LOCUS       NZ_CP056554             5631 bp    DNA     circular CON 30-APR-2024
DEFINITION  Enterobacter sp. RHBSTW-00422 plasmid pRHBSTW-00422_3, complete
            sequence.
ACCESSION   NZ_CP056554
VERSION     NZ_CP056554.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148606
            Assembly: GCF_013749735.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. RHBSTW-00422
  ORGANISM  Enterobacter sp. RHBSTW-00422
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00422
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.2302188371287x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013749735.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 03:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 39 of 109
            Pseudo Genes (incomplete)         :: 84 of 109
            Pseudo Genes (internal stop)      :: 24 of 109
            Pseudo Genes (multiple problems)  :: 28 of 109
            Pseudo Genes (short protein)      :: 1 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056554.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Enterobacter sp. RHBSTW-00422"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00422"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742646"
                     /plasmid="pRHBSTW-00422_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4156..5631,1..285))
                     /locus_tag="HV189_RS25590"
                     /old_locus_tag="HV189_25595"
     CDS             complement(join(4156..5631,1..285))
                     /locus_tag="HV189_RS25590"
                     /old_locus_tag="HV189_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="WP_003030990.1"
                     /translation="MTRDDFNSDTRTVGLARLANSLEETYGSFINMENTNSKGEARREV
                     LITRCIAAHAVRMYGDELDPEVAAASVCDGSDDGGVDAVYVNPSSKKLILVQSKYIKDG
                     NGSVTIKDFGRFKDACAKVVANDLNSFNDRFLEHKESVTTAITSSSYKCICVFIYSGTQ
                     GLSSEVIGEIDFWEKSQNKALLFRDLESRDEYTILFETPNVTDISTSLSNQSTGAIDIS
                     NVILESYGEITEPYGAIYGTIAARVIHDWWKKYKYALFEKNIRSVLGDSTSVNAGIMST
                     IENSPENFWYYNNGITATYEEVDESIANAGASRKIGIFGFKKLNIINGAQTVSSIGAIF
                     DSIPEEKKDKARVMIRFINAKDPVFLGDVTKYNNTQNRVTGRDFTTQRPEQQKIQREIN
                     FVGGYTYKLLRQEDSGIAASNVIDIDDSLNALICASKKPQLIATLKSNRGRFFDTTEGN
                     LYEQVFNPRNPPSGLSVINAVNLYRVCFDVLKDTITNASNSSEEEYSKIPSLLTHGNYV
                     FISILMGKIQPTNTVNDINTYDKNSVSVDTLELAKSIFEYIQSNYPNSYLARFFQNKEK
                     VEQIITHFSA"
                     /name="AIPR family protein"
     gene            complement(272..1174)
                     /locus_tag="HV189_RS25595"
                     /old_locus_tag="HV189_25600"
     CDS             complement(272..1174)
                     /locus_tag="HV189_RS25595"
                     /old_locus_tag="HV189_25600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_003030989.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALETSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNLQFASPLE
                     DKEVMGIAKSISKWTEKNFTASSFKEYVINSHKSDIQSKRGRLGGLKSKGGGRPTINSP
                     WKDMGISKSTYYRNIHDKR"
                     /name="replication initiation protein"
     gene            complement(1366..1650)
                     /gene="nadS"
                     /locus_tag="HV189_RS25600"
                     /old_locus_tag="HV189_25605"
     CDS             complement(1366..1650)
                     /gene="nadS"
                     /locus_tag="HV189_RS25600"
                     /old_locus_tag="HV189_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_003030988.1"
                     /translation="MSFFDELKASLEEAVEIKNGVRAPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(1652..1984)
                     /locus_tag="HV189_RS25605"
                     /old_locus_tag="HV189_25610"
     CDS             complement(1652..1984)
                     /locus_tag="HV189_RS25605"
                     /old_locus_tag="HV189_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_003030987.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKMLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2146..2379)
                     /locus_tag="HV189_RS25610"
                     /old_locus_tag="HV189_25615"
     CDS             complement(2146..2379)
                     /locus_tag="HV189_RS25610"
                     /old_locus_tag="HV189_25615"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023207133.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALT
                     HSDRVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2440..2925)
                     /locus_tag="HV189_RS25960"
     CDS             complement(2440..2925)
                     /locus_tag="HV189_RS25960"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_145981112.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSTAMMWIFLCFALLIGILGGIIWFQGTIITSRLSEMD
                     SYSQQLASLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2972..3394)
                     /locus_tag="HV189_RS25965"
                     /pseudo=""
     CDS             complement(<2972..3394)
                     /locus_tag="HV189_RS25965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3603..3929)
                     /locus_tag="HV189_RS25620"
                     /old_locus_tag="HV189_25625"
     CDS             complement(3603..3929)
                     /locus_tag="HV189_RS25620"
                     /old_locus_tag="HV189_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_003030983.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLIVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2049..3613)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaaataagt tttttagagc ttgggtttac ataaacagca tcaactcccc catcatcgct
       61 accatcgcaa actgatgcag cagcaacttc agggtcgagt tcatcaccat acatcctcac
      121 tgcatgcgca gcaatacatc ttgtgatcag aacctctctt ctagcctcac ctttagagtt
      181 ggtattttcc atattaataa aagaaccata ggtttcttct aggctattag ctaaccttgc
      241 aagtccaacg gttctagtgt cgctattaaa atcatctctt gtcatggata ttcctataat
      301 atgtcgattt actaatcccc atatctttcc acggggagtt tattgttggc cgcccgccac
      361 ctttgctttt taatccgcct aagcggcctc tcttagattg aatatcactc ttatgagagt
      421 taatgacgta ctctttaaat gaagatgccg taaagttttt ttcagtccat tttgatatgc
      481 tttttgcaat gcccattact tctttatcct ctaaaggaga agcaaattgt aggttgtaag
      541 cgctggctct ttcgtaacag gcttgcaacc actggtcgta ctcaggccag ccctgacgga
      601 tggcgcggta tgcccacttg cgagttttgt cgaacaacgt gcagttacga cccaggccgt
      661 agtcggcgac aatctctttg tcgttcgccg cgttcagatc gagaaaatcg gctaaccagt
      721 ccagtgtgta gagttccggc tgccatacgg caattttcca gtgcccgtgg ttcggatttt
      781 tacagattag ccccgaatac cccgcgtcag cctctaattt tttacgtagc gcgttctcca
      841 cggcagcggc gtagcgcagc ggcttcattt ttccgtccgg cgcggtgcgt atcgaggttt
      901 ccaacgcgta cagcaggtgc gcgtgtccgt tgtcggggtt ggtgatggtc agcgtgggcg
      961 cgggggcgtt acggtcgctc cagtcgatgg cggcaccggc gcgatcgacg tcgaatccca
     1021 gccagaacat ggcgtgaggc tgattgaact gaatgtattt cgccagaatg gcgcgttcct
     1081 taccggcaat gcgtacacca aagtgcagat cgtccgaaaa gtacggcttg tgcggtaagc
     1141 gttcgttgaa gagttcgagt gcggcgttat gcaatgcgcc ctgcgcttat ggcgggctgt
     1201 tgtctggcac ggttcatgtg ctaatatcct ttcaaggttt cgacctggcc ctggttatca
     1261 tgcggatccg ccaagattag acatgatgcc ggggctttgt tttttctgtt gccgttaaat
     1321 tacacgcgaa cggcctcgcc tgtcgaatcc ttctgtcaca acggcttagt gcgccgccag
     1381 ctcctggaag aatttcgggt ttgcctggat ggttgccagc acctttgccg ccagtccggt
     1441 cggattgcgg cgtccggttt cccagctctt gatagtgtcg aggctggttc ccagcacttt
     1501 cgccatctcg ctctgtgaaa cgttcagctg tgccctgatg gccttcacgt ctgccagctc
     1561 gtaacgggtg acgcgggcgg gcgctctgac gccgtttttg atttcaacgg cttcttccag
     1621 cgatgctttc agctcgtcaa aaaaactcat gtcacacctc atctttcagt tgttgggtca
     1681 gcatcttcag cgcggctttc tccgcgtccg tcagactgtc tttggtgctt ttcgggtagg
     1741 ccatcaccag ataaataacc tcggcggtgg ccagaaaata aatcaccctc gcgctaccgc
     1801 tttttccctg gttgcccgtc gccattctga cttttcgcag cccgccggtt ttctggatga
     1861 gatcgccttt gtccggcgac tcaatcagcg tcttttgcag ctccctgagc tcttcatccg
     1921 tggcgatctg ttttatctga cgggtaaaca tcggggtctc gataaactca atcgcgtgac
     1981 tcacgactgt ctcctgtttt taatggggta catagtacac caaactctga tttcggaaaa
     2041 gcggtcacat cgagggacca tagtagcggg tcggtttttt ctgtggctcc ggttccggct
     2101 gctgatcgcg cagcttctcc gcgtaggcgc gatcatgctg cctgatcagc gcatcaagcg
     2161 gctgacgttc ctgctcaagc tctcgacctg cgcgcgcaag ctgtccacct gactgctcaa
     2221 gtgctgacac acccggtcgc tgtgcgtcag cgcctgtgag gtagcctcga acatgtgctg
     2281 caaactgctg tagctgtcct gccacgcctg ctgctgttgt tcgtagcttg tctgtaagct
     2341 ctctaaggcg ctcagaagct gtttttccat ctcggtcatg attttggtcg tcctcggtgt
     2401 taaaaacgtg ctgtggcgtt tcagggcgct ctgacggggt tatttcgccg tatatttcac
     2461 cacggtgttg tcggtcgtcg aggtgtaggt ttctacgccg tgcgcctgct tcggcagcac
     2521 caccaggtag gtgttccgac gcgtgtcgtc gctgtaaatc gtcacgcccg cgccgctttt
     2581 cgctttcagg ctcgccagct gctggctgta gctgtccatt tcgctcaggc gcgaggtgat
     2641 gatcgtgccc tgaaaccaga ttatcccgcc caggatgccg atcagcaggg cgaaacagag
     2701 aaatatccac atcatcgccg ttgagacgga ggttcttatc atgcccgtca gcctgccgtt
     2761 ctgctcgcgg atagcgttgc tgatgctctg cgagcttgat tgcagttccg cgctgataga
     2821 cgtctccagc ctcgcgaact cgcgcttcac gctctgctcg gtatcctgcg cctgctgctt
     2881 cgatttctcc tcgaagccct tcgccaaatt taaaatctcg ctcataaatc attcctttca
     2941 gccggatatt tcgaccgccg tccggatcgg caatactgat gctgcttttc gtttcccgcg
     3001 ctacggtcag gccgtagctt tcaagctgcg ccactacgtc ctgccggctg cggatcacgc
     3061 cctgctgcat caggttcatc aggccgacag tgattttctc tgccgcctgc tgtttgtcgc
     3121 ggggaagatc gctggcctgc gtcaacgggc ggcggaaagt cgggtcgttc ggatcgcgca
     3181 accccagccg gtcattggtc agggtctgcc aggcgttgac gcggggacgg tcggcgcggt
     3241 caaagtaagg ttgcaggcgt ttgccgctct gcaattcgat gttcgggatg acaaaattca
     3301 gctcaagccg ccccttgtcg cggtgctcta cccacaggca ggtgtactgg tcgcggtcaa
     3361 ggccggtcag cagcgtatgc tcccattcat ccatcaggcg cgatttctcg gcgtctgcaa
     3421 tatccggctc ctgaaatgac agcacgccgg aggtgtaggc gcgggcaaaa tcgcagccgt
     3481 cgatcagctc gcgtacgtac tcaggattgc cgcgcagaac gctggcatgt tcgcgctgcc
     3541 ggtcttttcc cagcaggtaa tccaccggcc cgctgcccga acccgcaccc cgcgaatgaa
     3601 ttttaacgat cacggttctg ctccagcagg tcgccaagct gacggtcaag gctggtcagt
     3661 acgatgagca gcgaggcacg ttcagaaggc gtcagggaat cagtctggtt caggcggcgg
     3721 gctatctggt tcaggttgtt gccaatcccg ctgacctgtc gcagcagttc aggtgccacg
     3781 tcgggaacac ggcgcttgcg tgaggtgtgt tcacccagac caagctgacg aagccactcc
     3841 gcaaggtgag tccggtcgca gcgatcaagc agtcgctgat gctcggtttc ggtaagccgg
     3901 attttgatct ctttcgttcg cttttccata ggttccgctg agaagaaatt cgcaccactg
     3961 tgagaatttt tcgcagtgga tgccaggggt ttcggggggc ggcgagcccc ctgaaccagt
     4021 cacagacggc acctcataga ggggacgctg tgtgtactgg cttaaagtaa agtgcagtat
     4081 gacagattcc gtgctggctg caaaaaaaag ccctccgcta gagagggctg ttatctgata
     4141 taggcttgtt tagtctcacg ctgaaaagtg agtgatgatc tgctctactt tttctttgtt
     4201 ctggaaaaat ctagccagat aactattagg ataattagac tggatgtatt caaatatgct
     4261 tttagctagt tcaagagtat caacagaaac gctattcttg tcataggtgt ttatgtcatt
     4321 aactgtattt gtgggttgta ttttacccat taaaatactg atgaatacat agttaccatg
     4381 cgtcagcaat gatggaattt tactatactc ttcttctgat gagttagatg cgttagtaat
     4441 tgtatctttt aaaacatcga agcacactct gtacaaattg acagcattga taacagaaag
     4501 accggatggc ggatttcttg ggttgaaaac ttgttcgtaa agattgcctt ctgttgtatc
     4561 aaaaaaacga cctctattag acttcaacgt tgcaatcagt tgaggtttct ttgatgcgca
     4621 aattagtgca tttaatgaat catctatatc gatcacatta gaagcagcta tccctgaatc
     4681 ttcctgtcgc aagagcttat atgtataacc cccaacgaaa tttatttctc tctgtatttt
     4741 ttgttgttca ggtctttgtg tcgtgaaatc tctgccagta actctattct gagtattgtt
     4801 gtacttagtt acgtcaccta aaaacactgg atctttcgca tttataaacc taatcattac
     4861 ccgagctttg tctttttttt cctcgggtat actatcaaat atagctccaa tagagcttac
     4921 agtttgcgcc ccatttatta tatttaactt tttaaatccg aatattccaa ttttacgaga
     4981 tgctccagca ttagctatgc tctcatctac ctcttcatat gtcgcagtaa tcccattgtt
     5041 ataataccaa aaattctccg ggctattttc gatggtagac ataattcctg catttactga
     5101 tgtcgaatca cccaatacac tccttatgtt tttctcaaaa agcgcatatt tgtatttttt
     5161 ccaccaatca tgtatcaccc ttgcagcaat cgttccgtag attgcgccgt aaggttcagt
     5221 gatctcacca tacgactcta atattacatt acttatatct atagctccgg tagattgatt
     5281 actcaagcta gtagatatgt cagtaacatt aggcgtttca aatagtattg tatactcatc
     5341 cctagattct aaatccctaa acaacagagc tttattttgg cttttctccc aaaaatcaat
     5401 ctctccaata acttctgagc ttaacccttg ggtccctgag taaataaaaa cgcatataca
     5461 tttatagcta ctactagtga tagcagtagt cactgattct ttgtgctcta aaaacctatc
     5521 attaaatgaa ttgagatcgt tagctacaac ttttgcacat gcatctttaa agcgtccaaa
     5581 atcttttatt gttacagacc cattcccatc ttttatgtac tttgactgca c
//
