LOCUS       NZ_CP056513             2746 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00446 plasmid pRHBSTW-00446_6, complete
            sequence.
ACCESSION   NZ_CP056513
VERSION     NZ_CP056513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148616
            Assembly: GCF_013737455.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00446
  ORGANISM  Citrobacter sp. RHBSTW-00446
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00446
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.9836083515939x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 15:00:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,699
            CDSs (total)                      :: 5,580
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 39 of 185
            Pseudo Genes (incomplete)         :: 142 of 185
            Pseudo Genes (internal stop)      :: 27 of 185
            Pseudo Genes (multiple problems)  :: 20 of 185
            Pseudo Genes (short protein)      :: 1 of 185
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056513.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Citrobacter sp. RHBSTW-00446"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00446"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742648"
                     /plasmid="pRHBSTW-00446_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(124..489)
                     /locus_tag="HV199_RS28275"
                     /old_locus_tag="HV199_28285"
     CDS             complement(124..489)
                     /locus_tag="HV199_RS28275"
                     /old_locus_tag="HV199_28285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181627509.1"
                     /translation="MSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSLLGQ
                     GFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKELNP
                     DFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            827..1015
                     /locus_tag="HV199_RS28280"
                     /old_locus_tag="HV199_28290"
     CDS             827..1015
                     /locus_tag="HV199_RS28280"
                     /old_locus_tag="HV199_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1063..1290)
                     /locus_tag="HV199_RS28285"
                     /old_locus_tag="HV199_28295"
     CDS             complement(1063..1290)
                     /locus_tag="HV199_RS28285"
                     /old_locus_tag="HV199_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228710239.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLENVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            <2580..2681
                     /locus_tag="HV199_RS28290"
                     /old_locus_tag="HV199_28300"
                     /pseudo=""
     CDS             <2580..2681
                     /locus_tag="HV199_RS28290"
                     /old_locus_tag="HV199_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          1764..2259
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.97"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 aatgctatca ataatgcttt gcctaaagca ggaataaaaa agccacctac acggtggctt
       61 tttttctgtc cgggaactag ctgaattgaa cctaaaatcc cggccacgaa ctggtagaca
      121 cctttatccc tgaagatgtt tgaagagaaa acggcctatt tcataggtcg taaagtcagg
      181 gtttagctct ttatcatcca tccctacagc gctccctgcc tcgatcaata ccttcttcat
      241 gttgctaact ttaagcccgt ctttggtaac caagatcttt ccttttggaa gcttatgaat
      301 cacgtaatcc ttgaaggaat gtgtgtcctt atctccacca ggcaaagaaa agccctgacc
      361 taataacgat tttaatgaat tattctcttc aattaagttc aggacaacat catcaaccgt
      421 catgccaaag tcattcacgt actcttttag gctctcaata gttgatttct ttagtcttac
      481 gtcagacata ccattcatat tattattacc ccaccgctct aatttgaaga aataatacaa
      541 caaaatacaa attatacaaa taaatattgt tctgaataca acaagcggtc tgtaggtgcg
      601 atgataacta tgccgcaccg ccagattgaa cataacatcc gttgcgtgca ccgcctgaga
      661 ggctctcagg gcgtttctac gacctcacct gcaaaagaac acatcttacc ccctgaaaac
      721 cgctcagaaa cgcgctgatg atttctgcgt acctgcatgg ctatgcactc aagtgaatga
      781 agtaagattt cagtctgcga tcaccataaa accgggaaaa agccgtatga caaaacagga
      841 atcagccgca ctgaacatgg caaaatttat ccgggcgcag tcgctgctgc tgctcgaaaa
      901 actcgacgtg ctcgatcttg atgacgaggc agccgactgt gagcgaatgc acgaacaggc
      961 agaagcgctt tatcagaagc tgagcgcacg cttcggtata caggacggcg aataaagaga
     1021 cgaaaagaac gctcacttag acgaaaagcg tatccagggc aactatgtat atccaccgtg
     1081 tacatagtca tgcctgacgt tttcgagcag gtcgagcatg taggaacgac gagcttgatt
     1141 ggtatcggac acaatatggc gatacgcctc aaacataaca gcacccgcat caccgcccga
     1201 acggcgaagc atagcggaaa tcatcctcac aaagagcaga cgttcatcgg ggcaaaggtt
     1261 cataagttct cctggtgaaa aaccaaacat gaaagcaccc atctattaac gtcagctgtg
     1321 aacgcttcat tagaagaaaa taggtaaacc cgggaaaaag atccaaacgt taacatcaca
     1381 ggtaaacaga aacgtcgggt cgatcgggaa attctttccc ggacggcgcg gggttgggca
     1441 agccgcaggc gcgtcagtgc ttttagcggg tgtcggggcg cagccctgaa ccagtcacgt
     1501 agcgctagcg gagtgtatac tggctaaact tcagagatgc caaggaaggg acaggatatg
     1561 ttttgtagca ccgccaccac ggtggcgata ctccaaaacg cctggtcagg gcacagcccc
     1621 gacaccccag agccgactgt aggtcactgg acgaacgcgg cggaatgatg tcggatgacg
     1681 tcattttgcg gcgtttgccc tatcctgcat cgtattaaaa aatgcagcta tggcgcgtac
     1741 ggtgccaaag ataagcccgg agatattccg cttcctcgct cactgactcg ctccgctcgg
     1801 tcgttcggct gcggcgagcg gtaccggctt actggcgggg cggaaatttc ctggcatctt
     1861 ccaggaagaa gcttaacagg gaagcaagaa ggccacggag aagccgtttt tccataggct
     1921 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     1981 aggacttaaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct
     2041 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca ttccacgcct
     2101 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     2161 tcagtccgac cgctgcgcct tgtccggtaa ctatcgtctt gagtccaacc cggaaagaca
     2221 cgacttaacg ccattggcag cagccattgg taagaggatt cacggagcag ggtagtagag
     2281 tgcttctaca gagttcttga agtggcggcc tgactgcggc tacactggaa ggacagtttt
     2341 ggtatctgcg ctccgtgaaa gccagttacc ttcggaagaa agagttggta gctcttgatt
     2401 ccggcaaacg aaccaccgtt ggtagcggtg gtttttttgt ttacaggaca tgagattacg
     2461 acgagagtaa aaggatcaca agaagatccg gctgtttacc agtgatttgg cttcagtatt
     2521 ggtttccgcc tagcggggcc ttatacgtaa aaattccaca cctgacttat cacacagggg
     2581 agctccgcaa gtaccagaga gcactgaata gcaggccacg gaaatgtctt ggattccggc
     2641 aaccttcagt ggtctttgct gagctaagac aggcggctta gcgttgcact tcgaacttga
     2701 atccgcccag aagcattcag cagaagtcgc taacggtttg cttgca
//
