LOCUS       NZ_CP056440             1917 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00510 plasmid pRHBSTW-00510_9,
            complete sequence.
ACCESSION   NZ_CP056440
VERSION     NZ_CP056440.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148642
            Assembly: GCF_013736155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00510
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.0175829502872x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013736155.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:17:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,021
            CDSs (total)                      :: 5,891
            Genes (coding)                    :: 5,697
            CDSs (with protein)               :: 5,697
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 77 of 194
            Pseudo Genes (incomplete)         :: 124 of 194
            Pseudo Genes (internal stop)      :: 35 of 194
            Pseudo Genes (multiple problems)  :: 31 of 194
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056440.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00510"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00510_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            819..1121
                     /locus_tag="HV225_RS29950"
                     /old_locus_tag="HV225_29955"
     CDS             819..1121
                     /locus_tag="HV225_RS29950"
                     /old_locus_tag="HV225_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_181352640.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIVAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1381..1617)
                     /locus_tag="HV225_RS29955"
                     /old_locus_tag="HV225_29960"
     CDS             complement(1381..1617)
                     /locus_tag="HV225_RS29955"
                     /old_locus_tag="HV225_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1783..1917)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.23e-64"
     Typing          18..509
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ggatatgtag tacaggatat attccgcttc ctcgctcact gactcgctgc gctcggtcgt
       61 tcggctgcgg cgagcggtgc cggcttacag gcggggcgga aatttcctgg gagataccag
      121 gaaggagctt aacagggaag agataaggcc gcggcgaagc cgtttttcca taggctccgc
      181 ccccctgaca aacatcatga aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg
      241 cttaaagata ccaggcgttt ccccctggtg gctccctcgt gctcccccgc attcctgcct
      301 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca
      361 ctcagttccg cgaaggcagt tcgctcctgg atggactgta tgcacgaacc ccccgttcag
      421 tccgactgct gcgccttatc cggtaactat catcttgagt ccaacccgga aagacacgac
      481 ttatcgccac tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg
      541 cgcctgttaa ggctaaactg aaagaacaga ttttgttact gcgctcctcg taagccagtt
      601 accacggttc aaagagttgc tgctctgaga accttcgaaa aaccgccttg cagggcggtt
      661 ttttcgttta cagagcaaga gattacgacg ccacagaaag gatctcaaga agatccttca
      721 ccgtaatgga aatctatcca atccgagcac ataggataca catcgttata aaacatcgga
      781 tattatgatc ccaattaaaa taagctcgag gataaaaaat gagttcaatg gtttactgcc
      841 gaggatgtgg caaggaaata cacgaaacag caaagtcatg cccgcactgc ggagccacaa
      901 acgcctcttc aggttcaggt gaaaaaagta gaatcgtagc cgcgctatta gcattcttcc
      961 ttggtggatt tggcgcacat aaattttact taggaaaaat aggacagggg ttcctgtatc
     1021 taattttttg ctggacattc atacccgcga tcatcgcgtt tattgagttt attatttatc
     1081 tatgcgattc cgatgaaaaa ttcgcacgaa aatatggcta actcatagca aaaaaaagcc
     1141 ccctaagggc ttttttttgt acctgacggt cagaccgatt aaacataaca tcccttgccc
     1201 gcacccccgc aggcgccctc agcgcaatcc tgctgcatca gggccgtttc cagcaccttt
     1261 cctcaccagt atcccggaaa aacgcccctg atgatttctg cgcgactgca tggctatgca
     1321 cgcaggtgaa tggcaggtga tttcgcagcg tcagcaccac taaaccgcca aaagccaaaa
     1381 tcagcggcgc ggcgcgcgcg gacgtgacca ggcaaaatcg gactgctgcc gctcatcctc
     1441 acgtaatgca cgctggcggt cggcgtaacg ctgcaactca atttgcgcac gcgctctgtt
     1501 caggacgatg tgccggttag gctgatttag ctttttacct gacgctcttt cagctgcggc
     1561 aacagcatca tcaagaaatt ttttctctgc aggcgtcaag tttgtcagtc tggccataac
     1621 gttctccagt cagaataaat cagcacagtc tgccagacac agtagaaacg atgaagcgcc
     1681 agcgacggtg aaaggcagtc agaaacggtg aacgggagcg gattttaatc cggtgccgtg
     1741 aggcgttgga caagccgcag gcgcgtcatt agcttttagc gggtttcggg gcgcagccct
     1801 gaaccagtca cgtagcgcta gcggagtgta tactggctga ctatgttggc acggatgtgg
     1861 aaatcagtga agtgcttcat gtagcaggag aaaaaaggcg gcaccagcgc gtcagca
//
