LOCUS       NZ_CP056489             4678 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella sp. RHBSTW-00464 plasmid pRHBSTW-00464_7, complete
            sequence.
ACCESSION   NZ_CP056489
VERSION     NZ_CP056489.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148621
            Assembly: GCF_013736875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. RHBSTW-00464
  ORGANISM  Klebsiella sp. RHBSTW-00464
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00464
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.7478063554263x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,650
            CDSs (total)                      :: 6,530
            Genes (coding)                    :: 6,329
            CDSs (with protein)               :: 6,329
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 64 of 201
            Pseudo Genes (incomplete)         :: 152 of 201
            Pseudo Genes (internal stop)      :: 38 of 201
            Pseudo Genes (multiple problems)  :: 46 of 201
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056489.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Klebsiella sp. RHBSTW-00464"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00464"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742649"
                     /plasmid="pRHBSTW-00464_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4319..4678,1..132))
                     /locus_tag="HV204_RS33030"
                     /old_locus_tag="HV204_33025"
     CDS             complement(join(4319..4678,1..132))
                     /locus_tag="HV204_RS33030"
                     /old_locus_tag="HV204_33025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_048293775.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQML
                     DNYMSIREQKDALEKLNARTWGVTYQESSDGRRFLSMPKGTEPQIIPYEGTNWVLLKQG
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(810..1133)
                     /locus_tag="HV204_RS33040"
                     /old_locus_tag="HV204_33035"
     CDS             complement(810..1133)
                     /locus_tag="HV204_RS33040"
                     /old_locus_tag="HV204_33035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_177897834.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2194..3750)
                     /locus_tag="HV204_RS33045"
                     /old_locus_tag="HV204_33040"
     CDS             complement(2194..3750)
                     /locus_tag="HV204_RS33045"
                     /old_locus_tag="HV204_33040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_048293778.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYALKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHSLFSDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KEINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(3824..4090)
                     /locus_tag="HV204_RS33050"
                     /old_locus_tag="HV204_33045"
     CDS             complement(3824..4090)
                     /locus_tag="HV204_RS33050"
                     /old_locus_tag="HV204_33045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048293777.1"
                     /translation="MKHTGSRSGQRSIIKRWSWNDRSGSGRMTVRRCNKPDKTGLSAGI
                     CAQSLIHSPGRMAMHSGEIFQAQAARFAGEFTAGFSGLTPHSR"
                     /name="hypothetical protein"
     gene            complement(4097..4312)
                     /locus_tag="HV204_RS33055"
                     /old_locus_tag="HV204_33050"
     CDS             complement(4097..4312)
                     /locus_tag="HV204_RS33055"
                     /old_locus_tag="HV204_33050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_048293776.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLSWLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1249..1421)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          1346..1898
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(3..820)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.802"
                     /coverage="99.0"
                     /evalue="1.27e-178"
ORIGIN
        1 gctttcgttc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat
       61 ctcgccggta gtctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa
      121 aagactgttc atagatggct cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa
      181 tgctgatgct gcttttcgtg gtgcgcacca cctcgaaacc tgcgttttcc agcgcctctg
      241 taacgtcctg ccgcgttttc agctccccgg atgaggcaag ggcaagcaga ccgcgcgtaa
      301 tcgcctgggc tgcctcctgc ttcgcttccg gcagcgcgga aggcgtgacc agcgcccgcc
      361 ggttttccgg cgcgttcggg tcgtgcagcc ccagcctgcc gttcactatg gtctgccagg
      421 catcaatgcg cggacggtcg gcgcggtcgt aatacggctg gagacgtctg ccggtcagca
      481 gctccgtgtt cgggatcagg aagttcagct ccagacgccc tttgtcctgg tgctcaaccc
      541 acagcacgct gtactggtct ttatcgagtc cgggcatcag aacccgctca aagctcgcca
      601 tcagcttttc gcgctgcccg ggcggcaaat cctgctcggc aaaggacagg acgccggatg
      661 tgtacttttt ggcgtaaggc gaggcgtcga taagctcacg gacttcctcc ggcttcccct
      721 gcagaacgct ggcgccgtcg cgctgccggt cttttcccag cagataatcc accggaccgg
      781 cgccaccgcc gcgccctctg ggatgaaatt taacgatcat catcagcccc cttttccagc
      841 acggcgtgcc gcagccgctc gagtccggca tcaatggcca tcagtgcggc gacaacctgc
      901 acgcgatcgt gcccggtacc gccaccggca ttaacctgcc gggcaatctg gttgaggttg
      961 ttacccatgc cggcgagctg acgaagcagc gccggcgaga tcgaaggaag tttgccggca
     1021 cgagcgggct tctcgtccag gcacgtctgg cgcatccagg ctgcgagctg tttaccgtcg
     1081 cagcgctcca gcagtcgacg gtgctcatcc tcggtaaccc acatcgtgag cattttgctg
     1141 cgtttgtctg ccaatccggc ctcctgataa cgccgggacg gacgggaaat cttttcccgg
     1201 tcggcacggt gttggacaag ccgcaggcgc gtcagtggct tttagcgggt ttcggggcgc
     1261 agccctgaac cagtcacgta gcgctagcgg agtgtatact ggcttactat gttggcacgg
     1321 atgcgagtgt cagtgaagtg cttcatgtgg caggagaaaa atgcagcagc ggagcgtcag
     1381 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct gcgctcggtc
     1441 gttcggctgc ggcgagcggt accggcttac aggcggggcg gaaatttcct gggagatgcc
     1501 aggaagaagc ttaacaggga agcgataagg ccacggcgaa gccgtttttc cataggctcc
     1561 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg
     1681 cctttcggtt taccgatgtc aatccgctgt tatggccgcg tttatctcat tccacgcctg
     1741 acactcggtt ccgggaaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     1801 cagcccgact accacgttgt cccggtaact atcatcttga gtccaacccg gaacagacac
     1861 gaaaaatcgc cagtggcggt agccattagt aagaggattc acggagcgga gtagtagagt
     1921 gttctacaga gttcttgaag tggtggcctg actgcggcta cactggaagg acagttttgg
     1981 tacctgcgct ccgtgaaagc cagttacctt cggaagaaag agttggtagc tcttgattcc
     2041 ggcaaacgaa ccaccgttgg tagcggtggt ttttttgttt gcagggcatg agattacgac
     2101 gacatcgaag ggataacaag aggatccgct tcttgaaccc ccctccccac aaaaaaaacg
     2161 gcacactagc gcgtaaagcg tcatcaaaaa aactcaaata gcgctatata caatcacttt
     2221 attacgcccc tctctcttag cttgataaag agcatcgtca gcatttttca gcaaaaaatc
     2281 gatcccacca agaggatcat cttgtgttgc cacaccagca gatacggtga cacgcaagtt
     2341 attagggaaa acagtacgtt caataacact cctaatacgc tccgcaacac actccgctgt
     2401 taccacagat gtattcggca gaaaaacaac aaactcttct ccaccaaacc gacaaataat
     2461 atcttcacta cggcaacaag acttaataat ctgagccaaa gaaacaagca cctcatctcc
     2521 gaccccatgc ccgaaactgt catttatctc tttaaaaaaa tcaacatcaa tagcaataac
     2581 tgaagctcct gccccatact tgttattctg tattttagtt gaaaacccca atctattgtt
     2641 cacgccggta aggggatctt taagcgccac ctcatttaaa gaatcaaccc tttccatcat
     2701 taccttaaca tgcgtaaaaa gtgccttttt aagtcgatca gcttcggcat accaagcatt
     2761 gatagcttta atataaacta gcgaagctcc tatatcttta gcattggtag atatagcaag
     2821 cttttctaaa ggaagtgaaa tactggaggc aatgaaatat gaaataagtg aaaacacaat
     2881 aattattata attagcagaa caagtacact tttagcataa gcaataagaa tagtagtcac
     2941 actgtcagca cgactataaa ccaacacatt ccaagcagta tttttcatac tagcataacc
     3001 aagaagatat ctatgccccg aagaaaaaaa tgccccctca cctctatcag acgcagttaa
     3061 ctttgatttc aaatcatcag acataaccat aggccgatca acagcacttt tatcgttatt
     3121 aaagatcaca ttcccatcat cactaacaat gctaatttca gtgttgttct tgaaaaaatg
     3181 cctgctcaaa acatcgctaa ataaactatg tttttttaga tatatagacc caccgacata
     3241 ccctatgtaa ttgccaccct tatcataaat aggatgtgac aacaaaataa tcaaattccc
     3301 agtaactgat ttgaacggtg aagatatgaa tggtttcttt tcctcaatcg ccaatttgct
     3361 ggcacttgaa ttcaagcggg taccaactaa atcaagagat tctggcgaag tcgccagaac
     3421 aacagcatca ttactaacaa caaaaactga attaaacata ccagactgca atcttaacct
     3481 atccgcctca tctttaagtg agtcaatatc attagttgat tttatttttt ttgctccata
     3541 tgctaattca ccttgagcca tttcaatata accactcatt acatctgaca ttttcaaagc
     3601 gtacgatgaa ttagcataaa gcaaatcttt ttctatattt tcgcgctgaa aaaccataat
     3661 aacggcaatc agaaaaatag aagttaacaa aatcccacca acggacaagg ttatgactat
     3721 cggacgaagg tgaggctttt taattctcat aaaaatacac tccatataat aaaaaatgcc
     3781 gcatttacca taacatccct tgcccgcacc cccgcagggc ccttcagcgc gaatgcggcg
     3841 tcagcccgct aaaaccggca gtaaactccc cggcaaaccg cgcagcctgt gcctgaaaaa
     3901 tttcacccga atgcatagcc atgcggccgg gtgaatggat caggctttgg gcgcagatgc
     3961 ccgccgaaag ccccgtttta tccggtttgt tacagcgacg gaccgtcata cgtccgctgc
     4021 cgctgcgatc gttccagctc cagcgcttta tgatgctgcg ctgcccgctc ctgctgccgg
     4081 tgtgcttcaa tcgcgttcag cctttcgaca accagcttag ttgctcgctc aaactctgca
     4141 cctgcctgct caagcccgtg acgcgctcgc tcagcgccgc gttctcccgt tgcataagac
     4201 cagacatcgt ccgccattcc gcgaaggcgt tctcccactc gtccagcctt ttcgagtagt
     4261 cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctctgtc atacacagtc
     4321 acccctgttt cagcagaacc cagttcgtcc cctcgtaggg aattatttgc ggttccgttc
     4381 ctttcggcat gctcaggaac cggcggccgt cgctggattc ctgataagtc acgccccacg
     4441 ttctcgcgtt cagcttctcc agagcgtcct tctgctcccg gatgctcatg taattgtcga
     4501 gcatctgctg cccctgccac cacagaatgc ccgcgctcga cgctatcagc agcgcggaca
     4561 ccaggacgat ggtcagccac gtctggctga ccatgcgcac catgcctttc gtgcgctggc
     4621 tcatggctga ggacagcttc cggtcgtggt cgaggatggc ggcgctgatt ctcttctc
//
