LOCUS       NZ_CP056430             3733 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00515 plasmid pRHBSTW-00515_6,
            complete sequence.
ACCESSION   NZ_CP056430
VERSION     NZ_CP056430.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148644
            Assembly: GCF_013735895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00515
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.2829444239567x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,893
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 71 of 170
            Pseudo Genes (incomplete)         :: 106 of 170
            Pseudo Genes (internal stop)      :: 28 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056430.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00515"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00515_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3334..3733,1..1016))
                     /locus_tag="HV227_RS29220"
                     /old_locus_tag="HV227_29225"
     CDS             complement(join(3334..3733,1..1016))
                     /locus_tag="HV227_RS29220"
                     /old_locus_tag="HV227_29225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181486824.1"
                     /translation="MIHKQIGGKKRQKNVEHSLNYILRVRPEDTAEDRNFVQVMTTTTR
                     ADIENFNDYIKPKKFSHPYITGVLSFEESDTDENLKQKMIADFEEVLFSGIALENRPPV
                     LWVQHRDKGRLELNYTTFNALQDGRAFKAYYHNTDHHLFNAFCEKNNFEHGFSSVLDKD
                     EKNSLIRVPTNIPEEKRAKLEQIQNEILGKIIVQEINNRDELIDYLKSKDIHINRVRKN
                     QISIKFSPDEKPTVLKGDIYEEDRDYRIYREPPKNNRERDPEFAKRALAEHRKIFDRLL
                     KNRSGRVAERYNRPPKKNASIIEPRAKKTNDHEDQNNQHKNTDSFDLDSFSKFVFNDFD
                     NNKNVSEVLNEQNNRDDKTEPREEKSQREERTRQIEAIDFEQQRINKIAETVRDRQFRN
                     RAEANTIEFRIGFTRRHFSGFVQFFSKIIRPIFVNTRKNEVRSTIEERRKLAQQVIKNQ
                     KQKEAKRERLKYK"
                     /name="hypothetical protein"
     gene            complement(991..1365)
                     /gene="mobC"
                     /locus_tag="HV227_RS29225"
                     /old_locus_tag="HV227_29230"
     CDS             complement(991..1365)
                     /gene="mobC"
                     /locus_tag="HV227_RS29225"
                     /old_locus_tag="HV227_29230"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_181486825.1"
                     /translation="MRDYKEKYQRKNFVSLCLSDQEMSEVDELTKRLDLTRAAAIRELL
                     LLSTTKLKNKIHKNDNKEQLFLLSNIANNINQVAKKMNTDINYFLSGNGEQFAYLFDKI
                     VDDIEKIKRGSTNDTQTDRR"
                     /name="mobC"
     gene            1929..2795
                     /locus_tag="HV227_RS29230"
                     /old_locus_tag="HV227_29235"
     CDS             1929..2795
                     /locus_tag="HV227_RS29230"
                     /old_locus_tag="HV227_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_181486823.1"
                     /translation="MFNNDNQQNFVSQMPVKPYCSDDLSTGLRIRKKEKALEMLYIQAN
                     QPAIQTCLLFDLDDDNCFYKFEDVGLPVPHFITKSPNSGRCHYGYILQNGVCKTQQARL
                     KPLRYAAAIENGMANKLGSDPGYVGLITKNPLNPHWSPYWSGADLYDLDYLADFVDLNA
                     PNPKEKRGQEYGLGRNVNLFDDLRLYAYRHVLEFKDSSSFDSWMREIENQAINLNTLCN
                     PKNLLPFGEVKATARSIGKWTWKNFSRSAFSSVQAHRGRMNGGKKKNNYKKQSNIEEMI
                     SFLGGEK"
                     /name="replication initiation protein"
     gene            2792..3052
                     /locus_tag="HV227_RS29235"
                     /old_locus_tag="HV227_29240"
     CDS             2792..3052
                     /locus_tag="HV227_RS29235"
                     /old_locus_tag="HV227_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase family protein"
                     /protein_id="WP_064645480.1"
                     /translation="MRVSRNGLTQKQLAEKFNISVTTVIKYTAIEREEYESKAKERRLK
                     AYTMRSNGFSLKDIALELGCSYNAAAALVKRYKQLDNKGTI"
                     /name="DDE transposase family protein"
     gene            3049..3228
                     /locus_tag="HV227_RS29240"
                     /old_locus_tag="HV227_29245"
     CDS             3049..3228
                     /locus_tag="HV227_RS29240"
                     /old_locus_tag="HV227_29245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064645481.1"
                     /translation="MKDGFAGILAVAFYVAGFVGWLTSVISDASHSRIGWVIADILVFP
                     MGVVRGVGMWFGFF"
                     /name="hypothetical protein"
ORIGIN
        1 aaatcgttaa aaacaaattt agagaaacta tcgagatcaa agctgtctgt atttttatgt
       61 tgattgttct gatcttcatg gtcatttgtt tttttagctc ttggctcaat aatactcgca
      121 ttttttttcg gtggtctgtt atatctttca gcaactcttc ctgatctgtt tttaagtaat
      181 ctatcaaaga tttttctatg ttctgcgagt gctcttttag caaactctgg atctctttct
      241 cggttatttt ttggtggttc tctataaatt ctgtaatctc tgtcctcttc ataaatgtct
      301 ccttttaaca ctgttggctt ttcatctggt gaaaacttaa tagatatctg attttttcta
      361 actctgttga tgtgaatatc ttttgatttt aaataatcga tcaactcatc tctgttgttt
      421 atttcttgaa caataatttt tccgaggatc tcgttctgta tctgttcaag ttttgctcgt
      481 ttttcttccg ggatgttggt cggaaccctg atcaagctgt tcttttcgtc tttgtccaac
      541 actgacgaaa agccgtgctc aaagttgttc ttctcacaaa acgcattgaa aagatgatga
      601 tctgtattgt ggtagtaggc tttaaaggct ctgccgtctt gtaaggcatt aaaggttgta
      661 tagttaagct ctaaccttcc cttatctctg tgttgaaccc agaggactgg agggcgattt
      721 tctagggcaa tacctgaaaa aagaacctct tcaaaatctg cgatcatttt ctgctttaaa
      781 ttttcatctg tgtcgctttc ctcaaacgac agaaccccgg taatatatgg gtgagaaaat
      841 tttttaggct ttatataatc attaaaattt tcaatatctg ctcttgttgt ggttgtcata
      901 acctgaacga aattgcgatc ttctgctgtg tcttctggtc taactcttaa aatataatta
      961 agcgaatgct caacattctt ctgtcttttt ttacctccta tctgtttgtg tatcattggt
     1021 agaccctctt tttatttttt cgatatcgtc aacaatcttg tcaaacaaat atgcgaactg
     1081 ttctccgttt ccacttaaaa aataattaat atcagtattc attttcttag caacctgatt
     1141 aatgttattc gcaatgttag atagcaaaaa caattgttct ttattatcgt ttttgtggat
     1201 tttgtttttt agttttgttg tgctcaacaa aagcaattca cgaatggcag cagctcttgt
     1261 taaatctaat cttttagtta attcgtcaac ttctgacatt tcttgatctg aaagacaaag
     1321 agaaacaaaa tttttcctct ggtatttttc tttataatca cgcatatcct gtccttttct
     1381 tttagctttt tgattttgac cttaaggggg attgaagggg gcgaagccac ctcaccagta
     1441 aacaatacga agtattgtgc taactggcta tctgatacaa ttaataaaac attttaaata
     1501 aaagtcaaat aaggttgcgt gcattagtta ttaaaaacaa aaaacagaga gttattttct
     1561 tttattccta ccggacacaa ataataaaaa atcatgcctt accaatagca aatccttcca
     1621 agctataaag cttgtcttta tggcttggaa ggattcaaaa ggttttctgt gctatcgctc
     1681 gaacctaccc acccaaaaaa ccgatcttgg gctgttatct gatatggtta aggcgactac
     1741 ttgacaaaga aaagacttac tctgcggagg atcaaaaaac cacccttgta gagggctgtt
     1801 atctgatatg gttaaggcgg ttacttgaca aagaaaaaaa accagttaaa acagaagaaa
     1861 agacagaacc cctaatgcta cggtcgccaa actcatcgca ttaggggttc agggaaaagg
     1921 aactgcttat gtttaacaac gataatcaac aaaattttgt aagtcaaatg cctgtgaagc
     1981 cttactgctc tgatgatttg agcactggac ttcgtatcag gaagaaagaa aaagcactgg
     2041 aaatgctcta catacaagca aaccagcctg ccatccaaac ctgtttgctt ttcgatctcg
     2101 atgatgacaa ctgtttctat aaattcgaag atgtcgggct tccagtaccc cacttcataa
     2161 cgaaaagccc gaactctgga agatgccact atggatacat acttcagaac ggagtgtgta
     2221 agacacaaca ggcacgatta aagcctctaa ggtatgccgc agccatagaa aacggtatgg
     2281 ctaacaaatt agggtcagat ccgggatacg ttggactaat cactaaaaac ccgctaaatc
     2341 cacactggtc accctactgg tctggggctg atctatatga cttagattat ctcgccgatt
     2401 ttgtagacct aaatgctcct aacccaaaag aaaagagagg gcaggagtat ggacttggcc
     2461 gaaatgttaa cctttttgac gatctacgct tgtatgcgta taggcacgtt ttagagttca
     2521 aagattcatc aagttttgat tcttggatgc gagaaataga gaaccaagcc attaatttaa
     2581 atacgttatg taatcctaag aacttactac cttttggaga agtaaaggca acagccagaa
     2641 gcataggaaa atggacttgg aaaaatttta gtcgttcagc attctccagc gttcaagctc
     2701 accgaggaag aatgaacggt ggcaagaaga aaaataacta taaaaaacaa tcaaatatag
     2761 aagaaatgat cagtttttta ggaggtgaga aatgagagtc agcagaaatg ggttaacaca
     2821 aaaacaactg gcagaaaaat ttaacatctc agtcacgaca gttataaaat acacagccat
     2881 agaaagggaa gagtatgaat caaaagccaa agaaagacgc ctgaaagctt acacaatgcg
     2941 ttctaacggc ttttccctta aagatatagc attagagctt ggctgttctt acaacgcagc
     3001 agcggccctt gtgaaacgat acaagcaatt agataataaa ggcactatat gaaagatgga
     3061 tttgctggaa tattagctgt ggctttctat gtcgcgggct ttgttggatg gttaacttct
     3121 gttatctctg atgcttctca tagtaggata ggatgggtta ttgctgatat tcttgtcttc
     3181 cctatgggag tggttagagg tgtaggaatg tggtttggat tcttctaacc catacaacaa
     3241 aaaaatagct ctcatgagct attttttttg tctatatctt tttcaatgta attaaaaaga
     3301 aagttaacca tagattttat atttttaata tcatcattta tatttgagtc tttctctttt
     3361 agcttctttc tgtttttggt ttttaataac ttgttgcgca agctttcttc tttcttcaat
     3421 tgtgcttctg acttcatttt ttcttgtatt aacaaaaatc ggtctgatga tttttgaaaa
     3481 aaattgaaca aatccactaa aatgtcttct tgtaaatcca attctaaatt caatcgtgtt
     3541 agcttctgct ctatttctaa actgtcgatc tctgactgtt tcagcaatct tattaattcg
     3601 ttgttgttca aaatcaattg cttcaatctg tcttgttctt tcttctcttt ggcttttttc
     3661 ttctcttggt tcagttttat catctctatt attttgctca tttaatacct cactaacatt
     3721 cttattatta tca
//
