LOCUS       NZ_CP056666             4858 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00668 plasmid pRHBSTW-00668_4,
            complete sequence.
ACCESSION   NZ_CP056666
VERSION     NZ_CP056666.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148676
            Assembly: GCF_013739595.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00668
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.7085084211158x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013739595.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:50:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,899
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 51 of 79
            Pseudo Genes (internal stop)      :: 19 of 79
            Pseudo Genes (multiple problems)  :: 12 of 79
            Pseudo Genes (short protein)      :: 1 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056666.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00668"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00668_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4821..4858,1..391)
                     /locus_tag="HV259_RS24880"
                     /old_locus_tag="HV259_24885"
     CDS             join(4821..4858,1..391)
                     /locus_tag="HV259_RS24880"
                     /old_locus_tag="HV259_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(448..939)
                     /locus_tag="HV259_RS24885"
                     /old_locus_tag="HV259_24890"
     CDS             complement(448..939)
                     /locus_tag="HV259_RS24885"
                     /old_locus_tag="HV259_24890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(1411..1626)
                     /locus_tag="HV259_RS24890"
                     /old_locus_tag="HV259_24895"
     CDS             complement(1411..1626)
                     /locus_tag="HV259_RS24890"
                     /old_locus_tag="HV259_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1633..2124)
                     /locus_tag="HV259_RS24895"
                     /old_locus_tag="HV259_24900"
     CDS             complement(1633..2124)
                     /locus_tag="HV259_RS24895"
                     /old_locus_tag="HV259_24900"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2159..2863)
                     /locus_tag="HV259_RS25310"
                     /old_locus_tag="HV259_24905"
                     /pseudo=""
     CDS             complement(<2159..2863)
                     /locus_tag="HV259_RS25310"
                     /old_locus_tag="HV259_24905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2802..3125)
                     /locus_tag="HV259_RS24905"
                     /old_locus_tag="HV259_24910"
     CDS             complement(2802..3125)
                     /locus_tag="HV259_RS24905"
                     /old_locus_tag="HV259_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4108..4536)
                     /locus_tag="HV259_RS24910"
                     /old_locus_tag="HV259_24915"
     CDS             complement(4108..4536)
                     /locus_tag="HV259_RS24910"
                     /old_locus_tag="HV259_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          complement(3239..3412)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          3336..3881
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1320..2812)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctgtcatta ggatggactg cagtacctac tgtgttgttt tttatacaaa atgagaagaa
       61 attgagccct gtagcattca ataccttgct gaatctcata gtacactggt ggaatctaca
      121 ggagtggccg cacccctcaa tggagagcct ggctatacgg atgggggtgt cagtaaggac
      181 ggtgcaaagg gctattaatg acctagaaaa agccaatctc ttagataaaa aaccgacatc
      241 aaaaagcgac aaccgatatg gtggccgcaa catatacgac cttacaaagc ttgtagatta
      301 tttagacata attggtcctt ctgtgtccga gcaagtaaga aagccgcgac ataaaaagcc
      361 aatatataca gtaaggaaaa aagccgatta gttatgctgg ttacatgcag gaacagcccg
      421 catttggagg ccgggcttat cgttaaatta gggcttaatc gtaagaccag aacgttgaat
      481 gtattcatca acgcgatcgc caatgtttac aattttggcg aaattcacat cagccgatga
      541 cacgtttaga tccgttgcgg atgcaggcat aggcaaggtt gcacgatgac catcgacgga
      601 aaccaaggtt tttctatcaa caaacgaaga accatagtga acggtgtact cgactgaaca
      661 cgcattacta tcaggatgac gaacagccca aggctcatta aacgagttgt agtcgtcgta
      721 atcagctctc ttaatgtgca gtgctaaatc atttttataa gtaaacgccc ctgtctcatc
      781 atccacaatc caatcatttt gactcgattg cgcaagaatt cgtgtaactt catcagcatt
      841 cataaatagc tccatcaggc agtgtgtaga taaaaagcaa acaacatcac ctacatgggt
      901 attatcagaa cctttatcaa cggacggttg atttaccata acatctgttg cccgcaccgc
      961 tgaaacgggg ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac agacataacg
     1021 aaaaaacacc agaaacgcag ccgctgaata ttacactggt gcatagtcat gcagccagat
     1081 gcatggatgt taaagtcaca ctaaaatccg cgccaatgcc ttaaaaaccg acaaatgctt
     1141 ctccagccag tcccacagct ccgccgccag atacaggcag ttaagcaatg attcaataag
     1201 atttaacagc ttcatttccc gctcctgaat aagtcgtgac ctttttgatc acgaacctat
     1261 ttagcgggag aaataagagc ccctcttcgt gcgggttgtc agctgcctgg atggtttaaa
     1321 gtgtcggccc gcggtaggtt ttctgcggct ggcgctccag ctccagtgtt ttctgatact
     1381 cattctccct ctgccggagc tcgatggcat tcagttcccg gacaagcggc gcagctgctc
     1441 gcttaaactc agcacctgcg tgctcaagtc cgacacgcgc tcgttcagca tcgcgttctc
     1501 ccgttgcata agaccacaca tgctccgcca ttccgcgaag gcgctctccc attcgtccag
     1561 cctttgcgag tagtcctgct gtagctgctc taatgcgctc agcaactgct tttccagctc
     1621 tgtcattttc cgctactcct gtttcaggcg gatccatgtc gtcccgtcgt aggggatggc
     1681 ttcggtttgc atccccggcg gcaggataag aaacctccgg ccgtcgctgc tctcctgata
     1741 gcgcactccc cacgtccggg cggtcatttt cgccagggta tcttcctgct gacgaagatg
     1801 cgtgtaattg tcggttatct gctgcccctg ccaccacaga atgctcccgc tcgtgccgat
     1861 cagcagcgct gacaccatca ggatggtgag ccatgtccgc ccggcagtcc gcagtacgct
     1921 ccgccggttt ttctccatcg cctcgctcat actctgctcg tgggcgagga tggcgtcgct
     1981 gattctcctc gcgcttgcgc tcagttccgc tctgacagac tgctcgtgct cgctgaatgc
     2041 ggctttcagc atctcgccgg tactctgctg ctgcgctttc gattgctgct ctaagtcctt
     2101 cgccagcgtt aaaagactgc tcatagatgg ctcccttcag tcggatgttt cgccccccgt
     2161 ccgggtcggc aatgctgata ctgcttttcg tggtgcgcac cacctcaaaa cctgcacctt
     2221 caagagtgtc agtgacatcc tgacggcttt tcagctcccc ggacgaagca agggtcagca
     2281 gtccccgggt aatggcttct gcggcctcct gcttcgtttt cggcagcccg gacggcgtga
     2341 ccagcgcccg gcggttttcc ggcgcgttcg ggtcgtgcag ccccagtctg ccgttcacga
     2401 tagtctgcca ggcatcgatg cgcggccggt cggcgcggtc gtagtaaggc tggagccgcc
     2461 tgccggtcag cagctcggta ttggggatca gaaagttcag ctccagacgc cccttgtccg
     2521 cgtgctccac ccacagaatg ctgtactgat ctttatcgag tccgggcatc agaacccgct
     2581 cgaagctggc catcagtttt tcccgctggc cgggaggcag atcggcctcc gcaaacgaca
     2641 gaacgccgga ggtgtatttc ttaacgtaag gcgacgcgtc aatcagctcc cggacttctt
     2701 ccggcttccc ctgcaggacg gtggcgcctt cacgctgccg gtctttaccc agcagatagt
     2761 ccaccggccc ggcgccaccg ccgcggccgc gcggatgaaa tttaacgatc atcgtccgtt
     2821 cctttctcaa gaacggcgtg ccgcagccgc tcaaggccgg catcgatggc catcagcgcc
     2881 gccacaatct gtaccctgtc atggccggtg ccgccgccgg cgttaacacg gcgggctatc
     2941 tggttcaggt tattgcccat gccggcaagc tggcgcagca gcgccggcga gatcgagggg
     3001 agctttccgg aacgtgccgg cttttcgtcc aggcagatct gccgcatcca cgccgccagc
     3061 tgcctgccgt cacagcgctc aagcagccgc cggtgctcgt cctccgtcac ccacatggtg
     3121 agcatcttgc tccgtttctc tgacaaaccg gcctcctgat aacgccggga cgggcgggaa
     3181 agcctttccc ggtcggcgcg gggttggaca agccgcaggc gcgtcaggct tttggcgggt
     3241 ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggcttattat
     3301 attggcacgg gtgcgggaat cagtgaagtg cttcatgtgg caggagaaaa aaggcggcac
     3361 cggcgcgtca gcagaatatg cgatacagga tatattccgc ttcctcgctc actgactcgc
     3421 tgcgctcggt cgttcggctg cggcgagcgg tgtgggctta cgaatggggc ggaaattcct
     3481 ggtagatgcc aggaagaaac ttaacaggga aacgataagg ccgcggcgaa gccgtttttc
     3541 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     3601 aacccgacag gacttaaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc
     3661 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     3721 ccacgcctga cactcagttc cgggaaggca gttcgctcca agctggactg tatgcacgaa
     3781 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcaacttga gtccaatccg
     3841 gaaaaacacg acaaagcgcc actggcagca gccactggta acaggtttag aggaggagag
     3901 tcttgaagtt tcgggtgcga taaggctaca ctgaaaggac agttttggtg actgtgctcc
     3961 tccaagccag ttaccgcggt tcaaaagagc gtcaactctt gcgaaccttc gaaaaagcca
     4021 ccttgccggg tggttttttc gttttcaccg caagagatta cgacgagggt caacgaatct
     4081 caagggatca ttcatccgaa caagagatca ttcgaaattt tgggggagtt gcatagggtc
     4141 atgataccca agctctaaca gttttatatg gttcggcgct agacgttgga agtggatgac
     4201 ccaatcagag actttgtact tcccgtgaaa tgtatcgatg aaaacgggat cgcctatatg
     4261 ggcatgccac aatttattag ccctggcatg ttcttcaata gcttctttgt tttcgtaact
     4321 gtcaggcacg ttccaagatg gcgatacctt gccaatccaa gccggatggc cttttaaggc
     4381 atcgccaaaa agattgtact tctcgaaatg ttcgatgaag tcatcgattt tatcgagtgt
     4441 tttttgagaa aagcgggatg tgtaataaag attgaatgtc tcagagaaag agacaacatt
     4501 caattgtgga ttttgcgtgc ttgatccgct gcccatgctc ttctctgctc cctacttaga
     4561 ccagatggca ttgtaacaga tctctcattc gctctggaaa tcaacagaga caaggataca
     4621 ccaaatattt cagtatcttt tacaagatca tgatgttgtt tggttttcat atcactaact
     4681 ccgtatttaa tgcagataga aatgtttttc tgttctcaga ttagcctttt atagttgtgt
     4741 aataatggcg ctggtgtcac cctattcata tgaatactta tattatatat atctcaaaaa
     4801 aaatatttac aggataatca atgagttacg caatagaaaa aaaatggaag gctggagg
//
