LOCUS       NZ_CP056679             4858 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00642 plasmid pRHBSTW-00642_4,
            complete sequence.
ACCESSION   NZ_CP056679
VERSION     NZ_CP056679.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148671
            Assembly: GCF_013734275.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00642
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.032019356373x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734275.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,011
            Genes (coding)                    :: 4,900
            CDSs (with protein)               :: 4,900
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 34 of 111
            Pseudo Genes (incomplete)         :: 85 of 111
            Pseudo Genes (internal stop)      :: 19 of 111
            Pseudo Genes (multiple problems)  :: 24 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056679.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00642"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00642_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4738..4858,1..74))
                     /locus_tag="HV254_RS25765"
     CDS             complement(join(4738..4858,1..74))
                     /locus_tag="HV254_RS25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_115667475.1"
                     /translation="MLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENAMLNERVS
                     DLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(<46..126)
                     /locus_tag="HV254_RS25770"
                     /pseudo=""
     CDS             complement(<46..126)
                     /locus_tag="HV254_RS25770"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(102..593)
                     /locus_tag="HV254_RS25775"
     CDS             complement(102..593)
                     /locus_tag="HV254_RS25775"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<628..1332)
                     /locus_tag="HV254_RS25845"
                     /pseudo=""
     CDS             complement(<628..1332)
                     /locus_tag="HV254_RS25845"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1271..1594)
                     /locus_tag="HV254_RS25450"
                     /old_locus_tag="HV254_25455"
     CDS             complement(1271..1594)
                     /locus_tag="HV254_RS25450"
                     /old_locus_tag="HV254_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2577..3005)
                     /locus_tag="HV254_RS25455"
                     /old_locus_tag="HV254_25460"
     CDS             complement(2577..3005)
                     /locus_tag="HV254_RS25455"
                     /old_locus_tag="HV254_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     gene            3290..3718
                     /locus_tag="HV254_RS25460"
                     /old_locus_tag="HV254_25465"
     CDS             3290..3718
                     /locus_tag="HV254_RS25460"
                     /old_locus_tag="HV254_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3775..4266)
                     /locus_tag="HV254_RS25465"
                     /old_locus_tag="HV254_25470"
     CDS             complement(3775..4266)
                     /locus_tag="HV254_RS25465"
                     /old_locus_tag="HV254_25470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(4643..>4804)
                     /locus_tag="HV254_RS25785"
                     /pseudo=""
     CDS             complement(4643..>4804)
                     /locus_tag="HV254_RS25785"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          complement(1708..1881)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.857"
                     /coverage="100.0"
                     /evalue="2.61e-71"
     Typing          1805..2350
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..1281)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.27"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccgcgaagg cgctctccca ttcgtccagc ctttgcgagt agtcctgctg tagctgctct
       61 aatgcgctca gcaactgctt ttccagctct gtcattttcc gctactcctg tttcaggcgg
      121 atccatgtcg tcccgtcgta ggggatggct tcggtttgca tccccggcgg caggataaga
      181 aacctccggc cgtcgctgct ctcctgatag cgcactcccc acgtccgggc ggtcattttc
      241 gccagggtat cttcctgctg acgaagatgc gtgtaattgt cggttatctg ctgcccctgc
      301 caccacagaa tgctcccgct cgtgccgatc agcagcgctg acaccatcag gatggtgagc
      361 catgtccgcc cggcagtccg cagtacgctc cgccggtttt tctccatcgc ctcgctcata
      421 ctctgctcgt gggcgaggat ggcgtcgctg attctcctcg cgcttgcgct cagttccgct
      481 ctgacagact gctcgtgctc gctgaatgcg gctttcagca tctcgccggt actctgctgc
      541 tgcgctttcg attgctgctc taagtccttc gccagcgtta aaagactgct catagatggc
      601 tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatac tgcttttcgt
      661 ggtgcgcacc acctcaaaac ctgcaccttc aagagtgtca gtgacatcct gacggctttt
      721 cagctccccg gacgaagcaa gggtcagcag tccccgggta atggcttctg cggcctcctg
      781 cttcgttttc ggcagcccgg acggcgtgac cagcgcccgg cggttttccg gcgcgttcgg
      841 gtcgtgcagc cccagtctgc cgttcacgat agtctgccag gcatcgatgc gcggccggtc
      901 ggcgcggtcg tagtaaggct ggagccgcct gccggtcagc agctcggtat tggggatcag
      961 aaagttcagc tccagacgcc ccttgtccgc gtgctccacc cacagaatgc tgtactgatc
     1021 tttatcgagt ccgggcatca gaacccgctc gaagctggcc atcagttttt cccgctggcc
     1081 gggaggcaga tcggcctccg caaacgacag aacgccggag gtgtatttct taacgtaagg
     1141 cgacgcgtca atcagctccc ggacttcttc cggcttcccc tgcaggacgg tggcgccttc
     1201 acgctgccgg tctttaccca gcagatagtc caccggcccg gcgccaccgc cgcggccgcg
     1261 cggatgaaat ttaacgatca tcgtccgttc ctttctcaag aacggcgtgc cgcagccgct
     1321 caaggccggc atcgatggcc atcagcgccg ccacaatctg taccctgtca tggccggtgc
     1381 cgccgccggc gttaacacgg cgggctatct ggttcaggtt attgcccatg ccggcaagct
     1441 ggcgcagcag cgccggcgag atcgagggga gctttccgga acgtgccggc ttttcgtcca
     1501 ggcagatctg ccgcatccac gccgccagct gcctgccgtc acagcgctca agcagccgcc
     1561 ggtgctcgtc ctccgtcacc cacatggtga gcatcttgct ccgtttctct gacaaaccgg
     1621 cctcctgata acgccgggac gggcgggaaa gcctttcccg gtcggcgcgg ggttggacaa
     1681 gccgcaggcg cgtcaggctt ttggcgggtt tcggggcgca gccctgaacc agtcacgtag
     1741 cgctagcgga gtgtatactg acttattata ttggcacggg tgcgggaatc agtgaagtgc
     1801 ttcatgtggc aggagaaaaa aggcggcacc ggcgcgtcag cagaatatgc gatacaggat
     1861 atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1921 gtgggcttac gaatggggcg gaaattcctg gtagatgcca ggaagaaact taacagggaa
     1981 acgataaggc cgcggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     2041 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg acttaaagat accaggcgtt
     2101 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     2161 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggaaggcag
     2221 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     2281 ccggtaacta tcaacttgag tccaatccgg aaaaacacga caaagcgcca ctggcagcag
     2341 ccactggtaa caggtttaga ggaggagagt cttgaagttt cgggtgcgat aaggctacac
     2401 tgaaaggaca gttttggtga ctgtgctcct ccaagccagt taccgcggtt caaaagagcg
     2461 tcaactcttg cgaaccttcg aaaaagccac cttgccgggt ggttttttcg ttttcaccgc
     2521 aagagattac gacgagggtc aacgaatctc aagggatcat tcatccgaac aagagatcat
     2581 tcgaaatttt gggggagttg catagggtca tgatacccaa gctctaacag ttttatatgg
     2641 ttcggcgcta gacgttggaa gtggatgacc caatcagaga ctttgtactt cccgtgaaat
     2701 gtatcgatga aaacgggatc gcctatatgg gcatgccaca atttattagc cctggcatgt
     2761 tcttcaatag cttctttgtt ttcgtaactg tcaggcacgt tccaagatgg cgataccttg
     2821 ccaatccaag ccggatggcc ttttaaggca tcgccaaaaa gattgtactt ctcgaaatgt
     2881 tcgatgaagt catcgatttt atcgagtgtt ttttgagaaa agcgggatgt gtaataaaga
     2941 ttgaatgtct cagagaaaga gacaacattc aattgtggat tttgcgtgct tgatccgctg
     3001 cccatgctct tctctgctcc ctacttagac cagatggcat tgtaacagat ctctcattcg
     3061 ctctggaaat caacagagac aaggatacac caaatatttc agtatctttt acaagatcat
     3121 gatgttgttt ggttttcata tcactaactc cgtatttaat gcagatagaa atgtttttct
     3181 gttctcagat tagcctttta tagttgtgta ataatggcgc tggtgtcacc ctattcatat
     3241 gaatacttat attatatata tctcaaaaaa aatatttaca ggataatcaa tgagttacgc
     3301 aatagaaaaa aaatggaagg ctggaggtct gtcattagga tggactgcag tacctactgt
     3361 gttgtttttt atacaaaatg agaagaaatt gagccctgta gcattcaata ccttgctgaa
     3421 tctcatagta cactggtgga atctacagga gtggccgcac ccctcaatgg agagcctggc
     3481 tatacggatg ggggtgtcag taaggacggt gcaaagggct attaatgacc tagaaaaagc
     3541 caatctctta gataaaaaac cgacatcaaa aagcgacaac cgatatggtg gccgcaacat
     3601 atacgacctt acaaagcttg tagattattt agacataatt ggtccttctg tgtccgagca
     3661 agtaagaaag ccgcgacata aaaagccaat atatacagta aggaaaaaag ccgattagtt
     3721 atgctggtta catgcaggaa cagcccgcat ttggaggccg ggcttatcgt taaattaggg
     3781 cttaatcgta agaccagaac gttgaatgta ttcatcaacg cgatcgccaa tgtttacaat
     3841 tttggcgaaa ttcacatcag ccgatgacac gtttagatcc gttgcggatg caggcatagg
     3901 caaggttgca cgatgaccat cgacggaaac caaggttttt ctatcaacaa acgaagaacc
     3961 atagtgaacg gtgtactcga ctgaacacgc attactatca ggatgacgaa cagcccaagg
     4021 ctcattaaac gagttgtagt cgtcgtaatc agctctctta atgtgcagtg ctaaatcatt
     4081 tttataagta aacgcccctg tctcatcatc cacaatccaa tcattttgac tcgattgcgc
     4141 aagaattcgt gtaacttcat cagcattcat aaatagctcc atcaggcagt gtgtagataa
     4201 aaagcaaaca acatcaccta catgggtatt atcagaacct ttatcaacgg acggttgatt
     4261 taccataaca tctgttgccc gcaccgctga aacggggttg gctgcgtttc cggtgccaga
     4321 cgcgagaaag tggcgacaga cataacgaaa aaacaccaga aacgcagccg ctgaatatta
     4381 cactggtgca tagtcatgca gccagatgca tggatgttaa agtcacacta aaatccgcgc
     4441 caatgcctta aaaaccgaca aatgcttctc cagccagtcc cacagctccg ccgccagata
     4501 caggcagtta agcaatgatt caataagatt taacagcttc atttcccgct cctgaataag
     4561 tcgtgacctt tttgatcacg aacctattta gcgggagaaa taagagcccc tcttcgtgcg
     4621 ggttgtcagc tgcctggatg gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc
     4681 gctccagctc cagtgttttc tgatactcat tctccctctg ccggagctcg atggcattca
     4741 gttcccggac aagcggcgca gctgctcgct taaactcagc acctgcgtgc tcaagtccga
     4801 cacgcgctcg ttcagcatcg cgttctcccg ttgcataaga ccacacatgc tccgccat
//
