LOCUS       NZ_CP056667             4803 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00668 plasmid pRHBSTW-00668_5,
            complete sequence.
ACCESSION   NZ_CP056667
VERSION     NZ_CP056667.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148676
            Assembly: GCF_013739595.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00668
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.7085084211158x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013739595.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,899
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 51 of 79
            Pseudo Genes (internal stop)      :: 19 of 79
            Pseudo Genes (multiple problems)  :: 12 of 79
            Pseudo Genes (short protein)      :: 1 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056667.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00668"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00668_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4728..4803,1..116))
                     /locus_tag="HV259_RS24915"
                     /old_locus_tag="HV259_24920"
     CDS             complement(join(4728..4803,1..116))
                     /locus_tag="HV259_RS24915"
                     /old_locus_tag="HV259_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1454..2023
                     /locus_tag="HV259_RS24920"
                     /old_locus_tag="HV259_24925"
     CDS             1454..2023
                     /locus_tag="HV259_RS24920"
                     /old_locus_tag="HV259_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310049.1"
                     /translation="MAKLPLPLPIASPPTVSNSGSERAKKSGRRTLSAKRKTLLEELWG
                     DELEQLKVWNRHEHDGYTTVPRTLTYISRILDYLGGTGLPLSQTYLALWCRVFDEGFVE
                     IRDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTVIKE
                     LYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     gene            2027..2572
                     /locus_tag="HV259_RS24925"
                     /old_locus_tag="HV259_24930"
     CDS             2027..2572
                     /locus_tag="HV259_RS24925"
                     /old_locus_tag="HV259_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310050.1"
                     /translation="MASYFPTLDISGEEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEAFYHPAGEQIIYDHSGNGRTFCPIRYRLSLALPEIINRLCDPAEKVYQTSAK
                     RNWAYSITIDDPEGPYHLFFEIRRASRDEKHLQDLNMVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     gene            3276..3485
                     /locus_tag="HV259_RS24930"
                     /old_locus_tag="HV259_24935"
     CDS             3276..3485
                     /locus_tag="HV259_RS24930"
                     /old_locus_tag="HV259_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(4327..4728)
                     /locus_tag="HV259_RS24935"
                     /old_locus_tag="HV259_24940"
     CDS             complement(4327..4728)
                     /locus_tag="HV259_RS24935"
                     /old_locus_tag="HV259_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          165..635
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.889"
                     /coverage="91.0"
                     /evalue="4.61e-163"
ORIGIN
        1 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
       61 gactgattct ggatgaattt tgccatattc agcgctgttt tctgttgttt cgtcataaga
      121 ggcctcacag cgtcagtgct cagtgtaggg atgttctctt ttccgcttcc tcgctcactc
      181 agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt acaaaagctc
      241 gagaaaaccg agcaatcgcg tgatggggaa aggccgcggc gttgccgttt ttccataggc
      301 tccgcccccc tgacaagcat cacagaatct gacgctcaaa tcagtggtgg cgaaacccga
      361 caggactata aagataccag gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc
      421 ctgccattcg gtttgccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
      481 cagacactca gttccgagaa ggcagttcgc tccaagctgg actgtctgca cgaacccccc
      541 gttcagtccg accgctgcgc cttaaccggt aactatcatc ttgagtccaa cccggaaaga
      601 cacgacaaag cgccactggc agcagccatt ggtaagagtc aatgtgcgag agattttaga
      661 gagtacttga agtgatggcc ggactacagc tacactgaaa gaacagtttt ggtgactcgt
      721 ctcgcacaag acagttacct ggttaagcag ttccccaact aacttaacct tcgatcaaac
      781 cacctcccca ggtggttttt tcgttttcag agcaagagat tacgacgata caaatgggat
      841 ctcaagaaga tccttaacta attgcagctc gcttcactat cacattcaac tcccgtcacc
      901 gtcgattaaa accatataca actacggtca ggtgattccg ctcactggag cgaaattacc
      961 cgttaagctc aaaacgctta acgtaggata attccgtttt ccaagctacc cccatcaaca
     1021 tccacacata gctctcagac gcgctcaggc aagagccaag tcatttcctg ccccaaacct
     1081 caccataacc ataaaaacca ccgaaaatcg aacacagggc actatgagca ggcatatcga
     1141 tttgcattaa atggctgccg taaaattatt accttgcccc cttgcatccc cattcagcac
     1201 cgcgagcaca ttcagcacat tgagcacaat taagcacagc gagcacagaa acctgtcata
     1261 actcgttgaa tatcagtcac catatgctgt atttattaca actcacagat tgagttgtaa
     1321 taaatacata ctcacaagat acctatacgt tgaaaggcaa catcacaacc acacgcaaaa
     1381 cacaacataa ctcattgttt taaaagaaaa aacacttaaa ataaccgtat gttaaatgat
     1441 tttggagaat ataatggcga agttaccact gccactaccg atagcgtccc ccccaacagt
     1501 aagcaatagt ggctcagaaa gagcgaagaa gtcaggaaga agaactcttt cagcaaaaag
     1561 gaaaaccttg ttagaagaac tatggggaga cgaacttgaa caactcaagg tatggaatcg
     1621 ccatgagcat gacgggtata caactgtgcc aagaacgctg acatatatca gtcgaattct
     1681 cgattatctc ggaggaacgg gactgccatt gtcccaaaca tatctggctc tttggtgcag
     1741 ggtatttgat gagggctttg tggaaataag agataaagat ggatttgctt atgaagctgg
     1801 ttttagtgga cagcgagcag taaccacatg gacagggagg atgagaaaac taagagattt
     1861 aggtttcata actacaaaac ctggcacatc aggtgagttt caatatgtga ttttgcttaa
     1921 cccactgact gtcattaagg agttatacga aggtaaggaa aaagatgaaa gatacaatgc
     1981 attagtgggc cgtatgcagg aggttggtgc taaatgggag taattcatgg ctagctattt
     2041 cccgacgtta gacatatctg gagaagagtt cgatttcact caccttgagc cattcattat
     2101 gcacgtaaat agcgatatgg ctaaaaaggt attaaaagta cacgttaggt tcacaaatca
     2161 ttgttttaca aaaggttatg aagcttttta tcacccagca ggagaacaga taatttacga
     2221 ccatagcggg aatgggagaa cgttttgccc aattagatat agattatctt tggcattacc
     2281 tgagattatc aaccgtctat gcgatccagc agaaaaggtg tatcaaacat cagcaaagag
     2341 aaactgggcc tactcgatta cgattgatga tccagagggg ccatatcact tattttttga
     2401 aataagacgc gcctcgcgtg atgagaaaca cctacaagat ttgaacatgg ttatcgagag
     2461 tgcctatcct gaagatcgag ggtacgggcc tccgtcctta ctagggcgaa ttggctttgt
     2521 agggttatgc ggtagggtat tttgtgggaa gcctctggcg actaaaagat gaaaaaaaag
     2581 cacctgaaag gtgccttttt tctgtttctt tggtaatcgt tccggacacc actagctttt
     2641 gcttaggttc gccttgcgcg aacacaggaa caagatttgt cctgctgggt agcgctcaat
     2701 ctcatccggt cagtcgctac gatgtaaaca atataacaaa accaataacg caattcagca
     2761 ccaccggttc aatgcaatgc tttgattcat atggagcacc atgagtacag aaaccataag
     2821 taaccatctg aatttaaatg gcaagatgca ttttataaaa caactcaact tctgagttgt
     2881 tttataaaat gcatctgttc ttatgattca ccatgatttc actacaatag aaaaaaacat
     2941 agatagttgt tttcatttaa aaaataaatc ccctcctgtt ttcatcacct tgatgcattg
     3001 gcaatcgtcg cacggcagct ttcactgata ccctcagcct gacggcagcg gtcggtgtaa
     3061 ttgtcgccta ataattgtgc gaaccctcga ggctattcat cctgccccta aatcagagtc
     3121 tcacacctgg ttttgatgcg ctcaattttt gttctatttt tttgaatgat atccgcaaat
     3181 tgtttcgatt ttaatcagtt catggtcgtc ctatcattta tcacatcagg gcaacaacgc
     3241 cccatacaga acaaaatgac ttaaggaata cgaccatgaa atccatcaaa actttcgttg
     3301 cagttgccgc tctatccatg atgtctttcg gtgcctttgc tcagagcgtc agcgctaccg
     3361 cctccaccct ggatcgtgct gaagcaaaaa ttgccgcaca ggccgctgaa caaggtgcgt
     3421 catacaaaat caccagcgct cagttcaata accgtgtcca catgacggct gaactgacca
     3481 aataagatgg tcgcagaggg agctgccgaa caggtcgccg ggaggcggcc cgtttcgtcc
     3541 cttagcagga aaaccttcag gccccttcgg ggcttttttg catctgtcat ttaacataaa
     3601 ggcatgattg tacgcaccgc taaaacggcc tcagggcgtt tccgacggga aaccggtaaa
     3661 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     3721 catgagtatg cacgcaagtg catagcgcga aaattcacgc cattcagcca gggtaagtcc
     3781 tgtttccggc gttcttccta tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     3841 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acactcagga atggggttcg
     3901 tcgacggccg gcaatgacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     3961 cgattcgact cgtgacttga ctctagagtc gccaaggtca aaaaaacacc aaaatgacga
     4021 ttcgactcta gagtcaagtc gtcataagtt actgatagtc ttaagtgagt cgtcgatgac
     4081 tctatgacga gtcgtcggca agtcgtacac gaatcgccga cgacttggat tcaccttaca
     4141 gaaagtaata ctgcgtaatt taataaccca caactaactg atattaatga gttatcttca
     4201 aaattttaat tttgcgtggg gtgtgggtat ttttagtggt gaggagcgtt cagagatctg
     4261 acgatttttg ggaaggttca ggaactcccc ggaatgaccg gagaggaaaa ctgaagcatg
     4321 acggggtcag gattttttcc agaatttcca ccatggcgcc ggggattcag ttaattttgc
     4381 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga
     4441 caaaagtaag gtgttcaggt gttcaatctc ctgtttcaga tgacgaatca cctgttcact
     4501 ctggtcagca gaggctgtat cacgctgttc agagacctgt ccagaatggt gttcaccctt
     4561 cggttgctca aagggaccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt
     4621 acgttcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
     4681 actcttaccc gtcagtcgtg cagcctcact aatagacact ctggccatca gttaccatcc
     4741 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
     4801 ttc
//
