LOCUS       NZ_CP056537             4772 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00433 plasmid pRHBSTW-00433_8,
            complete sequence.
ACCESSION   NZ_CP056537
VERSION     NZ_CP056537.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148610
            Assembly: GCF_013737695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00433
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.61164450012x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013737695.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 02:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,637
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,309
            CDSs (with protein)               :: 5,309
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 69 of 203
            Pseudo Genes (incomplete)         :: 143 of 203
            Pseudo Genes (internal stop)      :: 32 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056537.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00433"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00433_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4591..4772,1..136))
                     /locus_tag="HV193_RS27880"
                     /old_locus_tag="HV193_27900"
     CDS             complement(join(4591..4772,1..136))
                     /locus_tag="HV193_RS27880"
                     /old_locus_tag="HV193_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            323..565
                     /locus_tag="HV193_RS27885"
                     /old_locus_tag="HV193_27905"
     CDS             323..565
                     /locus_tag="HV193_RS27885"
                     /old_locus_tag="HV193_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(1228..1443)
                     /locus_tag="HV193_RS27890"
                     /old_locus_tag="HV193_27910"
     CDS             complement(1228..1443)
                     /locus_tag="HV193_RS27890"
                     /old_locus_tag="HV193_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1450..1941)
                     /locus_tag="HV193_RS27895"
                     /old_locus_tag="HV193_27915"
     CDS             complement(1450..1941)
                     /locus_tag="HV193_RS27895"
                     /old_locus_tag="HV193_27915"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_181469608.1"
                     /translation="MSSLLTLAKDLEQQSKARQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVSYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1976..2680)
                     /locus_tag="HV193_RS28470"
                     /old_locus_tag="HV193_27920"
                     /pseudo=""
     CDS             complement(<1976..2680)
                     /locus_tag="HV193_RS28470"
                     /old_locus_tag="HV193_27920"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2619..2942)
                     /locus_tag="HV193_RS27905"
                     /old_locus_tag="HV193_27925"
     CDS             complement(2619..2942)
                     /locus_tag="HV193_RS27905"
                     /old_locus_tag="HV193_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_029593203.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3150..3385)
                     /locus_tag="HV193_RS28475"
                     /pseudo=""
     CDS             complement(3150..3385)
                     /locus_tag="HV193_RS28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3590..>3712
                     /locus_tag="HV193_RS28480"
                     /pseudo=""
     CDS             3590..>3712
                     /locus_tag="HV193_RS28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(4032..4388)
                     /locus_tag="HV193_RS27910"
                     /old_locus_tag="HV193_27930"
     CDS             complement(4032..4388)
                     /locus_tag="HV193_RS27910"
                     /old_locus_tag="HV193_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     Typing          complement(3056..3229)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          3153..3699
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1131..2629)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2626..2942)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="2.04e-61"
ORIGIN
        1 tatccagctt gcgacctaat gaagcactaa tcgcagttgg aatctttcga ttggctttac
       61 catatttgta aaactggtat agccaatcgt ctcgaaactg aatgcggcaa tcatcatgtt
      121 catgatcttg ctgcattttt cttttacctc aatttttgcc gcaacttggc aattttttcg
      181 atcaccttct tacctccgat atagtacagc aaacatatca aatgcacaac agcacagtgc
      241 acttgtgcaa tcaatgacca tgttgtagaa tgcactcacg cacagtgcag gtgtgcaaaa
      301 taaatgataa tgtggaggtt gcatggctct tgttcgctca ggaaacaaaa gcaagatttt
      361 tgaagcagat acgaagttta actcggtttt tgacactgaa tataagccgg gagaaacatg
      421 tgaatatgaa gggatttata aatgtaccgg ttgcggtaaa gaagtaacta tcgcaggcgg
      481 gaaaacaatc ccaccacaaa accaccacca acataccccc gcgcaaggga acattcgttg
      541 gcaattgttt gtttatgctc agtgaggtat agaaaatggc agatcgtcga ccactcaacc
      601 cacacgttgt cgttcctaaa ccaggcggcg gaactcaacc tcgtgaccgt aacaatgacg
      661 gaagggtcag aggaaaacgc tcagatgcag gaaagccacg aaaataacag caagccgcca
      721 gcctggcggc ttttttatat ctgcagctga tactgattta ccataacatc cgttgcccgc
      781 accgctgaaa cggggttggc tgcgtttccg gtgccagacg cgagaaagtg gcgacagaca
      841 tgacgaaaaa acaccggaaa cgcagccgct ggatattaca cttatgcata gtcatgcagc
      901 cagatgcatg ccgcgtaaag tcacactaaa atcagcgcga acgccttaaa aaccggcaaa
      961 tgcctctccg gcaactcaca cagctccatc accaggtaaa aacagttcag cagggcttca
     1021 ataacgttga gaattttcat cacttcctcc gggataagtc actaccattc cggtagtcag
     1081 cctatttagc aggaacgcga tgagcccttc ttcgtgcggg ctgtcacctc atcttcggac
     1141 catcaaaact cttctgcttc tgtcggagcg tcagctccag ctcgttctga cgctgatact
     1201 ccagtacagc cttctcctcg atggcattca gttcccggac aagcggcgca gctgctcgct
     1261 taaactcagc acctgcgtgc tcaagtccga cacgcgctcg ttcagcatcg cgttctcccg
     1321 ttgcataagc ccacacatgc tccgccattc cgcgaaggcg ctctcccatt cgtccagcct
     1381 ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgctttt ccagctctgt
     1441 cattttccgc tactcctgtt tcaggcggat ccatgtcgtc ccgtcgtaag ggatggcttc
     1501 ggtttgcatc cccggcggca ggataagaaa cctccggccg tcgctgctct cctgatagct
     1561 cactccccac gtccgggcgg tcattttcgc cagggtatct tcctgctgac gaagatgcgt
     1621 gtaattgtcg gttatctgct gcccctgcca ccacagaatg ctcccgctcg tgccgatcag
     1681 cagcgctgac accatcagga tggtgagcca tgtccgcccg gcagtccgca gtacgctccg
     1741 ccggtttttc tccatcgcct cgctcatact ctgctcgtgg gcgaggatgg cgtcgctgat
     1801 tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc tgaatgcggc
     1861 tttcagcatc tcgccggtac tctgctgccg cgctttcgat tgctgctcta agtccttcgc
     1921 cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc cccccgtccg
     1981 ggtcggcaat gctgatactg cttttcgtgg tgcgcaccac ctcaaaacct gaccgttcca
     2041 gcgcctcagt gacatcctga cggcttttca gctccccgga cgaagcaagc gtcagcagtc
     2101 cgcgcgtgat ggcttctgcg gcctcctgct tcgttttcgg cagtccggac ggcgtgacca
     2161 gcgcccggcg gttttccggc gcgttcgggt cgtgcagccc cagtctgccg ttcacgatgg
     2221 tctgccaggc atcgatgcgc ggccggtcgg ctcggtcgta gtatggctgg agccgcctgc
     2281 cggtcagtag ctcggtattg gggatcagaa agttcagctc cagacgcccc ttgtcctcgt
     2341 gctccaccca cagaatgctg tactgatctt tatcgagtcc gggcatcaga acccgctcga
     2401 agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca aacgacagta
     2461 cgccggaagt gtatttcttc acgtaaggcg acgcgtcaat cagctcccgg acttcttccg
     2521 gcttcccctg caggacgctg gcgccttcac gctgccggtc tttacccagc agatagtcca
     2581 ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct
     2641 ttctcaagaa cggcgtgccg cagccgctca agcccggcat cgatggccat cagcgccgcc
     2701 acaatctgta ccctgtcatg gccggtgccg ccgccggcgt taacacggcg ggctatctgg
     2761 ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg ctggcgagag tgagggaagc
     2821 tttccggtgc gcgcaggctt ttcgtccagg caggtctgtc gcatccacgc cgccagctgc
     2881 ctgccgtcac agcgctcgag cagccgccgg tgctcgtcct ccgtcaccca catggtgagc
     2941 atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg gcgggaaagc
     3001 ctttcccggt cggcgcgggg ttggacaagc cgcaggcacg tcaggctttt ggcgggtttc
     3061 ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc ttactatgtt
     3121 ggcacggatg cggaaatcag tgaagtgctt catgtggaag gagaaaaaag gcggcaccgg
     3181 cgcgtaagca gaatatgcga tacaggatat attccgcttc ctcgctcact gactcgctgc
     3241 gctcggtcgt tcggctgcgg cgagcggtac cggcttactg gcggggcgga aatttcctgg
     3301 gagatgccaa gaagagactt aacagggaag cgataaggcc gcggcggagc cgtttttcca
     3361 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     3421 cccgacagga cttaaagata ccaggcgttt ccccctggtg gctccctcgt gcgctctcct
     3481 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc
     3541 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     3601 ccccgttcag tccgaccgct gcgcctgttc cggtaactat cgacttgagt ccaacccgga
     3661 aagacacgac aaaacgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctggaaggac
     3781 agtatttggt atctgcgctc tgctgaagcc agttaccagg ttaagcagtt ccccaactga
     3841 cttaaccttc gaaaaaccgc ctccccaggc ggttttttcg tttacagagc aagagattac
     3901 gcgtacagag cacggatctc aagaagatcg taagaagaaa gttaacatga gcgtttttat
     3961 ccctcaaaaa gaaggcttct ataactagaa gccttctttt tgagggataa cttatatttt
     4021 tttaaaaaaa atcatgcaat agcatgtttc ttctcagaac tatcaacaat gtgtttcctt
     4081 tctatgattt catgagcggg aatcactctg tctatttctt cttgcaatcg agggctacta
     4141 gtaacctccc ataaatcaac agactgctgt atatttagcc agaactttgc agtggtatcg
     4201 aaggctttag ccaacctaaa agccatatcg gtagacagct ttctattatt gttaactaaa
     4261 gcactggcag agtttctatg cacattcaac atttcagcca agtcattgat ttttaaacca
     4321 cttggttcga gatattcata aagaataata tcccctacag ttgatggttt tctagttgct
     4381 tgggacatgc tttactccta aattttttac ttctctctgt tagatgagga agataagtat
     4441 ttcgtctatt accagctgta gcgatgagta attatcttaa atatagtcac gtcatttttt
     4501 ttattaatca ttatttgtaa tgacatttta atgtaggtga gggaacgcct cacctacttg
     4561 ttttataaga taacagccac gacctgttat ctatcggtaa tcatgtggat cgagatacaa
     4621 atcctcgact ccgttttcac cccacttaaa tatcaagcgg tacttctcat tcacccgtat
     4681 ggatgaatac cctgtcaatg gagggttgag attctcaaat cggtttcccg ggggagaccg
     4741 caaatctcca actttatcag cagcattcag ca
//
