LOCUS       NZ_CP056555             4426 bp    DNA     circular CON 30-APR-2024
DEFINITION  Enterobacter sp. RHBSTW-00422 plasmid pRHBSTW-00422_4, complete
            sequence.
ACCESSION   NZ_CP056555
VERSION     NZ_CP056555.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148606
            Assembly: GCF_013749735.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. RHBSTW-00422
  ORGANISM  Enterobacter sp. RHBSTW-00422
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00422
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.2302188371287x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013749735.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 03:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 39 of 109
            Pseudo Genes (incomplete)         :: 84 of 109
            Pseudo Genes (internal stop)      :: 24 of 109
            Pseudo Genes (multiple problems)  :: 28 of 109
            Pseudo Genes (short protein)      :: 1 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterobacter sp. RHBSTW-00422"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00422"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742646"
                     /plasmid="pRHBSTW-00422_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4367..4426,1..285))
                     /locus_tag="HV189_RS25630"
                     /old_locus_tag="HV189_25630"
     CDS             complement(join(4367..4426,1..285))
                     /locus_tag="HV189_RS25630"
                     /old_locus_tag="HV189_25630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015387218.1"
                     /translation="MSEKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     TGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            912..1022
                     /locus_tag="HV189_RS25970"
     CDS             912..1022
                     /locus_tag="HV189_RS25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063858821.1"
                     /translation="MHEPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(1278..1739)
                     /locus_tag="HV189_RS25640"
                     /old_locus_tag="HV189_25640"
     CDS             complement(1278..1739)
                     /locus_tag="HV189_RS25640"
                     /old_locus_tag="HV189_25640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138058728.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYHDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            2073..2288
                     /locus_tag="HV189_RS25645"
                     /old_locus_tag="HV189_25645"
     CDS             2073..2288
                     /locus_tag="HV189_RS25645"
                     /old_locus_tag="HV189_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            complement(2976..3191)
                     /locus_tag="HV189_RS25650"
                     /old_locus_tag="HV189_25650"
     CDS             complement(2976..3191)
                     /locus_tag="HV189_RS25650"
                     /old_locus_tag="HV189_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3198..3689)
                     /locus_tag="HV189_RS25655"
                     /old_locus_tag="HV189_25655"
     CDS             complement(3198..3689)
                     /locus_tag="HV189_RS25655"
                     /old_locus_tag="HV189_25655"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(378..551)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          475..1079
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..264)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.318"
                     /coverage="82.0"
                     /evalue="3.08e-51"
     Typing          complement(2885..4377)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgatggcc atcagcgccg ccacaatctg taccctgtca tggccggtgc cgccgccggc
       61 gttaacacgg cgggctatct ggttcaggtt attgcccatg ccggcaagct ggcgcagcag
      121 cgccggcgag attgagggga gctttccggt gcgcgcaggc ttttcgtcca ggcaggtctg
      181 ccgcatccag gccgcgagct gtttaccgtc gcagcgttcc agcagacggc ggtgctcatc
      241 ttcggtcacc cacatcgtga gcattttgct gcgtttctct gacaaaccgg tctcctgata
      301 acgccgggac gggcgggaac ctctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
      361 cgtcaggctt ttggcgggtt tcggggcgca gccctgaacc agtcacgaag cgctagcgga
      421 gtgtatactg gcttactatg ttggcacgga tgagggtgtc agtgaagtgc ttcatgtggc
      481 aggagaaaaa aggcggcacc ggtgcgtcag cagaatatgt ggtacaggat atattccgct
      541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcgga aatggcttac
      601 gagcggggcg gaaatttcct ggtagatgcg aggaagagac ttaacaggga aacgagaagg
      661 ccgcggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      721 gctcaaatca gtggtggcga aacccgacag gacttaaaga taccaggcgt ttccccctgg
      781 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ctccgctgtt
      841 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
      901 agctggactg tatgcacgaa ccccccgttc agtccgacta ccacgcccat tccggtaact
      961 atcaacttga gtccaacccg gaaagacacg acaaaatcgc cattggcggt agccactggt
     1021 aagcaggatg tgcgagagat gtatcgagag ttcttgaagt ggtggccgga ctacggctac
     1081 actggaagga cagatttggt gacacgtctc gcacaagact gttaccacgg ttcagaagtt
     1141 ccccaactta ctgaaccttc gaaaaaccac ctccccaggt ggttttttcg ttttcagagc
     1201 aagagattac gcgcagagca aaacgatctc aagaagatca ttttacaaat tggataatat
     1261 ccctccgccc cctcacacta atataaaatt atcgggcttg catgcccctc accattttcc
     1321 ccatatccac caccaaacaa tagttcgtcg agttctttgc tggcttgatc aatacacgca
     1381 gccacatgaa tagcagaagg gtattcatca tactgaaaat catcgctttt ttcaggagga
     1441 agtcgatcat gatacggatg acaatccaca taataatgac gtattaccat cccagtttta
     1501 tcttcgtact ctgatggctg caacgtactc cctgggaaag cttttgcaac atgtatagca
     1561 gcttgaaagg tatcaaaata tcgcccaaac attggcattt caaagctagg cgactctgtc
     1621 gaaaaatccc ccataacttg cgccaaataa ccattatcta tctgccattg aacatctgat
     1681 cccatagccc actctcccag agttactcct aataaataac cataatatgt atttaccata
     1741 acgcttcact tcgcgaacca gcacttccca cagtaaatcc cttacctagc taaccttggc
     1801 acacgaaaac catcataaag gcaacatatc agcttagggt tatctacctc tcaagctccg
     1861 agaaaaactg ccgataattt tagtgcacta tacaaatatt tttaaaatca atggagtatt
     1921 caatggcacc gacaatctac cgggctaagt gtttggattg taagcatgag tggaccagtc
     1981 gtactggtta tggcacgcct gattattgcc ctaaatgtcg gagtcgtcgg atcacaacaa
     2041 gtccaattgg ataagcatta aggagtggtt aaatgggatt ttgggaaacg gccggtaaaa
     2101 ttgcaaaagg cgcagctaac gctttggatg aaaaagctca tgaactccag gcaaccaaaa
     2161 tgcgcttaga aagcaaaagc agtgaagaac taaagcgcct cattaaatct gatggttttt
     2221 tcggctcagc cagtaacaca gaaaaaagca tggcaatgaa aattctcaga gaacgcggag
     2281 acgtttaagg atagaaaaat ggtaggcacg gtgaatcttc ctcatacatg ccctaactgc
     2341 aagcgaacca ccgctcatac tgcaggtgag ttgcaaactc tattcggatt cagaaccatt
     2401 ccttctggtg caacgaatca aagttggtgc agagaatgtc gtggtaagtc atagcgtcca
     2461 gaagcccgta acccgggctt ttttacgccc acttatttac cataacgtcc cattgcccgc
     2521 accgctgaaa cggggttggc tgcgtttccg gtgccagacg cgagaaagtg gcgacagaca
     2581 tgacgaaaaa acaccagaaa cgcagccgct ggatattaca ctgatgcata gtcatgcagc
     2641 cagatgcatg gcgcgtaaag tcacactaaa atcagcgcga acgccttaaa aaccgacaaa
     2701 tgcttctcca gccagtccca cagctccgcc gccagataca ggcagttaag caatgattca
     2761 ataagattta acagcttcat ttcccgctcc tgaataagtc gtgacctttt tgatcacgaa
     2821 cctatttagc gggagaagta agagcccctc ttcgtgcggg ttgtcagctg cctggatggt
     2881 ttaaagtgtc ggcccgcggt aggttttctg cggctggcgc tccagctcca gtgttttctg
     2941 atactcattc tccctctgcc ggagctcgat ggcattcagt tcccggacaa gcggcgcagc
     3001 tgctcgctta aactcagcac ctgcgtgctc aagtccgaca cgcgctcgtt cagcaccgcg
     3061 ttctcccgtt gcataagccc acacatgctc cgccattccg cgaaggcgct ctcccattcg
     3121 tccagccttt gcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgcttttcc
     3181 agctctgtca ttttccgcta ctcctgtttc aggcggatcc atgtcgtccc gtcgtaaggg
     3241 atggcttcgg tttgcatccc cggcggcagg ataagaaacc tccggccgtc gctgctctcc
     3301 tgatagcgca ctccccacgt ccgggcggtc attttcgcca gggtatcttc ctgctgacga
     3361 agatgcgtgt aattgtcggt tatctgctgc ccctgccacc acagtatgct cccgctcgtg
     3421 ccgatcagca gcgctgacac catcaggatg gtgagccatg tccgcccggc agtccgcagt
     3481 acgctccgcc ggtttttctc catcgcctcg ctcatactct gctcgtgtgc gaggatggcg
     3541 tcgctgattc tcctcgcgct tgcgctcagt tccgctctga cagactgctc gtgctcgctg
     3601 aatgcggctt tcagcatctc gccggtactc tgcttctgcg ctttcgattg ctgctctaag
     3661 tccttcgcca gcgttaaaag actgctcata gatggctccc ttcagtcgga tgtttcgccc
     3721 cccgtccggg tcggcaatgc tgatactgct tttcgtggtg cgcaccacct caaaacctga
     3781 ccgttccagc gcctcagtga cgtcctgacg ggttttcagc tccccggacg aagcaagggt
     3841 cagcagtccg cgcgtgatgg cttctgcggc ctcctgcttc gttttcggca gtccggacgg
     3901 cgtgaccagc gcccggcggt tttccggtgc gttcgggtcg tgcagcccca gtctgccgtt
     3961 cacgatggtc tgccaggcat cgatacgcgg ccggtcggcg cggtcgtagt acggctggag
     4021 ccgcctgccg gtcagcagct cggtattggg gatcagaaag ttcagctcca gacgcccctt
     4081 gtcctcgtgc tccacccaca gaatgctgta ctgatcttta tcaagtccgg gcatcagaac
     4141 ccgctcgaag ctggccatca gtttttcccg ctggccggga ggcagatcgg cctccgcaaa
     4201 cgacaggacg ccggaggtgt atttcttcac gtaaggcgac gcgtcaatca gctcccggac
     4261 ttcttccggc ttcccctgca ggacgctggc accttcacgc tgccggtctt tacccagaag
     4321 atagtccacc ggcccggcgc caccgccgcg accgcgtgga tgaaatttaa cgatcatcgt
     4381 ccgttccttt ctcaagaacg gcgtgccgca gccgctcaag cccggc
//
