LOCUS       NZ_CP056378             4416 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00636 plasmid pRHBSTW-00636_7,
            complete sequence.
ACCESSION   NZ_CP056378
VERSION     NZ_CP056378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148670
            Assembly: GCF_013734035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00636
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2714513388374x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734035.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 03:00:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 65 of 181
            Pseudo Genes (incomplete)         :: 122 of 181
            Pseudo Genes (internal stop)      :: 19 of 181
            Pseudo Genes (multiple problems)  :: 23 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00636"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00636_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4154..4416,1..604))
                     /locus_tag="HV253_RS28740"
                     /old_locus_tag="HV253_28745"
     CDS             complement(join(4154..4416,1..604))
                     /locus_tag="HV253_RS28740"
                     /old_locus_tag="HV253_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063617447.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPLHSHWSPFWSGADLYELNDLADCFDDL
                     EDPKKRENTELAFGRNVEMFDTQRQWAYKNVLKYKNESSFDAFYKELLLKCETFNSFAN
                     YNNLLPYKELVSTAKSIAKFCWKEFSNEKLHDIQSKKQKARRSKRTEKQKLHTKTLLEK
                     AIMINEE"
                     /name="replication initiation protein"
     gene            905..1120
                     /locus_tag="HV253_RS28745"
                     /old_locus_tag="HV253_28750"
     CDS             905..1120
                     /locus_tag="HV253_RS28745"
                     /old_locus_tag="HV253_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001171587.1"
                     /translation="MQKPKRRLGFMVGKSNATLPPDDETEAELDQEVLKLFYPNQETQD
                     TIERSEAGTDVFMASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            1767..2027
                     /locus_tag="HV253_RS28750"
                     /old_locus_tag="HV253_28755"
     CDS             1767..2027
                     /locus_tag="HV253_RS28750"
                     /old_locus_tag="HV253_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063617446.1"
                     /translation="MNENDIDQSLLELLARYFYYIDPNEENTAFLEANKDEQDLCYFVA
                     YRYIKENKTQDLIDALKDQKDEDYIKAMKDYVYGGGKYGYR"
                     /name="hypothetical protein"
     gene            2014..2385
                     /gene="mobC"
                     /locus_tag="HV253_RS28755"
                     /old_locus_tag="HV253_28760"
     CDS             2014..2385
                     /gene="mobC"
                     /locus_tag="HV253_RS28755"
                     /old_locus_tag="HV253_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_063617445.1"
                     /translation="MDTDRKRGRIGLNHEVRRDISIQFRVNKAEFDYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQHQGNNINQIAKKLNTNEDFKQSLAAEAN
                     FAIKQLMKRILKACEVYTV"
                     /name="mobC"
     gene            2382..3866
                     /locus_tag="HV253_RS28760"
                     /old_locus_tag="HV253_28765"
     CDS             2382..3866
                     /locus_tag="HV253_RS28760"
                     /old_locus_tag="HV253_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_063923475.1"
                     /translation="MKGSVAKTGKSFKNRVDYILKDDHSFICSNMASDYNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAFLSLPKNEHLTDQQWEEIAKDYLKEMNIDIEKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITQSLKGQKSDVSAPT
                     KNEIEQALRTGEKPARIVLQNALQAAMVGKPDLQTFIDRVQAVGIEPRFNVASTGNVAG
                     VSFSVGDVAFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRSFSARKDDEPNSIRRSLAE
                     PNINDSGAVDRIDDDIIRISQHSIKSDTTDTYESKQLVRSNNHSFKLSEKYNRPASHKV
                     RRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSSSISRILDHAETANAATNN
                     FRLKGRGNINSRATKIEEIRERLGIKKPIIQPKNKEKIVKEEIKKKPLGWSMNENRFKS
                     KLKEALKKEKPKNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3875..4150)
                     /locus_tag="HV253_RS28765"
                     /old_locus_tag="HV253_28770"
     CDS             complement(3875..4150)
                     /locus_tag="HV253_RS28765"
                     /old_locus_tag="HV253_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_000125721.1"
                     /translation="MTALHTVKRNKTAKEMAQRLNISDRTVRKLIALPREDYEKEAKQR
                     RLKAFVLREAGFKWSEVGERLGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="HTH domain-containing protein"
ORIGIN
        1 agctttcatt cttatatttt agcacatttt tataagccca ttgcctttga gtatcgaaca
       61 tctcaacatt acgaccaaaa gcaagttctg tattttctcg ctttttaggg tcttctaaat
      121 catcaaaaca atcagctaaa tcattaagtt cgtataaatc agcaccagac cagaacggcg
      181 accaatggct atgcagtggg tttttagtaa tcagtcccgc atagccaaga tcagcaccaa
      241 gacgagtagc catattgtaa tataatgctc tggcaaactt gacaggcttc tgtctgcttt
      301 tctctgttga tgagatcgga gcattaagca tataaccgta atgacatcta ccagagtctg
      361 gtgattttgt tataaaagac gggatgggca aatgagcatt ttcaaactca taaaagcagt
      421 tttctttgtc taaatcaaat atcattgtat gaagatacaa cgggtgattt acttgtaaat
      481 aaagcatatc taaagcttta ttcttatttt gaatagtcaa tccaaaatca agatcattag
      541 tagcatatgg ctttaaaggt atttgacttt ttaatatctc tctgttattt tctatattga
      601 acatttagca gtatcctttc tgtaatgccc cgttctctca ctgtcgccaa actaatcaga
      661 gaacgcgggc ttttctttta tttataatct cttacagttt tactatacca aaaaaacttg
      721 tcaatagatc cgtcaaaaaa agttgttaag taggaagtct accaatatca ggtattagac
      781 ggagttttgg ggacactaca ccacagcgaa gcacaggccg aaggctatgg cagagtaagt
      841 cacactgtaa cgtatactaa acgtcatatg caggatctgg ctgtgctgga taggagaaca
      901 tgagatgcag aaaccaaagc gtagactcgg ttttatggtt ggtaagagca atgcaacatt
      961 gccaccagat gacgaaacag aagcagagct tgatcaggaa gtcttgaagc tgttttatcc
     1021 taaccaagag acacaagaca ctatagaacg ctcagaagcc ggtacagacg ttttcatggc
     1081 ctccggttct gatgacctgt tcaaaaaact gaagctgtag gtgaagctgt agggcagggg
     1141 agagatggcc atcttcacca cttgagaaca aaccgacccg gaacggtgag agaaggccaa
     1201 ccctcgatgc agtatatact aatatatact atagtctgtt gattttatga gcattttaaa
     1261 cccatagacg ccagcaacag cagaaaagca agttttctac tgttctgtcg ccgccgatca
     1321 accccatccc tttgcatctg gtgaaatctg actaccaaga ggtcacgaac tttgtcagat
     1381 ttcacgtagc agcagcattt tttacgcaaa gctacctatt acgcacagct acccatcaag
     1441 acaatatatt atttacccta ttttttattt gcattttatt tgttgtgcag ggtatttatt
     1501 tatgtagccg ctatattgac aaacttttta gatcggttat ttatttaaat ataggttgag
     1561 ttcagcttta tttttgtttt tcggaaactg aggcaaggtg taatcccgta gccggtatta
     1621 tacccaatcc ttcggattgc cctatctcgc tttgctcgta gggttgaaaa aaagatctac
     1681 ccttccgcct attcccctta tcaaagggga ggcggtaaac catagtttta cattgaatgt
     1741 tataaatata tataggagtg tttaaaatga atgagaatga tatagatcag tccttactag
     1801 aactattagc aagatatttt tattatatag acccaaatga agaaaataca gcatttcttg
     1861 aagcaaataa agatgaacaa gatttatgtt attttgtagc ttatcgctat attaaagaaa
     1921 ataaaactca agatcttatt gatgctttga aagatcaaaa agatgaagat tatatcaaag
     1981 caatgaaaga ttatgtttat ggaggtggta aatatggata cagatagaaa aagagggcgt
     2041 ataggattaa atcacgaagt acgtagagat atatctatac agtttagagt aaacaaagca
     2101 gagtttgatt atttaaaaga gcttgtggaa gcatcagggc ttgaaagagg tcattacatc
     2161 cgaaaggaga ttttaaactc tgaacccgtt ataaaaagga aaatgcctga gtttgagcaa
     2221 tcattataca gagaaataca acatcaaggt aataatataa atcagattgc taaaaagcta
     2281 aatacgaatg aagactttaa acaatcttta gccgcagaag ctaactttgc cataaagcaa
     2341 cttatgaaaa gaatattaaa agcttgcgag gtttatacag tatgaaagga agcgtagcta
     2401 agacgggtaa gtcttttaaa aatagagttg attatatatt aaaggatgat cacagtttta
     2461 tttgtagcaa tatggcaagt gattataata atgttagtga tttaactgat gagtttaaaa
     2521 ctgtaagcag ctttagacaa gatataaaaa aaccagtctt tcatgcgttt ttatctcttc
     2581 caaaaaatga acatttaacc gatcagcaat gggaagagat agcaaaagac tatttgaaag
     2641 aaatgaatat tgatatagaa aagcatcaat atatttgtgt tcgtcataac gatacagata
     2701 aagaacatat ccatattgta gctaatcgtg ttggccttga tggttctgta tggctcggtc
     2761 agcactcagc atttaacaca atagcagctt gtgaacgttt agaagtaaaa cacggcttaa
     2821 ctattacaca aagtcttaaa ggacaaaaat cagacgtatc agcaccaacc aagaacgaga
     2881 tcgagcaagc cttgaggaca ggtgagaaac cagcccgtat cgtgctacag aacgcattgc
     2941 aggctgctat ggtaggaaaa ccagatctgc agacgtttat agacagagtg caggccgtag
     3001 gcattgaacc gaggttcaat gttgccagca ctggcaacgt ggcgggggtt tcattcagtg
     3061 ttggagatgt ggcattcaag ggatctcagt tgggtaaaaa atacagttgg aacactataa
     3121 aaaacaaggt gatatatgac aaaaatagag atgatgaact cgttaggagc ttctcagcaa
     3181 gaaaagatga tgaaccaaat agtattagac gatcacttgc tgaaccaaat atcaatgaca
     3241 gtggagccgt tgacagaatc gatgatgata ttatccgaat cagtcagcac agcattaaat
     3301 cagacacaac agacacttac gagagtaaac aactcgttag aagcaacaac cacagcttta
     3361 aactcagcga aaaatataat agaccagcaa gccataaagt tagaagaaca acacaaaaag
     3421 ctgcacaaat actcaacaga gttaaaaaac aatacaataa agaaccaatc aaaacttata
     3481 ataaaaatgg cctcaatagt tggcgtgata aatctgatag cagttctatc agtaggatac
     3541 ttgatcatgc agaaacagcc aacgcagcca cgaataactt tagactcaaa ggccgtggca
     3601 acatcaatag tagagcaact aagatcgaag agataagaga aaggttaggt ataaaaaaac
     3661 ctattattca gccaaagaat aaagaaaaaa tagttaaaga agaaataaag aaaaagcctt
     3721 taggttggag tatgaatgaa aatagattta aatcgaagtt aaaagaagct cttaaaaaag
     3781 aaaaaccaaa aaatgaaaca acaccaacac aactacagca ggaagacgat cagcatttaa
     3841 aaccacgcgg ttttaaacca agataaaaga ggctttaagc ctcttttgtt gttggtgcgt
     3901 ctatttgttg ataacgttta taaagagcta tagccccgtg ttttgtagtt cccagccttt
     3961 ccccaacctc tgaccattta aagccagctt cacgtaatac aaaggctttt aatcttcttt
     4021 gttttgcttc tttctcatag tcttcacgag gtaaagcaat caactttctg acggttctat
     4081 cactgatatt taatctttga gccatttctt ttgctgtttt attccttttt actgtatgta
     4141 atgccgtcat attttactcc tcgttaatca ttattgcttt ttctaaaagt gtttttgtat
     4201 gtagcttttg tttttctgtt cttttagatc ttcttgcttt ctgtttttta ctctgaatat
     4261 catgaagttt ttcgttgcta aactctttcc aacaaaactt agctatactt tttgccgttg
     4321 atacaagttc tttgtaaggt aatagattat tatagttagc aaagctattg aatgtttcgc
     4381 atttcaataa taactcttta taaaaagcat cgaatg
//
