LOCUS       NZ_CP056379             4018 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00636 plasmid pRHBSTW-00636_8,
            complete sequence.
ACCESSION   NZ_CP056379
VERSION     NZ_CP056379.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148670
            Assembly: GCF_013734035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00636
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2714513388374x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734035.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 03:00:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 65 of 181
            Pseudo Genes (incomplete)         :: 122 of 181
            Pseudo Genes (internal stop)      :: 19 of 181
            Pseudo Genes (multiple problems)  :: 23 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056379.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00636"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00636_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3720..4018,1..25))
                     /locus_tag="HV253_RS28770"
                     /old_locus_tag="HV253_28775"
     CDS             complement(join(3720..4018,1..25))
                     /locus_tag="HV253_RS28770"
                     /old_locus_tag="HV253_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            673..786
                     /locus_tag="HV253_RS29225"
     CDS             673..786
                     /locus_tag="HV253_RS29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1215..1427)
                     /locus_tag="HV253_RS28775"
                     /old_locus_tag="HV253_28780"
     CDS             complement(1215..1427)
                     /locus_tag="HV253_RS28775"
                     /old_locus_tag="HV253_28780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(1682..2167)
                     /locus_tag="HV253_RS28780"
                     /old_locus_tag="HV253_28785"
     CDS             complement(1682..2167)
                     /locus_tag="HV253_RS28780"
                     /old_locus_tag="HV253_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208484249.1"
                     /translation="MSFTVAAAENNDNVTYTKPSAGRDLYLTCKGYQVKGASVINVEKP
                     SYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMHEHWSLVHVGGRASV
                     KGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIYTRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(2338..2553)
                     /locus_tag="HV253_RS28785"
                     /old_locus_tag="HV253_28790"
     CDS             complement(2338..2553)
                     /locus_tag="HV253_RS28785"
                     /old_locus_tag="HV253_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2557..3042)
                     /locus_tag="HV253_RS28790"
                     /old_locus_tag="HV253_28795"
     CDS             complement(2557..3042)
                     /locus_tag="HV253_RS28790"
                     /old_locus_tag="HV253_28795"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3077..3781)
                     /locus_tag="HV253_RS29230"
                     /old_locus_tag="HV253_28800"
                     /pseudo=""
     CDS             complement(<3077..3781)
                     /locus_tag="HV253_RS29230"
                     /old_locus_tag="HV253_28800"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(139..316)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          263..786
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.434"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2247..3730)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cttctgtcac ccacatagtg agcattttgt tgcgcttttc tgccaaacca tcctccggat
       61 aacaccggtt cgggcgggaa attttttcct gtccggagcg gtgttgggca agcccgcagg
      121 gcgcggcagt acttttcagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
      181 gcggagtgta tactggctta acatggtaaa tcggagtggg gattcaggaa agtgcttcat
      241 gtggcaggga gaaaatgctg ctgtggggcg taagtgcgaa atgcagatcc cggtgaaatg
      301 acgcctcctc gctcactcgg tcgctgcgct cctgccgtga gactgcggcg ggcgttgcca
      361 gcttacgaat ggctggataa agagactaag cggaaatcaa tcagaaaggg cggaaaaagg
      421 atgcggcgtt gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
      481 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
      541 gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
      601 tatggccgcg tttatctaat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
      661 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
      721 tatcgacttg agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccactggt
      781 aactgattta gaggagttag tcttgaagtt atgcgccggt taaggctaaa ctgaaaggac
      841 aagttttggt gactgtgctc ctccaagcca gttacctcgg ttcaaagagt tgctgctctg
      901 agaaccttcg aaaaaaccgc cttgctgggc ggttttttcg tttacagtgc taagagatta
      961 cgattctcca tgaatgatct caagaagatc cgtttaaaaa agcgggtttt atggggggat
     1021 aaagttcgtc tagcccagtc actttggatt acagctaggt ttcgtaagcc atgcaagaat
     1081 gcctgtaact aacaaaatag gccctgcaac gtacgtaaca tagactccaa catacgacac
     1141 tgtgaaacat atgagtacgt atatagacag tgttgtccaa aaaatgactg ttgttttaca
     1201 gtgaatccac tctttcattt ttcaataaac cccacaccgt ctggagatct tattatcgtt
     1261 ttgccaggat tagctttcgc ccaaaccaaa gcatcttgaa aagaagagaa tttttttttt
     1321 gaatttttag tttttcggcg atcttctcga aaatattcgc tgttattctc atcaattatc
     1381 cgatcaaaat cagcaccagc tccaaaggta cgctcaaaat cccccatttt ctgcccccat
     1441 taaatttaat aaaaaaacag atttaaaata tccaaaagac actcttattt accataacat
     1501 cccttgttcg caccgccgca gacagcctca gcaccgttct gcgccatgaa ccgcaaaaga
     1561 gcacctctta ccacgtaaaa acgccgataa atgccccccg taagttctta cggtatgcat
     1621 agttatgcac ggaaatgaac tgacactctg ttcaatggat tttcatgctg taagcagtat
     1681 tttagtcttc tatgccccct cgaaacacac ctgttcttgt gtagatcttt tcgacccaag
     1741 atggtctttt atccgagttc atgcagccgt acaggaacac acctgttttg ggtttatagc
     1801 tccccccctc ctgtggctgg ttatctacaa atgaataatc aatcttttca cctttgactg
     1861 aagcacgccc cccaacatga actaaagacc agtgctcatg catcacatgg ctttcaatca
     1921 atccaaatga agaaaaaccc catgaatcaa aaaagacctt gagtccgtca gacgcaccaa
     1981 caggagtaat gattgcaaat tcaacctgct caccagtacc ttcaaggtaa gaaggtttct
     2041 cgacgttaat tacagatgcc cccttcacct gataaccttt acaggtaaga tataagtccc
     2101 tcccggctga cggttttgta tatgtaacgt tgtcgttatt ctccgcagca gcgactgtaa
     2161 atgacatagc caaaaccacc gagactgagc aagcccagaa acatttcata acgcatccct
     2221 tttcattgat taactacatt ccaagctcaa aacctttctg ccgctgcgat cgttccagct
     2281 ccagcgcttt ctggtgctgc gccgcgcgct cctgctcccg gtgcgcctcg atgccattca
     2341 gccttgcgac aatcggcgca gctgatcgct caaactctgc acctgcctgc tcaagcccgt
     2401 gacgcgctcg ctcagcgccg cgttctcccg ttgcataagc ccagacatcg tccgccattc
     2461 cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa
     2521 tgcgctcagc aactgttttt ccagttccgt catgtgctat ttccccgcca gtatcatcca
     2581 gctcgaatcc tctccgaatc ttcccgcttc cgggttcacc ctcacgcagc ggcgtctctc
     2641 ctccccgcag gtcgtgagct ggacgccgtt gttcttctct gaaagcatgg cctgcgtgct
     2701 cttctgttcc tggatggtcg tgtagttgtc gagtatctgt tgcccctgcc accacagaat
     2761 gcccgcgctc gaggcgatca gtagcgcgga gaccaggact atggtcagcc acgtctggct
     2821 gatcatacgc agcatccctt tcgtgcgttg gctcatggct gaggacagct tccggtcgtg
     2881 gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagactt
     2941 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta gtctgctgct gcgctttcga
     3001 tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc
     3061 ggatgtttcg ccccccgtca gggtcggcaa tgctgatgct gcttttcgtg gtgcgcacca
     3121 cctcaaaacc cgcactttcc agcgcctcag tgacgtcctg acgggttttc agctccccgg
     3181 atgaggcaag ggaaagtagg ccgcgcgtaa tcgcctgggc ggcttcctgc ttcgcttccg
     3241 gcagcgcgga cggtgtaacc agcgcccgcc ggttttccgg cgcgttcggg tcgtgcagcc
     3301 ccagtctgcc gttcactatg gtctgccagg catcaatgcg cggacggtcg gcgcggtcgt
     3361 aatacggctg gagacgtctg ccggtcagca gctccgtgtt cgggatcagg aagttcagct
     3421 ccagccgccc tttgtcccgg tgctcaaccc acagtacgct gtactggtct ttatcgagtc
     3481 cgggcatcag aacccgctca aagctcgcca tcagcttttc acgctggccg ggcggcaaat
     3541 cctgttcggc aaaagacagc acgccggagg tgtatttctt ggcgtaaggc gaggcgtcaa
     3601 taagctcccg cacttcctcc ggcttaccct gcagaacgct ggcgccgtcg cgctgccggt
     3661 ctttccccag cagataatcc accggaccgc cgccaccgcc gcggcctctg ggatgaaact
     3721 taacgatcat cgtctgcccc cttttccagc acggcgtgcc gcagccgctc gagtccggca
     3781 tcaatggcca tcagcacggc gacaacctgc acgcgatcgt gcccagtacc gccaccggcg
     3841 ttaacctgcc gggcaatctg gttgaggttg ttacccatgc cggcaagctg acgaagcagc
     3901 gccggcgaga tggatggaag tttgccagca cgcgagggct tttcgtccag gcatatctgg
     3961 cgcatccagg ccgcgagttg tttaccctca cagcgctcca gaagtcggcg gtgctcgt
//
