LOCUS       NZ_CP056491             3994 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella sp. RHBSTW-00464 plasmid pRHBSTW-00464_9, complete
            sequence.
ACCESSION   NZ_CP056491
VERSION     NZ_CP056491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148621
            Assembly: GCF_013736875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. RHBSTW-00464
  ORGANISM  Klebsiella sp. RHBSTW-00464
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00464
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.7478063554263x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,650
            CDSs (total)                      :: 6,530
            Genes (coding)                    :: 6,329
            CDSs (with protein)               :: 6,329
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 64 of 201
            Pseudo Genes (incomplete)         :: 152 of 201
            Pseudo Genes (internal stop)      :: 38 of 201
            Pseudo Genes (multiple problems)  :: 46 of 201
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Klebsiella sp. RHBSTW-00464"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00464"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742649"
                     /plasmid="pRHBSTW-00464_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3799..3994,1..659))
                     /locus_tag="HV204_RS33090"
                     /old_locus_tag="HV204_33085"
     CDS             complement(join(3799..3994,1..659))
                     /locus_tag="HV204_RS33090"
                     /old_locus_tag="HV204_33085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            complement(1196..2389)
                     /gene="mobQ"
                     /locus_tag="HV204_RS33095"
                     /old_locus_tag="HV204_33090"
     CDS             complement(1196..2389)
                     /gene="mobQ"
                     /locus_tag="HV204_RS33095"
                     /old_locus_tag="HV204_33090"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            2632..3021
                     /locus_tag="HV204_RS33100"
                     /old_locus_tag="HV204_33095"
     CDS             2632..3021
                     /locus_tag="HV204_RS33100"
                     /old_locus_tag="HV204_33095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121527125.1"
                     /translation="MNKLITEAEGAKIAGVFKYILEASESILIDHGHVITGQIIKNKKF
                     FADNGHKDFEKLEYYLGSAISSLVDNKVIEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNIRGMQKAFNIFGGDK"
                     /name="hypothetical protein"
     gene            3018..3302
                     /locus_tag="HV204_RS33105"
                     /old_locus_tag="HV204_33100"
     CDS             3018..3302
                     /locus_tag="HV204_RS33105"
                     /old_locus_tag="HV204_33100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_079843899.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(3539..3802)
                     /locus_tag="HV204_RS33110"
                     /old_locus_tag="HV204_33105"
     CDS             complement(3539..3802)
                     /locus_tag="HV204_RS33110"
                     /old_locus_tag="HV204_33105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     Typing          complement(1380..2389)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tttaagccct gtgcctttaa tagtagttcg ttttcgaatt tctcgaaagt ggatattttt
       61 ttgaaattta aaaccgttct gtaagcatat tgccgtagat cttcaaacaa attaacattg
      121 cgacctaaac cataagcctc gctttttttc ttttgtattt gaggcttttc taagtccaca
      181 aaatcagcta aataatccag gtcataaagg tcagcacctg accagaacgg cgaccagtga
      241 tcattgagtg ggttttttgt aatcaatcca gcaaacccca gatctgcttt tagttttttc
      301 gccatagcca tttcaacggc tgcggcatac ttcaacggct ttaaccttgc ttgttgtgtc
      361 ttacaaaccc cggctttcag catatagccg tagtgacaac gacctgtttt tggtgttttt
      421 gtaataaagt gaggtatggg taagcccacc tgctcaaatg tatagaatga atttttctta
      481 tctagatcaa agagtaagca agtttgaatg gctggctgat ttgcctgtaa atacagcatt
      541 tcaagggctt ttgccttgtt ccttatgctt aatcccatat cgaggttatt tgtacaataa
      601 ggcttgttag gcatttgatt tttaaaaaaa tcgatgttat gttcttcttg tattatcatt
      661 ttttcagtcc tttttatctg gccctactct ctgttttctg tcgccaaaac aaaatcgcag
      721 agggtggggt ttttattttt atagattttt caatcctgta ccagttctaa ccgatctcaa
      781 aaatttgtca agtgtgcgtc taaccatatc agataacagc ccaacgaagt tggggcagtt
      841 ctttacatac ggcagcaaag cattactatc agatctatgg tgcgaagcat taccgaagat
      901 agaaaaacaa taaaaaaaga aaaaagagta ttgttcgaag aaaaattggc gattcgattt
      961 tttttggtga atatatgaaa aaaaagaaag tgcagaaaaa acggaaaaga aataagcata
     1021 gtaaattttt taaaatgctt atgaagtcag ttaatgaagc tgtggcaata cataaagggg
     1081 aaatagaacc ggcgaggatc acaactctaa aaatgtagta tcttatagta tagtattaaa
     1141 taatattaag ttgtattaag tgatactact atataggaat atataccact atatactaca
     1201 ttccaaattt cttcttcctc ttaggttgct gatcttcgaa ttccggcatt ggttccggct
     1261 caggctcttc aatttttggt tgtggttggt tcataggaga cattccagag cctgaaccca
     1321 tttttccgcc tccattattt ccaccggttc cggttcctgt gcctgtttta ggttttgttt
     1381 ctaactctgt ctttaatata tgttcgccaa attcatttat tacataacta ctaacgaaat
     1441 tttgaggatc ttctttttct tcttttttat ttttatttac attaggtttt gatttaaata
     1501 tatgctgggt ctttttgtat aatgccttta aattatcaat gattgacctt gcaagaggct
     1561 ttttctctat tttcatagtt tcctctggct gaggtggttt ggtttgctgt aagtatgttg
     1621 cattagctat ttttaataat aatgcttttt ctttttgctc catagctctt aatttttgac
     1681 cttttcgcca ttggtgcctg tgtatcctgg tcattggcgt tctgtccagt tcaatagttt
     1741 ttgaaatcca cttggctttc tctttcggag ttttagcaaa aacagccttt ctttctgcct
     1801 cttctttttg gtcttttaat gaacgggagt caatacgaga tttatgacca tattttttta
     1861 atcggtcatt tgctattatt tcccagttaa ctcgccaggc ttcaatgttt tctctattat
     1921 tccattctct ttgtttgttc ccaaatccat ttgggctaac agttcttaaa gttagcatta
     1981 catgagcatg agggttatga ctatcaagat catgaaatgc tatgtctgca atcattccat
     2041 aatccacgaa attttcttgg cagtaatcga ggacgatctg cttcttctgg ccgtcgtcca
     2101 gctccttggg gatggctatg tccagataac ggcataattc ggcatccttt cgcttctcgc
     2161 cagcctctac ggcattccag agggtgcctg agtccttcac cagttcggca ggtgtgccgg
     2221 caggggagag gatagcagag ccgaaaaatc cctctttttt tgaataatcc caggttgccc
     2281 cggttcgatt atcttttgtc ttttctcggt tttgatatgc agataaataa accgaagatt
     2341 taccttctga tcttcgtaat tttttaaagc tgatatgaaa tgtcgccata gtctacccct
     2401 tacttttttt gtcttttgaa ttttggtttt tagccccgca gggcccatac gtcgcttgcg
     2461 acgtgtaagt gggcattact aatattattt tttcggcttc acctcaaaat aataagtaat
     2521 gaaccccagg cttcgcccaa tattaaatat tatttatttt attctaaaat atatttgact
     2581 aaagtaaata atggaggtaa taaataacaa aataaataat taggaggttt tatgaataaa
     2641 cttataacag aggcagaagg tgcaaaaatc gcaggtgttt ttaaatacat attagaggca
     2701 tctgaaagta tattaataga tcacgggcat gttatcacgg ggcaaataat aaaaaacaaa
     2761 aaattctttg ctgataacgg gcataaagat tttgaaaaat tggagtatta tcttggttca
     2821 gcaattagtt ctcttgtcga taataaagta attgaaggta ttaaagatat taacacggat
     2881 gagaaccttg tttttaaaaa aataaatgaa ttgagtgata aggaaaaatt aattttagct
     2941 gagtgtatta ttaaacattt tgaatattca aatattagag gaatgcaaaa agcttttaat
     3001 atttttgggg gtgataaatg agtaaatcta ctgcacagca gatcgcagat ttgcaaaata
     3061 aactacaagc attaaggaca aaaaaagcaa gacaggaccg aagggaagaa acgagacaaa
     3121 aaataatcct gggtgcagaa gtggctaagg ttttaggtaa aaaaccagag gaaattaaca
     3181 aggctttaat tcttgggttg ttaagtaaaa taaatgaact tgacgaagaa gaaaaacaag
     3241 aattcacacg attaggatta aatattttag aagagtggaa gaatgaaaat cgtaaaccgt
     3301 gaacaataaa aaaccagccg atcttggctg gtttttcatt gtgaagggtt gccagccgta
     3361 ggctggaggt tttaaggttg ccagagggcc tctggtggct gggtgggttt gttgatttgt
     3421 tggtaccaga gtgtccctca cctatccaga cacaagcccg gatacgggga aaggggaaaa
     3481 aatcccccca ggggtggagg gtttggttag gttaggggga aaaactgctt agaagtcgtt
     3541 acaggctgtt ttgaggcata tctaaggcaa cataacggcg atacagggca acggctccgt
     3601 gatacgaaca gcccattttg tcggctactt gttgccattt cagcccctgg cttcgtagtt
     3661 cgtaagcctg acgacgacga tccattgctg ttttttcgta atcgtcacga gattgagaaa
     3721 aatattttct tacagttctt tcactacgac ctgtcatttc cattatttct tttgctttta
     3781 tttttttacc attaatgatc ataaaacccc cttgctcatt aatatgtcta acatttcatt
     3841 tttaacattg gtttttcgag gttttttggc tctgtttgat tggatttgtg aaaatgtatg
     3901 gctatcaaaa tttttccaag tccaacgggc tacacttcta actgttgctt ttatctcttt
     3961 gtattgtaac gggttttgtg gattgcaaaa agtg
//
