LOCUS       NZ_CP056680             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00642 plasmid pRHBSTW-00642_5,
            complete sequence.
ACCESSION   NZ_CP056680
VERSION     NZ_CP056680.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148671
            Assembly: GCF_013734275.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00642
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.032019356373x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734275.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,011
            Genes (coding)                    :: 4,900
            CDSs (with protein)               :: 4,900
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 34 of 111
            Pseudo Genes (incomplete)         :: 85 of 111
            Pseudo Genes (internal stop)      :: 19 of 111
            Pseudo Genes (multiple problems)  :: 24 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056680.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00642"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00642_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2488..2495,1..211))
                     /locus_tag="HV254_RS25470"
                     /old_locus_tag="HV254_25475"
     CDS             complement(join(2488..2495,1..211))
                     /locus_tag="HV254_RS25470"
                     /old_locus_tag="HV254_25475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1419..1646
                     /locus_tag="HV254_RS25475"
                     /old_locus_tag="HV254_25480"
     CDS             1419..1646
                     /locus_tag="HV254_RS25475"
                     /old_locus_tag="HV254_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2012..2197
                     /locus_tag="HV254_RS25480"
                     /old_locus_tag="HV254_25485"
     CDS             2012..2197
                     /locus_tag="HV254_RS25480"
                     /old_locus_tag="HV254_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2245..2332)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          476..1004
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 aaatgtaatg aatgcgaacg gattcaagaa attcgagcat gacagtcctt acggccggtt
       61 cggtttcaga caaaatctgc cggtatgcat ccagcatcat ggctccggca tcccctccgg
      121 cacgccgtag ccagaccgaa acaacggaca caagcaggtg tcgctcatca tcactaagag
      181 tcatcagggc tccggaagaa aaaccaaaca tggcttacct actgttaacg gcaacgcaga
      241 agaaaggttt acagggtagc gggcagggat gaaaaaaaaa aaattgcagt caggccggaa
      301 cggctgctga accggtacag gcgggttccg ggcgcagcgc cggaccagtc acggagcact
      361 agcggagtgt atactggctt aagatgtagc atggcagtaa cacatagcat taatgccaca
      421 taagacaggc gcagaaagtg aaagaaaacg cgtcaggaga aaatgtgata cagaatatat
      481 tccgcttcct cgctcactga ctcgctccgc tcggtcgttc ggctgcggcg agcgggacgg
      541 gcttacggat ggatcagaaa tttcctgggg gatgccagga agatacttaa cgaagaaagc
      601 ataaggccgc gccaaagccg tttttccata ggctccgccc ccctgacaag catcacagaa
      661 tctgacgctc aaatcagtgg tggcgaaacc cgatgcggct taaagatacc aggcgtttcc
      721 ccctggtggc tccctcgtgc tcccccgcat tcctgccttt cggtttaccg gtgtcactcc
      781 gctgttatgg ccgcggtttc tcattccacg cctgacactc agttccggga aggcagttcg
      841 ctccaggatg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
      901 taactatcaa cttgagtcca acccggaaag acacgactaa tcgccattgg caacggccat
      961 tggcaaacag gatgtgcgag agatgtctca agagttcttg aagtggcggc cttagtgagg
     1021 ctacactgaa aggacaaatt tggtgacatg tctcgcacaa gactgttacc acggttaggg
     1081 gatctgccag gattacctaa ccttcgaaaa accacctgcc agggtggttt tttcgtttac
     1141 agggcaagag attacgcgcg gaaaaaaagg atctcaagaa gatcctttat aaattagaca
     1201 tccaacctca caaagaggaa tacttaaatc atcttggtat atgtatcggc cggattacat
     1261 ctcatataag cctcctcccc gcctgacacc acactttgac catagcacct cgcgcctaac
     1321 tcgatataca gcccaccatc aagcctcttt tcacataaaa aatgacctat agttagatgg
     1381 ttagtgacca aaactgatat ggataaaagg agctggcttt gaaaaagatt tttgtttcag
     1441 tgttcgcagc ggcagttgcg ctttcagccc tcaccggctg cacgcgcaca agctatgcca
     1501 tacacaccaa tgacggtcgc actattgtca gcgacggcaa acctaaagaa tcagactcag
     1561 gcctgcttgg ctatacagac gcgaatggtg tcaaacagca gatcaataaa accgacgtta
     1621 aagaagtctc cgaaattccg cactgagcat cttctccgaa aaaggccgct aatgtggcct
     1681 ttttctttac ctgtggttct ccgccaaaat cccagcaaat tgcatcacca aagctaaaag
     1741 ctttcagggt tgtctaaaaa gcacaagaca taagaggaag tgcggtattt tataatcaag
     1801 cccccaagaa tttaccataa catccgttgc ccgcaccgcc tgagacgcgt tcagcgcgtt
     1861 cctgacgaaa ccatgacaaa aaccacaaca aaccaccccg gaacccgtca gaaacgcgcc
     1921 tgttaaattt taacggcatg catgactatg caccagaatg acgccatgct cttttcacgc
     1981 aaaaatcatc accagacggg gaaaatcacc agtgaccaga caggaatccg ccgccctcaa
     2041 tatggccaaa tttatccgcg cacagacact tctcctcctt gagcggctcg agcagatgga
     2101 tctggatgag gctgccggct gctgtgagca cctgcacgat caggccgaag cgctttacgc
     2161 catgctgaac gcacagatag gcgaggaaaa tgcgtgaaaa tcggtgaacg ggtgcgcaat
     2221 tcagtgcgcg gccgtgaggc gatggcgggg tgtcggggcg cagccctgac cagggtattt
     2281 gtgatgccgg cgcgtgcgcg gtattacaaa tgcacatcct gtcccggaac ggacaccggg
     2341 aaacagcaaa aaaaaccggg cggcacgccc ggaactcaat caagttagat tagattactc
     2401 tcactcgtcc ataacagcat catggaacga cgaccaccgt ccgtgacggc cgcctcgttt
     2461 aagtatggac agaaatacag aaaatgctca ggacg
//
