LOCUS       NZ_CP056514             2694 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00446 plasmid pRHBSTW-00446_7, complete
            sequence.
ACCESSION   NZ_CP056514
VERSION     NZ_CP056514.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148616
            Assembly: GCF_013737455.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00446
  ORGANISM  Citrobacter sp. RHBSTW-00446
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00446
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.9836083515939x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 15:00:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,699
            CDSs (total)                      :: 5,580
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 39 of 185
            Pseudo Genes (incomplete)         :: 142 of 185
            Pseudo Genes (internal stop)      :: 27 of 185
            Pseudo Genes (multiple problems)  :: 20 of 185
            Pseudo Genes (short protein)      :: 1 of 185
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056514.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Citrobacter sp. RHBSTW-00446"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00446"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742648"
                     /plasmid="pRHBSTW-00446_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            37..642
                     /locus_tag="HV199_RS28295"
                     /old_locus_tag="HV199_28305"
     CDS             37..642
                     /locus_tag="HV199_RS28295"
                     /old_locus_tag="HV199_28305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            1163..1489
                     /locus_tag="HV199_RS28300"
                     /old_locus_tag="HV199_28310"
     CDS             1163..1489
                     /locus_tag="HV199_RS28300"
                     /old_locus_tag="HV199_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_228288709.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1512..2528)
                     /gene="mobV"
                     /locus_tag="HV199_RS28305"
                     /old_locus_tag="HV199_28315"
     CDS             complement(1512..2528)
                     /gene="mobV"
                     /locus_tag="HV199_RS28305"
                     /old_locus_tag="HV199_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_167593153.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     Typing          complement(1516..2528)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attgattagt acattaactc tggtgaggaa agtaagatgt cattcataaa atcgctaaac
       61 aaaaaaaatc aaacagacca aatagaagtg acgattaacg ttaggttaac ccaagaagag
      121 gaccatttac tgacattagc aacccagcaa tctggttata aaaggaacga gctattctac
      181 ctcctagcaa aggaatacat ctttgatgat gaaagcttaa aggaaatggc tgaggagcct
      241 ggcgctgagt tttcagaagg cttataccaa aattgttatg tggttaacac taacagagtt
      301 catcatgaag aagatgataa gttcatgatt tcagagggcg tagcggcaac atttgaagat
      361 ggttataagg aaaaaatgga gcaggttaaa aaaggagatt tagtctttct ttatcgctcc
      421 ggcgaaggaa ttgttgcaca tggtatcgcc agtggtgatg ttttaaagaa agatcataat
      481 ggcgtgccag acaagaccta ctaccagaag ctatcaggct ttgttaggta cgaaaaacca
      541 ctaaccgcgt ctgagatcca gagaatactg gggagaaacg ttgtgtttat gcacacgcta
      601 tttagcatta gagatggcta taaactgctt aaaaaagatt aaacccagtc tacaggtggt
      661 tggcatcact ttgtgacacc aaccacactt ccggaagtgt tttttacagt gcatcgtcaa
      721 gggtaaatga actcgctacg ctcgcccttg acgaaataaa gctccgaata tcgttttaaa
      781 aaatcaagaa ccgctcgccg cagactcatg accgaacgta gtgagcgaat gagcgaggaa
      841 gcggaatttc cacgtcactt acataggaac cctaacactc agaacaggca taaaagcacc
      901 tgattttgcc gatgtgtgat tgtggagagt aataagatac agaaggggat ggctgggaag
      961 ccgcgccaca agcggaaagt ttttgacaat agttatctct agagataact tagttatctt
     1021 gagagataac tattttttcc tgaatggaga tttttagatg cggagaatca cgcaggtaga
     1081 ccaaactaca ggggaggaat taggtggttt tgtagcagtg attcgtccta aacaaaagtc
     1141 atcattccag aggcacttta ctatgaacca agccgcgcta attaccattg ccaatgagct
     1201 aaaccatgac cagatgcgtg tgctgatggc cttgctcgca gaactggatt acgagaacta
     1261 catccaagtt gcgcagatgg atattgcgga agcgctgact atgcaaaaaa caaatgtcag
     1321 tcgtgcggta aaaaacctga ttgatttcgg gatcatactg gaaggtccta agataggacg
     1381 gagcaaaact tatcgcttga atccccagtt cggttggaaa ggtactgtca gcaaccacaa
     1441 aaaagcacta aaaaacggcc tcagcgttat acagggcggt agaagctgat ctctggaatg
     1501 tcgcatcaaa actaacgact cataccaccc atgccacgcg atcgcgtctg tcgctgtttt
     1561 tcacgttcaa tacgctgctg tttttccttt tcctggtttt gctgcttaaa ccgctgagcc
     1621 atcatcagga tttcggtcat ctgctctctg gaaaggccct taaaaggctc tgtaacgctc
     1681 tgtaggcgtt tctgctgcga gtccatagtc ttctgtagct cgcgcactct ttttgcattc
     1741 tgcgcgctct gagaggccat ggcgatggtt cctgcaaggc catcgttcac tgcgtgactt
     1801 agacgtttgg ctatggctgc ctgatcttcc acgtctttgg tgaaaattcc tttccgcaat
     1861 acgcgcggct ccagcgcatc tggcgagatt gatacctggc tacgggtgcc acggtttatc
     1921 gattggtagt agtgctgaac tgtctgatgc gtggcacggc ttccctcgat gccgcgctca
     1981 agacccaatt tttttactga ttcagcgtag gtgctctgat cgtcacgcat ctttgaccgc
     2041 ccgccaataa actctttcgc ggacagccgt ccatcctgcg tcaatggcac cacgaacgct
     2101 gacaaatgcg gggtggcctc gtcccggtga actaccgcag ccacaacacg atccttcccg
     2161 tatttcttct ccagccactg ctcagaatgg gcgaaaaact ccgcctgctg ccgcggtgtc
     2221 gcttctttcc accactcagg gcttgccgtc ataacgtact caacagccag taccgcatct
     2281 ttacggcgtt tttccggcaa cagctcccgc agctttccca tcgcctcatc tgccgactga
     2341 gacacagagc agtagttttc tggtgtccgt tcagcgtcag cattcggcgt ctcacgctcc
     2401 ctgtagcagt gctgcaaagc gctggccaca ctgcccattc ccgtcagctt tttgcagcgc
     2461 attatcgcgt aattcgccat gcttaccccc tcgttctggc gtacatccag ccagtcgggg
     2521 gatcgcattg cgatgcctcc ggcgggccac tatcaggatg atagtgtact cactagactt
     2581 tgctacgcaa aatcgttcgt taaattcaac ttaataaaaa atatatgaca taaagtcatt
     2641 gcttaaatca aagatgtgat caatgcttct gtttcaatgt agtgattcac tatc
//
