LOCUS       NZ_CP056458             2746 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00492 plasmid pRHBSTW-00492_4,
            complete sequence.
ACCESSION   NZ_CP056458
VERSION     NZ_CP056458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148635
            Assembly: GCF_013740655.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00492
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.620907128384x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013740655.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:49:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,830
            CDSs (total)                      :: 4,714
            Genes (coding)                    :: 4,618
            CDSs (with protein)               :: 4,618
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 55 of 96
            Pseudo Genes (internal stop)      :: 25 of 96
            Pseudo Genes (multiple problems)  :: 14 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00492"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00492_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2515..2746,1..143)
                     /locus_tag="HV218_RS24025"
                     /old_locus_tag="HV218_24030"
     CDS             join(2515..2746,1..143)
                     /locus_tag="HV218_RS24025"
                     /old_locus_tag="HV218_24030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            complement(332..>433)
                     /locus_tag="HV218_RS24030"
                     /old_locus_tag="HV218_24035"
                     /pseudo=""
     CDS             complement(332..>433)
                     /locus_tag="HV218_RS24030"
                     /old_locus_tag="HV218_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            1723..1950
                     /locus_tag="HV218_RS24035"
                     /old_locus_tag="HV218_24040"
     CDS             1723..1950
                     /locus_tag="HV218_RS24035"
                     /old_locus_tag="HV218_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(1998..2186)
                     /locus_tag="HV218_RS24040"
                     /old_locus_tag="HV218_24045"
     CDS             complement(1998..2186)
                     /locus_tag="HV218_RS24040"
                     /old_locus_tag="HV218_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(755..1250)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.171"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 ccaaagacgg gcttaaagtt agcaacatga agaaggtatt gatcgaggca gggagcgctg
       61 tagggatgga tgataaagag ctaaaccctg actttacgac ctatgaaata ggccgttttc
      121 tcttcaaaca tcttcaggga taaaggtgtc taccagttcg tggccgggat tttaggttca
      181 attcagctag ttcccggaca gaaaaaaagc caccgtgtag gtggcttttt tattcctgct
      241 ttaggcaaag cattattgat agcatttgca agcaaaccgt tagcgacttc tgctgaatgc
      301 ttctgggcgg attcaagttc gaagtgcaac gctaagccgc ctgtcttagc tcagcaaaga
      361 ccactgaagg ttgccggaat ccaagacatt tccgtggcct gctattcagt gctctctggt
      421 acttgcggag ctcccctgtg tgataagtca ggtgtggaat ttttacgtat aaggccccgc
      481 taggcggaaa ccaatactga agccaaatca ctggtaaaca gccggatctt cttgtgatcc
      541 ttttactctc gtcgtaatct catgtcctgt aaacaaaaaa accaccgcta ccaacggtgg
      601 ttcgtttgcc ggaatcaaga gctaccaact ctttcttccg aaggtaactg gctttcacgg
      661 agcgcagata ccaaaactgt ccttccagtg tagccgcagt caggccgcca cttcaagaac
      721 tctgtagaag cactctacta ccctgctccg tgaatcctct taccaatggc tgctgccaat
      781 ggcgttaagt cgtgtctttc cgggttggac tcaagacgat agttaccgga caaggcgcag
      841 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
      901 gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt
      961 aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta
     1021 tctttaagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     1081 tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttcttgct tccctgttaa
     1141 gcttcttcct ggaagatgcc aggaaatttc cgccccgcca gtaagccggt accgctcgcc
     1201 gcagccgaac gaccgagcgg agcgagtcag tgagcgagga agcggaatat ctccgggctt
     1261 atctttggca ccgtacgcgc catagctgca ttttttaata cgatgcagga tagggcaaac
     1321 gccgcaaaat gacgtcatcc gacatcattc cgccgcgttc gtccagtgac ctacagtcgg
     1381 ctctggggtg tcggggctgt gccctgacca ggcgttttgg agtatcgcca ccgtggtggc
     1441 ggtgctacaa aacatatcct gtcccttcct tggcatctct gaagtttagc cagtatacac
     1501 tccgctagcg ctacgtgact ggttcagggc tgcgccccga cacccgctaa aagcactgac
     1561 gcgcctgcgg cttgcccaac cccgcgccgt ccgggaaaga atttcccgat cgacccgacg
     1621 tttctgttta cctgtgatgt taacgtttgg atctttttcc cgggtttacc tattttcttc
     1681 taatgaagcg ttcacagctg acgttaatag atgggtgctt tcatgtttgg tttttcacca
     1741 ggagaactta tgaacctttg ccccgatgaa cgtctgctct ttgtgcggat gatttccgct
     1801 atgcttcgcc gttcgggcgg tgatgcgggt gctgttatgt ttgaggcgta tcgccatatt
     1861 gtgtccgata ccaatcaagc tcgtcgttcc tgcatgctcg acctgctcga aagcgtcagg
     1921 catgactatg tacacggtgg atatacatag ttgccctgga tacgcttttc gtctaagtga
     1981 gcgttctttt cgtctcttta ttcgccgtcc tgtataccga agcgtgcgct cagcttctga
     2041 taaagcgctt ctgcctgttc gtgcattcgc tcacagtcgg ctgcctcgtc atcaagatcg
     2101 agcacgtcga gtttttcgag cagcagcagc gactgcgccc ggataaattt tgccatgttc
     2161 agtgcggctg attcctgttt tgtcatacgg ctttttcccg gttttatggt gatcgcagac
     2221 tgaaatctta cttcattcac ttgagtgcat agccatgcag gtacgcagaa atcatcagcg
     2281 cgtttctgag cggttttcag ggggtaagat gtgttctttt gcaggtgagg tcgtagaaac
     2341 gccctgagag cctctcaggc ggtgcacgca acggatgtta tgttcaatct ggcggtgcgg
     2401 catagttatc atcgcaccta cagaccgctt gttgtattca gaacaatatt tatttgtata
     2461 atttgtattt tgttgtatta tttcttcaaa ttagagcggt ggggtaataa taatatgaat
     2521 ggtatgtctg acgtaagact aaagaaatca actattgaga gcctaaaaga gtacgtgaat
     2581 gactttggca tgacggttga tgatgttgtc ctgaacttaa ttgaagagaa taattcatta
     2641 aaatcgttat taggtcaggg cttttctttg cctggtggag ataaggacac acattccttc
     2701 aaggattacg tgattcataa gcttccaaaa ggaaagatct tggtta
//
