LOCUS       NZ_CP056538             3398 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00433 plasmid pRHBSTW-00433_9,
            complete sequence.
ACCESSION   NZ_CP056538
VERSION     NZ_CP056538.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148610
            Assembly: GCF_013737695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00433
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.61164450012x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013737695.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 02:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,637
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,309
            CDSs (with protein)               :: 5,309
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 69 of 203
            Pseudo Genes (incomplete)         :: 143 of 203
            Pseudo Genes (internal stop)      :: 32 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056538.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00433"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00433_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3236..3398,1..269)
                     /locus_tag="HV193_RS27915"
                     /old_locus_tag="HV193_27935"
     CDS             join(3236..3398,1..269)
                     /locus_tag="HV193_RS27915"
                     /old_locus_tag="HV193_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660103.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071448170.1"
                     /translation="MKAKTEPLEKKWGERSMLMGWTAIPTSLLFLQAELSISPLGFNLL
                     MNLISHWWETTEHPYPSQESMAKRMGVSKRSIQREITTLVELGLLDKQPNKAGSKKFKG
                     RNTYDLSKLVQVLNEKTPDKISSFKRYKKNGESLDSELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            279..731
                     /locus_tag="HV193_RS27920"
                     /old_locus_tag="HV193_27940"
     CDS             279..731
                     /locus_tag="HV193_RS27920"
                     /old_locus_tag="HV193_27940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001141956.1"
                     /translation="MPTFTQSGTGKFDYWLIDGVKSFSKIPANTLPSITVDMPIRLQVG
                     NGYFGSTHITARHGKWLQRYQPDGCVATFIHKKLSTSGKILLLEEQDKIGLALRLNPDS
                     ALILKNIGDFFSVTTIYYKRSGLQGNEIGRYTGSSWATSPFIDRKR"
                     /name="hypothetical protein"
     gene            complement(1090..1458)
                     /locus_tag="HV193_RS27925"
                     /old_locus_tag="HV193_27945"
     CDS             complement(1090..1458)
                     /locus_tag="HV193_RS27925"
                     /old_locus_tag="HV193_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273380.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNDFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(1841..1990)
                     /locus_tag="HV193_RS27930"
                     /old_locus_tag="HV193_27950"
     CDS             complement(1841..1990)
                     /locus_tag="HV193_RS27930"
                     /old_locus_tag="HV193_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001442891.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(1984..2198)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.041"
                     /coverage="100.0"
                     /evalue="1.57e-69"
     Typing          2174..2661
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.688"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 ctacagagca cccttaccct tcacaagaat cgatggcaaa aagaatgggc gtttcgaaac
       61 gttctatcca gagagaaatc acaactttgg tagagcttgg cttactcgat aaacagccga
      121 ataaggccgg gagcaagaaa tttaaaggtc gaaacaccta tgacttaagc aagcttgttc
      181 aagtccttaa tgaaaaaaca cctgataaaa taagcagttt caaacgttat aaaaaaaatg
      241 gagaatcact agattcagag ctggagtaat cttagaaaat gccgacattc acgcaatctg
      301 gaactgggaa gtttgattat tggcttatag atggagttaa gtctttttca aaaataccgg
      361 ctaacacact gccatcaatc accgtagata tgccgatccg cttacaagtc ggaaatggtt
      421 actttggctc aacacacata accgccaggc atggtaagtg gcttcaacgc tatcaaccag
      481 atggttgtgt agcaacattc atccataaga agctcagcac cagtggaaag atcttactgt
      541 tggaagagca agataaaata ggtttagctc tcagactgaa cccagactca gctctgatac
      601 tgaaaaacat tggtgatttt ttctctgtaa caacaatcta ttacaaacga agtgggcttc
      661 agggaaatga aataggaagg tataccggtt catcgtgggc gacatctcct tttatcgata
      721 gaaagcgata accccaccaa gatttaccat aacatctctt gcgtgcaccg cctgagaggc
      781 tctcagggcg tttctgtggc ctgagccata aaaagcccca tcttctcccc gaaaacccgc
      841 cagaaacgcg ctgagagatt ttcagcccta tgcatgacta tgcaccggac taactcgcag
      901 gcgattttgg cctgaattca ggccggatga cttaaaatct gcaccaaagc caacagaaaa
      961 cggtgaacgg gtgcgcaatt tattgcgtgg ccgtgaggca ttgaccaagc cgtaaggcac
     1021 ggtaggtaat tgcaggtatc cgacaatttc gagtagagga tccactattt cactacttag
     1081 aattcaaaat cagaatcctt tcttcctgct aaggctatct ctgaatgact caaccactct
     1141 ttgagattct tcttctgctc gcatgaagtc attcccggac agaaaaaaat cattgtcagg
     1201 cccactggct tcagagaaat tcgtttctgc agcagacaca gaatccacat cagcaagaga
     1261 cggttcatct gtactttttc tcaatctgta aagagcctta tcaaacgcaa ccagtgagat
     1321 attcaggccg taacgttcat tcagcacagt cacaatctct tccctacgga taccattttt
     1381 cttccaggtt tctatatcgt tcttggaatc cctgacagct gcaatggctg ttcttggacc
     1441 agggttaaaa ttcgccatat accgcccata atgtcttaca tttgtctcat aatagtccaa
     1501 aaataagagt aaaacgcaaa aaatgtctta caattgtctt atttttgtcc tttccggttc
     1561 atgtatccat gaaccgcctg atgtggaacg ctttttatcg atctcatgcg ctgccaaaga
     1621 tagattcatt ctttaaaagt caggttacat tatcccttaa atggccatac accatacgca
     1681 accgcgattt tcatcgcaat tgcatgaatt aaaaggtaaa aaccgaattt cccgtttatt
     1741 tatgtaatac gcaacgtatt acaaccacct gtaatcacat gaaaataaag aaataagatg
     1801 taataccggc gtattacacg tacgcgggta agcgaaaaag ctataacgct tcacggccga
     1861 caggtggccc aaaatccacc ggggcgtgca ggttctcttc agtaacctgc gccattaaat
     1921 caaaaaggtc atattcgccg ggaagctgtg tatcagtcga atccagttgt tcatcatatt
     1981 cagatggcat ggggtgatat ccaatagctg agaggttgtt agagggtttc ggggcgcagc
     2041 cctgaaccag tcacgtagcg ctagcggagt gtatactggc tgaagatgtt agatcggagt
     2101 ggtaattcag agaagttatt catgttgcaa gggaaaatgc ggctataggg cgtaagtgca
     2161 aaatgcaggt aacggtgaca tgacgcctcc tcgctcactc ggtcgctacg ctcctgccgt
     2221 gaggctgcgg cgggcgttac ctgctcacga atgacaagat aaagagatgg cactcaagtc
     2281 aggaagggcc ggaaaaagga tacggcgctg ccgtttttcc ataggctccg cccccctgac
     2341 aagcatcacg aaatctgacg ctcaaatcag tggcggcgaa acccgacagg actataaaga
     2401 tcccaggcgt ttccccctgg tagctccctc gtgcgctctc ctgttcctgc ctttcggttt
     2461 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     2521 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     2581 ctgcgcctta tccggtaact atcaacttga gtccaacccg gaaagacacg acaaagcgcc
     2641 actggcagca gccataggta agaaggtgta caagaagatt actagtgcag agttcttgaa
     2701 gtctgagcca aattacggct aaactggaag gacgaatttg gtggcagcgg tcttgtacac
     2761 cctgttacca cggttcataa acttgccggt ttaatgaacc ttcgaaaaac caccttgccg
     2821 ggtggttttt tcgtttctga gactacattt ttgcttacct tcctgtcaat caagaagata
     2881 acaaacaaaa aaagctgccc ctggcagctt tttttatgag tccgagtggc gtacgtgatg
     2941 tctgtgatca gattcatcat ccctctctgt gagggagccc actcaagtgg ctgcgaaacg
     3001 cagggcgatc cactatcagc gcatgttgcc tcgccattat atcgccatga cggacaaatc
     3061 gtcttctgta agactcatag caagtatggt gacaaaacca tatcaaaaaa aggcgacaaa
     3121 atgtctatac atatgaatat acatatataa ttataaccaa gaacactcta acatcattaa
     3181 ataatgatgt tagagtgttc ttggtgtcgc tacaaattaa aaaacgaggt tcgtaatgaa
     3241 agcgaagaca gagccacttg aaaaaaagtg gggagaacga agcatgctta tgggatggac
     3301 agcgatccct acatcgctgc tttttctaca ggcggaacta tcaattagtc ctttgggttt
     3361 caatttgttg atgaacctta tttctcactg gtgggaaa
//
