LOCUS       NZ_CP056590            12351 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00355 plasmid pRHBSTW-00355_5,
            complete sequence.
ACCESSION   NZ_CP056590
VERSION     NZ_CP056590.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148590
            Assembly: GCF_013738135.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00355
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.3410414469136x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013738135.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:43:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,375
            Genes (coding)                    :: 5,195
            CDSs (with protein)               :: 5,195
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 54 of 180
            Pseudo Genes (incomplete)         :: 121 of 180
            Pseudo Genes (internal stop)      :: 37 of 180
            Pseudo Genes (multiple problems)  :: 26 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056590.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00355"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00355_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(12316..12351,1..552))
                     /locus_tag="HV173_RS27170"
                     /old_locus_tag="HV173_27175"
     CDS             complement(join(12316..12351,1..552))
                     /locus_tag="HV173_RS27170"
                     /old_locus_tag="HV173_27175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_061301009.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            813..1472
                     /locus_tag="HV173_RS27175"
                     /old_locus_tag="HV173_27180"
     CDS             813..1472
                     /locus_tag="HV173_RS27175"
                     /old_locus_tag="HV173_27180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548402.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            1655..2044
                     /locus_tag="HV173_RS27180"
                     /old_locus_tag="HV173_27185"
     CDS             1655..2044
                     /locus_tag="HV173_RS27180"
                     /old_locus_tag="HV173_27185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            2037..2399
                     /locus_tag="HV173_RS27185"
                     /old_locus_tag="HV173_27190"
     CDS             2037..2399
                     /locus_tag="HV173_RS27185"
                     /old_locus_tag="HV173_27190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301020.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPFLHHHPGDRPAT
                     EKRAIARFKDSLAAGGQTLAFVRSDASLYALGLRFRRERTRELVASDPDVAAAGEALEQ
                     RANGVNLSYTFGGGDE"
                     /name="hypothetical protein"
     gene            2392..2577
                     /locus_tag="HV173_RS27190"
                     /old_locus_tag="HV173_27195"
     CDS             2392..2577
                     /locus_tag="HV173_RS27190"
                     /old_locus_tag="HV173_27195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151322523.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRRELPRSTGR"
                     /name="hypothetical protein"
     gene            complement(2597..3238)
                     /locus_tag="HV173_RS27195"
                     /old_locus_tag="HV173_27200"
     CDS             complement(2597..3238)
                     /locus_tag="HV173_RS27195"
                     /old_locus_tag="HV173_27200"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_061548403.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQC
                     LIDQCMRYDLDGNPTVAVTPEEAEQAKTRLTEIREFRRKARQEKDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(3235..4524)
                     /locus_tag="HV173_RS27200"
                     /old_locus_tag="HV173_27205"
     CDS             complement(3235..4524)
                     /locus_tag="HV173_RS27200"
                     /old_locus_tag="HV173_27205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181213195.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            4880..5089
                     /locus_tag="HV173_RS27205"
                     /old_locus_tag="HV173_27210"
     CDS             4880..5089
                     /locus_tag="HV173_RS27205"
                     /old_locus_tag="HV173_27210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            5076..6248
                     /locus_tag="HV173_RS27210"
                     /old_locus_tag="HV173_27215"
     CDS             5076..6248
                     /locus_tag="HV173_RS27210"
                     /old_locus_tag="HV173_27215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548407.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRHAVRHARLERG"
                     /name="hypothetical protein"
     gene            6479..6925
                     /locus_tag="HV173_RS27215"
                     /old_locus_tag="HV173_27220"
     CDS             6479..6925
                     /locus_tag="HV173_RS27215"
                     /old_locus_tag="HV173_27220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548408.1"
                     /translation="MKSKTELRAAATRRALEVIASEMGGWSLDGFAHGSLPNFSPLPSQ
                     QTQEGSVVLARSPFECSWAGTAVFTAQASRFVNADPASAHYEWACGVEREAVATALMVE
                     AFNVTGCVEYAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAA"
                     /name="hypothetical protein"
     gene            complement(7293..7784)
                     /locus_tag="HV173_RS27220"
                     /old_locus_tag="HV173_27225"
     CDS             complement(7293..7784)
                     /locus_tag="HV173_RS27220"
                     /old_locus_tag="HV173_27225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548409.1"
                     /translation="MAIGDDAIRDAFYIFTQQAAEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDLPTSPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAARRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCRAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(7854..8333)
                     /locus_tag="HV173_RS27225"
                     /old_locus_tag="HV173_27230"
     CDS             complement(7854..8333)
                     /locus_tag="HV173_RS27225"
                     /old_locus_tag="HV173_27230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548410.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKNARQFAQQTARQSARQIVPRGADPRFQAVSD
                     EADQLIVSMTGENATLPAIASALNAAGYRTATGQEWNTSRVRDYRHKLRESGLLC"
                     /name="hypothetical protein"
     gene            8879..10402
                     /locus_tag="HV173_RS27230"
                     /old_locus_tag="HV173_27235"
     CDS             8879..10402
                     /locus_tag="HV173_RS27230"
                     /old_locus_tag="HV173_27235"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_061548411.1"
                     /translation="MPNHDDNSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGSPTRDGFRIQAAEKRSVNFGNEYPLRYVPPLITDAGSGRWDVLNHADNLRWL
                     LYRNGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNELLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            complement(11089..11556)
                     /locus_tag="HV173_RS27235"
                     /old_locus_tag="HV173_27240"
     CDS             complement(11089..11556)
                     /locus_tag="HV173_RS27235"
                     /old_locus_tag="HV173_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_061548400.1"
                     /translation="MKKYTPEQKAQALKLLEQDGATAATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRQQS"
                     /name="transposase"
     gene            11812..12030
                     /locus_tag="HV173_RS27240"
                     /old_locus_tag="HV173_27245"
     CDS             11812..12030
                     /locus_tag="HV173_RS27240"
                     /old_locus_tag="HV173_27245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015766.1"
                     /translation="MKEFHVFKDRILAAAKESSPFWVTFEYPEDCFGYDERFVFESVSA
                     LKSMRILGLEIHNAADRRFSARFLPVE"
                     /name="hypothetical protein"
ORIGIN
        1 gcggtaaatc gcgctccggc ttaggtttag ctgtttggct atggtggcga tcggcactcc
       61 catggcgcgg cgcatgacaa tctccccggc ttccttcgct ccgatcttgg tcgggcgtcc
      121 gaacttctgg cctcgactct ttgcggcctc tatccctgct gcctgccgtt cgaggatgag
      181 cttgcgttcc atctgcgcga agctggccat tacgccgagt accgcttgtc cgacggcgct
      241 gctcaagtcg acgccctcgc ggatgctgac caaggtgatc ttccgcttct ccagctcctc
      301 gaccagctcg agggtgtccc gggcgttcct gccgatgcgg tcgatcgcgt aaatgaccag
      361 ggtgtcgccc tcctgcagca gctggttcag gaggatgccg agggcggggc gctcgcgtgg
      421 tggtaccgtc ccgctgatcc cgtcgtcggc aaggatggag gcggttggga accgggcgat
      481 gatggcgtcg cgctggctgt tgctggtttg ttcgtcggtt gatacgcgaa cgtaaccgta
      541 tgttttgctc acttttttct ctcctatgac cgatacgtct ctttcgacac aacatgccac
      601 aactgtatcg aaagtctatc gctaaagtgt ctttttcgtc gctcctgcgg gtgtcctgaa
      661 atgtaggggt ttagcgacgg gctctcgggc ggctttccgc gctgggcgag agccctgcgc
      721 cctctgcgtg ggtgtgctcg gttggtcgtg acggcatgct gtctgagcgg cgtttcttgc
      781 cgctttgctg ataacttgct gagaacctaa ccatgaccga aattaagaaa gagagcgccg
      841 acggcgcttg gctacgctct acccttgccg aattggcggg tgatgcgttc cctgtgtatg
      901 acctcccgtc cctgcaggta gacacgaact cctccattca gctctcggcg ctcgccgatc
      961 gtcaggctgc gcgtgaaatg cgacaggccg cttccgacgt ggaagcgcga cggctggacg
     1021 ccgctcgagt ggtggaaggg ctcaagaccg aggccgaacg gctgcggggt ttgatcgccg
     1081 acggcaaggc tgcgctgcgt gctggcgagc cggtgagccc cgacgcgggt gttgcgtcgt
     1141 tcctgctgcc ggacatcgag gccgagctgg ttgttgctga ggctgctgag gccgacgtgg
     1201 cccgggagcg tgacaccctg ctgcaggatg ccgatcgccg tgacgcggcg gctgcgctgg
     1261 cgctgttcaa ctgggcgcac tccgttcgcg tgcagcgtat cgagctgctt ctcaagctgg
     1321 cgatggacga ggcgacgacg ctcgctgaaa ccgacggcgg gcgcggcctg taccgtaccg
     1381 tgatcgcgcc tgaccgccgt cttaaccaaa ttttcgccac gcagggcgct attgaaatcc
     1441 tgcgccgtaa ccgtggcatg gagggtgtgt aatgtctgct ttcaaactcc tgtgtgaccg
     1501 gcttcgcaat gaggcgacgc tggcaaaatc tgacggggcc ggcgctttca aaaagcaatc
     1561 tctggatccc gacctgaagg gcggcaagaa gggcaaaaag cccgctgggg agctggagga
     1621 ggacgaggcg atcgcgtctc tccaccgtaa gcgcatgaac gacgttaaag gttgcaagac
     1681 gctgaccaaa tccaccggcg ctgatgactt tggcctccct ccgctgtcgt cggtggtcga
     1741 gacagtggtg gagacaggcg gcgcagcgca gacgttcgcg ccgatcgggg ctccggtggc
     1801 gtctccgacc gaagcggttc accgtgcgcg tgcgcagtcc atccaggacg cggcgttcct
     1861 gaacgagatg aaagcggcgg gtgtggcacc ggctgacctg gtgttacttg acggcgtgtt
     1921 gacgtcctct cgtgggatgc cagacgcggc gcgtgctccg gcgtttgccg aggtgcgccg
     1981 tcaggttcag gcgggcggca tgacgctgtc accggctgcg cgtggtattg actggaatgc
     2041 gtaagctgcc gcgtcacctg cagggcgagg tgttgatgcc gctgtccagc aggatcacgc
     2101 gctcgcaacg gctggagctg atctggctgt tgccgttcct gcatcaccat ccgggcgatc
     2161 gtcctgccac cgagaagcgg gctattgccc ggtttaaaga ctcgctcgct gcgggtggcc
     2221 agacgctggc tttcgtccgt tcggatgcgt cgctgtatgc gctggggctg cggttccggc
     2281 gtgagcgcac ccgggagctg gtcgcttctg atcctgatgt tgctgcggct ggcgaggcgc
     2341 tggagcagcg tgcaaacggt gtgaacctgt cctatacgtt cgggggtggc gatgagtgaa
     2401 tttgctgtct cgcgcctggt cattcctgag gtggtgatcc ccgatggggt cattgctgcc
     2461 cgacgggcaa ggggacaggc tcaacgtgtc gcagctgctc gccgtgctgc agagcgtcgt
     2521 gctgcgccta cgtggcgctg ggtgagacgg gaactgcccc ggtcgacagg gcggtgaaac
     2581 ggtgagacgg ggcgcgttat gcgccctttt ttttgtcctt ctcctggcgt gccttgcgtc
     2641 ggaattcccg gatctctgtc aggcgggttt tggcctgttc ggcctcctct ggcgtgacgg
     2701 cgacggtggg gttgccgtcg aggtcgtagc gcatgcactg gtctatgagg cactgctggt
     2761 acgagctggt acccgtgaca atgcccagga tgcgacggac ggctttgcgg tctgcctctg
     2821 gcggcatgtc cagctctgcg cctcgggtca ggatctgctc gatgatgccc tttgccatgg
     2881 ggcgacggtc aactccccgg gtcagcaagt caggccagtg ctctttcagg aatgctttgg
     2941 tcacccggtt ggccagcgcc tttttggact tgttggcgat cgcgatgtcc tgctggcggc
     3001 ggtcgttctc ctcctgtctg cggatcccct cggcgacctg ctcgggcgtc tttttcggct
     3061 ttgccgctgt gggcttctgt gcgggcttct gcggggcttt cgcttttggc tgtaccttcg
     3121 ccttgctggt agtccgttcg gctggctgcg gctcagattt cgcggccttt tccggcggga
     3181 cgtagaccgt gcggttgcgt cgcttgatgg tcaggatcgg acgggtggtt tcggtcataa
     3241 atctctctct ggtcggttgg tccggatgtg gtcggcaagg gcgatcaggg tggcggcgtc
     3301 gccgcggtcc cgatcgatgc gccggatgat gtcgttgatg tagtcccagc gcccgacggc
     3361 ggcaaggacg gaaagggcga ggcggttgaa ccggctctct ggtacccggt cgagggtcag
     3421 ggggatgcgc ttgcgggggc gtcctggacc gcgccctgtc cctggtcttt tgagcagctg
     3481 cggctgctcc ctctgcggca aaataacggg gatcttcgcc aggctgcgcg tctcgttctg
     3541 cgcgaactcg ggcaccgcga tgtgctcgtc tgtccatgtc ctgcctccgg gcctggctga
     3601 tgcgctggag tcgcaggacg ggcaggagac gacgatctcc cgtcgcggct tgcccttgac
     3661 cggtgccttg ctgatcacca ggggcgcggc tttgcaatca gcgcagaccg acgatgtcca
     3721 gaccgcgttg tctggcaggg tggcgttccg gtactggtgc gcggcgtcat gcggtgtctg
     3781 gaagctgacc gtctgaaacg tgggggcgac gctgaggggt gccgcccatg gctggcacct
     3841 gggacaagca agctccacct ctccggcgtg cccgtcgcca ccgttccagc gcaggggctc
     3901 gcggtgttcg gggcagtagt acacgaaacc gtcgcggcgt gtttgcccga ccgggcctgg
     3961 tcggcgatcg tggaaccgtg cgcccgggtt ggctcgcatg gcgcggacga gcgcctcgcc
     4021 ctggtggatg ccgtaccaga cctgccagcc atcgccgatg ccgatcagga acggcgagac
     4081 gaacggtggg gtgcggttga ctctcccgcg tgcgttggct ccgatggtgg cttcgcgttc
     4141 gcggttccag caggagacgc acaggctcga accgggctta ttgggggcga tcaggcggtt
     4201 gtctttccgg ctgcaacggg ggcagagctt acgggtgtct gccggggtgg tcagtggtgc
     4261 gtcagggggc tgttcggtac cgacccaaag cgcgaggcca gtggggcacc ggtggcactc
     4321 gaggtggggg ttttcaatcg tgcgatcgag gtggctctgc tcataaatct gtcggcatgc
     4381 ctcgacccgg agggagcacc cgccggggca ggtgacgaac aaaacgccgg ggagctgctc
     4441 ggggaatttt tcagtcgcgc ttacgcgttc gagtgctgcc gggttcggct gcgtcgattc
     4501 gttcgtgtcg ttatcgtcgt tcactgctcc ccctgcgtcg aaatggtcgc cagaccgcgc
     4561 cagcactggc tatgagtctt ttttagtatc tcacaaaaaa cgccactttg gcgttaattg
     4621 tgttacgctt gtcttactga ttcctgacga gcagttggga aacatgaaac cggatcgccc
     4681 ggtgccgctt tccctcctcc ccaacgaggc ggggttgtaa acgaggatgc gcgcttcgtc
     4741 cttggcaggt gcgagtaagc gagaagggct gctgtcctgc cgctatgcgg taagtcctca
     4801 agggcggctt tatcgataat cgtcactccc gccccccgat gcccgttaag ctgagggggg
     4861 cacctatctg gtactcccaa tgaaaaaacg aatctatacc gctgacctcg ctgcgctgct
     4921 gaacgtgtcg ctgcgcacgc tctcgcggct aagttcgcgg gggatcgtgc cgactccggc
     4981 agggcgctcc ctccgtggcg ggcattactg gctggctggc aatcccgaga tcaaacgctt
     5041 cgttgccgac cagcgtcgtc cggggagggc tgctgatggc gttcgttgat ttcgacacta
     5101 cccatctgaa agaggatccg ggcctgttct ggcgtgctgt atcggctgcg ggtgctccgg
     5161 ctgatcgaga cgaatgggtg cgtctctgcc tggctcttgg ttgctgggcg tctctctgcg
     5221 gcgaggacgg gctggcagct atcgaggcct ggtccactcg acatgggcga ggcgagaatc
     5281 gccatcgcca gcagtacagg tcgtttttaa aaggcgacct ggatacattc gccgctcagg
     5341 ctttcctcaa tttctccggc gtcgaatggc gcaacggtcg cgcttctgtc cagtccgacg
     5401 tcgatcgcgc tgaacggctg cggttgtccc gtgaggccac agcgaaggca aaggctgaac
     5461 gcgacaagaa gggtgatgct gctgcgcgtg ctgcggtcaa gctgctggcg aagggagggg
     5521 atcctgcgtc taccccatac tggcgcaaaa agaccgctct ggcggctgcg ggtggcgtcc
     5581 agctgcccgc cctcagcaca gatcttggtg ttggcgggat gtttcgatcg ttcgatccgc
     5641 gtgcttactg gtctgattgc ctcctgattc cggtttacaa gttcgacgcc gcccgtcagc
     5701 tgtatgttga cggcctggag gccatctgcg ggcgctgctc ggctgagggt tcccagctca
     5761 ctggctcgaa gttctctccg gcgggtaccc gcaagcaggg cggcttctac ccgatgccgg
     5821 ggttcatgga cgtgctcgac ggctccccgc acgcgcccta ctcgcctctg ttcgtctgtg
     5881 aggggttcgc tacggcgttc gccctgcacc acctgagccg tggcaaggtg gcggtctgcg
     5941 ctgcgttctc gatcgccggg ttcggcagct gcgtggcgtc gctgcgtcgg ttctggcctg
     6001 atgttgaggt gatgcttgta ccggacgcgg gagaggcgta ccggctggcg cataaagcgg
     6061 cctatggcga cgatcgcgag ccggtcccga caattccagg ggcgtcggtg gtgtcgttcg
     6121 acggcctgaa ccgcttcgag ccccgcgata acctcgactg gttcgacgtg ctggttgagg
     6181 agggcgagga tcgggcgcgt gagctgctcc ggcacgcggt gcgtcatgcc cggcttgagc
     6241 ggggctgagt ggcgtgtccg aatggtgcgc ctttcgggac acccttcctg ctaccgccgc
     6301 tccggctttt gcgaccgact ttcgagacac tatcgccgct tttttgcaga gacggattaa
     6361 agcgacgcac cgccgctgcg gtcgtgacgc tatcctgcag ctggtttggt ttccagcagc
     6421 acactcaagc ccggcaccgt tttggtgtcg ggcttttttt cgcccgtaag gagggcacat
     6481 gaaatcaaag actgaattac gcgctgccgc gacccgtcgc gcccttgagg tgatcgcttc
     6541 tgagatgggg ggctggtcgc tcgacgggtt tgctcatgga tcgctgccga acttctcccc
     6601 gctgcctagc cagcagacgc aggagggctc ggtggtgctc gctcgatcgc ccttcgagtg
     6661 ttcgtgggct ggcactgctg tgttcaccgc tcaggcgagc cggttcgtga atgccgatcc
     6721 ggcgtctgct cactacgagt gggcgtgcgg cgtcgaaagg gaggctgtgg ccaccgcgct
     6781 gatggttgaa gcgttcaacg tgaccggctg cgtggagtac gccgggttgc ctcctgtgga
     6841 cggcatggtg ctgctgacca tggacgaggc cgacgtggag ctgatcaggg cggcgatgct
     6901 gccttggtta gatgcggcgg cgtaggccgt cgctcctctg cgtcagatca ggccacgctg
     6961 acgcagaggc tggatacgct agatggctac ggctaaagcc acaggtgacg cgggctcatt
     7021 gagttcagga gagcattatc attccacttt tggttgaatc ttagctgtaa tccgtataga
     7081 attattttac tgcaagagcg ttctacagat acgtaaatta ttctgtagtc agcagtaagg
     7141 agctaaaact cctgaccaat agcgaatttg atagacagca gaaaaccgcc gatgctgcta
     7201 gatccgaaag gaaccaatga agggaaatat ttaggcgtta tggttcattc tcattaaatt
     7261 gaggagaact gcttaaatgg gcgtccttcc tatcaccctc gctgtatagc tctacacttg
     7321 gcctccgttg catggtatgc agttacgggg gccaaattgt tttcagagac atcaactatc
     7381 gccattgcga aaaaaccagc cccagcgacg ttaatacgac gagcagcatt cagcacaata
     7441 gtctgctcag aataatccct gttaacgtag acgttactct gcactgaaaa tgcagtagcc
     7501 aaaatttccg cacactcctg ccaacttaat ttatccttcg agttacgtgc agatagcaca
     7561 tgcctaaaca tagcccaagt aattggtatt gctggcgacg taggcaaatc ccagccgttt
     7621 ctctgacaaa caaccttcat ctgattgaat tgttttttag tcatcaaata agtgaaacaa
     7681 ggagtctccc agacttcttg agggagcccc ttatcatcca tttccgcagc ttgttgcgtg
     7741 aagatgtaga aagcatcccg gattgcatca tctccgatag ccactgactt tccttataaa
     7801 taatttaagg ctgcatttta tcatatttaa ttacctcact taggactgct aactcagcac
     7861 aatagaccac tctcccgcag cttatggcgg tagtcccgga cgcggctggt gttccactcc
     7921 tgccccgtcg ctgtccggta cccggctgcg ttcagcgccg acgcgatggc gggcagggtg
     7981 gcgttctccc cggtcatgct gacgatcagc tggtcggcct catcgctgac ggcctggaag
     8041 cgtggatcgg ctccccgggg gactatctgt cgtgcgcttt gtcgtgcggt ttgttgtgca
     8101 aactgtcgtg catttttccc gcctccggcc agcgccctga cctctgaccg cagcgcctcg
     8161 atggcagtca aaagtttggt gtccatccct tcaggcttcg tgccgagtcc ggcgacgagc
     8221 gcctctatga cggcatctgt gcgggttact ttggttttct ccgctcgctg ctcgatgcgc
     8281 tcgatcaggc tgacgggcag tcgaacggtc agggttttcg ttttctctcg catgggctag
     8341 catctccgtg tcgtgctatt tgtcgtgcta tttgtcgtgc tgtttgtctt gcggagtgta
     8401 atgcgttttg tcgtgcgcgt cgaatttgac gtagtgcaat tgtcgtgcgc tgaggggcga
     8461 cggcgttgcg tcgccggggt gcctgtggac agtttctgtg gacaagtatt ttgcggcgga
     8521 gctggcccgt ggataagtgg tagaggctac gcggctgtaa ggctggagct ggatcgcgtt
     8581 tcgcgtggtt ggatcgttgt gtgctggtac aggggggaat gtaacggtat gatgacgcgg
     8641 ctgaaaaaga aaacccgtcc tggcagacgg gtcattttct ttaaagctgt tgttgttaaa
     8701 actttaagga gctttcgcgg cgggaaccgt ggaagacacc gattctaaca acaggttggg
     8761 tggcaatcca gcttgaattg tacgcgaact ttccagcgcg tgcaaagggt gttttccccg
     8821 taaagtcccg ccgttgtagg gctcctcaag attcgatgtc aattttgagg atgtaaaatt
     8881 gcctaatcat gatgataatt ctggcgctgc tgtagccact gtaacgcaga tcgccgccgc
     8941 caaggccaaa gctgccgcca agaccgagcg ccctgcgttc atgggtggtt ctctgcctcc
     9001 tgtcccgttc aatgagtggg atgggctcac cggtagcccg acccgcgacg gtttccgcat
     9061 tcaggcggct gaaaagcgat cggtaaattt cggcaatgag tatccactgc ggtatgtccc
     9121 gccgctgatc actgatgctg gctctgggcg ctgggacgtc ctcaatcacg ccgacaatct
     9181 ccgctggttg ctctaccgca atggtgcccg gatggagctt aacctcatga ccttcaagaa
     9241 tgagctctca tgggctgata gcggctctct ggtcgaaccg gacgagcatg gtgcccgcgc
     9301 ctggatactg aacaaaagcg ccgaggttgg cctgaaacgt caggctgttg acgatttcat
     9361 gttctacgtt gcccgttccg ctccctacca ccctgttgcc cgcctcctcg gttcccgcga
     9421 gtgggacggt tctcctcgcg tcgatgccgt gctggactgc gtacctgtcg cacctggtaa
     9481 ggaggcgctg cgctcggctc tgctgcgtgg actgctgatt gcggcgatca ccgctgttga
     9541 ggaaggggct gtgtcgaaca agtacgttcc ttgcctctac tccgaggcta acaactggtt
     9601 taagacggcg ttctttcgcg ctctgttcga cctgctgcct ggtgcgttcg ggcagggtgt
     9661 gtctatcaac ccttcgagta aggacagcgt gcgccctgcg gtcatgcgct ggtttaacga
     9721 gctgggtgaa gtgtcgggca tgatgaagaa aaacgacccc gatgaaatca aggcgttcgt
     9781 ggagcgcaac gtggatgagt ggcgtcctga aaacgagaag gcggtgatcg ccaaaaagcg
     9841 ccagatggtg atgggtgcca cggtgaacaa gcgcgactac ctgaaggacg agacggcggc
     9901 gtcccgcttc ccgaccatta ccctcgtcgc ccctatcgac attgctcgcg ttaacgagct
     9961 gctcgggtgg gagtgggata acgtcaaagg ctgcgctgtt cgggcggatg aggatcagct
    10021 gatccagttc tggctcgagg ttcgtcacct gtaccgctct ggcgtcggtt tcaacctgga
    10081 cgacgagacc tttgctgcgg cgaaagctga gacggctcaa ttcgtcgatc gcggctcgta
    10141 ctatgaaccg gtgcgcgaca tcctcctgtt gcctggcaat gcgtccactg gccagtaccc
    10201 gtgggataag cctggctatg acggcaggtt taccgcgacg gacgtgacgg cggcgctgcg
    10261 tgccccatca agccattctg gtcgcgtcgg tagggcgttg aagcgcctag ctgatgaggg
    10321 gatcgttgat cgcaggaacc accggggtac ttttgtttac tggaaattgc cagacgaacc
    10381 aaccggggaa ctcccgctct gaaaatgacc cgcttcggcg ggttttttta tgcctctgcc
    10441 aatgttgggg tacccgtgtg ggggttccgt tttgtgtggg ggtatggggt gatcatgtgg
    10501 gggttcggtg tgggggtaac gtgtgggggt aactggtgat gttggggtaa gatgtggggg
    10561 taaactcgca aagaaaatct accgccacat actcaaaatc cctttcatgg cgctgcatgt
    10621 ggctttcgtg ttggggtatg tgggggtaca ttcttttatt tatctactat gtaaaaagag
    10681 gggtaataag tagcccccac accccctgta cgcccatatg ttacccctac accctcatac
    10741 ccccacacgc tcaaaaattg cgcagttggc gtgccgcagc tgcacccctg aaggggatgc
    10801 actttgcttg ccttgtgagg tggggtgatc gggggcgcag aggacgaagc tgtcgcttcg
    10861 ctgggtggtc gtctggtcgt tctgcgtgag ggcttgccct cgcgccctct gcggcttgtg
    10921 tggaatgcgt agctgcagct gcgcgtttgg ttcgtccggg catcaaaacg ggacttctcg
    10981 cgcacgcgag cggtaacaat tgcgtaacgt gcggatccgc gtattccggg ggctcccggg
    11041 ttttctccgg cggtaaaacg gcggtaaaag gcggctttcc cgccgttttc agctctgctg
    11101 gcggttcatg ccctgccgca tgatgtcggc ctcggtgccc ggcttgatct cgtagatgag
    11161 gcgctcgcgt tcctgcgcct taagcaggga ggtcatgagg ccgctgatcg ctttgaccat
    11221 ggtggcgtcc ttctccagca tggcctgtag cgatgcgctg cgtagggctt gcaggtcgct
    11281 cgcgtggcgc tggagtagct cgtactggcg ctgcacaaag tggtttttga ggcgtcggac
    11341 tgcggcctgt ggggtggcct cgacggctcg ctgcgcgcgt tcgcgcatct cctcgatgct
    11401 gaggacgttg cttggtgcgt cggcgcgggc tttcgcccaa tctctgacgg tgcggggtgg
    11461 gatccccatg gtgcgtgcga cggtggcggc ggttgcgccg tcctgctcca gtagcttgag
    11521 cgcctgggct ttctgttcgg gggtgtattt cttcatggtt cctgctccgg ttggtttcct
    11581 ccggctattt gagcgtcacg cgctgagaat ggcggcaact tttggggggt gtacggcgct
    11641 gtctcgtgct gggtggtatg tcggtgcggt tttggggctg gggcgcttag gattgctctg
    11701 gtgcgttctg gtgtgtggtg ccttatgtac gtcccgggcc gactccatta tgagatttcg
    11761 ttttcctgta aaatgctcgt gagcaccttc gctcttaatg agggtatggc aatgaaagag
    11821 ttccatgtct tcaaagatcg cattcttgcg gcagcaaaag agtcctcccc gttttgggtg
    11881 acatttgagt acccggaaga ctgttttggg tatgacgagc gttttgtctt tgaatccgta
    11941 tctgctttga agagtatgcg gatactagga ctcgagatac acaacgcggc tgatcgccgt
    12001 tttagtgccc gctttctgcc tgtagagtga gtgtgcttac ggtgtaagtg gttcgttatg
    12061 gggtaacgct tacaccgtaa ggagttcgtt atgccgccat ctcacggcgt agacgcttca
    12121 tggctctgcg ccaggtctgc gggttgcggt tggtgactgc gcggcgtgcc agctggcgtg
    12181 cgatgctctg tggtgatacg atcatggtgt tacctcattt gggtgggtta acatgcccgg
    12241 gttggctctc acacttcccc gggcttgggt tgcggcggtg gctttagcga gtaccaccgc
    12301 cgcgctctct ctctcttact ccggcgcgac cgcgtcgact ttctcaagat g
//
