LOCUS       NZ_CP056431             2836 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00515 plasmid pRHBSTW-00515_7,
            complete sequence.
ACCESSION   NZ_CP056431
VERSION     NZ_CP056431.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148644
            Assembly: GCF_013735895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00515
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.2829444239567x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,893
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 71 of 170
            Pseudo Genes (incomplete)         :: 106 of 170
            Pseudo Genes (internal stop)      :: 28 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00515"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00515_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2588..2836,1..78))
                     /locus_tag="HV227_RS29245"
                     /old_locus_tag="HV227_29250"
     CDS             complement(join(2588..2836,1..78))
                     /locus_tag="HV227_RS29245"
                     /old_locus_tag="HV227_29250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064165621.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFH
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(462..599)
                     /locus_tag="HV227_RS29635"
     CDS             complement(462..599)
                     /locus_tag="HV227_RS29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074189340.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPDGHAKTPLAAAIGNWIYTRERY
                     "
                     /name="replication initiation protein"
     gene            complement(1601..1996)
                     /locus_tag="HV227_RS29250"
                     /old_locus_tag="HV227_29255"
     CDS             complement(1601..1996)
                     /locus_tag="HV227_RS29250"
                     /old_locus_tag="HV227_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228725605.1"
                     /translation="MKERIVKCVTPRFSTMHWLIAFLAFIFPGRSFADGYTAYYRCNGT
                     QEASLTVKDNKLSMVVGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDI
                     NNMTLLVKNNGYIIVKTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2017..2562)
                     /locus_tag="HV227_RS29255"
                     /old_locus_tag="HV227_29260"
     CDS             complement(2017..2562)
                     /locus_tag="HV227_RS29255"
                     /old_locus_tag="HV227_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064165620.1"
                     /translation="MFFKRYLTEKEIYKTALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLRITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          985..1165
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.246"
                     /coverage="83.0"
                     /evalue="4.54e-45"
     Typing          complement(491..892)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.931"
                     /coverage="90.0"
                     /evalue="5.15e-127"
ORIGIN
        1 cattttggtt ttattcgaaa gccgttgtaa ggattgcttt aatcctatat ctagcatgat
       61 atttgttctt tctctcatta cacccccccc tcactattgc gttttttgtt gcgcgtaaag
      121 ctaccattgc gcattggaaa acgcaataaa aaaggagtga gggagtggtt tgtacatgat
      181 gtgaagtgcg ctgagggcat cagtggcggg tgtttttccg tgggatggat ggagctccgg
      241 ggataacaga actgacgatc atttaaagga tcttcttgag atcctttttt tctgcgcgta
      301 atctcctgca ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc
      361 agttggggaa ctgcttaacc gggtaactgg gtatacgaga ccgcagatac caaaactgtt
      421 ctttcagtgt agccgcagtt aggcacccac ttcaagacct ctcaatatct ctctctcgta
      481 tatatccagt taccaatggc tgctgccagt ggcgtttttg cgtgcccgtc cgggttggac
      541 tcaagacgat agttaccgga aagggcgcag cagtcgggct gaacgggggg ttcgtgcata
      601 cagcccagct tggagcgaac gacctaaacc gagccgagat accagcgtgt gagctatgag
      661 aaagcgccac acttcccgaa cgggagaaag gcggaacagg tatccggtaa gcggcagggt
      721 cggaacagga gagcgcacga gggagccgcc ggagagaaat caccggcatc tttaagtcct
      781 gtcgggtttc gccctcctct gacttgagca ccggaaatca tctgattttt gtgatgctcg
      841 tcaggggagg cggagcctat ggaaaaacgg caacgccgca tcctttttcc ggtccctcct
      901 gattttagcg ccatcacttt atcccgttat ccgtgagcag gtaacgcccg ccgcagccga
      961 acggcaggag cgtgagcgac cgagtgagcg aggaggcgtc atttcatcgt cactggcatg
     1021 tcgcactgac gcgtctcagc cgcatatttt ctcctgctac atgaagcact ttcctgaatc
     1081 cccacttcga tttggcatat taagccagta tacactccgc tagcgctgcg tgactggttc
     1141 agggctgcgc cccgaaaccc gctaaggtca acttcgccgg gccacggcct gctcgtttct
     1201 ggtactgcca tcccgatggc ggtactccag aaacacatct tgtccggctg tttttgtttc
     1261 gatgcaggat agggcgaacg ccgcaaagtg acgtcgcttt ctgacgcgct gcgcttgtcc
     1321 aacgcctcac ggccacacaa taaattgcgt ccccgttcac cgttttctgt tgggtttgat
     1381 gccggtttta agcgatatgg ccagaattca ggacggaaat gctcgtcagt cagtcaggtg
     1441 catggttatg cacggggctg aaaatcactg tatgcgattc tgagcggttt tcggtgagaa
     1501 gacggggttt tttatgtctc agggcgtaaa atcgctctga gggcttctga ggcggtgccg
     1561 acaacgggtg ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag
     1621 ttaaccagca ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat
     1681 tgttaatgtc aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc
     1741 tctgcattac tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa
     1801 acttagaaag acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct
     1861 gagtaccatt acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata
     1921 taaatgctag gaacgcaatt aaccaatgca tagttgagaa tcttggtgtt acacatttaa
     1981 ctatcctctc tttcattttt cgttcaccat acattgttat ctgattttta atttttctat
     2041 tttataaacc aagtccctac caaaaaggcg aaaattatcc tcaaataata ataattgttt
     2101 aagaacatcc atgaaagcag ctgcttcccc ctcagttatg ggagctactc tatataacat
     2161 ataacctgag ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg
     2221 attatcattt ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt
     2281 acgccctatt gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc
     2341 tttgtttatg cctgcttgag tgattctcag agactctaca ccgtgtatgt tgtatgagat
     2401 aaaatgtaat aaattaagat gtaatgcaat taatccattt ttgtttacat ctgagtattt
     2461 ggataaaaaa cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgt
     2521 cttgtatatt tccttctcag ttaaataccg tttaaaaaac ataattctcc gcctccgtat
     2581 agcgtgccta ttttatttcg tcgtgaaact tagctctgaa atattccttt aaaaccatca
     2641 tgtcattaat aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag
     2701 ggagcggtaa ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc
     2761 attcaggctt ttcgccaatg cgctcttcaa ctgaaagtga acctgataat ctctggttaa
     2821 taagatcact tagact
//
