LOCUS       NZ_CP056681             1329 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00642 plasmid pRHBSTW-00642_6,
            complete sequence.
ACCESSION   NZ_CP056681
VERSION     NZ_CP056681.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148671
            Assembly: GCF_013734275.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00642
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.032019356373x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734275.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,011
            Genes (coding)                    :: 4,900
            CDSs (with protein)               :: 4,900
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 34 of 111
            Pseudo Genes (incomplete)         :: 85 of 111
            Pseudo Genes (internal stop)      :: 19 of 111
            Pseudo Genes (multiple problems)  :: 24 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056681.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00642"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00642_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1124..1329,1..322))
                     /locus_tag="HV254_RS25485"
                     /old_locus_tag="HV254_25490"
     CDS             complement(join(1124..1329,1..322))
                     /locus_tag="HV254_RS25485"
                     /old_locus_tag="HV254_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271729.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_045282382.1"
                     /translation="MNKKLSDHETQGSHGETDNFNFVQLSRGYLQQMRKLARKSPIAHE
                     ILYYLVEHMGRTTNAVVCSYTTLCEITGVSRPTVGRAIKLLKEDNWIDTVKIGNSSAYC
                     VNEKVFWQSGRNQRKYAIFSATVIASASEQESDYAEKSKHKLTYIPIIESGEAAIVGSD
                     ELPPPDQKDLDL"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 tttcattcac gcaataagcg ctggaattcc ctatttttac cgtatctatc caattatctt
       61 cttttaaaag ctttatagct ctacctaccg ttggtctgct taccccagtg atctcacaga
      121 gcgttgtgta gctgcataca acagcgttcg tagtcctgcc catgtgttct actaggtaat
      181 ataaaatttc atgcgctata gggcttttac gggccaattt ccgcatttgt tgtaggtagc
      241 ctcgagagag ttgcacaaag ttaaaattat ctgtttcacc gtgagaccct tgagtctcat
      301 gatctgatag ctttttgttc atatatatcc ctactaaact cacaatttga cataaagact
      361 atcattaaat gagcttggtt gtaaaaggta gtctcaccag ctatgagact caccagtctc
      421 atagctggtg agactggtga gtctcatata acaataaaaa acaatgactt atatttacat
      481 tctttctata tctaataagg gggggatacc aaaagtcgct tgggggtggt tagcgggttt
      541 cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgtatactgg ctacatttta
      601 tttggcctct gagcgtaagc agaatctaca cgttttgatg atctggtgcc tcctcgctta
      661 cttggtcgct acgctcctgc cgtaagactg cggcgggcac tgctgattca gtattggcgg
      721 cctgataccg tcaatgacga gcttgcgagt tcatttatca ttgacggcgc actgctgggt
      781 aaactagcta tggtgcaagg tggcgcgtag cgtcggcttg cacttggtaa gcggtttttg
      841 tttttttagt atattaacct gtgcaagctg aggataatgt tttttctcgg tatcaccgtc
      901 gctgcgcaat agcactgcac atattaatat taacccaagt ctttttattt ttattttaac
      961 tttcatttca ctctcctatt attattatta ttccattgct ggaatggaat atatatattt
     1021 aaaaaatagg aaaagcaaat atatttcata taaaaatgaa atatatttta tgatgccttt
     1081 ttgttttatt ttatattgtt gtttttaatg gtttttttat attttataaa tcaagatctt
     1141 tttggtcggg tggtggtagt tcgtcactcc caactattgc agcctcccca gactctataa
     1201 taggtatgta tgtaagtttg tgcttggact tttcagcgta atcgctttcc tgttcagatg
     1261 ctgaagctat tactgttgct gaaaatattg cgtatttcct ctggtttctt cctgattgcc
     1321 aaaatactt
//
