagatg agcaccgccg tctgttggaa cgctgcgacg gtaaacagct
     2761 tgcagcctgg atgcgccaga cgtgcttgga cgagaaaccc gcccgcgccg gcaagcttcc
     2821 ctcgatctcg ccggcgctgc ttcgtcagct tgccggcatg gggaacaacc tcaaccagat
     2881 tgcccggcag gttaacgccg gtggtggcag cgggcacgac cgcgtgcagg tcgtcgccgc
     2941 gctgatggcc atagatgccg gactcgagcg gctgcggcat gccgtgctgg aaaagggtgc
     3001 agacgatgat cgttaagttt cacccgcgag ggcgcggcgg tggcgccggt ccggtggatt
     3061 atctgctggg gaaagaccga cagcgcgacg gcgccaccgt tttgcagggg aagccggaag
     3121 aagtcaggga gcttatcgac gcctcgccct acgcaaaaaa gtacacatcc ggcgtcctgt
     3181 cctttgccga aaaggattta ccgcccgggc agcgcgaaaa gctgatggcg agctttgaac
     3241 gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt gagcaccagg
     3301 ataaggggcg gctggagctg aacttcctga tcccgaacat ggagctgctg actggcaggc
     3361 gtctccagcc gtattacgac cgcgccgacc gtccgcgcat tgatgcctgg cagaccatcg
     3421 tgaacggcag gctggggctg catgacccga acgcgcctga aaaccggcgg gcgctggtca
     3481 cgccgtccgc gctgccgaaa acgaagcagg aagccgctca ggcaattaca cggggtttac
     3541 tggccttagc ctcgtccggg gagctgaaaa cccgccagga cgtcactgag gcgctggaaa
     3601 gcgcaggttt tgaggtggta cgcaccacaa aaagcagcat cagcattgcc gacccggacg
     3661 gggggcgaaa catccgactg aagggagcca tctatgaaca gtcttttaac gctggcaaag
     3721 gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgagatgct gaaagccgca
     3781 ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgagaa gagaatcagc
     3841 gccgccatcc tcgaccacga ccggaagctg tcctcagcca tgagccagcg cacgaaaggc
     3901 atggtgcgta tggtcagcca gacgtggctg accatcgtcc tggtgtctac actgctgaca
     3961 gcatcgggcg caagcattct atggtggcag gggcagcaga tactcgacaa ttacacgatc
     4021 atccgggagc agaagagcac gcaggccatg ctgtccgaga ggaacagcgg cgtgcagctc
     4081 tcgacctgcg gagagcagag acgccgctgc gtgagggtga acccggaagc gggacggttc
     4141 ggagaggatt cgagctggat gatactggcg gggaaatagc acatgacgga gctggaaaaa
     4201 cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg
     4261 gagaacgcct tcgcggaatg gcggacgatg tctggtctta tgcaacggga gaacgcggcg
     4321 ctgagcgagc gcgtcacggg cttgagcagg caggtgcaga gtttgagcga gcagctgcgc
     4381 cggttgtcac aaggctgaac gtgatagagg cacatcgtga gcaggaacgg gcggcgcagc
     4441 atcagaaagc gctggagctg gaacgatctc agcgacagcg aacgtacaac ggtccgtcac
     4501 tttgaatctg tgcgccggaa aagcatgggc ttgctgtttc tggcgtacag gttcagttct
     4561 agaacatgag gctgctatct tgttgttcac gcttcatcag atcttgatgc ttattgttat
     4621 ggctgagcta tcaatccctg ttgcgttgct aatatccttt ttggctcttt caattgcctc
     4681 gttaagggag agtttttctg ttttctcttc agccagacca ggaatcactg tattaggaac
     4741 cgatggggat gaaggatgga atttcttcca ttccagttgt tccggtgtta ccccaatacg
     4801 taaaaaagct tcctgtgcgc tcaggtaagc aaccggatag cgctgaccat cggttagcat
     4861 tttccatgct tttggcgtgt gaatttctgc gtattcatca aaggttatga cgttcctgtc
     4921 gtcgtaatca gggcttttaa aacacccggt aattttggcg atcaggaatg ctgtgccgtg
     4981 ttctaggtct tttactgccc acgtttccct tctgtttctt actgctatga ggtaatcggc
     5041 atcgttgagc ctgtctgttc tggcatgcca gtagcctgac cccccctcag ttctcattat
     5101 gttcaaatcc ttcccggtaa aaaccacaac agctttcata tctttcatat agaaaccctt
     5161 aataggtgag taaatttcat ttttttatga aatctatacg atagaattcg tgttgtaaat
     5221 gttttttata cgataaaata cagcccccta cctcttttac tgttagtggt aaggctttct
     5281 gtttgatttt ttgataaacc ggttaaaaca ggggcttgga cgaggatata cattgaaagc
     5341 atgagccatt cacgtggctg catggctatg cagtcgcgtg aaatttatca ggcgcagaat
     5401 gcgcggttta gcggggagtt tactgccggt tttaccgggc taacgccgct ttcacgctga
     5461 acccgtccgg agcggtgcgg gcaagggatg ttatggtaaa tggtttctga ggtgtaagcg
     5521 aaaactcgag ttttgacgcc attctgagta ggtaatttgc agacggaata aatactggcg
     5581 agcaataagc tcatcaacaa aaaatggaga tcctaagatg gatagagaaa ccctatacct
     5641 actcaaaacg ttagaccata acaatgcctt gcttgacgat attatccgaa gaaagatggg
     5701 taggtactac aacacgaaga ttttgcgtga ttcctgtgat gctattgaag ccga
//
