LOCUS       NZ_CP056380             1916 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00636 plasmid pRHBSTW-00636_9,
            complete sequence.
ACCESSION   NZ_CP056380
VERSION     NZ_CP056380.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148670
            Assembly: GCF_013734035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00636
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2714513388374x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734035.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 03:00:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 65 of 181
            Pseudo Genes (incomplete)         :: 122 of 181
            Pseudo Genes (internal stop)      :: 19 of 181
            Pseudo Genes (multiple problems)  :: 23 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00636"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00636_9"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            982..1284
                     /locus_tag="HV253_RS28800"
                     /old_locus_tag="HV253_28805"
     CDS             982..1284
                     /locus_tag="HV253_RS28800"
                     /old_locus_tag="HV253_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1543..1779)
                     /locus_tag="HV253_RS28805"
                     /old_locus_tag="HV253_28810"
     CDS             complement(1543..1779)
                     /locus_tag="HV253_RS28805"
                     /old_locus_tag="HV253_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(29..202)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          181..672
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ccgcaggcgc gtcattagct tttagcgggt ttcggggcgc agccctgaac cagtcacgta
       61 gcgctagcgg agtgtatact ggctgactat gttggcacgg atgtggaaat cagtgaagtg
      121 cttcatgtag caggagaaaa aaggcggcac cagcgcgtca gcaggatatg tagtacagga
      181 tatattccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      241 tgccggctta caggcggggc ggaaatttcc tgggagatac caggaaggag cttaacaggg
      301 aagagataag gccgcggcga agccgttttt ccataggctc cgcccccctg acaaacatca
      361 tgaaatctga cgctcaaatc agtggtggcg aaacccgatg cggcttaaag ataccaggcg
      421 tttccccctg gtggctccct cgtgctcccc cgcattcctg cctttcggtt taccggtgtc
      481 attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt ccgcgaaggc
      541 agttcgctcc tggatggact gtatgcacga accccccgtt cagtccgact gctgcgcctt
      601 atccggtaac tatcatcttg agtccaaccc ggaaagacac gacttatcgc cactggcagc
      661 agccactggt aactgattta gaggagttag tcttgaagtt atgcgcctgt taaggctaaa
      721 ctgaaagaac agatttggtt actgcgctcc tcgtaagcca gttaccacgg ttcaaagagt
      781 tgctgctctg agaaccttcg aaaaaccgcc ttgcagggcg gttttttcgt ttacagagca
      841 agagattacg acgccacaga aaggatctca agaagatcct tcaccgtaat ggaaatctat
      901 ccaatccgag cacataggat acacatcgtt ataaaacatc ggatattatg atcccaatta
      961 aaataagctc gaggataaaa aatgagttca atggtttact gccgaggatg tggcaaggaa
     1021 atacacgaaa cagcaaagtc atgcccgcac tgcggagcca caaacgcctc ttcaggttca
     1081 ggtgaaaaaa gtagaatcgc agccgcgcta ttagcattct tccttggtgg atttggcgca
     1141 cataaatttt acttaggaaa aataggacag gggttcctgt atctaatttt ttgctggaca
     1201 ttcatacccg cgatcatcgc gtttattgag tttattattt atctatgcga ttccgatgaa
     1261 aaattcgcac gaaaatatgg ctaactcata gcaaaaaaaa gccccctaag ggcttttttt
     1321 gtacctgacg gtcagaccga ttaaacataa catcccttgc ccgcaccccc gcaggcgccc
     1381 tcagcgcaat cctgctgcat cagggccgtt tccagcacct ttcctcacca gtatcccgga
     1441 aaaacgcccc tgatgatttc tgcgcgactg catggctatg cacgcaggtg aatggcaggt
     1501 gatttcgcag cgtcagcacc actaaaccgc caaaagccaa aatcagcggc gcggcgcgcg
     1561 cggacgtgac caggcaaaat cggactgctg ccgctcatcc tcacgtaatg cacgctggcg
     1621 gtcggcgtaa cgctgcaact caatttgcgc acgcgctctg ttcaggacga tgtgccggtt
     1681 aggctgattt agctttttac ctgacgctct ttcagctgcg gcaacagcat catcaagaaa
     1741 ttttttctct gcaggcgtca agtttgtcag tctggccata acgttctcca gtcagaataa
     1801 atcagcacag tctgccagac acagtagaaa cgatgaagcg ccagcgacgg tgaaaggcag
     1861 tcagaaacgg tgaacgggag cggattttaa tccggtgccg tgaggcgttg gacaag
//
