LOCUS       NZ_CP056459             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00492 plasmid pRHBSTW-00492_5,
            complete sequence.
ACCESSION   NZ_CP056459
VERSION     NZ_CP056459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148635
            Assembly: GCF_013740655.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00492
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.620907128384x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013740655.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:49:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,830
            CDSs (total)                      :: 4,714
            Genes (coding)                    :: 4,618
            CDSs (with protein)               :: 4,618
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 55 of 96
            Pseudo Genes (internal stop)      :: 25 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056459.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00492"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00492_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1169..1387
                     /locus_tag="HV218_RS24045"
                     /old_locus_tag="HV218_24050"
     CDS             1169..1387
                     /locus_tag="HV218_RS24045"
                     /old_locus_tag="HV218_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1678..1863)
                     /locus_tag="HV218_RS24050"
                     /old_locus_tag="HV218_24055"
     CDS             complement(1678..1863)
                     /locus_tag="HV218_RS24050"
                     /old_locus_tag="HV218_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2229..2456)
                     /locus_tag="HV218_RS24055"
                     /old_locus_tag="HV218_24060"
     CDS             complement(2229..2456)
                     /locus_tag="HV218_RS24055"
                     /old_locus_tag="HV218_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1544..1631
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(377..905)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 catctaacta taggtcattt tttatgtgaa aagaggcttg atggtgggct gtatatcgag
       61 ttaggcgcga ggtgctatgg tcaaagtgtg gtgtcaggcg gggaggaggc ttatatgaga
      121 tgtaatccgg ccgatacata taccaagatg atttaagtat tcctctttgt gaggttggat
      181 gtctaattta taaaggatct tcttgagatc ctttttttcc gcgcgtaatc tcttgccctg
      241 taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt taggtaatcc tggcagatcc
      301 cctaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atttgtcctt tcagtgtagc
      361 ctcactaagg ccgccacttc aagaactctt gagacatctc tcgcacatcc tgtttgccaa
      421 tggccgttgc caatggcgat tagtcgtgtc tttccgggtt ggactcaagt tgatagttac
      481 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc atcctggagc
      541 gaactgcctt cccggaactg agtgtcaggc gtggaatgag aaaccgcggc cataacagcg
      601 gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag ccaccagggg
      661 gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg agcgtcagat
      721 tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttggcg cggccttatg
      781 ctttcttcgt taagtatctt cctggcatcc cccaggaaat ttctgatcca tccgtaagcc
      841 cgtcccgctc gccgcagccg aacgaccgag cggagcgagt cagtgagcga ggaagcggaa
      901 tatattctgt atcacatttt ctcctgacgc gttttctttc actttctgcg cctgtcttat
      961 gtggcattaa tgctatgtgt tactgccatg ctacatctta agccagtata cactccgcta
     1021 gtgctccgtg actggtccgg cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt
     1081 tccggcctga ctgcaatttt ttttttttca tccctgcccg ctaccctgta aacctttctt
     1141 ctgcgttgcc gttaacagta ggtaagccat gtttggtttt tcttccggag ccctgatgac
     1201 tcttagtgat gatgagcgac acctgcttgt gtccgttgtt tcggtctggc tacggcgtgc
     1261 cggaggggat gccggagcca tgatgctgga tgcataccgg cagattttgt ctgaaaccga
     1321 accggccgta aggactgtca tgctcgaatt tcttgaatcc gttcgcattc attacatttc
     1381 gtcctgagca ttttctgtat ttctgtccat acttaaacga ggcggccgtc acggacggtg
     1441 gtcgtcgttc catgatgctg ttatggacga gtgagagtaa tctaatctaa cttgattgag
     1501 ttccgggcgt gccgcccggt tttttttgct gtttcccggt gtccgttccg ggacaggatg
     1561 tgcatttgta ataccgcgca cgcgccggca tcacaaatac cctggtcagg gctgcgcccc
     1621 gacaccccgc catcgcctca cggccgcgca ctgaattgcg cacccgttca ccgattttca
     1681 cgcattttcc tcgcctatct gtgcgttcag catggcgtaa agcgcttcgg cctgatcgtg
     1741 caggtgctca cagcagccgg cagcctcatc cagatccatc tgctcgagcc gctcaaggag
     1801 gagaagtgtc tgtgcgcgga taaatttggc catattgagg gcggcggatt cctgtctggt
     1861 cactggtgat tttccccgtc tggtgatgat ttttgcgtga aaagagcatg gcgtcattct
     1921 ggtgcatagt catgcatgcc gttaaaattt aacaggcgcg tttctgacgg gttccggggt
     1981 ggtttgttgt ggtttttgtc atggtttcgt caggaacgcg ctgaacgcgt ctcaggcggt
     2041 gcgggcaacg gatgttatgg taaattcttg ggggcttgat tataaaatac cgcacttcct
     2101 cttatgtctt gtgcttttta gacaaccctg aaagctttta gctttggtga tgcaatttgc
     2161 tgggattttg gcggagaacc acaggtaaag aaaaaggcca cattagcggc ctttttcgga
     2221 gaagatgctc agtgcggaat ttcggagact tctttaacgt cggttttatt gatctgctgt
     2281 ttgacaccat tcgcgtctgt atagccaagc aggcctgagt ctgattcttt aggtttgccg
     2341 tcgctgacaa tagtgcgacc gtcattggtg tgtatggcat agcttgtgcg cgtgcagccg
     2401 gtgagggctg aaagcgcaac tgccgctgcg aacactgaaa caaaaatctt tttcaaagcc
     2461 agctcctttt atccatatca gttttggtca ctaac
//
