LOCUS       NZ_CP056411             8608 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Citrobacter sp. RHBSTW-00535 plasmid pRHBSTW-00535_3, complete
            sequence.
ACCESSION   NZ_CP056411
VERSION     NZ_CP056411.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148649
            Assembly: GCF_013747275.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00535
  ORGANISM  Citrobacter sp. RHBSTW-00535
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00535
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.9681892140041x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013747275.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:09:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,991
            CDSs (total)                      :: 4,873
            Genes (coding)                    :: 4,720
            CDSs (with protein)               :: 4,720
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 44 of 153
            Pseudo Genes (incomplete)         :: 119 of 153
            Pseudo Genes (internal stop)      :: 33 of 153
            Pseudo Genes (multiple problems)  :: 36 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Citrobacter sp. RHBSTW-00535"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00535"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742654"
                     /plasmid="pRHBSTW-00535_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(1003..1608)
                     /locus_tag="HV232_RS24810"
     CDS             complement(1003..1608)
                     /locus_tag="HV232_RS24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            1703..4600
                     /locus_tag="HV232_RS24815"
     CDS             1703..4600
                     /locus_tag="HV232_RS24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_023307208.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEVMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4653..4880
                     /locus_tag="HV232_RS24820"
     CDS             4653..4880
                     /locus_tag="HV232_RS24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230090456.1"
                     /translation="MFGFSPGELMNLSPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(4928..5116)
                     /locus_tag="HV232_RS24825"
     CDS             complement(4928..5116)
                     /locus_tag="HV232_RS24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5407..6039
                     /locus_tag="HV232_RS24830"
     CDS             5407..6039
                     /locus_tag="HV232_RS24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001567369.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     gene            complement(6068..7471)
                     /locus_tag="HV232_RS24835"
     CDS             complement(6068..7471)
                     /locus_tag="HV232_RS24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISKpn21 family transposase"
                     /protein_id="WP_001567368.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="ISNCY-like element ISKpn21 family transposase"
     gene            7677..7886
                     /locus_tag="HV232_RS24840"
     CDS             7677..7886
                     /locus_tag="HV232_RS24840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032201350.1"
                     /translation="MTKFEAFKDQITNAAKASFPEWVTFEYENDFPGYNESFVYESVCA
                     IKKMGELEVRNNADRYFSVKFISA"
                     /name="hypothetical protein"
     gene            complement(7942..8322)
                     /locus_tag="HV232_RS24845"
     CDS             complement(7942..8322)
                     /locus_tag="HV232_RS24845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032201349.1"
                     /translation="MKKITSLLLLGVFGLTGCAQNVSPQVAAQAQAPIYCENKQQCDAY
                     WTKAKAWIAMNSGWKIQMADETVIATYSPIGNSAILGYQVVKTPIGGERHEIVVQTACA
                     NMFGCIPNAIEQTAKLKNYIKN"
                     /name="hypothetical protein"
     Typing          complement(21..510)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.316"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gactaactcc tctaaacagt tatcagtggc tgctgccagt ggcgcgttgt cgtgtctttc
       61 cgggttggac tcaagtcgat agttaccgga caaggcgcag cggtcggact gaacgggggg
      121 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
      181 aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
      241 gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc ctgtcgggtt
      301 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
      361 gaaaaacggc ttcgccgtgg ccttcttgct tccctgttaa gcttcttcct ggaagatgcc
      421 aggaaatttc cgccccgcca gtaagccggt accgctcgcc gcagccgaac gaccgagcgg
      481 agcgagtcag tgagcgagga agcggaatat ctccgggctt atctttggca ccgtacgcgc
      541 catagctgca tttttttata cgatgcagga tagggctaac gccgcaaaat gacgtcatcc
      601 gacatcattc cgccgcgttc gcccagtgac ctacagtcgg ctctggggtg tcggggctgt
      661 gccctgacca ggcgttttgg agtatcgcca ccgtggtggc ggtgctacaa aacatatcct
      721 gtcccttcct tggcatctct gaagtttagc ctgtatacac tccgctagcg ctacgtgact
      781 ggttcagggc tgcgccccga cacccgctaa aagcactgac gcgcctgcgg cttgcccaac
      841 cccgcgccgt ccgggaaaga atttcccgat cgacccgacg tttctgttta cctgtgatgt
      901 taacgtttgg atctttttcc cgggtttacc tattttcttc taatgaagcg ttcacagctg
      961 acgttaatag gggtcggttc cggctgaggg cgaaatgaca ccctaagcgt tagctctgtg
     1021 tcgttgcacg atgtcagcga cggtattctt gctgataccg agctcgcgtg cgatccagcg
     1081 atagctgcgt ccctcggccc tcatcgcaac caccttaggc aaaagtcggt ctgattttgg
     1141 tcgcactccg gcctgacgac caagcctctt accacgtgcc ttcgcaacag caaggcctga
     1201 cttgacccgc tcgctgatga gatcccgctc aaactccgca atgccggaaa gaaacgtcgc
     1261 cagcattcgt ccatacggcg acgaaagatc gaacgccatt ccattcatgg ctatcacgga
     1321 aaccttccag ttctccagtt cacgtagcgt attgagcaga tcgagcgtcg agcgccccca
     1381 ccgggaaagc tcagtgacca ggattgcatc aatttgtctg gactgggcaa gcgccaggac
     1441 tttctttcgc tcggcccggt cgagtttagt tcctgaacct gtttccttaa atattcccac
     1501 cacgtcgtag ccggcacggc cggcgaaggc tcgcagatca aattcctggc gttcacaaga
     1561 ctgatccgct gttgaaaccc ggcagtaaat ggcggcacga tgtcccaatt gaaccctcct
     1621 ggatttttgt atcggaacgc cctgatttat atgggctggc tgttgtccaa aacagactat
     1681 acttcaaaag ggacgaattt gtatgtcacg acgccatatt ttcaccgaac ggcagcgagc
     1741 agcgctgttc gatctgccca cggacgaact gtcgctactg aagttctaca cgctgggcga
     1801 tgatgacctg gaaaacatta ggcagcgccg cagaccggaa aacaggattg gctttgccct
     1861 gcaactttgt gccttacgat atccgggccg tgcactggct cctggtgaga tgatcccgcg
     1921 tgaagtcctt tccttcgtcg gtgctcagct tggagttccg gctgatgcgc ttctcactta
     1981 tgccacacgg cgccaaaccc gtcagcagca catggacacg ctgcgcgaaa tttacggcta
     2041 caagaccttc acgggccgtg gtgcccgtga tctgcgggag tggactttcg gccaggccga
     2101 agatgccaga tcaaacgagg atcttgctca tcgttttatt gtgcggtgtc gggaaacttc
     2161 caccattctg cccgcagtat cgacaatcga gcgcttgtgc gcggatgctc tggtcgccgc
     2221 tgagcggcgg attgaaacgc ggattgcgga aaatttaaca gcggatgttc gcgatcacct
     2281 ggacaaactt ctgagtgaaa tgctcgccgg caatatcagt cgtttcatct ggcttcgcaa
     2341 cttcgaggtt ggtaacaact cggctgctgc taaccgtttg ctcgacaggc tcgaatttct
     2401 gcgtaccctg aatatcaatc atagtgcttt ggccagcata cctgcccatc gcattgcccg
     2461 gctgcgtcgg cagggtgaac gctacttcac cgacggtttg cgtgacatca cttcggaccg
     2521 ccgctgggcg atccttgccg tctgtgttgt ggagtgggaa gcggcgattg ctgatgccat
     2581 agtcgaaacc catgacagga tcgtaggaaa aacctggcgg gaagcgaagc gccagcatga
     2641 cgaaacaatt tccggctcta aagccacact cacggatacg atccgtacct tcaccgcgct
     2701 gggagcttcg ttgcttgagg cccgcagtga cggaaccccg ctggagatgg ctgtcgccag
     2761 ttcggttgca tgggaccggc tcgctcaact ggtagcgaca gggactcaac tcagcaacac
     2821 gctagccgat gagcctcttg catatgtcgg gcagggatac catcgctttc gtcgttatgc
     2881 gccccgcatg ttgcgctgtc tgaagctcga agccgcgccg gtcgccggac cattggtagc
     2941 agcagctttg tcgatcggag agatgaaagg tgttgcatcg ccagaaaggc gtttcctgcg
     3001 gcccagctcc aaatggaacc gtcatttacg agctcaggaa aaaggagata cccgtctttg
     3061 ggaagtggcg gtactctttc acctccggga tgcttttcgt tccggagatg tctggctcgc
     3121 tcattcgcgc cgctatggtg acctcaagca ggtactggtg ccgatgatcg cggcgcagga
     3181 aaatgcaaaa ctggccgtgc cttccaaccc acaggattgg ctggcagaca gaaaggcgcg
     3241 actcacgatc gctcttaagc ggctggcccg ggctgcccgt aacggcacta ttccgcacgg
     3301 tagcatagaa gatggaacgt tgcggatcga caggttgaca gcagacgtgc cggatggtgc
     3361 cgaggcactc atactggatc tgtatcgccg aatgccgtcc gttcggatta ccgacatgct
     3421 gcttgaagtt gatgcagccc ttggtttcac agatgcgttt acccatctga gaaccggggc
     3481 tccatgtcgc gaccggatcg gtctgctcaa cgtcctgctc gctgaagggc tcaatctggg
     3541 cctgcgtaag atggcggaag ctacaaacac gcatgattac tggcagctct cacgccttgc
     3601 ccgctggcat gttgaaagcg aagccatgaa ccaggcattg gcaattgtgg tggccgcgca
     3661 gggtaaactg ccgatgtcac gcgtctgggg gatgggcacg tcagcatcga gcgatggtca
     3721 gtttttcccg acagcgcggc atggcgaagt catgaacatg gtcaatgcca aatatggttc
     3781 tgttcccggc ctcaaagcgt atactcacgt aagcgaccag ttcgcgccat tcgcttgtca
     3841 gtcgatcccg gcgaccgtga gcgaggcacc gtatattctc gatggactac tgatgaacga
     3901 ggtcggtcgc catgttcgcg aacagtatgc cgatacagca ggattcaccg accatttgtt
     3961 cggagccagt agcctgctcg gctacaatct cgttctgcga atcagggatc tgccatcgaa
     4021 gcggttgtac gtatttaatc ccgatacgac ccccagggag ttacgcaagt tggtaggtgg
     4081 aaaagcccgg gaggatctta tcgttgcgaa ctggcctgat attttccgtt gtgccgcgac
     4141 gatgaccgct ggcaaaatca ggcccagcca actcctgcgc aagctcgctt cttacccacg
     4201 acaaaacaac cttgcagttg cgcttcgtga agttggtcgt attgaacgga cccttttcat
     4261 tattgagtgg atcctggata cggacatgca gcggcgtgct cagatcggtc ttaacaaggg
     4321 agaggcccac catgcgctca aaaatgcgct ccgtatcggg aggcaggggg aaattcgcga
     4381 tcgcacgaca gaggggcagc actaccgaat cgctgggctc aatttattga ctgcggtgat
     4441 catttactgg aataccgtcc atcttggtca tgccgtcacg gagcggcgga acgaagggtt
     4501 ggatgttccc cctgaatttc ttccccacat atccccattg ggctgggcgc acattctact
     4561 gactggcgaa tatctttggc ccaaggaacc gaaagcttag ggtgtcattt cgccctcagc
     4621 cggaaccgac cccttaatag aggggtgctt tcatgtttgg tttttcacca ggagaactta
     4681 tgaaccttag ccccgatgaa cgtctgctct ttgtgcggat gatttccgct atgcttcgcc
     4741 gttcgggcgg tgatgcgggt gctgttatgt ttgaggcgta tcgccatatt gtgtccgata
     4801 ccaatcaagc tcgtcgttcc tacatgctcg acctgctcga aagcgtcagg catgactatg
     4861 tacacggtgg atatacatag ttgccctgga tacgcttttc gtctaagtga gcgttctttt
     4921 cgtctcttta ttcgccgtcc tgtataccga agcgtgcgct cagcttctga taaagcgctt
     4981 ctgcctgttc gtgcattcgc tcacagtcgg ctgcctcgtc atcaagatcg agcacgtcga
     5041 gtttttcgag cagcagcagc gactgcgccc ggataaattt tgccatgttc agtgcggctg
     5101 attcctgttt tgtcatacgg ctttttcccg gttttatggt gatcgcagac tgaaatctta
     5161 cttcattcac ttgagtgcat agccatgcag gtacgcagaa atcatcaggg gcgttttttc
     5221 tcgatatggg cacagaaagg tgtatgtttc aggccggagt cctcagaaac gccctgaggg
     5281 cgcctgaggt tgtcaaggct caattgaaat gtccgttatc cagctcaatt aaaatgacca
     5341 ctttgatctc tcattctctt tactgataga ctttcctccg actgaaacaa caggatgatt
     5401 gagcccatgc ttcgatacga gttaacgccg aacaatgcag gttttatact gtggggagat
     5461 tcagaagccc tgaatgaatt acatgaactc attcattaca tcgtggatga aagcccactg
     5521 attaaagtta aagacggatt tatgttatcc cttgcctatg atattcgtaa agcacgggaa
     5581 ggtaatcgtc gtgttgagca acatcagtat gatcaacatg atacatataa gctttatggt
     5641 gttgagcttt tatggcctct ggtcctggta cagtcctcaa tactcagaaa ctcaatgggt
     5701 tatattcaga cagacaaaaa ccagctgtct gtcatgtatg cctttgaata cctgatagaa
     5761 tcagcattaa cagagtctga gagaacaacg tcgaatgata ttatgctaac agtaaaatat
     5821 gcatcagact ctgattttaa tttcattgag gataatattg acagcaggtg ctgctatttt
     5881 atcagcctat ctccggagca aagaaaaaag cagttaatca gtattgttcg ttcttttcat
     5941 tcattatggg gtaagtatgc ccgtgaaaag caggacataa agatgctgaa cgaaatgaat
     6001 aatacatcat gggtctggcc ggacaatatc aactggtgag caaccactgt ccggccagtg
     6061 agtaccatca gcgggctctt ttaccaaaaa tgtcctctga acgtgactga agctgtttga
     6121 gtgcttcgag catgtcatca ttatccagtg agcgctggga tttcttccct tcctgctttg
     6181 gccgtcgtcg ggaagggcca tctccagcgg gaattgactg agagcgcgtg ttatcccgct
     6241 tgctctgcac cagactgata aactccaggg ttcggccaag acgcttgtta tcgacaatcg
     6301 cgccctggtc gatttctgac agtcggtcgt aggtagagta gggaagtagc gtaccgttca
     6361 ggcgcagctc ttttctgcca tcaggatagt gatacacatc gatatattta cctattgcac
     6421 ggcgactcag ttcgctgtct tcaatcaggt acaacatttt atcatattgt atcgtcaacg
     6481 attttgagac tttacgtttt tcacgaacag tgaaaataag ccccaggtcc tcatcatgtt
     6541 ctacagcacg gtgtacgtca aaatcatgtc gcggtacttt gccaaaacgg cggttatagt
     6601 cagccatata ggcctcagcg aagtcatttg cagcctccat tgaacaaatg ccctgtaacc
     6661 gcagctcttt gaccagacga tcctgtaaag tgaggtgagc tcgttctaca cgccctttgg
     6721 cgggactggt ttctgcacag atagtctgga tgttcagttc atgcatggct cgcccaaact
     6781 gagtatgccc gtctccgcct gtggcgtgtt tattgttaac acgaaaaaca ccggctttat
     6841 cgctgtacag tgccagcggt ttaccatgct tatcgatata gcgccgcgtg gcttcgaagt
     6901 aagaaaacgt ggactccgat ttaacaaaca acagttccat cagtttgctg gttgcatcat
     6961 caacatagac cagcgcggtg caggccgggc cacggccttc aaaccagtcg tgatcacagc
     7021 catctatttg tatcagctca ccagtacacg gacgccggta ccgtggttga gggatccttg
     7081 cggcacgttg tttacgggga acccataagc cagcccgcac catgatgcgc cggacagttt
     7141 ctttgccaag aaacagtccg tggagttctt cgagcttttc acgcgccaga gtcggaccga
     7201 aatcagcata acgcgtcttg atcagttcca gagcctgatc tgcgagcccg ggtggcaact
     7261 ggcggttacc acgcatgcca catcgtctgc tggccatacc aagcggtccg ccttcacggt
     7321 aacgggcaag aagtctgcgg cattgcctgt cgctgatacc gaggtgctcg gccgcacggc
     7381 gcgttgtgat gcgacgttca atgacgtcct gtataatctt gatccggttg atctctttca
     7441 aagtaaacac tcctgagctt tctgcgctca tgatatgcct cccggtagtt taaacggacc
     7501 agtgaagctt accatagcgc ggacatctga attgagccac aggcggacat tactattgag
     7561 ccattacaga ggtggtgcgg gcaacggatg ttatggtaaa ttggtgatct catcatgcta
     7621 attctttctg tggtaaaatc tctctgagcc acccggttct tacataggat atatccatga
     7681 ccaagtttga agctttcaaa gatcagataa cgaatgccgc taaagcatct tttcctgagt
     7741 gggtaacgtt tgaatatgaa aatgattttc ctggatataa tgaatccttt gtttatgaat
     7801 ccgtctgtgc tattaaaaaa atgggtgagt tagaagtaag aaataatgcg gacagatatt
     7861 ttagtgtgaa gtttatatcg gcctagcatg tttgattgtt tcggaaagcc cgataaacgt
     7921 gggctttcat gaatataatc atcagttttt tatgtagttt tttaattttg ctgtttgttc
     7981 gatggcatta ggaatgcagc caaacatatt ggcgcaggct gtttgaacga caatttcgtg
     8041 cctttcccct ccaattggag tttttaccac ttgatatcct aaaattgcac tattaccaat
     8101 aggtgagtac gttgcaataa ccgtttcatc cgccatttgt attttccagc ctgaattcat
     8161 tgctatccaa gcttttgctt tagtccagta agcatcacat tgctgcttat tttcgcaata
     8221 tataggcgct tgagcttgag ctgctacttg tgggctgaca ttttgagcgc agccggtcaa
     8281 gccaaagact ccgagtaata atagagaagt tatttttttc attttaagcc tctgttgttt
     8341 attgtttttt atgtgctgat tttaacatta tctatacgta ggaaagaaga aagtaggtgt
     8401 ggttaggttg caaaagaaaa atgatcttct tgagatcctt ttgagcagcg cgtaatctcc
     8461 tgctctgtaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagagatc
     8521 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaatct gtccttccag
     8581 tttagcctta accggcccat aacttcaa
//
