LOCUS       NZ_CP056439             3514 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00510 plasmid pRHBSTW-00510_8,
            complete sequence.
ACCESSION   NZ_CP056439
VERSION     NZ_CP056439.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148642
            Assembly: GCF_013736155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00510
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.0175829502872x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013736155.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:17:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,021
            CDSs (total)                      :: 5,891
            Genes (coding)                    :: 5,697
            CDSs (with protein)               :: 5,697
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 77 of 194
            Pseudo Genes (incomplete)         :: 124 of 194
            Pseudo Genes (internal stop)      :: 35 of 194
            Pseudo Genes (multiple problems)  :: 31 of 194
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00510"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00510_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3255..3514,1..196)
                     /locus_tag="HV225_RS29925"
                     /old_locus_tag="HV225_29930"
     CDS             join(3255..3514,1..196)
                     /locus_tag="HV225_RS29925"
                     /old_locus_tag="HV225_29930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_249415993.1"
                     /translation="MTGSGLRPETPLTRLRLVQPRADRERLSRPSRRYQEAILADKRNK
                     MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSISPALLRQLAGMGNN
                     LNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            405..>833
                     /locus_tag="HV225_RS30385"
                     /old_locus_tag="HV225_29935"
                     /pseudo=""
     CDS             405..>833
                     /locus_tag="HV225_RS30385"
                     /old_locus_tag="HV225_29935"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            874..1371
                     /locus_tag="HV225_RS29935"
                     /old_locus_tag="HV225_29940"
     CDS             874..1371
                     /locus_tag="HV225_RS29935"
                     /old_locus_tag="HV225_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1378..1593
                     /locus_tag="HV225_RS29940"
                     /old_locus_tag="HV225_29945"
     CDS             1378..1593
                     /locus_tag="HV225_RS29940"
                     /old_locus_tag="HV225_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2617..2748)
                     /locus_tag="HV225_RS30520"
                     /old_locus_tag="HV225_29950"
     CDS             complement(2617..2748)
                     /locus_tag="HV225_RS30520"
                     /old_locus_tag="HV225_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072072138.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRISRAS"
                     /name="replication initiation protein"
     Typing          3111..3284
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2636..3144)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.706"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          187..1709
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3391..3512
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="98.0"
                     /evalue="3.06e-19"
ORIGIN
        1 tccctcgatt tcgcccgcgc tgctgcgtca gcttgccggc atgggcaata acctgaacca
       61 gatcgcccgg aaagtgaacg ccggtggtgc cggtcatgac cgggtgcaga tcgtggcagc
      121 gctgatggcg atcgatgccg gtctggaacg gttgcggcat gccgtactgg aaaaagggcc
      181 ggacgatgat tgttaaattc catccccggg gtcgcggcgg gggcgccggt cctgtggatt
      241 atcttctggg taaagaccgg cagcgtgaag gtgccagcgt cctgcagggg aagccggaag
      301 aagtccggga gctgattgat gcgtcgccgt atgtgaaaaa atatacctcc ggggtgctgt
      361 cattcgcgga ggcggacctg ccgccgggtc agcgtgaaaa actgatggcc agctttgagc
      421 gggttctgat gcccggactc gataaagacc agtacagcat tctgtgggtg gaacacgcag
      481 acaagggtcg tcttgagctg aattttctga tccccaatac cgaattactg acggggaaac
      541 gcctccagcc gtattacgat cgtgccgacc ggccgcgcat cgatgcgtgg cagacagtgg
      601 tcaacggcag gctggggcta catgatccga acgacccgga gaaccggcgg gcgctggtca
      661 cgccttcagg actgccagaa acgaagcagg aagccgcaga gacgattaca cggggtttgc
      721 tgatgttagc ctcatcagga gagctgaaaa cgcgtgatga cgtcacaggg gcgctcacag
      781 cggccggttt tgaggtggtc agaaccacaa aaaacagtat cagcattgcc gacccggacg
      841 gggggcgaaa tatccgactg aggggagcca tttatgagca gtcttttaac ggcctcgagg
      901 gacttcgagg agaaatcgaa agcacagcag cagcttaccg aagagaggct gaaagccgca
      961 ttcagcaagc acgagaacgt tgtcaaatcg gaactgaacg caagcgcgaa gagaatcaac
     1021 gacgccatca gcgcccacga acaggggatg aacgcggcga tgcagtcgaa ccggctgagc
     1081 gtgctgcgaa tggtcgggcg gacgtggctg accatcacca tggtgtcagg gctgctgttc
     1141 gccagcctga gcggggtgct gtggtaccag gggaaccgga tagcggcgaa cctggcggaa
     1201 atcaggcagc agagagatac gctgtcgaaa ctgcacatgc agacgtgggg cgtgacctat
     1261 ctggagaacc gcaacggacg gtttctggtg ctgccggagg ggatgaaagc cgaaacgggc
     1321 cggacggtgg acaacaagac gcggaacgcg gtgaagctgg tacgggagta gagcagaatg
     1381 acggaactgg aaaaacaatt gctgagcgcg ttagagcagc tacagcagga ctactcgaga
     1441 aggctggacg agtgggagag cgccttcgag gaatggcgga agatgtctgg tcttatgcaa
     1501 cgggagaacg cggcgctgag cgagcgcgtg tcgggcttga gcaggcaggt gcagagtttg
     1561 agcgagcagc tgcgccggtt atcgacaggc tgaatgagat tgagcaacag cgatacgggc
     1621 aaacaacgga aaaggaacca gagaagacag cagatctggt accggaacgg caacgcacat
     1681 tatctcatgg tgacagggag tatggagact gataaacgcc cgtctcaggt ggcctgagcg
     1741 tgacggggat catggccatt ttctgcgatt tcagcacaca gagagtcttt tttaaccgaa
     1801 atcaccggct atgcagtgat gtgaataagt gatgcatatg cgtaaaataa atcaggtgcg
     1861 tttctggtgg ttttcggtgt acttgccacc accttttgcc cgtcagccat cagaaacgcc
     1921 ctgagtgcct ccggcgcggt gcgggtaaga agacgttatg gtaaatcggt ggctttattt
     1981 cgatccttca tgcttcaggg gctgtatcac tttgttagtt ttagttttgt ggtttcggct
     2041 tggattccga gaagatcgtg gaggttattg ttgatagcaa attttatatc ttcaaaactt
     2101 gcatcaatca tgagtccctc atccacacct cgtaaaaaaa tttttgtttt tgagttacca
     2161 catgatttta ttaagataat ttcatttttt ttaacatgac attgtcattg tttgttgtcg
     2221 tcagatgaat cattatcgtt gtttttgtgt gtgtttgtta aggctgctaa gattattcct
     2281 aaccactgat tttgtacata tcatcaaaaa aataaaaaaa ctaatgccct gtggtgggta
     2341 tgtaacaaat cacggggagc gggtcagaac tacgccaaaa ctttgaatga tcgggtggca
     2401 ggctaaatga aatcttgaga ttcattcagc ctcgtcgtaa tctcttgctc tgtaaacgaa
     2461 aaaaccgcct tgcagggcgg tttttcgaag gttaagtcag ttggggaact gcttaaccag
     2521 gtaactggct tcagcagagc gcagatacca aatactgtcc ttccagtgta gccgtagtta
     2581 ggccaccact tcaagaactc tgtagcatca cctacattaa ctcgctctgc taatcctgtt
     2641 accagtggct gctgccagtg gcgctttggc gtgtctttcc gggttggact caagacgata
     2701 gttaccggat aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt
     2761 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata
     2821 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     2881 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     2941 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgcgg
     3001 ccctatcgct tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     3061 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     3121 agcggaatat atcctgtatc acatattctg ctgacgcgcc ggtgccgcct tttttctcct
     3181 gccacatgaa gcacttcact gacatcctca tccgtgccaa catagtaagc cagtatacac
     3241 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aaccccactg acgcgcctgc
     3301 ggcttgtcca accccgcgcc gaccgggaaa ggctttcccg tccgtcacgg cgttatcagg
     3361 aggcgattct ggcagataaa cgcaacaaga tgctcacgat gtgggtgacg gaagacgaac
     3421 accggcggtt gcttgagcgg tgcgacggaa aacaactggc ggcgtggatg cgccagacct
     3481 gccttgatga gaaaccgaca cgcgccggca ggct
//
