LOCUS       NZ_CP056534             6995 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00433 plasmid pRHBSTW-00433_5,
            complete sequence.
ACCESSION   NZ_CP056534
VERSION     NZ_CP056534.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148610
            Assembly: GCF_013737695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00433
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.61164450012x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013737695.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 02:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,637
            CDSs (total)                      :: 5,511
            Genes (coding)                    :: 5,309
            CDSs (with protein)               :: 5,309
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 69 of 203
            Pseudo Genes (incomplete)         :: 143 of 203
            Pseudo Genes (internal stop)      :: 32 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00433"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00433_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            285..476
                     /locus_tag="HV193_RS27790"
                     /old_locus_tag="HV193_27810"
     CDS             285..476
                     /locus_tag="HV193_RS27790"
                     /old_locus_tag="HV193_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            476..877
                     /locus_tag="HV193_RS27795"
                     /old_locus_tag="HV193_27815"
     CDS             476..877
                     /locus_tag="HV193_RS27795"
                     /old_locus_tag="HV193_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714819.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADHSEQVISHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1669..2274)
                     /locus_tag="HV193_RS27800"
                     /old_locus_tag="HV193_27820"
     CDS             complement(1669..2274)
                     /locus_tag="HV193_RS27800"
                     /old_locus_tag="HV193_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            2369..5266
                     /locus_tag="HV193_RS27805"
                     /old_locus_tag="HV193_27825"
     CDS             2369..5266
                     /locus_tag="HV193_RS27805"
                     /old_locus_tag="HV193_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(5282..>5542)
                     /locus_tag="HV193_RS27810"
                     /old_locus_tag="HV193_27830"
                     /pseudo=""
     CDS             complement(5282..>5542)
                     /locus_tag="HV193_RS27810"
                     /old_locus_tag="HV193_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <5541..6024
                     /locus_tag="HV193_RS27815"
                     /old_locus_tag="HV193_27835"
                     /pseudo=""
     CDS             <5541..6024
                     /locus_tag="HV193_RS27815"
                     /old_locus_tag="HV193_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            6026..6283
                     /locus_tag="HV193_RS27820"
                     /old_locus_tag="HV193_27840"
     CDS             6026..6283
                     /locus_tag="HV193_RS27820"
                     /old_locus_tag="HV193_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            6391..6546
                     /locus_tag="HV193_RS27825"
                     /old_locus_tag="HV193_27845"
     CDS             6391..6546
                     /locus_tag="HV193_RS27825"
                     /old_locus_tag="HV193_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(6751..6864)
                     /locus_tag="HV193_RS28465"
     CDS             complement(6751..6864)
                     /locus_tag="HV193_RS28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049026061.1"
                     /translation="MHEPPVQSDCCALSGNCHLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(2..164)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="99.0"
                     /evalue="4.19e-53"
     Typing          complement(6550..6995)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.473"
                     /coverage="99.0"
                     /evalue="1.8099999999999999e-112"
ORIGIN
        1 tcagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
       61 cgtcaaattc tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ccgctgggcg
      121 gcctcttctt ttccgcctcc cttgatcgct cggttttctc gagcttttat aagaacggtc
      181 ttgccgctcg ccgcagccga acgaccggag cgtagcgact gagtgagcga ggaagcggaa
      241 aagagattgt tcttacactg agcactgacg ctctgaggct tcttatgacg aaacaacaga
      301 aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg ctggagaagc
      361 ttaacgagct tgatctcgat gtagaggccg acctttgtga gaaactccac gatgatgcgg
      421 agcatctttt ccgtacactg tcctcgagac tggatgcatt acaggatggt aactgatggc
      481 cagagtgtct attagtgagg ctgcccgact gacgggtaag agtagaacaa cactgcacag
      541 gttaattaaa gcaggtgacc tgtccacctg tagcggtgaa cgcaatgcaa aaatggtaga
      601 tacttcagag cttttacgtg tctttggtcc ctttgaacaa ccgaagggtg aacaccattc
      661 tggacaggtc tctgaacagc gtgatacagc ctctgctgac catagtgaac aggtgattag
      721 tcatctgaaa caggagattg aacacctgaa taccttactt ttgtccaagg attcgcacat
      781 tgacagcctg aaacaggcca tgctccttct tgaaaatcac caggcaaaat taactgaatc
      841 cccggcgcca tggtggaaat tctggaaaaa atcctgaccc cgtcatgctt ccgttttcct
      901 ctccggccat cccggggagt tcctgaacct tcccaaaaat cgccagatct ctgaacgctc
      961 ctcacgacta aaaataccca cacactacgc aaaattaaaa ttttgcagat tccttattta
     1021 aaacaattgg ttaatgaaaa attaatgtgc agtgatgtgt tacaaagtgc agatccaagt
     1081 cgtggacgac tctatggcga atcgtttacg aattgcccac gattcatatg tgaatcacgc
     1141 tttattatca tgttgttatg atgaatcgac tcttgattca attcatcaat ttgggcgttt
     1201 tttgttcatg gtgactcttg attcaattca cgattcgaat cgccggtgaa tcgtgtggtg
     1261 agtcgtccgg cgacttgcca taacgtgatt gccggccgtc gacgaacccg attcctgaac
     1321 gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcctc acggccacgc aataaattgc
     1381 gtccccgttc accgttttcg gatcagaaga acgccggaaa caggacttac cctggctgaa
     1441 tggcgtgaat ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt aaaaacagag
     1501 cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca
     1561 gaaacgtcct gaggccgttt tagcggtgcg tacaattaag ggattatggt aaatggtgac
     1621 ttggttaatc ataaaggggt cggttccggc tgagggcgaa atgacaccct aagcgttagc
     1681 tctgtgtcgt tgcacgatgt cagcgacggt attcttgctg ataccgagct cgcgtgcgat
     1741 ccagcgatag ctgcgtccct cggccctcat cgcaaccacc ttaggcaaaa gtcggtctga
     1801 ttttggtcgc actccggcct gacgaccaag cctcttacca cgtgccttcg caacagcaag
     1861 gcctgacttg acccgctcgc tgatgagatc ccgctcaaac tccgcaatgc cggaaagaaa
     1921 cgtcgccagc attcgtccat acggcgacga aagatcgaac gccattccat tcatggctat
     1981 cacggaaacc ttccagttct ccagttcacg tagcgtattg agcagatcga gcgtcgagcg
     2041 cccccaccgg gaaagctcag tgaccaggat tgcatcaatt tgtctggact gggcaagcgc
     2101 caggactttc tttcgctcgg cccggtcgag tttagttcct gaacctgttt ccttaaatat
     2161 tcccaccacg tcgtagccgg cacggccggc gaaggctcgc agatcaaatt cctggcgttc
     2221 acaagactga tccgctgttg aaacccggca gtaaatggcg gcacgatgtc ccaattgaac
     2281 cctcctggat ttttgtatcg gaacgccctg atttatatgg gctggctgtt gtccaaaaca
     2341 gactatactt caaaagggac gaatttgtat gtcacgacgc catattttca ccgaacggca
     2401 gcgagcagcg ctgttcgatc tgcccacgga cgaactgtcg ctactgaagt tctacacgct
     2461 gggcgatgat gacctggaaa acattaggca gcgccgcaga ccggaaaaca ggattggctt
     2521 tgccctgcaa ctttgtgcct tacgatatcc gggccgtgca ctggctcctg gtgagatgat
     2581 cccgcgtgaa gtcctttcct tcgtcggtgc tcagcttgga gttccggctg atgcgcttct
     2641 cacttatgcc acacggcgcc aaacccgtca gcagcacatg gacacgctgc gcgaaattta
     2701 cggctacaag accttcacgg gccgtggtgc ccgtgatctg cgggagtgga ctttcggcca
     2761 ggccgaagat gccagatcaa acgaggatct tgctcatcgt tttattgtgc ggtgtcggga
     2821 aacttccacc attctgcccg cagtatcgac aatcgagcgc ttgtgcgcgg atgctctggt
     2881 cgccgctgag cggcggattg aaacgcggat tgcggaaaat ttaacagcgg atgttcgcga
     2941 tcacctggac aaacttctga gtgaaatgct cgccggcaat atcagtcgtt tcatctggct
     3001 tcgcaacttc gaggttggta acaactcggc tgctgctaac cgtttgctcg acaggctcga
     3061 atttctgcgt accctgaata tcaatcatag tgctttggcc agcatacctg cccatcgcat
     3121 tgcccggctg cgtcggcagg gtgaacgcta cttcaccgac ggtttgcgtg acatcacttc
     3181 ggaccgccgc tgggcgatcc ttgccgtctg tgttgtggag tgggaagcgg cgattgctga
     3241 tgccatagtc gaaacccatg acaggatcgt aggaaaaacc tggcgggaag cgaagcgcca
     3301 gcatgacgaa acaatttccg gctctaaagc cacactcacg gatacgatcc gtaccttcac
     3361 cgcgctggga gcttcgttgc ttgaggcccg cagtgacgga accccgctgg agatggctgt
     3421 cgccagttcg gttgcatggg accggctcgc tcaactggta gcgacaggga ctcaactcag
     3481 caacacgcta gccgatgagc ctcttgcata tgtcgggcag ggataccatc gctttcgtcg
     3541 ttatgcgccc cgcatgttgc gctgtctgaa gctcgaagcc gcgccggtcg ccggaccatt
     3601 ggtagcagca gctttgtcga tcggagagat gaaaggtgtt gcatcgccag aaaggcgttt
     3661 cctgcggccc agctccaaat ggaaccgtca tttacgagct caggaaaaag gagatacccg
     3721 tctttgggaa gtggcggtac tctttcacct ccgggatgct tttcgttccg gagatgtctg
     3781 gctcgctcat tcgcgccgct atggtgacct caagcaggta ctggtgccga tgatcgcggc
     3841 gcaggaaaat gcaaaactgg ccgtgccttc caacccacag gattggctgg cagacagaaa
     3901 ggcgcgactc acgatcgctc ttaagcggct ggcccgggct gcccgtaacg gcactattcc
     3961 gcacggtagc atagaagatg gaacgttgcg gatcgacagg ttgacagcag acgtgccgga
     4021 tggtgccgag gcactcatac tggatctgta tcgccgaatg ccgtccgttc ggattaccga
     4081 catgctgctt gaagttgatg cagcccttgg tttcacagat gcgtttaccc atctgagaac
     4141 cggggctcca tgtcgcgacc ggatcggtct gctcaacgtc ctgctcgctg aagggctcaa
     4201 tctgggcctg cgtaagatgg cggaagctac aaacacgcat gattactggc agctctcacg
     4261 ccttgcccgc tggcatgttg aaagcgaagc catgaaccag gcattggcaa ttgtggtggc
     4321 cgcgcagggt aaactgccga tgtcacgcgt ctgggggatg ggcacgtcag catcgagcga
     4381 tggtcagttt ttcccgacag cgcggcatgg cgaagccatg aacatggtca atgccaaata
     4441 tggttctgtt cccggcctca aagcgtatac tcacgtaagc gaccagttcg cgccattcgc
     4501 ttgtcagtcg atcccggcga ccgtgagcga ggcaccgtat attctcgatg gactactgat
     4561 gaacgaggtc ggtcgccatg ttcgcgaaca gtatgccgat acagcaggat tcaccgacca
     4621 tttgttcgga gccagtagcc tgctcggcta caatctcgtt ctgcgaatca gggatctgcc
     4681 atcgaagcgg ttgtacgtat ttaatcccga tacgaccccc agggagttac gcaagttggt
     4741 aggtggaaaa gcccgggagg atcttatcgt tgcgaactgg cctgatattt tccgttgtgc
     4801 cgcgacgatg accgctggca aaatcaggcc cagccaactc ctgcgcaagc tcgcttctta
     4861 cccacgacaa aacaaccttg cagttgcgct tcgtgaagtt ggtcgtattg aacggaccct
     4921 tttcattatt gagtggatcc tggatacgga catgcagcgg cgtgctcaga tcggtcttaa
     4981 caagggagag gcccaccatg cgctcaaaaa tgcgctccgt atcgggaggc agggggaaat
     5041 tcgcgatcgc acgacagagg ggcagcacta ccgaatcgct gggctcaatt tattgactgc
     5101 ggtgatcatt tactggaata ccgtccatct tggtcatgcc gtcacggagc ggcggaacga
     5161 agggttggat gttccccctg aatttcttcc ccacatatcc ccattgggct gggcgcacat
     5221 tctactgact ggcgaatatc tttggcccaa ggaaccgaaa gcttagggtg tcatttcgcc
     5281 ctcagccgga accgacccca taaacatcct gggggatggc atacaccagt tggatatccg
     5341 gtgcatcgtt gagccatcga cctgattttt tcattaagta gccaccaatt tcttcggtag
     5401 tcagccgggc atgaaacaat gcttcatcat tgaaccagca gtcagggtga tagatgagat
     5461 ccagtgggtt tgccttgaaa ttaatctcca gtaactggag tgcatagctg tggtcagatg
     5521 cactaccttc tgctttgcgt atggcggatg acctcgattt tgacgatatt attctggtgt
     5581 ttcctccgga atccggccta aaaccgctgt atgtgatgta ccgtagctct cgtaacatgc
     5641 ccggtacagt gagcggcaaa gggcagaacg ttggcaataa ctggatgggg gggggccagt
     5701 accggggatg gtgctcctgt tccttcccag attgcggata aattacgcgg gaagactttc
     5761 ggtagttttg attctttccg tcgggcgttc tggaaagcgg ttgctgacga ttctgcactg
     5821 agcaagcaat ttagtgaagc tgatattaat cagatgaaag ctggaagggc acctactgcg
     5881 gactttttag aatcagtagg gaaacgtgtt aaaattgaat tacaccacga aaaagaaata
     5941 tctcaaggag gggctgttat ggatgtcgat aacattaagg cgctaacacc taaaaatcat
     6001 attgagacac ataaaggaaa ataaaatggc tggtaataaa atttcagatt ttacggaaga
     6061 agaatttact gcttttgtta gtaaaataat cgctgctgat tttccaactg acaaagagaa
     6121 cgatgatgct atttattcgt tcgcccaact aacagagcat cctgctggtt ggaacctcat
     6181 cttcaagcct aaggtaggcg aagattcttc ggcgaaagga attgttaaaa ccgtaaagga
     6241 atggcgagct gctaacggta agccaggttt taaaaaagcg tgattgaatt tgcgggttca
     6301 agttatgagc ccgctcattt gtaactctgg atttgaagaa taattactag atatccattt
     6361 gcctagaata ataaaaataa aaggtgatat atgaaaaaat tacttttcct aggggttgtt
     6421 accgtttctg tggttctggc agcatgtcag gtcaataatg ttcgagacac cggaggtggg
     6481 gctgtttcac cttcatcgac ggtgaccgga gtcacgcttg atagcggtac gaacggcaat
     6541 ccgtaaagga tcttcttgag atcccatttg gatcgtcgta atctcttgct ctgtaaacga
     6601 aaaaaccgcc ttgcagggcg gttttttcga aggttcgagg agttggcgct ctttgaaccg
     6661 aggtaactgg cttagaggag cgcagtaacc aaattcgttc tttcagttta gccttaactg
     6721 gcacataact tcaagactaa ctcctctaaa tcagttacca gtggctgctg ccagtggcgc
     6781 ttttgcatgc ctttccgggt tggactcaag atgacagtta ccggataagg cgcagcagtc
     6841 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     6901 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     6961 gaatggcagg aacaggagag cgcacgaggg agcca
//
