LOCUS       NZ_CP056389             4426 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00610 plasmid pRHBSTW-00610_5,
            complete sequence.
ACCESSION   NZ_CP056389
VERSION     NZ_CP056389.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148664
            Assembly: GCF_013735155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00610
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.9533752167854x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013735155.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:20:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,390
            Genes (coding)                    :: 5,246
            CDSs (with protein)               :: 5,246
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 60 of 144
            Pseudo Genes (incomplete)         :: 80 of 144
            Pseudo Genes (internal stop)      :: 29 of 144
            Pseudo Genes (multiple problems)  :: 22 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056389.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00610"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00610_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4420..4426,1..209))
                     /locus_tag="HV247_RS27295"
                     /old_locus_tag="HV247_27300"
     CDS             complement(join(4420..4426,1..209))
                     /locus_tag="HV247_RS27295"
                     /old_locus_tag="HV247_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            543..1004
                     /locus_tag="HV247_RS27300"
                     /old_locus_tag="HV247_27305"
     CDS             543..1004
                     /locus_tag="HV247_RS27300"
                     /old_locus_tag="HV247_27305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138058728.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYHDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(1260..1370)
                     /locus_tag="HV247_RS27690"
     CDS             complement(1260..1370)
                     /locus_tag="HV247_RS27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063858821.1"
                     /translation="MHEPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            2018..2341
                     /locus_tag="HV247_RS27310"
                     /old_locus_tag="HV247_27315"
     CDS             2018..2341
                     /locus_tag="HV247_RS27310"
                     /old_locus_tag="HV247_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2550..>2984
                     /locus_tag="HV247_RS27730"
                     /pseudo=""
     CDS             2550..>2984
                     /locus_tag="HV247_RS27730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3019..3510
                     /locus_tag="HV247_RS27320"
                     /old_locus_tag="HV247_27325"
     CDS             3019..3510
                     /locus_tag="HV247_RS27320"
                     /old_locus_tag="HV247_27325"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3517..3732
                     /locus_tag="HV247_RS27325"
                     /old_locus_tag="HV247_27330"
     CDS             3517..3732
                     /locus_tag="HV247_RS27325"
                     /old_locus_tag="HV247_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1732..1905
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          complement(1204..1808)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2019..2335
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="8.540000000000001e-63"
     Typing          2332..3824
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctccgcgtt ctctgagaat tttcattgcc atgctttttt ctgtgttact ggctgagccg
       61 aaaaaaccat cagatttaat gaggcgcttt agttcttcac tgcttttgct ttctaagcgc
      121 attttggttg cctggagttc atgagctttt tcatccaaag cgttagctgc gccttttgca
      181 attttaccgg ccgtttccca aaatcccatt taaccactcc ttaatgctta tccaattgga
      241 cttgttgtga tccgacgact ccgacattta gggcaataat caggcgtgcc ataaccagta
      301 cgactggtcc actcatgctt acaatccaaa cacttagccc ggtagattgt cggtgccatt
      361 gaatactcca ttgattttaa aaatatttgt atagtgcact aaaattatcg gcagtttttc
      421 tcggagcttg agaggtagat aaccctaagc tgatatgttg cctttatgat ggttttcgtg
      481 tgccaaggtt agctaggtaa gggatttact gtgggaagtg ctggttcgcg aagtgaagcg
      541 ttatggtaaa tacatattat ggttatttat taggagtaac tctgggagag tgggctatgg
      601 gatcagatgt tcaatggcag atagataatg gttatttggc gcaagttatg ggggattttt
      661 cgacagagtc gcctagcttt gaaatgccaa tgtttgggcg atattttgat acctttcaag
      721 ctgctataca tgttgcaaaa gctttcccag ggagtacgtt gcagccatca gagtacgaag
      781 ataaaactgg gatggtaata cgtcattatt atgtggattg tcatccgtat catgatcgac
      841 ttcctcctga aaaaagcgat gattttcagt atgatgaata cccttctgct attcatgtgg
      901 ctgcgtgtat tgatcaagcc agcaaagaac tcgacgaact attgtttggt ggtggatatg
      961 gggaaaatgg tgaggggcat gcaagcccga taattttata ttagtgtgag ggggcggagg
     1021 gatattatcc aatttgtaaa atgatcttct tgagatcgtt ttgctctgcg cgtaatctct
     1081 tgctctgaaa acgaaaaaac cacctgggga ggtggttttt cgaaggttca gtaagttggg
     1141 gaacttctga accgtggtaa cagtcttgtg cgagacgtgt caccaaatct gtccttccag
     1201 tgtagccgta gtccggccac cacttcaaga actctcgata catctctcgc acatcctgct
     1261 taccagtggc taccgccaat ggcgattttg tcgtgtcttt ccgggttgga ctcaagttga
     1321 tagttaccgg aatgggcgtg gtagtcggac tgaacggggg gttcgtgcat acagtccagc
     1381 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     1441 taacagcgga gtgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     1501 gccaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacca ctgatttgag
     1561 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg
     1621 gccttctcgt ttccctgtta agtctcttcc tcgcatctac caggaaattt ccgccccgct
     1681 cgtaagccat ttccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1741 aagcggaata tatcctgtac cacatattct gctgacgcac cggtgccgcc ttttttctcc
     1801 tgccacatga agcacttcac tgacaccctc atccgtgcca acatagtaag ccagtataca
     1861 ctccgctagc gcttcgtgac tggttcaggg ctgcgccccg aaacccgcca aaagcctgac
     1921 gcgcctgcgg cttgtccaac cccgcgccga ccgggaaaga ggttcccgcc cgtcccggcg
     1981 ttatcaggag accggtttgt cagagaaacg cagcaaaatg ctcacgatgt gggtgaccga
     2041 agatgagcac cgccgtctgc tggaacgctg cgacggtaaa cagctcgcgg cctggatgcg
     2101 gcagacctgc ctggacgaaa agcctgcgcg caccggaaag ctcccctcaa tctcgccggc
     2161 gctgctgcgc cagcttgccg gcatgggcaa taacctgaac cagatagccc gccgtgttaa
     2221 cgccggcggc ggcaccggcc atgacagggt acagattgtg gcggcgctga tggccatcga
     2281 tgccgggctt gagcggctgc ggcacgccgt tcttgagaaa ggaacggacg atgatcgtta
     2341 aatttcatcc acgcggtcgc ggcggtggcg ccgggccggt ggactatctt ctgggtaaag
     2401 accggcagcg tgaaggtgcc agcgtcctgc aggggaagcc ggaagaagtc cgggagctga
     2461 ttgacgcgtc gccttacgtg aagaaataca cctccggcgt cctgtcgttt gcggaggccg
     2521 atctgcctcc cggccagcgg gaaaaactga tggccagctt cgagcgggtt ctgatgcccg
     2581 gacttgataa agatcagtac agcattctgt gggtggagca cgaggacaag gggcgtctgg
     2641 agctgaactt tctgatcccc aataccgagc tgctgaccgg caggcggctc cagccgtact
     2701 acgaccgcgc cgaccggccg cgtatcgatg cctggcagac catcgtgaac ggcagactgg
     2761 ggttgcacga cccgaacgca ccggaaaacc gccgggcgct ggtcacgccg tccggactgc
     2821 cgaaaacgaa gcaggaggcc gcagaagcca tcacgcgcgg actgctgacc cttgcttcgt
     2881 ccggggagct gaaaacccgt caggacgtca ctgaggcgct ggaacggtca ggttttgagg
     2941 tggtgcgcac cacgaaaagc agtatcagca ttgccgaccc ggacgggggg cgaaacatcc
     3001 gactgaaggg agccatctat gagcagtctt ttaacgctgg cgaaggactt agagcagcaa
     3061 tcgaaagcgc agaagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag
     3121 cagtctgtca gagcggaact gagcgcaagc gcgaggagaa tcagcgacgc catcctcgca
     3181 cacgagcaga gtatgagcga ggcgatggag aaaaaccggc ggagcgtact gcggactgcc
     3241 gggcggacat ggctcaccat cctgatggtg tcagcgctgc tgatcggcac gagcgggagc
     3301 atactgtggt ggcaggggca gcagataacc gacaattaca cgcatcttcg tcagcaggaa
     3361 gataccctgg cgaaaatgac cgcccggacg tggggagtgc gctatcagga gagcagcgac
     3421 ggccggaggt ttcttatcct gccgccgggg atgcaaaccg aagccatccc ttacgacggg
     3481 acgacatgga tccgcctgaa acaggagtag cggaaaatga cagagctgga aaagcagttg
     3541 ctgagcgcat tagagcagct acagcaggac tactcgcaaa ggctggacga atgggagagc
     3601 gccttcgcgg aatggcggag catgtgtggg cttatgcaac gggagaacgc ggtgctgaac
     3661 gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa gcgagcagct gcgccgcttg
     3721 tccgggaact gaatgccatc gagctccggc agagggagaa tgagtatcag aaaacactgg
     3781 agctggagcg ccagccgcag aaaacctacc gcgggccgac actttaaacc atccaggcag
     3841 ctgacaaccc gcacgaagag gggctcttac ttctcccgct aaataggttc gtgatcaaaa
     3901 aggtcacgac ttattcagga gcgggaaatg aagctgttaa atcttattga atcattgctt
     3961 aactgcctgt atctggcggc ggagctgtgg gactggctgg agaagcattt gtcggttttt
     4021 aaggcgttcg cgctgatttt agtgtgactt tacgcgccat gcatctggct gcatgactat
     4081 gcatcagtgt aatatccagc ggctgcgttt ctggtgtttt ttcgtcatgt ctgtcgccac
     4141 tttctcgcgt ctggcaccgg aaacgcagcc aaccccgttt cagcggtgcg ggcaatggga
     4201 cgttatggta aataagtggg cgtaaaaaag cccgggttac gggcttctgg acgctatgac
     4261 ttaccacgac attctctgca ccaactttga ttcgttgcac cagaaggaat ggttctgaat
     4321 ccgaatagag tttgcaactc acctgcagta tgagcggtgg ttcgcttgca gttagggcat
     4381 gtatgaggaa gattcaccgt gcctaccatt tttctatcct taaacg
//
