LOCUS       NZ_CP056490             4423 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella sp. RHBSTW-00464 plasmid pRHBSTW-00464_8, complete
            sequence.
ACCESSION   NZ_CP056490
VERSION     NZ_CP056490.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148621
            Assembly: GCF_013736875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. RHBSTW-00464
  ORGANISM  Klebsiella sp. RHBSTW-00464
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00464
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.7478063554263x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,650
            CDSs (total)                      :: 6,530
            Genes (coding)                    :: 6,329
            CDSs (with protein)               :: 6,329
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 64 of 201
            Pseudo Genes (incomplete)         :: 152 of 201
            Pseudo Genes (internal stop)      :: 38 of 201
            Pseudo Genes (multiple problems)  :: 46 of 201
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056490.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Klebsiella sp. RHBSTW-00464"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00464"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742649"
                     /plasmid="pRHBSTW-00464_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1..894
                     /locus_tag="HV204_RS33060"
                     /old_locus_tag="HV204_33055"
     CDS             1..894
                     /locus_tag="HV204_RS33060"
                     /old_locus_tag="HV204_33055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080816845.1"
                     /translation="MHNAALGLFNDRLPHKPYFSDDLHFGVRIAGKERALLAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETSIRTAPDGRMK
                     PLKYAAAVENALRKKLDADTGYSGLLCKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NNKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNLQFSSPLD
                     DNEVIGIAKSIAKWTRANFSEGTFSDYVFRTHSSEIQSLRGRKSKGGGRPSLNEVWVDL
                     GVSRRTYFRMKSKLKG"
                     /name="replication initiation protein"
     gene            complement(924..1706)
                     /locus_tag="HV204_RS33065"
                     /old_locus_tag="HV204_33060"
     CDS             complement(924..1706)
                     /locus_tag="HV204_RS33065"
                     /old_locus_tag="HV204_33060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080901937.1"
                     /translation="MENHYEYLKLDMSASIDEIRDAIELNKNNDEARIRKIKSILLNER
                     FKSEYDEKLISYLINNGDSSRKADEKKQSLYIEQIKNLDNGELHNSYIYAAFFLLVLNF
                     SLSLVVSTQVDYVISFIVNILTIFVLYKDWKILEKNNLAVFSKWWVLISPVYLFKRSNA
                     KKESKKLFSLWMAIIIIFVASSLIFAGGKSTVESAACGVVSDIYRTQFHQPGLTCKSVT
                     ITESEGKNHYGIAEISDGSVKDITIKELSDGQIYVTVE"
                     /name="hypothetical protein"
     gene            2054..2377
                     /locus_tag="HV204_RS33070"
                     /old_locus_tag="HV204_33065"
     CDS             2054..2377
                     /locus_tag="HV204_RS33070"
                     /old_locus_tag="HV204_33065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2586..>3020
                     /locus_tag="HV204_RS33495"
                     /pseudo=""
     CDS             2586..>3020
                     /locus_tag="HV204_RS33495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3055..3546
                     /locus_tag="HV204_RS33080"
                     /old_locus_tag="HV204_33075"
     CDS             3055..3546
                     /locus_tag="HV204_RS33080"
                     /old_locus_tag="HV204_33075"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_044715458.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3553..3768
                     /locus_tag="HV204_RS33085"
                     /old_locus_tag="HV204_33080"
     CDS             3553..3768
                     /locus_tag="HV204_RS33085"
                     /old_locus_tag="HV204_33080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2368..3860
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcataacg ccgcactcgg actttttaac gaccgactcc cgcacaagcc gtacttctcg
       61 gacgatctgc acttcggtgt gcgtatcgca gggaaagaac gcgcactctt ggcaaagtac
      121 atccagttta atcagccgca tgccatgttc tggctgggct tcgacgtcga tcgcgccggt
      181 gcggccatcg actggagtga ccggaacgcg cccgcgccca cgctgaccat caccaacccg
      241 gaaaacgggc acgcgcacct gctatacgca ctggaaacct cgatacgtac tgcgccggac
      301 ggcagaatga aacctctgaa gtatgcagct gccgtggaaa acgcgctgcg taaaaaactg
      361 gatgctgata cagggtattc tggcctgctc tgcaagaatc cgaaccacgg tcactggaaa
      421 attgccgttt ggcagccaga actctacacg ctggactggc ttgctgattt cctcgatctg
      481 aacgcggcaa acaacaaaga gatcgttgcc gactatggct taggccgcaa ctgcactctg
      541 ttcgataaaa cacggaagtg ggcttatcgc gctatccgtc agggctggcc tgaatatgac
      601 cagtggctgc aagcttgcta tgaacgtgcc agtgcttaca acctgcaatt ttcatcgcct
      661 ctggatgata atgaggtgat tggcatagct aaaagtattg cgaaatggac tcgtgctaat
      721 tttagtgaag gtactttcag tgattatgtt tttcgtacgc attcatctga aatacaaagc
      781 ctccgaggga ggaaaagcaa aggtgggggg aggccgtcat taaatgaagt gtgggttgat
      841 ttgggtgtta gcagaagaac atatttcaga atgaagtcaa aactaaaagg ttagtggctg
      901 ggatcaacca gccactaact ttgttattca acagtgacgt atatttgccc atcactaagt
      961 tctttgatag tgatatcttt tacagatccg tcagatatct cagctatgcc atagtgattt
     1021 ttcccttcac tttctgtgat cgtcacagat ttgcaagtta gtcctggttg atggaactgt
     1081 gttctgtata tgtcgcttac gactccacag gctgcacttt ccactgtaga cttaccgcca
     1141 gcaaagatta agctactagc aacaaaaata atgattatcg ccatccacaa agagaagagt
     1201 tttttacttt ctttctttgc attggatctt ttaaataaat aaacaggtga tattaatacc
     1261 caccacttag aaaatacagc aaggttgttt ttttctaata ttttccagtc tttataaagc
     1321 acaaaaatgg tcaatatgtt cactatgaaa ctaattacat agtctacttg tgtgctaacc
     1381 actaatgata aagagaagtt taaaaccaac aggaaaaacg cggcgtatat gtatgagtta
     1441 tgcaactccc cattatctaa gttttttatc tgttcaatgt ataaactttg ttttttttca
     1501 tctgcttttc ttgatgagtc accattattt atcaagtatg atattaattt ctcatcatat
     1561 tcacttttga atcgctcatt aagtaagatt gattttatct tccttattct agcttcatca
     1621 ttatttttgt ttagctcaat agcatctctg atttcatcga ttgaagcaga catgtcaagt
     1681 ttcaggtatt cgtaatgatt ttccatgtaa ttccttgcag taagtacctt ctaaaaggtt
     1741 gattatacgc attaagaata gtcttaaaaa gggttctcgg cagttggcac taaacaacct
     1801 atatcagata acagccctct tttgtcagat ggctgttttt tgcagcaatt cctactcagt
     1861 catgggagcg cagcgtcacc attaagccag tatacactcc gctagcgcta cgtgactggt
     1921 tcagggctgc gccccgaaac ccgccaaaag cctgacgcgc ctgcggcttg tccaaccccg
     1981 cgccgaccgg gaaaggcttt cccgcccgtc ccggcgttat caggaggccg gtttgtcaga
     2041 gaaacggagc aagatgctca ccatgtgggt gacggaggac gagcaccggc ggctgctcga
     2101 gcgctgtgac ggcaggcagc tggcggcgtg gatgcggcag atctgcctgg acgaaaagcc
     2161 ggcacgttcc ggaaagctcc cctcactctc gccggcgctg ctgcgccagc ttgccggcat
     2221 gggcaataac ctgaaccaga tagcccgccg tgttaacgcc ggcggcggca ccggccatga
     2281 cagggtacag attgtggcgg cgctgatggc catcgatgcc ggccttgagc ggctgcggca
     2341 cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccgcgc ggccgcggcg
     2401 gtggcgccgg gccggtggac tatctgctgg gtaaagaccg gcagcgtgaa ggcgccaccg
     2461 tcctgcaggg gaagccggaa gaagtccggg agctgattga cgcgtcgcct tacgttaaga
     2521 aatacacctc cggcgttctg tcgtttgcgg aggccgatct gcctcccggc cagcgggaaa
     2581 aactgatggc cagcttcgag cgggttctga tgcccggact cgataaagat cagtacagca
     2641 ttctgtgggt ggagcacgcg gacaaggggc gtctggagct gaactttctg atccccaata
     2701 ccgagctgct gaccggcagg cggctccagc cttactacga ccgcgccgac cggccgcgca
     2761 tcgatgcctg gcagactatc gtgaacggca gactggggct gcacgacccg aacgcgccgg
     2821 aaaaccgccg ggcgctggtc acgccgtccg ggctgccgaa aacgaagcag gaggccgcag
     2881 aagccattac ccggggactg ctgacccttg cttcgtccgg ggagctgaaa agccgtcagg
     2941 atgtcactga cactcttgaa ggtgcaggtt ttgaggtggt gcgcaccacg aaaagcagta
     3001 tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc atctatgagc
     3061 agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagca gcagagtacc
     3121 ggcgagatgc tgaaagccgc attcagcgag cacgagcggt ctgtcagagc ggaactgagc
     3181 gcaagcgcga ggagaatcag cgacgccatc ctcgcccacg agcagagtat gagcgaggcg
     3241 atggagaaaa accggcggag cgtactgcgg actgccgggc ggacatggct caccatcctg
     3301 atggtgtcag cgctgctgat cggcacgagc gggagcattc tgtggtggca ggggcagcag
     3361 ataaccgaca attacacgca tcttcgtcag caggaagata ccctggcgaa aatgaccgcc
     3421 cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct tatcctgccg
     3481 ccggggatgc aaaccgaagc catcccctac gacgggacga catggatccg cctgaaacag
     3541 gagtagcgga aaatgacaga gctggaaaag cagttgctga gcgcattaga gcagctacag
     3601 caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg gcggagcatg
     3661 tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga cttgagcacg
     3721 caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat gccaccgagt
     3781 tccggcagag ggagaatgag tatcagaaaa cactggagct ggagcaccag ccgcagaaaa
     3841 cctaccgtgg tccaacgctg ggctgacggc ccgtgacaaa ccccaagaag aagggctcat
     3901 aaatttcccg ctaaatacgg tcgtgatgga aacatcacag cgtatacaac ggagggatta
     3961 tgagactgtt taacgtgatt gagacggtgc tgaactgcct gtacatggcg ctggagatct
     4021 gggactggct ggagaagcac tttcctgttt ttaaggcgtt cgcgctgatt ttagtgtgac
     4081 tttacgcggc atgcatctgg ctgcatgact atgcaccagt gtaatattca gcggctgcgt
     4141 ttctggtgtt ttttcgttat gtctgtcgcc actttctcgc gtctggcacc ggaaacgcag
     4201 ccaaccccgt ttcagcggtg cgggcaacgg atgttatggt aaatcgtggt gagatcgaca
     4261 gaagcctgcg gacggtgtaa cgttaacccc agaaaaaaca aagccccgga aatcatgtca
     4321 tatcttggcg gatcgcatga ttaccagggc taggtcgaaa ccttgaaagg atattagcac
     4381 atgaaccgtg ccagaaaaca gcccgccata agcgtagggc gca
//
