LOCUS       NZ_CP056390             3515 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00610 plasmid pRHBSTW-00610_6,
            complete sequence.
ACCESSION   NZ_CP056390
VERSION     NZ_CP056390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148664
            Assembly: GCF_013735155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00610
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.9533752167854x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013735155.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:20:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,390
            Genes (coding)                    :: 5,246
            CDSs (with protein)               :: 5,246
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 60 of 144
            Pseudo Genes (incomplete)         :: 80 of 144
            Pseudo Genes (internal stop)      :: 29 of 144
            Pseudo Genes (multiple problems)  :: 22 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00610"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00610_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3216..3515,1..198)
                     /locus_tag="HV247_RS27695"
     CDS             join(3216..3515,1..198)
                     /locus_tag="HV247_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            205..420
                     /locus_tag="HV247_RS27335"
                     /old_locus_tag="HV247_27335"
     CDS             205..420
                     /locus_tag="HV247_RS27335"
                     /old_locus_tag="HV247_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(834..1025)
                     /locus_tag="HV247_RS27340"
                     /old_locus_tag="HV247_27340"
     CDS             complement(834..1025)
                     /locus_tag="HV247_RS27340"
                     /old_locus_tag="HV247_27340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071008427.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLTK"
                     /name="hypothetical protein"
     gene            complement(1445..1576)
                     /locus_tag="HV247_RS27875"
                     /old_locus_tag="HV247_27345"
     CDS             complement(1445..1576)
                     /locus_tag="HV247_RS27875"
                     /old_locus_tag="HV247_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072072138.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRISRAS"
                     /name="replication initiation protein"
     gene            2197..2538
                     /locus_tag="HV247_RS27350"
                     /old_locus_tag="HV247_27350"
     CDS             2197..2538
                     /locus_tag="HV247_RS27350"
                     /old_locus_tag="HV247_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181491529.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKRPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2747..>3175
                     /locus_tag="HV247_RS27735"
                     /pseudo=""
     CDS             2747..>3175
                     /locus_tag="HV247_RS27735"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          1939..2112
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1464..1972)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.706"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          4..536
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.15e-105"
     Typing          2529..3514
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaccggatag cggcgaacct ggcggaaatc aggcagcaga gagatacgct gtcgaaactg
       61 cacatgcaga cgtggggcgt gacctatctg gagaaccgca acggacggtt tctggtgctg
      121 ccggagggga tgaaagccga aacgggccgg acggtggaca acaagacgcg gaacgcggtg
      181 aagctggtac gggagtagag cagaatgacg gaactggaaa aacaattgct gagcgcgtta
      241 gagcagctac agcaggacta ctcgagaagg ctggacgagt gggagagcgc cttcgaggaa
      301 tggcggaaga tgtctggtct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgtcg
      361 ggcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttatc gacaggctga
      421 atgagattga gcaacagcga tacgggcaaa caacggaaaa ggaaccagag aagacagcag
      481 atctggtacc ggaacggcaa cgcacattat ctcatggtga cagggagtat ggagactgat
      541 aaacgcccgt ctcaggtggc ctgagcgtga cggggatcat ggccattttc tgcgatttca
      601 gcacacagag agtctttttt aaccgaaatc accggctatg cagtgatgtg aataagtgat
      661 gcatatgcgt aaaataaatc aggtgcgttt ctggtggttt tcggtgtact tgccaccacc
      721 ttttgcccgt cagccatcag aaacgccctg agtgcctccg gcgcggtgcg ggtaagaaga
      781 cgttatggta aatcggtggc tttatttcga tccttcatgc ttcaggggct gtatcacttt
      841 gttagtttta gttttgtggt ttcggcttgg attccgagaa gatcgtggag gttattgttg
      901 atagcaaatt ttatatcttc aaaacttgca tcaatcatga gtccctcatc cacacctcgt
      961 aaaaaaattt ttgtttttga gttaccacat gattttatta agataatttc attttttttt
     1021 aacatgacat tgtcattgtt tgttgtcgtc agatgaatca ttatcgttgt ttttgtgtgt
     1081 gtttgttaag gctgctaaga ttattcctaa ccactgattt tgtacatatc atcaaaaaaa
     1141 taaaaaaact aatgccctgt ggtgggtatg taacaaatca cggggagcgg gtcagaacta
     1201 cgccaaaact ttgaatgatc gggtggcagg ctaaatgaaa tcttgagatt cattcagcct
     1261 cgtcgtaatc tcttgctctg taaacgaaaa aaccgccttg cagggcggtt tttcgaaggt
     1321 taagtcagtt ggggaactgc ttaaccaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ccagtgtagc cgtagttagg ccaccacttc aagaactctg tagcatcacc
     1441 tacattaact cgctctgcta atcctgttac cagtggctgc tgccagtggc gctttggcgt
     1501 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcag tcggactgaa
     1561 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     1621 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     1681 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     1741 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     1801 agcctatgga aaaacggctt cgccgcggcc ctatcgcttc cctgttaagt atcttcctgg
     1861 catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga
     1921 ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct
     1981 gacgcgccgg tgccgccttt tttctcctgc cacatgaagc acttcactga catcctcatc
     2041 cgtgccaaca tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
     2101 cgccccgaaa ccccactgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg
     2161 ctttcccgtc cgtcacggcg ttatcaggag gcgattctgg cagataaacg caacaagatg
     2221 ctcacgatgt gggtgacgga agacgaacac cggcggttgc ttgagcggtg cgacggaaaa
     2281 caactggcgg cgtggatgcg ccagacctgc cttgatgaga aaccgacacg cgccggcagg
     2341 cttccctcga tttcgcccgc gctgctgcgt cagcttgccg gcatgggcaa taacctgaac
     2401 cagatcgccc ggaaagtgaa cgccggtggt gccggtcatg accgggtgca gatcgtggca
     2461 gcgctgatgg cgatcgatgc cggtctggaa cggttgcggc atgccgtact ggaaaaaagg
     2521 ccggacgatg attgttaaat tccatccccg gggtcgcggc gggggcgccg gtcctgtgga
     2581 ttatcttctg ggtaaagacc ggcagcgtga aggtgccagc gtcctgcagg ggaagccgga
     2641 agaagtccgg gagctgattg atgcgtcgcc gtatgtgaaa aaatatacct ccggggtgct
     2701 gtcattcgcg gaggcggacc tgccgccggg tcagcgtgaa aaactgatgg ccagctttga
     2761 gcgggttctg atgcccggac tcgataaaga ccagtacagc attctgtggg tggaacacgc
     2821 agacaagggt cgtcttgagc tgaattttct gatccccaat accgaattac tgacggggaa
     2881 acgcctccag ccgtattacg atcgtgccga ccggccgcgc atcgatgcgt ggcagacagt
     2941 ggtcaacggc aggctggggc tacatgatcc gaacgacccg gagaaccggc gggcgctggt
     3001 cacgccttca ggactgccag aaacgaagca ggaagccgca gagacgatta cacggggttt
     3061 gctgatgtta gcctcatcag gagagctgaa aacgcgtgat gacgtcacag gggcgctcac
     3121 agcggccggt tttgaggtgg tcagaaccac aaaaaacagt atcagcattg ccgacccgga
     3181 cggggggcga aatatccgac tgaggggagc catttatgag cagtctttta acggcctcga
     3241 gggacttcga ggagaaatcg aaagcacagc agcagcttac cgaagagagg ctgaaagccg
     3301 cattcagcaa gcacgagaac gttgtcaaat cggaactgaa cgcaagcgcg aagagaatca
     3361 acgacgccat cagcgcccac gaacagggga tgaacgcggc gatgcagtcg aaccggctga
     3421 gcgtgctgcg aatggtcggg cggacgtggc tgaccatcac catggtgtca gggctgctgt
     3481 tcgccagcct gagcggggtg ctgtggtacc agggg
//
