LOCUS       NZ_CP056428            25549 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00515 plasmid pRHBSTW-00515_4,
            complete sequence.
ACCESSION   NZ_CP056428
VERSION     NZ_CP056428.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148644
            Assembly: GCF_013735895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 25549)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 25549)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00515
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 92.2829444239567x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,893
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,598
            CDSs (with protein)               :: 5,598
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 71 of 170
            Pseudo Genes (incomplete)         :: 106 of 170
            Pseudo Genes (internal stop)      :: 28 of 170
            Pseudo Genes (multiple problems)  :: 31 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056428.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..25549
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00515"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00515_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            13..714
                     /locus_tag="HV227_RS29020"
                     /old_locus_tag="HV227_29025"
     CDS             13..714
                     /locus_tag="HV227_RS29020"
                     /old_locus_tag="HV227_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172001.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_258923675.1"
                     /translation="MTDKSLSLKSPASPTGIAKQSPCYNVASRKRRRFLNRWIKSLATE
                     AGRIKIKNELRRRIRHNKYWVNEANKFGLEALCELMLAILDDLDLRDWQTIHNLETLAE
                     RAGLATRSDAGHKSISRASRGCDRLSWLNAIISEKAPFNPYDARCACKHIEVTEDFFAI
                     LGIPLKQVYRERARLLKADQNEIISSGDVRLIAIRVENWTRKAAAGLARMKARRDAARQ
                     RKQEYYSLTFA"
                     /name="RepA family replication protein"
     gene            1790..2119
                     /locus_tag="HV227_RS29025"
                     /old_locus_tag="HV227_29030"
     CDS             1790..2119
                     /locus_tag="HV227_RS29025"
                     /old_locus_tag="HV227_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032442682.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDTK
                     PLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEVLN
                     ETGKS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2100..2381
                     /locus_tag="HV227_RS29030"
                     /old_locus_tag="HV227_29035"
     CDS             2100..2381
                     /locus_tag="HV227_RS29030"
                     /old_locus_tag="HV227_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780222.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDNL
                     TKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2621..3646
                     /locus_tag="HV227_RS29035"
                     /old_locus_tag="HV227_29040"
     CDS             2621..3646
                     /locus_tag="HV227_RS29035"
                     /old_locus_tag="HV227_29040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849183.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_228725604.1"
                     /translation="MYEQTSGCTPLLMCGDSLEVLAQLPPDSIDFIMTSPPYWGKREYD
                     NGGIGLEIDHMDFVKNLAKICLQLKRVLKPTGSFWLNLGDTYKDKNLLGLPWRVAFELT
                     DNQGWILRNSVIWNKLKGGMDNSADRLGNVHENIFHFVKKNKGYYYNADAIRTKSRDAK
                     VVNGSVISATGVSGVRYKRQIELSTTLTPLEKVNALSALNGMLNDISTGKYSDFRMVIR
                     GQQRTTHSESEKVSGRAKELRDKGYYFLRYHPKGSKPADVWDILPEDTQKRKSHFAAYP
                     LDLCRIPILATCPEGGIVLDPFSGTGSTLIAAYELGRKSVGIDISDSYLKLTMERFNLL
                     K"
                     /name="site-specific DNA-methyltransferase"
     gene            3643..4488
                     /locus_tag="HV227_RS29040"
                     /old_locus_tag="HV227_29045"
     CDS             3643..4488
                     /locus_tag="HV227_RS29040"
                     /old_locus_tag="HV227_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013510462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="WP_032722772.1"
                     /translation="MSKIIEIFGLDTHSTQDWERVIQEQRCPFRQRTCFKTRKSESSVA
                     IGTCSVLYGRDPRPIMICPARLLERGQIFADCLHLLTNHEPGNEFHVVPEVKIPGGNVD
                     FFLASVRNGKVRDFVGIELQTMDTTGTVWPERQRFLREVNIIPSDDAEHSVRPYGINWK
                     MTAKTILVQMHHKVKTFEHINKKLVLVVQNHLLNYMSNEFNFSHLNQPARIGDSLHFHS
                     YSMERIGEEYRIEMNARLSTDDDGISQCLGLQAEARIELEQILLILERKIGPMTLFRMF
                     "
                     /name="NotI family restriction endonuclease"
     gene            <4502..4673
                     /locus_tag="HV227_RS29045"
                     /old_locus_tag="HV227_29050"
                     /pseudo=""
     CDS             <4502..4673
                     /locus_tag="HV227_RS29045"
                     /old_locus_tag="HV227_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4981..5475)
                     /locus_tag="HV227_RS29050"
                     /old_locus_tag="HV227_29055"
     CDS             complement(4981..5475)
                     /locus_tag="HV227_RS29050"
                     /old_locus_tag="HV227_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="WP_227502068.1"
                     /translation="MTENISISEIIFISDSRGFIKKFAEDKHLSTREVSSAEEAEYAII
                     FRDKRQDIGDFKLTKNAKVKVVLVSLTLVVNKDKGDKFDVYIGRGTIWGNPYQIGVDGD
                     RDEVIRKFKYDFDKGFLKPFDNIEKNALSLKGKIIACHCKPYACHGDVIADYVNSLDDG
                     E"
                     /name="DUF4326 domain-containing protein"
     gene            complement(5544..5996)
                     /locus_tag="HV227_RS29055"
                     /old_locus_tag="HV227_29060"
     CDS             complement(5544..5996)
                     /locus_tag="HV227_RS29055"
                     /old_locus_tag="HV227_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="WP_032722770.1"
                     /translation="MKNVKIHTIGFTKKTAEHFFNALKKNGVIRIIDTRLNNYSQLSGF
                     AKAKDLEFFLKKICDIDYVYRSELAPTKKILDDYKDKKITWEQYSIEYLSLMEQRLISS
                     KLSGIDVDNGCLLCSEDKPHHCHRRLLAEYLKQQWSNQKTNINHIL"
                     /name="DUF488 domain-containing protein"
     gene            complement(5997..6644)
                     /locus_tag="HV227_RS29060"
                     /old_locus_tag="HV227_29065"
     CDS             complement(5997..6644)
                     /locus_tag="HV227_RS29060"
                     /old_locus_tag="HV227_29065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045286760.1"
                     /translation="MRTVHQLLCLAKSWKNGGFCIAGKNDDNQWVRTITANPSNNAIFH
                     HGNAIQPLDFFQATTINHAPQGHQTENHIIDESVIFTVNGHCAADDLDDYVDTPATLWG
                     TGTSSNYGINDKIIIGQRVVNNSLYLIKPDDTVIVQMQNEYGTDKLRIRFSYNSDEYLL
                     TTTDMGIQNYFREEDNRLNDGEEEELDGVSYLTISLAEPLNGSLYKVVAGVF"
                     /name="hypothetical protein"
     gene            6905..7552
                     /locus_tag="HV227_RS29065"
                     /old_locus_tag="HV227_29070"
     CDS             6905..7552
                     /locus_tag="HV227_RS29065"
                     /old_locus_tag="HV227_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815856.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_004178130.1"
                     /translation="MTAVIALANGKGGVKKSSMSRAIATEYTKAGWGVLGLDMDIDQGS
                     FLDWGQRRLRNGFEPPVTIQGCGTVSQAKKQIESGQWDLIVIDSAAYATKSLLSLIDIV
                     DLLVLPTGFSVDDLRTTVTTVNALRNKGHSTDKMAVVFAGVSESESEYRAALEYLQPCG
                     VPVIPGAIPFLTSFSQAQDKGLALTESPFPGPRQKADDVVQGVIDRLTTLTQ"
                     /name="ParA family protein"
     gene            7593..7826
                     /locus_tag="HV227_RS29070"
                     /old_locus_tag="HV227_29075"
     CDS             7593..7826
                     /locus_tag="HV227_RS29070"
                     /old_locus_tag="HV227_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004178129.1"
                     /translation="MANVPAKPRNTKGEPPSQADTEHGFNPTRKSADGRKAQLNLYIDM
                     ELKREFRSIAAANDMKHGELFEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(7864..8205)
                     /locus_tag="HV227_RS29075"
                     /old_locus_tag="HV227_29080"
     CDS             complement(7864..8205)
                     /locus_tag="HV227_RS29075"
                     /old_locus_tag="HV227_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032722766.1"
                     /translation="MIFAKFQSLTHKIDTMVIRDIKREMPLKYWSFKVAEWIARIGMIG
                     FVCTFLTYFGLGLLMQHSGQNLPESFTDGCAQAIVALIAIALVGFLVRGGLYVDLEKRI
                     LDKWQGYVQ"
                     /name="hypothetical protein"
     gene            complement(8266..8739)
                     /locus_tag="HV227_RS29080"
                     /old_locus_tag="HV227_29085"
     CDS             complement(8266..8739)
                     /locus_tag="HV227_RS29080"
                     /old_locus_tag="HV227_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_032722765.1"
                     /translation="MKCRSRLLVFLPLLFPALCLADFSGRVVRVVDGDTVQVLADGKMM
                     KVRLNGIDAPESGQPFGQRSKQNLLELAVQKQTVVIANNTDRYGRWLGTLMIDGIDINA
                     EQVKAGMAWAYRYHGRASDESMLRLEEEARRHKIGLWSSSDPVEPWKWRKQNK"
                     /name="thermonuclease family protein"
     gene            complement(8776..9531)
                     /gene="mobC"
                     /locus_tag="HV227_RS29085"
                     /old_locus_tag="HV227_29090"
     CDS             complement(8776..9531)
                     /gene="mobC"
                     /locus_tag="HV227_RS29085"
                     /old_locus_tag="HV227_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_023316386.1"
                     /translation="MLIHNVAERKEAANRKMLALLTFLKEETYSDFKTLKKVVGFKGKS
                     HRPTYALLNKAVALGFLIKHEYPVIAGKKSLWGITMQGLAMVVKPDDKVFPGYFEPGKL
                     KYWTLEHRLLNHRVRIALEEKGGQGWLNGDRGEFIARYPGVRHRPDGIITLDSGAIVAV
                     ETERSMKTRARYINIINSHLAASDAGRWHYAMYVMPDDKTKTSLIRLFDSIKTVMRNNV
                     PVPFDAKNREMFLFRTIDELEQAAASGGQ"
                     /name="mobC"
     gene            complement(9540..11276)
                     /locus_tag="HV227_RS29090"
                     /old_locus_tag="HV227_29095"
     CDS             complement(9540..11276)
                     /locus_tag="HV227_RS29090"
                     /old_locus_tag="HV227_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017346477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_181486821.1"
                     /translation="MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLP
                     REERKNAPFWLKSVACVSIFFFIFGTISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIF
                     TAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNTRTDVRKVKELLPDPIEYNPLDYI
                     DLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGITATVLLYQAILLGEAVFV
                     EDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLAK
                     KGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVN
                     SINATKGFSLKEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTV
                     AIFLDELKYHLSRPALEGLGTARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPEKVGFVFTSTELPRATKMFQVQVKKEDIELLSFEYKQATPEKNESQEHKPSI
                     NL"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(12054..12359)
                     /locus_tag="HV227_RS29095"
                     /old_locus_tag="HV227_29100"
     CDS             complement(12054..12359)
                     /locus_tag="HV227_RS29095"
                     /old_locus_tag="HV227_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045286759.1"
                     /translation="MINENLFIKNIHSKNQDRISVALVYDTLSKEAHRGCGLYYEIYES
                     CFIGLLRDHLSELNEADANKLRRYAESKGTKIDDASYSEALEAERECRSEIYREQM"
                     /name="hypothetical protein"
     gene            complement(12393..12698)
                     /locus_tag="HV227_RS29100"
                     /old_locus_tag="HV227_29105"
     CDS             complement(12393..12698)
                     /locus_tag="HV227_RS29100"
                     /old_locus_tag="HV227_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_032722794.1"
                     /translation="MKKTVITAAVLCLTAGTPIYSFAADPCEVVLCMYGKATGNSGGSE
                     CSSAERAFFKINAFKKHHRFNPGKTFDMRKEFLGECKAADPESISKILSKFGRVKG"
                     /name="conjugal transfer protein"
     gene            complement(12725..13132)
                     /locus_tag="HV227_RS29105"
                     /old_locus_tag="HV227_29110"
     CDS             complement(12725..13132)
                     /locus_tag="HV227_RS29105"
                     /old_locus_tag="HV227_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_071993161.1"
                     /translation="MIKKLKVIMLAAIVTGCSSPPPPVPVEWDKAAEPLNTSLPQWSDN
                     NVIVPSPTVNGKWTSSISAQSFYDTIWTPAVFYAVAHSTRIVVTAQSGTDFFNAKSWLR
                     QNGAKGVIEYQPVFNCLTCRETTIYFSRQSI"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(13129..14154)
                     /gene="virB11"
                     /locus_tag="HV227_RS29110"
                     /old_locus_tag="HV227_29115"
     CDS             complement(13129..14154)
                     /gene="virB11"
                     /locus_tag="HV227_RS29110"
                     /old_locus_tag="HV227_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227785.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000125558.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELYTKINGVWE
                     QHAVPLSYEDCYNFARCLAKHHGDNIEDIKPGLSATLESGERCQVIVPPACERDTVSIT
                     IRKPSKVQIPHQSYIDAGFYNRVTGEEKTETHDEELIALYNTKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIGYIPVHLRISTIEDNPEITFFIHKNYVHLFYPSESSDEKGS
                     IVTPASLLRWNYRMNPDRILLTEVRGAEAWDFLKITGSGHEGSMTSIHAGSAKEAIDGF
                     ITRCYENPQCAQLPYTFMLRKVLDSLDVIVSIDLDGNVRRMNDIYFRPIHRNQYFEEMK
                     A"
                     /name="virB11"
     gene            complement(14144..14464)
                     /locus_tag="HV227_RS29115"
                     /old_locus_tag="HV227_29120"
     CDS             complement(14144..14464)
                     /locus_tag="HV227_RS29115"
                     /old_locus_tag="HV227_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032722553.1"
                     /translation="MTRENKKRLAILVMYIARIVGQSLRDVPLVLLIAGFLFLLFAPPT
                     AWMAINQIWYHLRPLTDREAVMLGKSVSGLLQLAWLVAASSRFILSVDELSSRMKEVKD
                     DR"
                     /name="hypothetical protein"
     gene            complement(14476..15738)
                     /gene="virB10"
                     /locus_tag="HV227_RS29120"
                     /old_locus_tag="HV227_29125"
     CDS             complement(14476..15738)
                     /gene="virB10"
                     /locus_tag="HV227_RS29120"
                     /old_locus_tag="HV227_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_032722790.1"
                     /translation="MNDENKVPVPDEAQASPAHAEDDIATLEREARAKREAELLNAQDD
                     EEKDPVQPAVNKLKKRKRGKATAFLAIVAVALIFLAWGGNWVYRNILWQPTEEKKQDTT
                     PQTNKTDYRQRNDLGMSTDTAEEEPEQQDNGQNSAAGTGQAAPAAPPELNKASFLIRRD
                     GSATTQNQVKTRQQEMTLTSATTTGQQDSSAATPPGQPQNAAAPSPGQSPAPVRRIPYN
                     PDLYIPENTSIPCSLDRRFVSDRAGKLRCTVTTDIWSASGNTKLIEKGTTASLLYRAIA
                     EEGMKHGQGRAFIIATKLRTRQPPYLDIPLVDTSAAGELGEAGVDGWIDSHFWERFGGA
                     LMVGMIPDIGAWASNSAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEI
                     INLITGEDIDFSNIYTLKIKR"
                     /name="virB10"
     gene            complement(15785..16693)
                     /gene="virB9"
                     /locus_tag="HV227_RS29125"
                     /old_locus_tag="HV227_29130"
     CDS             complement(15785..16693)
                     /gene="virB9"
                     /locus_tag="HV227_RS29125"
                     /old_locus_tag="HV227_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_087749168.1"
                     /translation="MMKKLTFTALALMMCSAAWGAAIPQASRYDSRVQQVIYNPQNVTV
                     VNTKPGFMTTLVFDNDEAVISAKPGFDEAWEATPDANRVNVRPVALTQGAPGEDGNTTQ
                     VVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSTQQPAFQVSYRYPGEERDKASREAT
                     ARQREWEQKQQQASVQKALNSAQTPRNWSYTQYPGKGSFNIVPDFAYDDGRFTFVGFSP
                     SKSIPSVTKELNGKEHVVNSSIQKKGDFTVLVIQEVTPRLVLRSGNAVVGLENSGFGKV
                     HAADGSTVSRQVERVEKPEPN"
                     /name="virB9"
     gene            complement(16690..17373)
                     /locus_tag="HV227_RS29130"
                     /old_locus_tag="HV227_29135"
     CDS             complement(16690..17373)
                     /locus_tag="HV227_RS29130"
                     /old_locus_tag="HV227_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_004178111.1"
                     /translation="MSEQKLIAESRTFEQQMIERDKRATKAGFVVGGVGLLIAVLALVA
                     VVVMLPLKQTDVELYTVDNHTGRVEHVTRTSKTSLTATEAYQKAMAANYVKVRERYVYP
                     SLQDDYETVQVYNAPQVNDDYLALYAGKNAPDKVYKNGAHTVKVEILSNQITDATAPDR
                     VATIRYKKIIRRLADNSTRNEYWDARFTFHSDPDKEMSDAEREINYFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            complement(17366..17503)
                     /locus_tag="HV227_RS29135"
                     /old_locus_tag="HV227_29140"
     CDS             complement(17366..17503)
                     /locus_tag="HV227_RS29135"
                     /old_locus_tag="HV227_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039109361.1"
                     /translation="MKRLIALALLCALSGCAQNTLPSAFSGKGEPVNTPQIMAELNSHE
                     "
                     /name="hypothetical protein"
     gene            complement(17595..18671)
                     /locus_tag="HV227_RS29140"
                     /old_locus_tag="HV227_29145"
     CDS             complement(17595..18671)
                     /locus_tag="HV227_RS29140"
                     /old_locus_tag="HV227_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_004178110.1"
                     /translation="MAELGTFEFIDKQLIGGITQSTSGLMMEHSSMVMGLAAASATIYI
                     MWRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANVSGYLDGVIDAINGLKEGFSGGDN
                     IWQMLDTLWDKAKVLGKTLHDMDNSTYVKDEGMTAQFYVWLGIFILMVLTAFVSMVAEI
                     MILLLSITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTVLFSALSLRIVINFMTKILAQ
                     SQAGAAQSNIVELGAQVCLAGICAAILVYISAKVAAALAGASATVSMQGLAAVGIGAAA
                     FGAGKLAGGAVKETKRSAQDNIQGWKDANAGKPGKPGGELGYNASKARKYAIEQMIARN
                     EARRLAQSRESAVKKFNS"
                     /name="type IV secretion system protein"
     gene            complement(18683..18910)
                     /locus_tag="HV227_RS29145"
                     /old_locus_tag="HV227_29150"
     CDS             complement(18683..18910)
                     /locus_tag="HV227_RS29145"
                     /old_locus_tag="HV227_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_032722788.1"
                     /translation="MNKFIAIVALSLVAIISGCKEEAKTTKWYRDHPDELKVVYEKCQK
                     SGDASENCKNANEAHYQIQQLNAPTLKFHD"
                     /name="EexN family lipoprotein"
     gene            complement(18920..19630)
                     /locus_tag="HV227_RS29150"
                     /old_locus_tag="HV227_29155"
     CDS             complement(18920..19630)
                     /locus_tag="HV227_RS29150"
                     /old_locus_tag="HV227_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_012457111.1"
                     /translation="MKTHFYALIFACSLASPLAQAGGILVFDATAKLENVRQWQKEAKQ
                     WMETAQHYKDQIRAYKDQLATATGVRDIASFVDQAKSLKADLEKLRKPGQALNDLLLSG
                     GSSGQFDALYEKYKIFDTCNAERSGNYANVCKQQVINKAIQLEQTDEIQNQVSQTLGEI
                     NSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQWEMSVKAAEKREKVLEAERVKQW
                     NQQQLNAPTLKFHE"
                     /name="type IV secretion system protein"
     gene            complement(19649..22387)
                     /locus_tag="HV227_RS29155"
                     /old_locus_tag="HV227_29160"
     CDS             complement(19649..22387)
                     /locus_tag="HV227_RS29155"
                     /old_locus_tag="HV227_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_181486820.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLP
                     VQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMK
                     LNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLHGLI
                     RSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRP
                     FVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFSS
                     QLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFS
                     EETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDV
                     ALDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYM
                     AQLPGNYNLRPRKGELSSQNFVELAALHNFYPGKRDKAPWGDAMALLRTPSGDGYYINL
                     HNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGREDSFSPDAKTKRLTT
                     VYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILCTRNGS
                     TISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQD
                     GEFGWVFDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIF
                     MDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQIL
                     AANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKRGDTKRFAARVTLDLS
                     GIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(22400..22705)
                     /locus_tag="HV227_RS29160"
                     /old_locus_tag="HV227_29165"
     CDS             complement(22400..22705)
                     /locus_tag="HV227_RS29160"
                     /old_locus_tag="HV227_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_012457109.1"
                     /translation="MKLFSKVKELISRASAYVLAPAMSVFAASPAFADGFSRANTIMEK
                     VSSGLHGLAAITITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(22702..23412)
                     /locus_tag="HV227_RS29165"
                     /old_locus_tag="HV227_29170"
     CDS             complement(22702..23412)
                     /locus_tag="HV227_RS29165"
                     /old_locus_tag="HV227_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_032722783.1"
                     /translation="MLSTTAFLAVAMQCAATVHPSTSLDVARVESGYNPYAIAEIVPKP
                     ERKPGDKGFITHMPKSKEDAVSITNQIEAKGRRYSVGLMQITSTNFKRYGVTATDLFNP
                     CINLSVYEKVITDCYQRGGTLKRALSCYYSGNFSTGQQQEPAFSKTSYVQRIGYSPADT
                     RYAVPGTRDDIATPAATLKATPVDAPTRPRVVWPGAIVRGVPAQLRQKKAATVYYPAQV
                     VRGNRDVTTKEDEK"
                     /name="lytic transglycosylase domain-containing protein"
     gene            23411..23533
                     /locus_tag="HV227_RS29715"
     CDS             23411..23533
                     /locus_tag="HV227_RS29715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_258923676.1"
                     /translation="MATSEQKNGDQPDDEQNEQPASERKPGIGIVSIHCAVSLL"
                     /name="hypothetical protein"
     gene            complement(23530..23748)
                     /locus_tag="HV227_RS29170"
                     /old_locus_tag="HV227_29175"
     CDS             complement(23530..23748)
                     /locus_tag="HV227_RS29170"
                     /old_locus_tag="HV227_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032722781.1"
                     /translation="MRKPTYNVYVTQETQPGENGEKNTYWTKVGAAFAHNGKPGLNIMI
                     TPGISVSGKLVLLEPKDDAAPQAAPVS"
                     /name="hypothetical protein"
     gene            complement(23753..23968)
                     /locus_tag="HV227_RS29175"
                     /old_locus_tag="HV227_29180"
     CDS             complement(23753..23968)
                     /locus_tag="HV227_RS29175"
                     /old_locus_tag="HV227_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004178170.1"
                     /translation="MPKSVPGKSSTAVIYIGQKRYQELAKQAREISYLSESNIRPSTFL
                     QFLMDEFGEQARTELLRQLLAEKQKE"
                     /name="hypothetical protein"
     gene            complement(24040..24495)
                     /locus_tag="HV227_RS29180"
                     /old_locus_tag="HV227_29185"
     CDS             complement(24040..24495)
                     /locus_tag="HV227_RS29180"
                     /old_locus_tag="HV227_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172004.1"
                     /GO_process="GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0140673 -
                     transcription elongation-coupled chromatin remodeling
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_058645037.1"
                     /translation="MYEWYVLYCNNQDVERITRRVEGLDVEVFCPRYIKVTPRKDCRAV
                     RQEEKVLFPNYLFLCFDVNITHTSAITSIPGAHGFVSFGSTPCTVPKSVITAIECARLM
                     ALNPDEDAIECRNIAPDLLAKIQELSLIRSPLQRQVAFSKLLQNQYH"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            24746..25102
                     /locus_tag="HV227_RS29185"
                     /old_locus_tag="HV227_29190"
     CDS             24746..25102
                     /locus_tag="HV227_RS29185"
                     /old_locus_tag="HV227_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172003.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_011172003.1"
                     /translation="MVPKRLKEAREAAGLSQEKLSELVGVEGVNPRSRMSNYEAGRFAP
                     PFEFVCRVADVLGYPEYYFYIVNDVTAKLLLRMHRGEQNLHEDIEFEAKKMAEQLEDAR
                     KLVAQLTECLKHRP"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          2..714
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.779"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(15789..16690)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.671"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(16694..17373)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.73e-130"
     Typing          complement(17599..18671)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.721"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19653..22387)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="90.919"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgggcgg gtgtgactga taagagtctc tctctcaaat cacctgcttc acctacgggc
       61 atcgcaaagc aaagcccctg ttataacgtt gcctcgcgca agcgtcgccg gttcctcaat
      121 cggtggatta agtcgctggc gaccgaagcc gggcgcatca aaatcaaaaa cgaacttcgg
      181 cgccgcatcc ggcacaacaa gtattgggtc aatgaagcga acaagttcgg acttgaggct
      241 ctttgcgagc tgatgttggc catccttgac gatctggatt taagggactg gcaaaccata
      301 cataaccttg aaacgctggc cgaacgcgcc gggctggcga cgcgcagtga tgcgggtcac
      361 aagtcgattt ctcgtgccag ccggggatgt gatcgcctct cctggcttaa tgccattatc
      421 tcggaaaaag cccctttcaa cccttatgac gcccgttgcg cctgcaagca cattgaggtt
      481 acagaggatt tctttgccat tctcggcatc ccgctaaagc aggtttaccg ggaacgcgcc
      541 aggctactta aggccgacca gaatgaaatc atatcatccg gggatgtgcg gttaatcgca
      601 atcagggttg aaaactggac gcgcaaggcc gcagccggat tagccaggat gaaagcgagg
      661 cgtgacgcag ctcgccagcg caaacaggaa tactactccc taacctttgc ctgatccccg
      721 cctacttccg ccttagctgg cggggattct gcacgtctgc aataaacctc agtacaaaaa
      781 acagccttaa cgtacaataa tttccgaaat ccaaacaatc cctcctatct gaatgacgga
      841 tttttcggcg gttccggcgt agccccccac aactgctgta tagcttgtgg gtaagtgata
      901 atgaagtgca aacgatatga tgcaattact cttttggttt atcttttaat tacttacgta
      961 ttaaaagata aaccaaaaaa taaaagacat tactgttatc ccctttaaaa agtggataac
     1021 cgaacagcat cggcgcaagc gcctcaaaag tagctcccca cgctaaagcg cggggcattc
     1081 agatcggaat cgtgagaggg ctaacagctc gcctgttagc cgtcgaggga ttggacaaat
     1141 ccggccacgc cggatgcgtc agcaaatatc gctgaacgtt ttgctgacac gctcgtaagc
     1201 ggctctgacg acccgctaac gcggagatac gccccaactg cggcttcgcc ttgttgtgac
     1261 cactccgacc gcgcacagaa gcccctgagc cgcttttagt gggttatggc tttgcagggt
     1321 aagggatgga taggcgatcc ctttcgatcc ctcactggta tcggcagcta accctcagtg
     1381 ctacctgtca tttcatcctg ccgccttaca tgcgctgacg cgcatcaatc cccgttcctc
     1441 aatggaaggg gtctgaagtc caatggaacg aaaacgtacg ttagtgaagt aactgtctga
     1501 tatatcaaaa cataatggac atagaaaaat gcctttgaaa tggtgtcttt ttaatcgaaa
     1561 aacgtcattt aacggacagt gttgtctact aatcgacttt tcagacgttt atttttcgct
     1621 taccgcacgt tttcgtacgg ttggccctca aacccctgga acgaaaactc acgctaacag
     1681 ggtagtgacc gggtagccgc gataactgcc aacaatatta gcttacaggt taattttacc
     1741 ttgcctgaaa ttagcctata ggttaatatt aatggaaatg ggaggtcata tgtttagcgt
     1801 cgtttatcac ccggaagcca gggaagaagc aacagcatta cccgtaaaaa tcagggtgaa
     1861 gtttgaccgt ctcatcggca aactggagta tgacgcccga ttactgcggg agccggacac
     1921 gaagcccctg ggcgacggac tttttgagat ccgcacgatg gggacggaca tagctagggg
     1981 aatatgggta taccacaaag gcaataccat catcatgctc cgggttttca tcaagaagtc
     2041 acagaaaacg ccagcgaaag aaatcgatct cgccaaaaag cggttagcgg aggtacttaa
     2101 tgaaactggt aagtcataaa gaactgcata acgagtggat gcaggacgac gaatatcgcg
     2161 ctgcatggca ggaagaagaa cgcaaggaga agctcaggga gctacttgcg gaatggcgca
     2221 agcatgacaa cctgacaaaa gcacaggttg ctgaacgcat gggggttaca cctccggtta
     2281 tttctcgctt ggaaaacaac attaccaaag ccagcatcga cactctgacc cgctatgctc
     2341 acgcctgcgg catcaaaaaa cctgtcattg ccctgtactg aacaaccagc cctccggggc
     2401 tggccttttt gtttcggttc ggcttcagac cctctgagag gcagaccaat ctgaggtgaa
     2461 taatggtatt ctttatatat aaatctattt ttttttgaat aagttacggt atagctagat
     2521 gaaacagaac ttacaaagtg aactagaaag ccatataaaa ttagatggtt atgagaaaaa
     2581 aataaattat tttaatgatt cattctctga gtctttagcg atgtacgagc aaacatcggg
     2641 atgtactcca cttttgatgt gtggtgattc tcttgaagtt ctggcacagc tccctcctga
     2701 ctctatcgat ttcatcatga cttccccccc gtactggggg aaacgagaat atgataatgg
     2761 aggaattgga ttagaaatag atcacatgga ttttgtcaaa aatttggcga aaatttgcct
     2821 acaactcaag agagttttaa agccaacagg ttcgttttgg ttaaatcttg gggatacata
     2881 taaagataaa aatttattgg gattaccttg gcgtgtggct tttgaattaa ccgataatca
     2941 aggatggatt cttaggaata gtgttatatg gaataagttg aaaggaggca tggataacag
     3001 cgctgatcgc ctcggaaatg ttcatgagaa catttttcac tttgttaaga aaaataaagg
     3061 ttattactat aatgcagacg caattcgtac taaatctaga gatgcaaaag tagtaaacgg
     3121 ttccgtaatt tctgctactg gtgtttcagg tgttagatac aaaagacaga ttgagctttc
     3181 tactactctg acaccgcttg aaaaagtcaa cgctctttca gcattaaatg gcatgcttaa
     3241 tgatatttca accggaaaat attctgattt cagaatggtg attcgtgggc agcaaagaac
     3301 cacacactca gaaagtgaaa aagtttctgg aagagcaaaa gaacttagag ataaaggtta
     3361 ttattttctg aggtatcacc ctaaaggtag taaacccgct gatgtttggg atattttgcc
     3421 agaggataca caaaagagaa aatcccactt tgctgcttac cctttagacc tttgtagaat
     3481 cccaattctg gcaacttgtc ccgaaggtgg cattgtattg gacccatttt ctggtactgg
     3541 ttcaacattg attgctgcat atgagttagg tcgtaaatct gttgggattg atatatcaga
     3601 tagctattta aagctcacta tggaaaggtt taatctatta aaatgagcaa aatcattgaa
     3661 atttttggat tggatactca ctcaactcaa gattgggaac gcgtgattca agagcaacgt
     3721 tgccctttta ggcaacggac atgcttcaaa acccggaagt cagagtcgag tgtcgccatt
     3781 ggtacatgtt ctgtgcttta cggaagagat ccccgtccaa taatgatctg tccagcaagg
     3841 cttctcgaac gagggcagat atttgcagat tgtctgcact tgcttactaa tcatgaacca
     3901 ggtaatgaat ttcatgttgt tccagaagtt aaaatcccag gaggtaatgt tgacttcttc
     3961 ttagcctctg taagaaatgg aaaggtaaga gattttgtgg gtatagaact gcaaacaatg
     4021 gatacaacag gtactgtatg gccagagaga caacgttttc ttagggaggt aaatatcatt
     4081 cccagtgacg acgcagagca ttcagtcaga ccatatggta ttaactggaa gatgactgcc
     4141 aaaactattt tggttcaaat gcatcataaa gtgaaaacat ttgaacatat aaataaaaaa
     4201 cttgtattgg tggtgcagaa ccatttgttg aactatatgt caaatgaatt taatttttca
     4261 catttgaatc agccagcaag gattggagac tcattacatt ttcattcgta tagcatggag
     4321 cgaataggag aagaatacag aattgagatg aatgcaagac ttagtactga tgatgatggt
     4381 atttcacaat gcttaggttt gcaagcggaa gctcgtattg agcttgaaca aatattacta
     4441 atacttgaaa gaaaaattgg tccaatgaca ttatttagaa tgttttgata atatctcacg
     4501 tgcggaatgg cgtaagcatg aaaacctgac aaaagcacag gttgctgaat gcatgggggt
     4561 tacgcctccc attatttccc gcttaaaaca acatcaccaa agccagcatc gatactctga
     4621 cccgccacgc tcacgcctgc ggtatcaaga aacttgtcgt tgccctgtac tgaacaacca
     4681 gccctccggg gctggccttt ttgttttgac cagtcccaaa atttggttcg ccaaatgcct
     4741 tttatgttaa atgggagttt ttttactcaa aaatagggct cttgagtcat taaatagtcg
     4801 aaaaggcttt ttctatagct tttaggtgca ttttagctat acaatcttaa tgtaatattt
     4861 ttgttaaaat tgagtattct tgtaacaaat ttaacacttg tcttcttcag ctcttttttt
     4921 ccattaacgc tacaaaattc atcaggaaag ttcttgccag aatcagacct gtatcaaatc
     4981 tcattcaccg tcatcaagag agtttacata atcagctatg acatccccat gacatgcata
     5041 cggcttgcaa tgacaagcga taatctttcc cttaagcgat agagcatttt tttcaatatt
     5101 atcaaagggt tttaaaaatc ctttgtcaaa atcatattta aattttctta ttacttcatc
     5161 tctatcgccg tctacaccta tttgatacgg attcccccat atagtgcctc tacctatata
     5221 tacatcaaat ttatcgccct tatctttatt gacaactaat gtcagactta caagcacaac
     5281 ttttacttta gcatttttag ttaatttaaa atcgcctata tcttgacgtt tatctctaaa
     5341 aataatcgca tattcagcct cttcagcaga actcacctca cgagtgctga ggtgcttatc
     5401 ttctgcaaat ttttttataa accctcttga atcagagatg aagattattt ctgatatgct
     5461 tatattttca gtcaaaaagt ttaatttcct ttggaatttc ccaaagcaat tgaactcttt
     5521 gctatattct aaaaaaagta cactcataat atatggttaa tgttagtttt ttggtttgac
     5581 cattgttgtt ttagatactc tgccaaaagc cttctgtggc aatgatgggg cttatcttcg
     5641 ctgcaaagaa ggcatccatt atctacatcg ataccagaca gctttgatga tataagtctt
     5701 tgttccataa ggcttaagta ttctattgaa tactgctccc aggttatttt tttatcttta
     5761 taatcgtcta ggattttctt tgttggagct aattctgatc tgtatacata atctatatca
     5821 catatctttt ttaagaaaaa ttcaagatct tttgcttttg cgaaaccgga taactgtgag
     5881 tagttattca atcttgtgtc tattatgcga atgacgccat ttttctttaa tgcattaaag
     5941 aaatgctcag ctgttttttt tgtaaaacca attgtatgta ttttgacatt tttcatttaa
     6001 aatactccgg ctactacttt atataaactt ccattaagtg gctcggctag actgatagtt
     6061 agataggaga caccatcaag ttcttcttcc tcgccatcat ttaacctatt atcttcctct
     6121 ctgaaataat tttgtattcc catatctgta gttgtcagta agtattcatc agaattatag
     6181 ctaaatctta tccttagttt atcagtacca tactcgttct gcatctgtac tatcaccgta
     6241 tcatcaggtt tgatcaaata gagtgaattg ttaactactc tctgcccaat gatgattttg
     6301 tcatttatac cgtagttact gcttgttcct gtcccccata gagtcgcagg cgtatccaca
     6361 taatcatcta aatcatcagc ggcacaatgc ccattcacag taaagatcac gctttcatca
     6421 atgatgtggt tctctgtttg gtgtccttga ggggcgtggt taatggttgt tgcttggaaa
     6481 aaatcgaggg gttggatggc atttccatga tggaatatgg cgttattact agggtttgct
     6541 gttattgttc ttacccattg attatcatcg ttttttcctg caatacaaaa tcctccattt
     6601 ttccaagatt tagcaagaca aagtagttga tgaactgttc tcattttctt cccttacatt
     6661 tttccattag aagccgctag gcgaagcatt aattattgct acatatatca gatccatcct
     6721 atttttcaat ctatgtgtaa caaaatctta catgcatttt gattggtttg ttatcaatgg
     6781 cctccatgca tacattttgg ggtattatta tatttttata taaaaatatt tactgatgat
     6841 ggcgcctgga ttagacttat agcgatacct atttttatag gaaaatattt atataggggg
     6901 gggtatgacc gctgttattg cactggctaa cgggaaaggt ggggtgaaaa agtcctcgat
     6961 gtcacgggct atcgccaccg agtatacaaa ggcaggatgg ggggttcttg ggctggatat
     7021 ggatatcgat caggggtctt tcctggactg ggggcaacgt aggttgcgga atggttttga
     7081 acctccggtg acaattcagg gatgcgggac ggtttcacag gcgaagaaac agattgaatc
     7141 cgggcaatgg gatttgattg tcatcgacag tgctgcgtat gcgacgaaat cgttactcag
     7201 cctgattgat attgtcgatt tgctggtact gccgacaggg tttagcgtgg acgatctacg
     7261 aacgaccgtt acgaccgtaa acgcgctgcg taacaaaggg cattcgacag ataaaatggc
     7321 ggtggtgttt gcgggggtaa gtgaaagtga gagtgaatat cgcgcggcgc tggaatacct
     7381 gcaaccctgc ggcgttcctg tcattccggg cgctattcct tttctgacat cgttcagcca
     7441 ggcgcaggat aaagggcttg ctcttacgga gtcgcctttt cctggcccac ggcaaaaagc
     7501 tgatgatgtt gtgcagggtg tgatcgaccg actgacaacg cttacgcagt agtatataat
     7561 aatataatta tatttatcta tgaggtgata ttatggcgaa cgtcccagca aaaccgcgca
     7621 ataccaaagg tgagccgcca agccaggcag acacggaaca cggctttaat cctacccgga
     7681 aaagtgctga tggccgtaag gcacagctta acttatacat tgatatggag ctgaaacgtg
     7741 agttccgctc cattgctgcg gccaacgata tgaaacatgg ggagctgttc gaggcgtacc
     7801 aggaattctg gaaaaaacat cattaagtca cccggtatcc cggccactgg ccgggtgata
     7861 ccatcactga acgtagccct gccacttatc caggattctt ttttccagat cgacgtacaa
     7921 cccgcctctg accaggaaac cgaccagggc aatagctatc aatgcaacga tggcttgagc
     7981 gcatccatca gtaaacgatt ccggcaggtt ttggcctgag tgctgcatga ggaggccgag
     8041 tccgaaatac gtaagaaagg tacaaacaaa gccaatcatt ccgattctgg cgatccattc
     8101 cgccactttg aatgaccagt atttcaacgg catctcccgt ttaatgtcac gaataaccat
     8161 tgtgtctatc ttatgggtca gtgactgaaa tttagcgaat atcataagtc cctcgtccgt
     8221 ctaaaaatta accttaccat atcagaatta tataaaaata taatcctatt tattctgttt
     8281 gcgccacttc cacggctcca ctgggtcaga tgacgaccac agcccgattt tatgccgtct
     8341 ggcttcttct tcaaggcgta gcattgattc atcactggca cggccatgat atcgataagc
     8401 ccacgccata cccgctttaa cctgctctgc gttgatatcg ataccgtcaa tcatcaatgt
     8461 tcccagccag cggccataac gatctgtatt gttggctatg accaccgtct gtttctggac
     8521 ggccagctct aacaggtttt gcttcgagcg ctggccgaac ggttgccccg attccggggc
     8581 atcgataccg ttaaggcgaa ctttcatcat tttgccatcg gcgagcacct ggacagtatc
     8641 accatcgacg acccgaacaa cacggccaga aaagtccgct aggcaaaggg cggggaaaag
     8701 caggggcaga aacacaagca aacgagatcg gcatttcatt tgtcacacct tataaaaata
     8761 ggtttatata aataatcact ggccgccgct ggcggctgct tgctccagtt catcaatggt
     8821 gcggaaaaga aacatttcgc ggtttttcgc atcgaacggc accggaacgt tgttcctcat
     8881 taccgttttg atggagtcaa acagccggat taacgatgtt ttggttttgt catccggcat
     8941 gacatacatc gcgtagtgcc agcgtccggc atcgctggcc gccagatggc tgttaatgat
     9001 gttgatatag cgtgcgcggg ttttcattga gcgctcagtt tcgacggcga cgattgcgcc
     9061 gctatctagc gtgataatcc cgtcaggacg atggcgaacg ccaggataac gcgcaataaa
     9121 ctccccccga tcaccattaa gccagccttg cccgcctttc tcttccagtg caattctgac
     9181 ccgatgattt aggagccggt gttccagtgt ccagtatttg agtttccccg gctcaaaata
     9241 accggggaat actttatcat cgggtttaac caccattgcc agcccttgca tggtgatccc
     9301 ccatagtgat ttttttccgg ctattaccgg atattcatgt tttatcagaa accccaatgc
     9361 aaccgcttta ttcagtagcg catacgttgg gcggtgactt ttacccttga acccaacaac
     9421 ttttttcagc gttttaaaat cggagtatgt ttcttcttta aggaatgtca gcaatgcaag
     9481 catttttctg tttgcggctt ctttcctttc tgcaacgtta tgaataagca tttcagccct
     9541 cacagattga ttgatggctt atgttcctga ctttcatttt tttcaggtgt ggcttgttta
     9601 tactcaaatg aaagaagttc gatgtcttct tttttgacct gaacctgaaa catctttgta
     9661 gctcttggta gttcggttga cgtaaataca aaaccaactt tttcagggag atttaaaaac
     9721 atattactgt caacatagta actttctgcc tgtcttatca ttcggtctgt atccactttc
     9781 tccgttaatg acaaatcagt ccgtactttc ctcatttcat catcaaccag gatagagcct
     9841 gtcatttttg ccgcccattc tgctgtttcg gggttttgga ttttataaat taatttgaat
     9901 ttggcgtttt caatgatagc gccaataact gaatcaccat ttaaatcaga cgggcagtca
     9961 cgcagatcgt ctatcgcctg aaaagccatg aatatatgca cgcctttatc tcgcgccgtt
    10021 ccaagtcctt caagagcagg gcgagaaaga tgatatttca actcgtcgag gaaaatagca
    10081 acagtacgcg gggtgctgtt tattctgtcc ctggtctctg ctatttgtat cagccgggtc
    10141 agaatcattc tttgtgcaga aattatcttt tgattgcgcg ttgaaccaat aatgtagcaa
    10201 caaccacctt catcaaaaac ctcttttaat gaaaatcctt tggtggcatt aattgagtta
    10261 actaaagcaa tttcttccag ctcgccacaa aatgcgggaa catcttcacg taatgactga
    10321 acaaagtcag tgttaaataa atcacgaagt gtcattcctt tttcataaat tgcagatgta
    10381 tttctggctg cgcgacggtc agagatgcga taaaaatctg aagcctcccc ttttttagcc
    10441 aggctaaagc cagcgttaaa taattcctcc agttgttcat gagagatatc agccagtaaa
    10501 tcaagctgga aattgagctt gtttaaattt ataagtgtga atttcttgcc tgctttttta
    10561 catgcttccc taagtacgtg aggtgcccat tcatcatctt ttggatcttc tacaaaaaca
    10621 gcctcaccta aaagaatagc ctgataaagt agaacggtcg cagttatgcc tttcccggaa
    10681 cctgttgtac cgataattgc tgcatgttgg gttttgaatt cttttatggt tatgtattgc
    10741 ggctggtcat ctttgtttaa tccaatgaaa acgcctttct ttaaatcaat gtaatcgagt
    10801 ggattatatt ctatggggtc gggtaatagc tcttttactt tgcgtacatc ggttcgggta
    10861 tttctctcca gcttgctttt cttaatcatt cgatgtttta ggttgtcaat ttctcctgct
    10921 aaaagtcgtc gggctgcaat atgaaatatt aatcctgctg ccgtaaatat tatggtcatg
    10981 atccatagcg gaactctaaa cagtatgttg ttgtcgagtg acttaaaaaa gtagctcaac
    11041 ccctgaatag ttagcggtga tatggtgccg aatataaaaa agaaaatgga cacacacgcc
    11101 accgacttta accagaatgg ggcgttcttt ctttcctctc ggggaaggtg gaatataaaa
    11161 ggtaatgtaa gacctgcgag cagggataat atgacagggc tgttttgcag ccataacaga
    11221 aaaaaggaaa atgcatcggc aaaggcattc agcctttgca tcaggtggga acccatatct
    11281 attttctccg taggaaaact gacgtgttgg gcgcaaaaga tgggagcagc ttataggagc
    11341 gtaaccacgc tcccatgcga tgctcccata gtatgctccg atgaataggc aaaaggcgct
    11401 gatcgccact ggcgatcaag cccgttatcc gcgctttcag aatacagccg accagtggtc
    11461 gtctgtcctc tgaaacgccc gaatgacggg caacggcgtg tgccattacg ttcgggctgg
    11521 acgccctcac tgcatgacac atggggggaa tgtcccggct ggacgccgag ccaaaacccc
    11581 caaggtcaaa ggcggcacac cgtcttgcgc tacgcgcgac caaccccttt aagacgctgc
    11641 gtttagccta tttttcctcg caagctcgaa aaaatcggaa cgctgcttta aaggggttgg
    11701 tcgcgcctaa tcggtaatat cccccgctgg ggcgggttat attacctggc tgcgacggtg
    11761 tgcggggcaa gccccccaca aaaaacggca cgtgggcgtg cctttttgct cgctggggcg
    11821 gcaaaatttt tgcggctccc cccggcctgc tcaattcgct gtgggcgaat gttcgcaggt
    11881 gggcgcggtt cctgaaaatt agcaatttct tcgaaattga attttctttc cgcgtaccac
    11941 ttcattattt ttcgtcttaa caatttcttc gaaattgaag ccaaaaataa tgaagcctta
    12001 aaatcccgca gcctgttagt gtgtattgta caatacactt ttgttttgtg tgattacatc
    12061 tgctcgcggt atatttcaga cctgcactcc ctttcagctt ccagcgcttc actgtaggac
    12121 gcatcatcaa ttttcgtacc tttgctttcg gcatagcgtc ttaatttatt tgcgtcagct
    12181 tcattaagtt ctgaaagatg atcgcgcaaa agaccgataa aacagctttc gtatatttcg
    12241 taatacagcc cacaacctct gtgggcttct tttgataacg tgtcatatac cagggctaca
    12301 gatattctgt cctgattttt tgagtgtata ttcttaatga ataaattttc gtttatcatt
    12361 tcgaacctct catgtcaaaa tggatatgtc agttatcctt tcactctgcc aaattttgaa
    12421 aggattttgc ttattgattc agggtctgcg gctttacatt cgcctaaaaa ttcttttcgc
    12481 atatcgaatg ttttacccgg attaaaacga tggtgttttt taaaagcgtt gattttaaaa
    12541 aatgcgcgtt ctgctgaact gcattcactc ccaccactat tacccgttgc ttttccgtac
    12601 atgcaaagaa caacttcgca tgggtcagcg gcaaatgaat aaatcggcgt tcctgcggtc
    12661 aggcataata cagccgccgt aataacggtt tttttcatga taaatcctta ttagattgta
    12721 ttacttaaat agattggcga gagaaataaa tggtggtttc tctgcacgtc agacaattaa
    12781 agacaggctg gtattcaata actccttttg cgccattctg gcgaagccag cttttggcat
    12841 taaagaaatc agtaccggat tgtgcagtga cgacaatccg tgttgaatga gcgactgcgt
    12901 aaaatactgc tggagtccat attgtatcgt agaaactttg tgcgctgatt gatgatgtcc
    12961 atttaccgtt aactgtcggg gaagggacta taacgttatt gtcactccat tgaggtaaac
    13021 tggtatttaa tggctctgcg gctttatccc actctacagg aacgggaggc ggtggcgatg
    13081 aacatccggt aactattgcc gccaacatga ttactttaag tttctttatc atgctttcat
    13141 ttcctcaaaa tattgattgc gatggattgg acggaaataa atgtcattca tacggcggac
    13201 atttccgtct aaatcaatgc tcacaatcac gtccaggcta tccagtacct tacgtaacat
    13261 aaatgtatag gggagctggg cgcactgtgg attttcatag caacgggtaa taaacccgtc
    13321 tatagcttct ttcgcagatc ctgcatgaat ggaggtcata ctcccttcat gaccggaacc
    13381 tgttatttta aggaagtccc aggcttccgc accgcgaacc tctgttaata aaattcggtc
    13441 agggttcata cggtaattcc agcgtaaaag gctagccggg gtaacgatag accctttttc
    13501 atctgatgat tctgacggat aaaaaagatg gacgtagttt ttatggataa agaacgtgat
    13561 ttccgggtta tcctcaatgg ttgatatccg aagatgtaca ggaatatacc ctatcaacat
    13621 tttcatatag gtggttttcc cggtactggt ttccccggcg accgcgagcg ttttaccgta
    13681 ctcgacgcat ttctccataa acaaaggaat attttttgta ttgtacaatg caattaattc
    13741 ctcgtcgtgc gtctccgttt tttcctcgcc tgttacccgg ttataaaatc ccgcatcgat
    13801 atatgactga tgcgggattt gtaccttcga tggcttacgg atggtgatag acaccgtatc
    13861 gcgctcacag gccgggggaa caatcacctg gcaccgttcg ccagattcca gcgtggctga
    13921 cagtcccggc ttaatatctt cgatattgtc gccgtggtgc tttgccaggc accgggcaaa
    13981 gttgtagcaa tcctcatagc tcaaaggaac ggcgtgctgc tcccatacgc cgttgatttt
    14041 ggtatacagc tcacctggac ggttgacggc gatttccgtc aggccgggaa gctccagaaa
    14101 gtcgccaaag aaacgctgtt tatagcggtc gagggaaata tttttagcgg tcatctttta
    14161 cctctttcat ccgggaagag agttcatcaa cagaaagaat gaagcgagaa ctggcagcaa
    14221 ccagccaggc aagctggagt aagccagaca cactttttcc taacatgacc gcttcacggt
    14281 cagttaaagg gcgcaggtga taccagattt gatttatggc catccacgcc gtgggcggcg
    14341 caaacagcag aaacagaaaa cccgcgataa gtaatacaag tggtacatca cgcaggctct
    14401 ggccgacaat tcgtgcaatg tacataacca gaatggctag gcgcttcttg ttttctctcg
    14461 tcataacgta ttcctttagc gtttgatttt cagtgtgtaa atgttagaga aatcaatatc
    14521 ctctcccgtg atcaggttga tgatttcgcc ctgattctta tagagcgttg ggggtatatt
    14581 gatgctgttc tccagcgtgg tacgtgccat atccgccata gcctggcggc tgttttccgt
    14641 atagtccgta ttgcggtctt tcttacccgc gctgttggat gcccatgcgc caatatcggg
    14701 gatcatgccg accattaacg cgccgccgaa ccgttcccaa aagtgcgaat ctatccagcc
    14761 atcgacgcca gcctcgccca gctcgccagc cgcgctggta tcaaccagcg ggatatcgag
    14821 ataaggcggc tgacgggtgc gcaacttcgt cgcaatgatg aatgcccgcc cttgcccgtg
    14881 cttcatacct tcttctgcta tggcgcggta aagtaatgat gctgtggtgc ctttctctat
    14941 cagcttcgtg ttgccgctgg cgctccagat gtcagtagtg accgtgcagc gaagtttccc
    15001 cgcccggtca gatacaaagc ggcgatccag tgagcagggg atcgaggtat tttccgggat
    15061 gtacaggtca gggttatagg gtatgcgccg tactggtgcg gggctttgtc ccggcgacgg
    15121 tgcggcggca ttttgcggct gaccgggagg tgtggccgcg ctgctgtcct gttgtccggt
    15181 ggtcgttgcc gatgtcagcg tcatttcctg ctgccgggtt tttacctggt tctgtgtagt
    15241 ggccgaacca tcacggcgaa taaggaagct ggctttgttg agttctggcg gggcggccgg
    15301 agccgcctgc cctgttcccg ctgcgctgtt ctggccgttg tcctgttgct caggctcttc
    15361 ttcggctgta tcggtggaca tgccgagatc gttacgctgt cggtaatcgg ttttgttcgt
    15421 ctgcggggtt gtgtcctgct tcttttcttc cgtaggctgc cacaggatat tgcgataaac
    15481 ccagttaccg ccccatgcca ggaagataag cgcgaccgcg acaatggcaa gaaaggcggt
    15541 tgctttgccc cgtttgcgct ttttcagctt gttaacggcg ggttgtaccg ggtctttctc
    15601 ttcgtcgtcc tgcgcattga gaagctcggc ttccctttta gcgcgggctt cccgctcaag
    15661 cgtggcaata tcatcctcag cgtgtgccgg ggatgcctgc gcttcatccg gcacaggcac
    15721 tttgttttcg tcgttcatat gcatgtttcc tgattttata aaaatataaa actattttta
    15781 taggttaatt gggttccggt ttttccactc tttccacctg ccgggatacg gtggaaccat
    15841 cggcggcgtg taccttgcca aatccgctat tttccagacc cacaacggca ttaccggaac
    15901 gcagtaccag gcgtggcgtc acctcctgaa tcaccagtac ggtgaagtcg cctttcttct
    15961 ggatactgct gttaaccaca tgctctttgc cgttcagctc tttggtgacg gaaggaatgg
    16021 acttagacgg ggagaagccc acgaacgtaa agcggccatc gtcgtaagca aaatcgggaa
    16081 caatgttgaa actgcctttg cccggatatt gggtgtaact ccagttgcgg ggcgtctgcg
    16141 ccgagttcag cgctttctgg acgctggcct gctgctgctt ctgctcccac tcgcgctgtc
    16201 tggccgtcgc ctcccgactg gctttgtccc gttcttcgcc cggatagcga tagctcacct
    16261 ggaaagccgg ttgctgcgtt gatttatcgt caatgacgtt cagctcgacg ttgtacaggc
    16321 gcttactggt gacaaccagc atgttggtat gccagtcccg gctgttcggg gggatcacga
    16381 cctgcgttgt gttgccatcc tctccgggtg ctccctgcgt gagcgcgacc gggcgaacgt
    16441 tgacccggtt tgcatccggc gtggcttccc acgcctcatc aaagccgggt ttggcgctta
    16501 tgaccgcctc atcgttatca aagaccagcg ttgtcataaa gccgggtttg gtgttaacga
    16561 cggtgacgtt ctgcgggtta tagataacct gctgcacgcg ggagtcatag cggcttgcct
    16621 gcggtatggc cgcaccccat gccgcgctgc acatcatcaa tgccagtgcg gtaaacgtta
    16681 actttttcat cattcacctc cccggatttc gcggtcggtc tgccaactgg tgacggtaaa
    16741 gccgaagtag ttgatctcgc gttccgcatc gctcatttcc ttgtcagggt cagaatggaa
    16801 cgtgaaccgg gcatcccaat actcgttgcg ggtgctgtta tcagccagac ggcggataat
    16861 cttcttgtag cggatggtgg cgaccctatc aggggctgtc gcatcggtga tctggttcga
    16921 caggatctcc actttcacgg tgtgcgcccc gttcttataa accttgtccg gagcgttttt
    16981 tccggcgtaa agcgccagat agtcatcgtt tacctgggga gcgttgtaga cctgtaccgt
    17041 ctcgtagtcg tcctgcaaag agggatagac ataccgctca cgcactttga cgtagtttgc
    17101 ggccatcgcc ttttgatagg cttctgttgc ggtcaggctg gttttcgatg tccgggtaac
    17161 gtgttccacc cgtcctgtgt ggttatccac ggtatacagc tcaacgtccg tctgtttcag
    17221 cggcaacatc acgaccacgg cgacgagcgc cagcaccgca atcagcagac ccacaccgcc
    17281 aaccacaaaa ccggcttttg tggcgcgttt atcccgctca atcatttgct gctcaaaggt
    17341 gcgggattca gctattaatt tctgttcact catgactgtt taactccgcc ataatttgag
    17401 gtgtgtttac gggttctccc ttaccgctaa atgcagatgg aagcgtgttc tgggcgcagc
    17461 cagaaagcgc gcacagcaaa gccagcgcaa tcaagcgttt catcaaagat ttcctcaatt
    17521 gaattagaaa tatgaaaata taattatatt ttcataggtt gatatttcgg ggggatggac
    17581 tggagacgtc tggcctagct gttgaacttc ttaaccgcgg attccctcga ctgtgccagt
    17641 cttcgggctt cgttccgggc tatcatctgc tcaatcgcgt atttacgcgc tttagaagcg
    17701 ttatagccta actcacctcc gggcttgccg ggcttaccag cattcgcatc cttccagccc
    17761 tgaatgttgt cctgggcgga acgtttggtt tctttaaccg cccccccggc cagcttcccg
    17821 gcaccaaaag cagcagcacc gatacctaca gcggccaacc cctgcatgga tacggtggca
    17881 cttgccccgg ccaatgcagc agctacttta gcggagatat acaccagtat tgcggcacag
    17941 ataccagcca aacagacctg tgcgcctagt tccacaatat ttgactgtgc agctccggct
    18001 tgagattggg ctaatatttt agtcataaag tttatgacta ttcttaatga taatgctgaa
    18061 aatagaacgg ttaatatcgc tgcaaatata ttttgcaacc agttattaaa catcggtcgc
    18121 aaaaaaccat acataaggca aaaaataaaa ataggcgcag tgatgctaag aagtaagatc
    18181 attatctcag cgaccatcga aacaaaagct gttaaaacca taagaataaa tatcccaagc
    18241 catacataaa attgagcggt catcccctca tctttcacat atgttgaatt atccatatcg
    18301 tgtaatgttt tccccaaaac ttttgcctta tcccataagg tatctaacat ttgccagata
    18361 ttgtcgcctc cagaaaaacc ctcctttaaa ccgttgatgg catcgatcac gccatcgaga
    18421 taaccactga cgttagccac aaacgacagg atgatcgcca tccgcatgat gtcccacatc
    18481 gtgtcttcca tcggcgtgga gagcttacct gctaacgtct gatagcctct ccacatgatg
    18541 tagatggtgg ctgacgcagc ggccagcccc ataaccattg atgaatgttc catcatcaac
    18601 ccactggtag attgagttat cccaccaatt agctgtttat ctataaattc aaatgtaccg
    18661 agttcggcca taaagaacct ctctaatcat ggaattttag cgttggtgcg tttaattgct
    18721 gtatttgata atgggcttca tttgcgtttt tgcagttttc agaagcgtct ccgctttttt
    18781 gacatttttc ataaacaact tttaattcat cagggtgatc acgataccat ttcgttgttt
    18841 tcgcttcttc cttgcatcct gaaataattg ctacaagtga aagggcaacg atggcaataa
    18901 atttattcat aatatttcct tactcatgaa attttaaagt tggcgcgttt aactgctgct
    18961 gattccactg tttaacgcgc tccgcctcaa gcactttttc gcgcttctct gctgctttca
    19021 cgctcatttc ccattgggtg gtaagcgtgt tcagcatgac agattttagc tgaatactgt
    19081 ttgcaaggtc ttgagattct ttcgaatctt tggctaacgc taccctgttg gagaggctgt
    19141 tgatttcccc cagggtctgg cttacctggt tctggatttc gtcggtctgc tcaagctgga
    19201 tagctttatt gatgacttgc tgcttgcata cgttggcata gttccccgac cgttcggcat
    19261 tgcaggtatc gaatattttg tacttctcgt acagcgcgtc gaactgtccg gaggaaccac
    19321 cggagagaag caaatcattc agagcctgtc ccggcttgcg tagtttttcg agatcggctt
    19381 tcaggctttt ggcctggtca acaaagctgg cgatgtctcg aacacctgtg gctgttgcca
    19441 gttggtcttt ataagctcgt atctggtctt tgtagtgttg tgcggtctcc atccattgct
    19501 tggcttcttt ctgccactga cggacatttt ccagtttggc cgtggcatca aatactaaaa
    19561 tacctcccgc ttgtgccaga gggcttgcaa gcgaacatgc gaaaatcagc gcgtaaaagt
    19621 gagttttcat cctatatctc cttttgaatc agagtgcttt agccaggtag gtatcgagcc
    19681 attcatgagg ctgcattcct tcccggtaaa tcgattcgaa tatttcgagg ttgggggcat
    19741 cgccgctcat gactttggtg tatctgccga tacccgacag gtcgagcgtg acacgagcgg
    19801 caaaacgttt ggtatcgcca cgcttgaact gatttttcac cacaacatac tggcgggctt
    19861 gcgggtcaag ccctttcacc acatcgaaca cttccggctc aaacttcata ccgtcaacat
    19921 agtgtgcgcg gtcggcgttg gggttcgccg ccagaatctg tgtcccgcac tgctcaatca
    19981 ctgccggagc gatatcatct ttgatgatct cagccgggga ttgagtaccg acgaccagca
    20041 tcccgttcag cttacggatg gttttcagct tgttataagc aaagtcctta aacaccgggt
    20101 cgcgtaacca cttccagaat tcatccatga aaatgaccag gcgtcgcccg tcaagcaggc
    20161 tggtaatgcg gtacagcaga taaaacgaga ttggcgcaca tacgcttgcg tcgtccagga
    20221 actccgttcc gtcgatgccg aagttatcgc agttgctgat atcgaacgtg tcagactcgt
    20281 tatcaaacac ccagccgaac tcaccgtcct gcgcccattg ggacaaccga atgctaaggc
    20341 cgttttcctg ggcttctttc gtagccggct caggcaaatt ttccagcatt cgggtaatgc
    20401 cgtagacgcg atattgcggc tcgtcattca tcacagcatt aaccgcatca ctcagacggc
    20461 gttcatcgcg tggggagatc gtcgagccgt tgcgggtaca aaggattttc atcagttgct
    20521 tgatgaaatt gatattgcgt ttagttgcag gtaatgagaa cgggttccac cctgtagact
    20581 caccactgat aacccgatag taacggccac ccagcgcccg gatgttcatc tctgcgcccc
    20641 ggtctttatc gaggtaaacg gttgtcagcc gtttggtttt ggcatccggg gagaagctat
    20701 cctcgcgccc gtatttctgc tgcatgatct caaagaacgt catcaacatg gttttacccg
    20761 aaccgttcgt accgaggatg caggtactgg ccgggttctt ctcgttgaat tcgtccttat
    20821 ctgccagtgt gttatgcagg ttgatatagt agccatcgcc tgacggggtg cgcagtaaag
    20881 ccatcgcatc cccccagggg gctttatcgc gcttgcccgg atagaaatta tgaagcgcgg
    20941 ccagctccac aaagttctgg ctactcagct cccctttacg tggccgcagg ttgtagttgc
    21001 ctggcagttg cgccatatac gccgctgaaa gcgatatttc tgccggaaca ggcgttatcc
    21061 cgatgttgtt cagcgcgtta ctgatttcgt tagtatcagc gaccaggctt tccagcgacg
    21121 gggaatacac catgatggaa aaatggtgct ttccgtaagc gattttcccc gatgtcagca
    21181 ggtcaagcgc cacatccaga tccagccgct gggaaacagc atcgtcgtcg gcactcatca
    21241 aaaggcgacg ggtacgctta atggcctttt tcgcttcctc gcgtgacatg caggtgtaag
    21301 actgcgtgat cacgtaatcg cagggcgcat aaagaaggga gtccatcatt cccgtggcgg
    21361 tttcttcgga gaactcttta acctccagac cccggaaata ttgcgtaccg ttggcgtggt
    21421 ttatctgccc ggattcagcc gaaaagaaca acgccgccgc tcccatcacg tcataggccg
    21481 gggtacgggt gacgcgcact tttcgccact ggtgcgttaa cagatattca tagaaagcca
    21541 gttgcgacga aaacaccgcg cttccttcgg taaacgtacc gagaggccgc gcaccatagc
    21601 gcgacagcgc ggtatcgagc gtgcttttta tttccagcat ttccagcaaa gcaccgtcca
    21661 gctcctttag tttctggctg tctttcatcc gtttgcgttc tgctttttca aggctgacaa
    21721 acgggcgata gcagaccgtc aggaacaggc ggttgcgccg gaacagaccg acagaagcgt
    21781 agtaaagacg gctaacctca tcggcatacg ggttgccgga atctttatga agattgtcgg
    21841 taaacgtatt gcggtcgtta tggatataga aagtaacggg cataccttcg aatgagcgga
    21901 ttagcccgtg taactggttc gtcccgaact ggagcgattc gtcagactcg cagtcgaacg
    21961 gcgttcccat caattcccat gtgcagtaca gatcgctttt aggtgagcga accacatgct
    22021 gatgaatgtg cgatgagtac gggatgtact tcttgatggt ggcacactga tttagtttca
    22081 tggcgtcgag aaactccttg atatcaacat catcatgctc aacgggcata aatgttgttg
    22141 cgccaaataa acggtttgct accggctttc ccttcgctct cagccatacg gcaatcagat
    22201 tgaaccgcat cggttcattt ctggtaaggg acttcatcac cagaaattgc acaggaagta
    22261 agaacaggag agcgacggaa gtccagatag caacgatgat ggttacaccc gtgacagcca
    22321 gcaggggcac cattggcacc ccccacagcg ccgccgggcg tttcattgcc ttgagcagct
    22381 ttgccatatg ttcagcctct tagctcatca gcagagcgcc aatttcactg cctccgccaa
    22441 ttaaaatgcc gccaatgatg atgtagccgc actgcgacag gctctcgcct ttccacaggg
    22501 tcttatagcc aatccagatc acggccacgg tgattgtgat agccgccaga ccatgcagcc
    22561 cgctggatac cttctccatg atggtatttg cccggctaaa gccatcagcg aacgccggac
    22621 tggcagcaaa aacgctcatg gccggagcca gaacataagc agatgcacgg ctgattaatt
    22681 cctttacctt actgaaaagt ttcatttttc atcctctttc gttgttacat cgcgatttcc
    22741 gcgcacaacc tgcgcggggt aatagacggt ggccgccttt ttctggcgga gttgagccgg
    22801 aacgccgcga acgatcgccc ccggccacac cacgcgcggg cgcgtcggcg catccactgg
    22861 cgtagctttc agggtggccg ctggggttgc gatatcatcg cgggtgccgg gaacggcata
    22921 gcgggtatcc gccggggagt agccaatccg ctgtacatag ctcgtctttg aaaaagcggg
    22981 ttcctgctgt tgtccggtac tgaaattgcc ggagtagtag cagctcaacg cccttttcag
    23041 ggtgccgccc cgctggtagc agtcggtgat gactttttcg tagacggaaa gattgatgca
    23101 gggattgaaa aggtcagtag cggtgacgcc gtaacgctta aaattggtgc tggttatctg
    23161 catcaggcca acagaatagc ggcgaccttt cgcctcaatc tgattggtga tgctgactgc
    23221 atcctcctta ctttttggca tgtgagtaat aaagccttta tcgccgggtt tccgttcggg
    23281 cttaggcacg atctcagcga tggcataggg gttgtaaccg gactccaccc ttgccacatc
    23341 gagggacgta gaagggtgaa cggtggcggc acactgcata gccaccgcca gaaaagcagt
    23401 ggtggaaagc atggcaacct cagaacagaa aaatggtgat cagccagatg atgaacagaa
    23461 cgaacaacca gccagcgaac gcaagccagg cattgggatt gtctcgattc attgtgctgt
    23521 ctccctgctt taactgacag gagcagcctg cggcgctgcg tcatctttag gttcgaggag
    23581 taccagcttg ccggaaacgg aaataccggg tgtgatcatg atgttcagcc ccggcttgcc
    23641 attgtgtgcg aaggccgcac cgactttcgt ccagtacgta tttttttcgc cgttctcgcc
    23701 gggctgggtt tcctgcgtga cgtacacgtt ataggtgggt tttctcattg cattactcct
    23761 tctgtttctc tgcgagtagc tgccggagca gctcagtacg ggcttgctca ccaaattcat
    23821 ccatcaggaa ctggagaaag gtgcttggcc ggatatttga ttcagatagg tagctgattt
    23881 cacgggcttg ctttgctaac tcctgatagc gcttttgccc gatgtaaata acagccgtgc
    23941 ttgatttgcc tggtacgctt ttcggcatga ggtaaaaact cctatgaaaa aagcaccgca
    24001 gcttatgaag cctgcggtgc aagggaggtc aggttgtggt tagtggtatt ggttctggag
    24061 tagttttgaa aaggctacct ggcgttggag cggagacctt atcagagaga gttcttgaat
    24121 ttttgccagc aaatccgggg caatatttcg gcactcgata gcatcttcat caggattgag
    24181 cgccatcaga cgggcgcatt caatcgctgt tatcactgat ttggggacgg tgcagggtgt
    24241 tgaaccgaag ctgacaaaac catgagcgcc cggaatggat gtgattgccg atgtatgcgt
    24301 tatgttgacg tcaaagcaga ggaaaagata attaggaaac aacaccttct cttcctgacg
    24361 taccgcccgg caatccttac gtggcgttac tttgatatag cgggggcaaa acacctcgac
    24421 atccagtccc tcgactcttc gggtgatgcg ttcaacatcc tgattgttgc agtataaaac
    24481 gtaccattca tacataagat cctccctaat gggcacaagc ccccattttc cgccgtccta
    24541 agttggcgga acttagagcg cgtactcata gcttaactgt ataaatttac aactgttatt
    24601 ttatacagtc atttcggggt gtcaacatca agaactgcta atgataataa catcaatgtt
    24661 catatatgca atacgttttg aatatctgta ctaatcatga gaagcctcta tgtatgtttt
    24721 gaaaaacaaa acaaggtgat aggtcatggt tccaaaacgt ttgaaggaag cgcgagaggc
    24781 cgctggttta tcccaggaaa aattatctga actggtgggt gtggaagggg tcaatcctcg
    24841 gtcaaggatg tctaattatg aagctggacg gtttgcaccg ccgtttgagt tcgtatgccg
    24901 agtggctgat gtgctgggat atccagagta ttacttctat atagtgaatg acgttacggc
    24961 gaagcttctt ctccggatgc atagaggaga acaaaatttg catgaagaca ttgagtttga
    25021 ggcaaaaaaa atggctgaac agcttgaaga cgcaagaaaa ctggtcgccc agcttactga
    25081 atgcctgaaa catcgcccgt aaccctgccg cgcttcgctt gtccaacccc tgatttctac
    25141 acaaaaaatt tgcgtaaaaa tcaggggtta cgtgttttaa atagttgggg tgacggtggg
    25201 ggaatgatct atatttatag tctcaatgtt attgagattc atacaacttt atgatttttg
    25261 gtaagaaaac agagttgctt ttttcgggat ctttggcgag ataattgatc ctaatgtaac
    25321 ggcgtggtaa tttactgacg tttacagtat tcatacgggt gggacgttcc cggtaatagc
    25381 gtccagggaa gggggaagcg aaagctcaac ccctgaatgc aaaaaccccc tgaaatctgc
    25441 tacgaacttg gcgggacgtt cagatattca gggggtcgaa agcaggcgct ggcctgtgaa
    25501 ggattatagc aaatgcataa cctaacacaa cccctcagac tcgccataa
//
