LOCUS       NZ_CP056550             1934 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Citrobacter sp. RHBSTW-00424 plasmid pRHBSTW-00424_5, complete
            sequence.
ACCESSION   NZ_CP056550
VERSION     NZ_CP056550.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148608
            Assembly: GCF_013741155.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00424
  ORGANISM  Citrobacter sp. RHBSTW-00424
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00424
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.4247629843307x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013741155.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:41:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,404
            CDSs (total)                      :: 5,288
            Genes (coding)                    :: 5,114
            CDSs (with protein)               :: 5,114
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 44 of 174
            Pseudo Genes (incomplete)         :: 136 of 174
            Pseudo Genes (internal stop)      :: 28 of 174
            Pseudo Genes (multiple problems)  :: 31 of 174
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056550.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Citrobacter sp. RHBSTW-00424"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00424"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742647"
                     /plasmid="pRHBSTW-00424_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1244..1934,1..26))
                     /locus_tag="HV191_RS26845"
                     /old_locus_tag="HV191_26850"
     CDS             complement(join(1244..1934,1..26))
                     /locus_tag="HV191_RS26845"
                     /old_locus_tag="HV191_26850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_064761962.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTE
                     SKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(283..585)
                     /locus_tag="HV191_RS26850"
                     /old_locus_tag="HV191_26855"
     CDS             complement(283..585)
                     /locus_tag="HV191_RS26850"
                     /old_locus_tag="HV191_26855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          1327..1518
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
ORIGIN
        1 gactgataaa aacgggcttg ccacatcagg gatcgcctcc actggcagac aggacagtgc
       61 cggacacggc agaaatgcgc ctcacgtagc ctaaggcgag tttctccagt ttctttgagg
      121 gtagaccaac caaacctcag taaaccgcca caggaagcca tacgcgcccc gtagcgctca
      181 tattcggctg cgagaaggta aatcccacct acgtcgtcag aaacgctttt gtgagcgtcc
      241 cagggcgcgt ctgaggggga gtaatccgtg aggttctgga gtttagggtt ttgcatgcac
      301 accgtcccat gctgggcgat ggctcatttg acgtgttatc attgccgtat cacctgttgg
      361 ttgtcgcttc gaatcgaatt tctgcaggtg gtcacacccc cgaacgatgg ccgtcgttgc
      421 gggggtcgct tttaaggcga ccactcccaa cagaatactt ccaaaacctt ctacaacgca
      481 aataagcccc gaagccttgg tacgcaaggc ttgatgccat tttttatcgc cagagttatc
      541 ccttaagtat aaatacaagg atcgcgcgcg gatcgaacca gccattcaac gcccacccgc
      601 caccaaaaac tgccacaacg ccaggagcga aacagcaacg ctcaacgcac tatcaacatg
      661 attaagaacc tcaaaaaaca taacaacctc actcgaatgg aattgggtaa atctatttag
      721 tctgtgattt gcgaacccat actttttcgc atcacgacag aaccaactcc attcgttctg
      781 gttgtatccg gtaccagaca attaaaaacc gtcttccagg tggttggcct cacttcgtga
      841 atccaaccac ctcagccaat ttttcaatca gcaatgtcgt caatcactaa atcggaaccc
      901 actggcaagc cagcgggacc ccacgattta gagtttgacg atttcggaaa cggcgagcgc
      961 tggcagattt taatctgact gcgtgaggtg ttggcagggt gtcggggcgc agccctgacg
     1021 aatcgttttt ggcaccgaca tcgtgatggc ggtgtaccaa aaacacatct tgtcccaact
     1081 gagttcggaa accgaactca gtaattgagt gacgtcactt tgcggcgtta gccctatcct
     1141 gcatcggagt agaaccaata ggacaagatg tgtttttgga gtaccgccga caagcggcgt
     1201 cctctcaaaa acgtcgtggt cagggctgcg ccccgacacc ctgtcagtct gattcagctt
     1261 tatcttttga cggtgagcga cggtactttt tggattcggt tttccactca aaagcaatgc
     1321 gtgaaccatc atcatcgcct tcgggaatat catccccgat taccatatcg gcatcagttt
     1381 cctgatcgag tttcagtatg tctttgagtg cgccaccggt agccacaaat cggcgcttat
     1441 gtgtctgctt agtcaattcg aggaaccatt caggatcagc aaccatgtca gccggcttcg
     1501 tggaatattt cagggtttca gccactgcgc cccgtacaag ttccgcagtt gcagaagcca
     1561 atgattctcc gtccttaggc ttgcggggtt ttactgctcg aacatcaaca ttcggatcgt
     1621 aattcacacg caaacactct cgccaaagct ctacccagcg gtcatgttta acgtaatctc
     1681 gcgtgaacat tgagggagga accatcatca gagtgtgaaa gtgcgggtgc gcactaccgt
     1741 cacttccacg tgtaacttcc gtggtgcgga tccagccctg aacaggtcga aactctttgc
     1801 gatccttaag tcgctggaag gccatgttca ttcggttaag tgtctcaccc aattccccga
     1861 tcgcgcagtt tcgcactgtg agggtcagga acatccagcg tgcatctgga taatccgcaa
     1921 caatacgcgg cagg
//
