LOCUS       NZ_CP056488             6785 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella sp. RHBSTW-00464 plasmid pRHBSTW-00464_6, complete
            sequence.
ACCESSION   NZ_CP056488
VERSION     NZ_CP056488.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148621
            Assembly: GCF_013736875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. RHBSTW-00464
  ORGANISM  Klebsiella sp. RHBSTW-00464
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00464
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.7478063554263x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:23:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,650
            CDSs (total)                      :: 6,530
            Genes (coding)                    :: 6,329
            CDSs (with protein)               :: 6,329
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 64 of 201
            Pseudo Genes (incomplete)         :: 152 of 201
            Pseudo Genes (internal stop)      :: 38 of 201
            Pseudo Genes (multiple problems)  :: 46 of 201
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056488.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Klebsiella sp. RHBSTW-00464"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00464"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742649"
                     /plasmid="pRHBSTW-00464_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6001..6785,1..196)
                     /locus_tag="HV204_RS32985"
                     /old_locus_tag="HV204_32980"
     CDS             join(6001..6785,1..196)
                     /locus_tag="HV204_RS32985"
                     /old_locus_tag="HV204_32980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019450.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            178..420
                     /locus_tag="HV204_RS33660"
     CDS             178..420
                     /locus_tag="HV204_RS33660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_327021902.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVSL
                     GRNDLLIRTFPRQESCLSTKYEKPRHPVGFFLLTI"
                     /name="hypothetical protein"
     gene            complement(435..1955)
                     /locus_tag="HV204_RS32990"
                     /old_locus_tag="HV204_32985"
     CDS             complement(435..1955)
                     /locus_tag="HV204_RS32990"
                     /old_locus_tag="HV204_32985"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_000076364.1"
                     /translation="MSQDPKKCPVTHLTTEAGAPVVDNQNSMTAGARGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYTRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDARKFPDLNKAVKRDPKTNKR
                     SATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTFSFINAQNERFWVKFHM
                     RTQQGIQNLTDAEAADLIAKDRESSQTDLFDAIERGEYPKWKMYVQIMPELEAETVPYH
                     PFDLTKVWPKGDYPLIEVGEFELNRNPENYFQDVEQAAFAPSNLVPGISYSPDRMLQAR
                     LVNYADAARYRVGVNHSQVPVNAARCPVNSNRRDGQGRMDGNYGSLPHYEPNSFNQWQE
                     QPQFKEPALKITGDADFWDFREDDNDYFSQPRALFNLMNDEQKQALFNNTAAAMGDALD
                     FIKYRHIRNCYACDPAYGQGVAKALGMTVADAQAARETDPAKGLPSFL"
                     /name="catalase"
     gene            2211..2420
                     /locus_tag="HV204_RS32995"
                     /old_locus_tag="HV204_32990"
     CDS             2211..2420
                     /locus_tag="HV204_RS32995"
                     /old_locus_tag="HV204_32990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181245240.1"
                     /translation="MAMQTGEWHRLSAGIRAESPVLSGLLQRRAVIRPLALRSFQLQRF
                     LVLRRPLLLPVRLNTVQPCDNRRS"
                     /name="hypothetical protein"
     gene            complement(2393..2608)
                     /locus_tag="HV204_RS33000"
                     /old_locus_tag="HV204_32995"
     CDS             complement(2393..2608)
                     /locus_tag="HV204_RS33000"
                     /old_locus_tag="HV204_32995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_071182205.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2612..3097)
                     /locus_tag="HV204_RS33005"
                     /old_locus_tag="HV204_33000"
     CDS             complement(2612..3097)
                     /locus_tag="HV204_RS33005"
                     /old_locus_tag="HV204_33000"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_071182206.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAAILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3132..3836)
                     /locus_tag="HV204_RS33665"
                     /old_locus_tag="HV204_33005"
                     /pseudo=""
     CDS             complement(<3132..3836)
                     /locus_tag="HV204_RS33665"
                     /old_locus_tag="HV204_33005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3775..4098)
                     /locus_tag="HV204_RS33015"
                     /old_locus_tag="HV204_33010"
     CDS             complement(3775..4098)
                     /locus_tag="HV204_RS33015"
                     /old_locus_tag="HV204_33010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181245241.1"
                     /translation="MLTMWVTEDEHRRLLERCDSKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAE
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4748..4861
                     /locus_tag="HV204_RS33020"
                     /old_locus_tag="HV204_33015"
     CDS             4748..4861
                     /locus_tag="HV204_RS33020"
                     /old_locus_tag="HV204_33015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            5169..5471
                     /locus_tag="HV204_RS33025"
                     /old_locus_tag="HV204_33020"
     CDS             5169..5471
                     /locus_tag="HV204_RS33025"
                     /old_locus_tag="HV204_33020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          complement(4214..4387)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          4311..4857
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.273"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2290..3785)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.786"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caacatcgaa tacatgaaag ccagcatccg tgccagggtg gagcacccgt ttcgcatcat
       61 caagcggcag ttcggcttcg tgaaagccag atacaaaggg ctgctgaaaa acgataacca
      121 actggcgatg ttattcaccc tggccaacct gtttcgggtg gaccaaatga tacgtcagtg
      181 ggagagatct cagtaaaaac cggaaataac gccagaaatg gtggaaaaaa tagcctaaat
      241 aggctgattc gatgtgtttg cgggaaaaaa atcggcccag atccgcgaaa ttttaatcag
      301 cgagtcagct tgggaagaaa tgacctgctt attcgcacct tccctagaca ggagtcatgc
      361 ttaagcacca aatacgaaaa accccgacat cctgtcgggt tttttttatt aactatctaa
      421 atcaaatttc cacattaaag aaaacttggt aaacctttag caggatctgt ttcacgtgcc
      481 gcttgagcat ctgctacagt catacctaag gccttagcaa cgccttgccc ataagctgga
      541 tcgcaagcat aacagttgcg gatatgacgg tatttgatga aatctagcgc atcccccatc
      601 gctgcggccg tgttattaaa caatgcttgt ttctgctcat cattcatcaa attgaacagg
      661 gcacggggct ggctgaagta gtcattgtca tcttcgcgga agtcccagaa atcagcatca
      721 ccggtaatct ttaatgctgg ttctttaaac tgaggttgtt cctgccactg gttaaaactg
      781 ttcggttcat aatgtggcaa tgaaccatag ttgccatcca tgcggccctg accatcacga
      841 cgattagagt ttacagggca gcgtgcagca ttgaccggaa cctgtgaatg atttacacca
      901 acacgataac gtgctgcatc agcatagttc accagacgtg cctgtaacat acgatccggt
      961 gagtagctaa ttcccggtac cagattgctt ggtgcaaagg cagcctgttc tacatcctgg
     1021 aaataatttt ccggattgcg gttcagttcg aattcaccga cttcaatcag tggataatca
     1081 cctttcggcc agactttggt caggtcaaat ggatgataag gaactgtttc agcttccagt
     1141 tccggcatga tctgaacgta cattttccat tttggatact caccacgctc aatggcatca
     1201 aataagtcag tctgactact ttcacgatct ttagcaatca aatctgctgc ttcagcatcg
     1261 gtcaggttct ggatgccttg ctgggtacgc atatggaatt tcacccagaa gcgttcattt
     1321 tgggcattga taaaactgaa ggtatggctg ccaaaaccat gcatatgacg gtagccagca
     1381 ggaataccgc gatccgacat cacaatcgtc acctgatgca aggcttcagg caataatgtc
     1441 cagaaatccc agttattggt ggcactgcgc ttgttggttt tcgggtcacg tttgactgct
     1501 ttattcaggt cagggaactt acgggcatcc cgcaggaaaa acaccggggt attattgccg
     1561 accagatccc agttgccttc ttcagtatag aatttcaggg caaaacctcg aatatcacgt
     1621 tctgcatcgg ccgcgccacg ttcacccgcc accgttgaga aacgcgcaaa catttcggtt
     1681 tttttgccga cttcagagaa aattttagcg cgagtatatt gggtaatatc attggtcacg
     1741 gtaaaggtac caaaagcacc tgaaccttta gcgtgcatac gacgctctgg aatcacttcg
     1801 cggacgaagt ttgctaattt ttcattcaac cataaatctt gggcaagtaa aggtccacgc
     1861 gcacctgccg tcatactgtt ctgattatcg accacagggg caccagcttc agtagtcagg
     1921 tgggtaacag gacatttttt agggtcttga ctcatactat taactcccgt ttcttttaat
     1981 ttaaatttcc tgcagaaatt taagattttc aatcctatct taccctttac ttgcttatta
     2041 aaatgttaaa ctaaggatat ttttgaaaat ttctttttat ttaacataag agttcttgcc
     2101 cgcaccgctt cgggcgggtt cagtgcgaaa gcggcgtcag cccggcaaaa caggcagaaa
     2161 actccccgct aaaccgcgca ttctgcgcca gatagatttc acctgtttgc atggctatgc
     2221 agacaggtga atggcaccgg ctttcggcgg ggatccgcgc cgaaagcccc gttttatccg
     2281 gtttgttaca gcgaagggcc gtcatacgtc cgctggcgct gcgatcgttc cagctccagc
     2341 gctttctggt gctgcgccgc ccgctgctgc tcccggtgcg cctcaatacc gttcagcctt
     2401 gcgacaaccg gcgcagctga tcgctcaaac tctgcacctg cctgctcaag cccgtgacgc
     2461 gctcgctcag cgccgcgttc tcccgttgca taagaccaga catcgtccgc cattccgcga
     2521 aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc
     2581 tcagcaactg tttttccagc tccgtcatgt gctatttccc cgccagtatc atccagctcg
     2641 aatcctctcc gaaccgtccc gcttccgggt tcaccttcac gcagcggcgt ctctgctcgc
     2701 cgcaggtcga gagctgcacg ccgctgttcc tctctgacag catggcctgt gtgctcttct
     2761 gctcccggat ggtcgtgtaa ttgtcgagta tctgctgact ctgccaccac agaatggccg
     2821 cactcgaggc gatcaacagc acggacacca ggacgatggt cagccacgtc tggctgacca
     2881 tgcgcagcat ccctttcgtg cgctggctca tggctgagga cagcttccgg tcgtggtcga
     2941 ggatggcggc gctgattctc ttctcgcttt cgctcagttc cgctctgaca gacttctcgt
     3001 gctcgctgaa tgcggctttc agcatctcgc cggtagtctg ctgctgcgct ttcgatttct
     3061 gctctaagtc cttcgccagc gttaaaagac tgttcataga tggctccctt cagtcggatg
     3121 tttcgccccc cgtccgggtc ggcaatgctg atgctgcttt tcgtggtgcg caccacctcg
     3181 aaacctgcgt tttccagcgc ctctgtgacg tcctgccgcg ttttcagctc cccggatgag
     3241 gcaagggcaa gcaggccgcg cgtaatcgcc tgggcggcct cctgcttcgc ttccggcagc
     3301 gcggaaggca tgaccagcgc ccgccggttt tccggcgcgt tcgggtcgtg cagccccagc
     3361 ctgccattaa ctatggtctg ccaggcatca atgcgcggac ggtcggcgcg gtcgtaatac
     3421 ggctggagac gtctgccggt cagcagctcc gtgttcggga tcaggaagtt cagctccagt
     3481 cgccctttat cccggtgctc cacccacagc acgctgtact ggtctttatc gagtccgggc
     3541 atcagaaccc gctcaaagcc cgccatcagc ctttcgcgct gcccgggcgg taaatccttt
     3601 tcggcaaagg acaggacgcc ggaggtgtac tttttggcgt acggcgaggc gtcgataagc
     3661 tcccggactt cctccggctt accctgcaga acgctggcgc cgtcacgctg ccggtctttc
     3721 cccagcaggt aatccaccgg accggcgcca ccgccgcgcc ctctgggatg aaatttaacg
     3781 atcatcctct gccccctttt caagcacagc gtgccgcagc cgttcgagtc cggcatcgat
     3841 ggccatcagc gcggcgacaa cctgcacgcg gtcgtgcccg ctgccgccac cggcgttaac
     3901 ctgtcgggca atctggttaa ggttgttacc catgcccgca agctggcgaa gcagcgccgg
     3961 cgagatcgag ggaagtttgc cggcgcgggc aggcttctcg tccaggcacg tctgccgcat
     4021 ccaggctgcg agctgcttac tgtcgcagcg ctccagcaga cgccggtgct catcctcggt
     4081 aacccacatc gtgagcattt tgctgcgttt gtctgccaat ccggcctcct gataacgccg
     4141 ggacgggcgg gaaatctttt cccggtcggc gcggcgttgg acaagccgca ggcgcgtcag
     4201 tggcttttag caggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
     4261 atactggctt actatgttgg cacggatgcg gaaatcagtg aagtgcttca tgtggaagga
     4321 gaaaaaaggc ggctccggcg cgtcagcaga atatgcgata caggatatat tccgcttcct
     4381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtgcgg gcttacgaat
     4441 ggggcggaaa tttcctggaa gatgccagga agaaacttaa cagggaaacg ataaggccgc
     4501 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     4561 aaatcagtgg tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc
     4621 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4681 ccgcgtttat ctcattccac gcctgacact cggttccggg aaggcagttc gctccaagct
     4741 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttttccg gtaactatca
     4801 acttgagtcc aacccggaaa gacacgacaa agcgccactg gcagcagcca ctggtaactg
     4861 atttagagga gttagtcttg aagttatgcg cctgttaagg ctaaactgaa agaacagatt
     4921 tggttcctgc gctcctcata agccagttac cacggttcaa agagttgctg ctctgagaac
     4981 cttcgaaaaa ccgccttgct gggcggtttt ttcgtttaca gagcaagaga ttacgacgct
     5041 acagaaagga tctcaagaag atccttcacc ataatggaaa tctatcccac ccgagcatat
     5101 aggatgcata tcgttataaa acatcggata ttatgcatcc caactaaaaa taagctcgag
     5161 gataaaaaat gagttcaatg gtttactgcc gaggatgcgg caaggaaata cacgaaacag
     5221 caaagtcatg cccacactgc ggagccacaa acgcctcttc aggttcaggt gaaaaaagta
     5281 gaatcgcagc cgcgctatta gcattcttcc ttggtggatt tggcgcacat aaattttact
     5341 taggtaaaat aggacaggga ttcctgtatc taattttttg ctggacattc atacccgcga
     5401 tcatcgcgtt tattgagttt attatttatc tatgcgattc cgatgaaaaa ttcgcaagaa
     5461 aatatggcta actcatagca aaaaagccct gcggggcttt ttttgtacca gtctgtgagg
     5521 cagatttacc ataacatccg ttatccgaaa tcttctttca taagttatat aaaattatat
     5581 acttaactat ttcacatcat ccaaaaattc accaaaacgc cgacatcgac acatctcggc
     5641 gtttttttaa gcgcttttgt catattccgc caataaccgg ttaaaaaatg atcaaaatta
     5701 tatcttttac tgacttctta agccttccat tttcgaattt atccccattt tctgcggaca
     5761 aatatcggga taattttttg ggtcgttcac tgtacgttct ggcttacagc catgcaggaa
     5821 attttccaat aggcagatga tcatcgattt cctattatgg actcatcaag aacaggatgt
     5881 tcgaagacct aaaacaataa tcataagtca aacaccagga tcgtgaagct agggaaggtg
     5941 cgaacaagtt cctgatatga gatcatcata ttcatccgga gcgcatccca gagggacatc
     6001 atgagccatc aactcacctt cgccgatagt gaattcagca ctaagcgccg tcagacccga
     6061 aaagagattt tcctctcccg catggagcag attctgccat ggcagaatat gaccgctgtc
     6121 atcgagccgt tttatcccaa ggcgggcaat ggccgacggc cctatccgct ggagaccatg
     6181 ctgcgtattc actgcatgca gcattggtac aacctgagcg acggtgccat ggaagatgcc
     6241 ctgtacgaaa tcgcctccat gcgcctgttt gcccgattat ccctggatag cgccctgccg
     6301 gatcgcacca ccatcatgaa tttccgccac ctgctcgagc agcatcaact ggcccgtcaa
     6361 ttgttcaaga ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac ccaaggcact
     6421 ttggtggatg ccaccatcat tgaggcaccc agctctacca agaacaaaga gcagcaacgc
     6481 gatccggaga tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac
     6541 attggtgtcg atgccaagag tggcctgacc cacagcctag tcaccaccgc ggccaacgag
     6601 catgacctca atcagctggg taatctgctt catggagagg agcaatttgt ctcagccgat
     6661 gccggctacc aaggagcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg
     6721 atcgccgagc gtcccggcag ggtaaaaacc ttgaagcagc atccgcgcaa gaacaaaacg
     6781 gccat
//
