LOCUS       NZ_CP056536             5586 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00433 plasmid pRHBSTW-00433_7,
            complete sequence.
ACCESSION   NZ_CP056536
VERSION     NZ_CP056536.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148610
            Assembly: GCF_013737695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00433
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.61164450012x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013737695.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 02:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,637
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,309
            CDSs (with protein)               :: 5,309
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 69 of 203
            Pseudo Genes (incomplete)         :: 143 of 203
            Pseudo Genes (internal stop)      :: 32 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056536.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00433"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00433_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(606..1211)
                     /locus_tag="HV193_RS27860"
                     /old_locus_tag="HV193_27880"
     CDS             complement(606..1211)
                     /locus_tag="HV193_RS27860"
                     /old_locus_tag="HV193_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            1306..4203
                     /locus_tag="HV193_RS27865"
                     /old_locus_tag="HV193_27885"
     CDS             1306..4203
                     /locus_tag="HV193_RS27865"
                     /old_locus_tag="HV193_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4287..4589
                     /locus_tag="HV193_RS27870"
                     /old_locus_tag="HV193_27890"
     CDS             4287..4589
                     /locus_tag="HV193_RS27870"
                     /old_locus_tag="HV193_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(4849..5085)
                     /locus_tag="HV193_RS27875"
                     /old_locus_tag="HV193_27895"
     CDS             complement(4849..5085)
                     /locus_tag="HV193_RS27875"
                     /old_locus_tag="HV193_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(5251..5424)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          19..514
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.0"
                     /coverage="99.0"
                     /evalue="6.33e-132"
     Typing          5397..5582
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.513"
                     /coverage="94.0"
                     /evalue="4.15e-69"
ORIGIN
        1 atctgacgct caaatcagtg gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc
       61 cccctggtgg ctccctcgtg ctcccccgca ttcctgcctt tcggtttacc ggtgtcattc
      121 cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgc gaaggcagtt
      181 cgctcctgga tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc
      241 ggtaactatc atcttgagtc caacccggaa agacacgact tatcgccact ggcagcagcc
      301 actggtaact gatttagagg agttagtctt gaagttatgc gcctgttaag gctaaactga
      361 aagaacagat ttggttactg cgctcctcgt aagccagtta ccacggttca aagagttgct
      421 gctctgagaa ccttcgaaaa accgccttgc agggcggttt tttcgtttac agagcaagag
      481 attacgacgc cacagaaagg atctcaagaa gatccttcac cgtaatggaa atctatccaa
      541 tccgagcaca taggatacac atcgttataa aaggggtcgg ttccggctga gggcgaaatg
      601 acaccctaag cgttagctct gtgtcgttgc acgatgtcag cgacggtatt cttgctgata
      661 ccgagctcgc gtgcgatcca gcgatagctg cgtccctcgg ccctcatcgc aaccacctta
      721 ggcaaaagtc ggtctgattt tggtcgcact ccggcctgac gaccaagcct cttaccacgt
      781 gccttcgcaa cagcaaggcc tgacttgacc cgctcgctga tgagatcccg ctcaaactcc
      841 gcaatgccgg aaagaaacgt cgccagcatt cgtccatacg gcgacgaaag atcgaacgcc
      901 attccattca tggctatcac ggaaaccttc cagttctcca gttcacgtag cgtattgagc
      961 agatcgagcg tcgagcgccc ccaccgggaa agctcagtga ccaggattgc atcaatttgt
     1021 ctggactggg caagcgccag gactttcttt cgctcggccc ggtcgagttt agttcctgaa
     1081 cctgtttcct taaatattcc caccacgtcg tagccggcac ggccggcgaa ggctcgcaga
     1141 tcaaattcct ggcgttcaca agactgatcc gctgttgaaa cccggcagta aatggcggca
     1201 cgatgtccca attgaaccct cctggatttt tgtatcggaa cgccctgatt tatatgggct
     1261 ggctgttgtc caaaacagac tatacttcaa aagggacgaa tttgtatgtc acgacgccat
     1321 attttcaccg aacggcagcg agcagcgctg ttcgatctgc ccacggacga actgtcgcta
     1381 ctgaagttct acacgctggg cgatgatgac ctggaaaaca ttaggcagcg ccgcagaccg
     1441 gaaaacagga ttggctttgc cctgcaactt tgtgccttac gatatccggg ccgtgcactg
     1501 gctcctggtg agatgatccc gcgtgaagtc ctttccttcg tcggtgctca gcttggagtt
     1561 ccggctgatg cgcttctcac ttatgccaca cggcgccaaa cccgtcagca gcacatggac
     1621 acgctgcgcg aaatttacgg ctacaagacc ttcacgggcc gtggtgcccg tgatctgcgg
     1681 gagtggactt tcggccaggc cgaagatgcc agatcaaacg aggatcttgc tcatcgtttt
     1741 attgtgcggt gtcgggaaac ttccaccatt ctgcccgcag tatcgacaat cgagcgcttg
     1801 tgcgcggatg ctctggtcgc cgctgagcgg cggattgaaa cgcggattgc ggaaaattta
     1861 acagcggatg ttcgcgatca cctggacaaa cttctgagtg aaatgctcgc cggcaatatc
     1921 agtcgtttca tctggcttcg caacttcgag gttggtaaca actcggctgc tgctaaccgt
     1981 ttgctcgaca ggctcgaatt tctgcgtacc ctgaatatca atcatagtgc tttggccagc
     2041 atacctgccc atcgcattgc ccggctgcgt cggcagggtg aacgctactt caccgacggt
     2101 ttgcgtgaca tcacttcgga ccgccgctgg gcgatccttg ccgtctgtgt tgtggagtgg
     2161 gaagcggcga ttgctgatgc catagtcgaa acccatgaca ggatcgtagg aaaaacctgg
     2221 cgggaagcga agcgccagca tgacgaaaca atttccggct ctaaagccac actcacggat
     2281 acgatccgta ccttcaccgc gctgggagct tcgttgcttg aggcccgcag tgacggaacc
     2341 ccgctggaga tggctgtcgc cagttcggtt gcatgggacc ggctcgctca actggtagcg
     2401 acagggactc aactcagcaa cacgctagcc gatgagcctc ttgcatatgt cgggcaggga
     2461 taccatcgct ttcgtcgtta tgcgccccgc atgttgcgct gtctgaagct cgaagccgcg
     2521 ccggtcgccg gaccattggt agcagcagct ttgtcgatcg gagagatgaa aggtgttgca
     2581 tcgccagaaa ggcgtttcct gcggcccagc tccaaatgga accgtcattt acgagctcag
     2641 gaaaaaggag atacccgtct ttgggaagtg gcggtactct ttcacctccg ggatgctttt
     2701 cgttccggag atgtctggct cgctcattcg cgccgctatg gtgacctcaa gcaggtactg
     2761 gtgccgatga tcgcggcgca ggaaaatgca aaactggccg tgccttccaa cccacaggat
     2821 tggctggcag acagaaaggc gcgactcacg atcgctctta agcggctggc ccgggctgcc
     2881 cgtaacggca ctattccgca cggtagcata gaagatggaa cgttgcggat cgacaggttg
     2941 acagcagacg tgccggatgg tgccgaggca ctcatactgg atctgtatcg ccgaatgccg
     3001 tccgttcgga ttaccgacat gctgcttgaa gttgatgcag cccttggttt cacagatgcg
     3061 tttacccatc tgagaaccgg ggctccatgt cgcgaccgga tcggtctgct caacgtcctg
     3121 ctcgctgaag ggctcaatct gggcctgcgt aagatggcgg aagctacaaa cacgcatgat
     3181 tactggcagc tctcacgcct tgcccgctgg catgttgaaa gcgaagccat gaaccaggca
     3241 ttggcaattg tggtggccgc gcagggtaaa ctgccgatgt cacgcgtctg ggggatgggc
     3301 acgtcagcat cgagcgatgg tcagtttttc ccgacagcgc ggcatggcga agccatgaac
     3361 atggtcaatg ccaaatatgg ttctgttccc ggcctcaaag cgtatactca cgtaagcgac
     3421 cagttcgcgc cattcgcttg tcagtcgatc ccggcgaccg tgagcgaggc accgtatatt
     3481 ctcgatggac tactgatgaa cgaggtcggt cgccatgttc gcgaacagta tgccgataca
     3541 gcaggattca ccgaccattt gttcggagcc agtagcctgc tcggctacaa tctcgttctg
     3601 cgaatcaggg atctgccatc gaagcggttg tacgtattta atcccgatac gacccccagg
     3661 gagttacgca agttggtagg tggaaaagcc cgggaggatc ttatcgttgc gaactggcct
     3721 gatattttcc gttgtgccgc gacgatgacc gctggcaaaa tcaggcccag ccaactcctg
     3781 cgcaagctcg cttcttaccc acgacaaaac aaccttgcag ttgcgcttcg tgaagttggt
     3841 cgtattgaac ggaccctttt cattattgag tggatcctgg atacggacat gcagcggcgt
     3901 gctcagatcg gtcttaacaa gggagaggcc caccatgcgc tcaaaaatgc gctccgtatc
     3961 gggaggcagg gggaaattcg cgatcgcacg acagaggggc agcactaccg aatcgctggg
     4021 ctcaatttat tgactgcggt gatcatttac tggaataccg tccatcttgg tcatgccgtc
     4081 acggagcggc ggaacgaagg gttggatgtt ccccctgaat ttcttcccca catatcccca
     4141 ttgggctggg cgcacattct actgactggc gaatatcttt ggcccaagga accgaaagct
     4201 tagggtgtca tttcgccctc agccggaacc gacccctaaa acatcggata ttatgatccc
     4261 aattaaaata agctcgagga taaaaaatga gttcaatggt ttactgccga ggatgtggca
     4321 aggaaataca cgaaacagca aagtcatgcc cgcactgcgg agccacaaac gcctcttcag
     4381 gttcaggtga aaaaagtaga atcgcagccg cgctattagc attcttcctt ggtggatttg
     4441 gcgcacataa attttactta ggaaaaatag gacaggggtt cctgtatcta attttttgct
     4501 ggacattcat acccgcgatc atcgcgttta ttgagtttat tatttatcta tgcgattccg
     4561 atgaaaaatt cgcacgaaaa tatggctaac tcatagcaaa aaaaagcccc ctaagggctt
     4621 ttttttgtac ctgacggtca gaccgattaa acataacatc ccttgcccgc acccccgcag
     4681 gcgccctcag cgcaatcctg ctgcatcagg gccgtttcca gcacctttcc tcaccagtat
     4741 cccggaaaaa cgcccctgat gatttctgcg cgactgcatg gctatgcacg caggtgaatg
     4801 gcaggtgatt tcgcagcgtc agcaccacta aaccgccaaa agccaaaatc agcggcgcgg
     4861 cgcgcgcgga cgtgaccagg caaaatcgga ctgctgccgc tcatcctcac gtaatgcacg
     4921 ctggcggtcg gcgtaacgct gcaactcaat ttgcgcacgc gctctgttca ggacgatgtg
     4981 ccggttaggc tgatttagct ttttacctga cgctctttca gctgcggcaa cagcatcatc
     5041 aagaaatttt ttctctgcag gcgtcaagtt tgtcagtctg gccataacgt tctccagtca
     5101 gaataaatca gcacagtctg ccagacacag tagaaacgat gaagcgccag cgacggtgaa
     5161 aggcagtcag aaacggtgaa cgggagcgga ttttaatccg gtgccgtgag gcgttggaca
     5221 agccgcaggc gcgtcattag cttttagcgg gtttcggggc gcagccctga accagtcacg
     5281 tagcgctagc ggagtgtata ctggctgact atgttggcac ggatgtggaa atcagtgaag
     5341 tgcttcatgt agcaggagaa aaaaggcggc accagcgcgt cagcaggata tgtagtacag
     5401 gatatattcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5461 ggtgccggct tacaggcggg gcggaaattt cctgggagat accaggaagg agcttaacag
     5521 ggaagagata aggccgcggc gaagccgttt ttccataggc tccgcccccc tgacaaacat
     5581 catgaa
//
