LOCUS       NZ_CP056377             5525 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00636 plasmid pRHBSTW-00636_6,
            complete sequence.
ACCESSION   NZ_CP056377
VERSION     NZ_CP056377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148670
            Assembly: GCF_013734035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00636
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2714513388374x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013734035.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 03:00:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,797
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 65 of 181
            Pseudo Genes (incomplete)         :: 122 of 181
            Pseudo Genes (internal stop)      :: 19 of 181
            Pseudo Genes (multiple problems)  :: 23 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00636"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00636_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(279..845)
                     /locus_tag="HV253_RS28705"
                     /old_locus_tag="HV253_28710"
     CDS             complement(279..845)
                     /locus_tag="HV253_RS28705"
                     /old_locus_tag="HV253_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043876049.1"
                     /translation="MAKYQIDSIQNWPSFEHDGSTYSLSHLDAHEITYKGDKQDFTFVV
                     TYGLHCFAKEDTPYVIPVVYQDGREEKTICMERYEASKHIRHILVNLPDMRLFHTASEK
                     YFTLDMMNSATGQLEPYKICIAFYKEKRLLRMHILSAFFVRYGPGFPGEPIPNKAVSLF
                     KVATDTASRQKKHAWIPKEANNRIK"
                     /name="hypothetical protein"
     gene            complement(1492..1791)
                     /locus_tag="HV253_RS28710"
                     /old_locus_tag="HV253_28715"
     CDS             complement(1492..1791)
                     /locus_tag="HV253_RS28710"
                     /old_locus_tag="HV253_28715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048269944.1"
                     /translation="MAFGNAYLVDDMTIQNFRIGFERIVFLGLLFFAVKAVLLFGIILF
                     HHGWNVWQRDRAFECLIIFAGIMISSQVLLWVAYGFAGKPFKPNFNPLTYVHRL"
                     /name="hypothetical protein"
     gene            complement(1760..2209)
                     /locus_tag="HV253_RS28715"
                     /old_locus_tag="HV253_28720"
     CDS             complement(1760..2209)
                     /locus_tag="HV253_RS28715"
                     /old_locus_tag="HV253_28720"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064159960.1"
                     /translation="MSKQGKNLLNLTQAAEAVGITRKTLYSHIDKGLVSVTRQQGKRYV
                     DVSELIRYYGSVSLPEEKVNTVTRSQTKHNDDTILAMQRELVELRKLIEKQQLLLEDKQ
                     EADRLREQVTGLQNERDAAAQELKKVKAELEAKRNNGFWKRLFGG"
                     /name="helix-turn-helix domain-containing protein"
     gene            2803..3309
                     /locus_tag="HV253_RS28720"
                     /old_locus_tag="HV253_28725"
     CDS             2803..3309
                     /locus_tag="HV253_RS28720"
                     /old_locus_tag="HV253_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048269941.1"
                     /translation="MTTKRQAQRATESDLQAFMNDAADRFKPDAAVLAARIDTAVQRYE
                     VTSTTQRFNAPAPLAMQQLQERILEGWRYDIGIPQSVYVAGTGNMSITLRKPASLVEQE
                     IADLKRQVEEGYHNELAAAMEREVDKLVQDAANEAQRRAEEAAAAERDAMRQRLRDMLL
                     SGATV"
                     /name="hypothetical protein"
     gene            3306..3449
                     /locus_tag="HV253_RS28725"
                     /old_locus_tag="HV253_28730"
     CDS             3306..3449
                     /locus_tag="HV253_RS28725"
                     /old_locus_tag="HV253_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170320547.1"
                     /translation="MTVTKKGPPCPAYIDPEALEKAIAAARKIREANPERYKPIPVQPK
                     LF"
                     /name="hypothetical protein"
     gene            3577..4239
                     /locus_tag="HV253_RS28730"
                     /old_locus_tag="HV253_28735"
     CDS             3577..4239
                     /locus_tag="HV253_RS28730"
                     /old_locus_tag="HV253_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008320537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="WP_048269942.1"
                     /translation="MNHAVTSHYDNGRYHLINSANYAFGDNTYSLNFKPDSKRYSPIRQ
                     EIMDSFITQLCAMQSHYSKIFAFRFDLSVPAGMPVAESNQLISELFTRLRGKFTAKRWN
                     GQPIKKFAYGWVREKEKAKQVHYHCWIALPHFQVRVAGFGNEGTYGLINTLWSELTAQK
                     GRSRPCNGTYTINRDDHSSLVALVERVSYLAKTRGKYSTGDGQRMFSASKLKILTAA"
                     /name="inovirus-type Gp2 protein"
     gene            complement(4476..5108)
                     /locus_tag="HV253_RS28735"
                     /old_locus_tag="HV253_28740"
     CDS             complement(4476..5108)
                     /locus_tag="HV253_RS28735"
                     /old_locus_tag="HV253_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689667.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_048269943.1"
                     /translation="MTSRVYAYLRASTKEQDASRAREQLDEFAHSKGMTIATYFTENES
                     GASLQRPELFRLLDIAQKDDVLLVEQVDRLSRLKADDWQVLRQTISSKGIRVVALDLPT
                     SHQLIQSGDEFTNRMLEALNGMMLDMLAAIARKDYTDRKRRQEQGISKAKEAGKYKGRP
                     EDAQRNEKIARLLNAGMSYTDIMGTVNCSRATVAKVSKSLKEAGITG"
                     /name="recombinase family protein"
ORIGIN
        1 tcgagaaaag ttagtatata ggtgcaacca aaatgttggt tgcaaatcta catcacacta
       61 cctacaattg ctttaaggat ggttacaggg atttcggtaa cgaaatcgca tcccacttcc
      121 tggtaacagg ttcgtccatg acggtaaagt gtcctgcccc gccttatggc ggctgagacc
      181 cccgaaaggg ggtcgccact ttctaagcct gaaaaaacac cctcagcttc aacttttcct
      241 gttggtcaaa gccagttcat caatgtacct gacgcatctc atttgatacg gttgttcgct
      301 tcctttggta tccaagcatg ttttttctgc ctgcttgccg tatcagtcgc gaccttaaac
      361 aaactgacag ccttatttgg gataggctca ccgggaaaac ctggaccgta acgaacgaaa
      421 aaggcagaaa gaatatgcat acgtagtaag cgcttctctt tgtagaacgc aatgcaaatt
      481 ttgtaaggtt caagctgccc cgtagcacta ttcatcatat ccagcgtgaa atacttctct
      541 gatgccgtat gaaacaatcg catatctggc agattaacca agatatgacg gatgtgcttg
      601 gacgcctcat agcgctccat acatatggtt ttttcttctc gcccgtcctg atatacaacc
      661 gggatgacat aaggcgtatc ttctttagcg aaacaatgta aaccataggt aaccacaaag
      721 gtgaaatcct gcttatcacc cttatacgtg atctcatgag catcgaggtg tgaaagagaa
      781 taagtagaac catcatgttc aaatgaaggc cagttctgaa tagaatcaat ctggtattta
      841 gccactgcat caatccatgt tggtcgaatc cgcatattct accagataag agacaagcgg
      901 aaggaaaccg gaaaaagaag ggccaggact tttacagaag gcttcgcccc tcctgacgag
      961 catcacaatt tagacgctca aatcaagagt ggcgaaaccc gacacagctt aaagatcccc
     1021 accgtttccg atgagtagct ccctcgtgcg ctcctgtgtt cccgccttga cgtttgcagc
     1081 cagccttgcc gctctttcta ccgtgggggc gctaactctc agcttcccta tgctggcact
     1141 gctcgtttta ggcggttcgc tccaagctgg atgatatgca cgaactcccc gtttcatccg
     1201 accgttgcgc ctgctgggta actattctca cgattccaac cccagcggac acggcaaaaa
     1261 gccgctaatg gcaacagcca atacggtaac gagtaatcag gggaaggtag gtcttgcaga
     1321 ttgcgataag cgcaagtaga cttgatatac agttttggtg acgtttgttc ccccgaacat
     1381 cgttacctgg ttcaaagagc tccaactcag agaaccttcg aaaagccacc ttgccgggtg
     1441 gttttttcgt ttcagggata atgcaccgac gcagccacag ggtcaaatta attaaagccg
     1501 atgcacatat gttaacggat taaagttagg cttaaacggt ttccctgcaa agccataagc
     1561 tacccaaagt agaacctgac tagagatcat tattccagcg aaaataatta agcactcaaa
     1621 cgccctatct cgctgccata cattccatcc atgatggaaa aggatgatgc caaagagtag
     1681 caccgcttta actgcaaaaa ataacaatcc cagaaaaacg atgcgttcaa agcctatgcg
     1741 aaaattttgt atagtcatat catccaccaa ataagcgttt ccaaaagcca ttattccgct
     1801 tagcttccag ctctgctttc acttttttca gttcctgagc cgctgcatca cgctcatttt
     1861 gcaacccggt cacctgttcc cgcagccggt cggcctcctg cttatcctca agcaataatt
     1921 gttgcttctc gatcagcttc ctcagctcga ccagctcccg ctgcattgcc aagattgtat
     1981 cgtcattatg tttagtttgt gaccgtgtaa cggtgtttac tttttcctct ggtaaggata
     2041 cagatccgta atacctgatt agctcggata catctacgta tcgtttacct tgctgacggg
     2101 taacactcac caaaccctta tcaatatggg agtaaagcgt tttccttgtg atacctaccg
     2161 cctcagcagc ctgtgtaaga ttgagtagat tcttaccctg tttactcacc gtatacccgc
     2221 cataaaatag atgtgtttac tctgcatagt atacaaaaaa acagccaatc tgtagatgcg
     2281 aaagtttctc atctgtgata aaataggaac tgaccaataa accacacaag acaggagaaa
     2341 tattgacctt aacgcccaca ctgccaacgt ttgcagcccc gaccgatacg gaacgcgcca
     2401 ccctcgccgc gatcctgaat gatactggcc tccgcgccac aaatccccgg attaacgtct
     2461 tacactacct gaacgcaacc gacactcctg taaccgctga gatgatcagc cgcttcgttg
     2521 acctcccgtt atcgactgcg tacagaacgc tcacggcgct ggaagtcacc cacctcgcag
     2581 gggtcacttt cggacgcggg gacgttactc gttggttcag gtttacaccg gatactcccc
     2641 agcattgccc ggcgtgcgga cagagttttt acggcgaata cgcctgacca tctgagacac
     2701 aaacaccgaa aagatgaccg catgaacggt cacaccaaat gacgcccaca gagagcgcca
     2761 gcggcctgtt acggccttta ttcaccgatt acggagacaa ctatgactac aaaaagacag
     2821 gctcagagag ccactgagag cgatttacag gcattcatga acgatgccgc cgaccggttc
     2881 aaaccagacg ccgccgtgct ggccgcgcgg atcgataccg ccgtacaacg ttacgaagta
     2941 acgagcacca cacagcgttt caacgctccg gcaccactgg cgatgcaaca gctacaggag
     3001 aggatattgg aggggtggcg gtacgatatc ggcatcccgc agtccgttta cgttgccggt
     3061 actggaaaca tgagcatcac cctccgtaaa ccggcgtcgc tggtggaaca ggagatcgcc
     3121 gacctgaaac gacaggtgga agagggctat cacaacgaac tcgccgccgc gatggagcgt
     3181 gaggtagaca aactggttca ggacgccgct aacgaggcac agcgccgagc agaagaagcc
     3241 gccgccgcag agcgtgatgc tatgcgccaa cgcctccgcg atatgctcct gtcaggggcc
     3301 actgtatgac cgtcaccaaa aaggggccac catgccccgc ttacatcgac ccggaggccc
     3361 tggaaaaggc gatcgccgcc gctcgcaaaa tccgggaagc caacccggag cgctacaaac
     3421 ccatccccgt ccagccgaaa cttttctgag taaaaacgct ggcaataatt gcatctccgc
     3481 gtcagcagtg gcgcggcata ggcagacttt gctaaagcag taaccgtact ggtttttact
     3541 aatacctgta cataccaacc atcaactggg ggaacattga atcatgcagt aaccagccat
     3601 tacgacaacg gcagatatca ccttatcaac tcagctaact acgccttcgg cgacaacacg
     3661 tatagcctga acttcaaacc tgacagcaag cgctactcgc caatccgtca ggagataatg
     3721 gacagcttta ttacccagct ctgcgcgatg cagtcccatt acagcaaaat cttcgctttc
     3781 cggttcgacc tgtcagtccc ggcaggaatg ccagtagctg agagcaatca gctgatcagt
     3841 gagctgttca ccagactacg cggcaagttc actgcaaagc gatggaatgg acagccaatc
     3901 aagaaattcg catatggctg ggtaagggaa aaagagaagg ccaagcaagt tcattaccat
     3961 tgctggatcg cattacctca cttccaggtc agagttgctg gattcggcaa tgagggaaca
     4021 tacggcctga tcaacactct ttggagtgag ctcacagccc aaaagggccg ctcacgcccg
     4081 tgtaatggca catacaccat caaccgggat gaccactcat cgctggtagc actggttgag
     4141 cgggtctcat accttgccaa gacgcgaggg aaatactcca ctggagacgg gcagcgtatg
     4201 ttttctgcat caaagttgaa aatactgact gctgcttaac cggcttcgat tccaccgggc
     4261 cagccatatg cacacgaaaa atatctgggg tctttcccat gacagatttg acgacagcag
     4321 gcacagtaac gcccacagcg atccacgcgc cgccgttttt ctcacatcaa atcaatctaa
     4381 atgattgtta tttaataaaa aaaatccccg cacatcgcga ggatctcaat acacccgacc
     4441 gtctcaggtc ggcactctgg attgtggaag gcgggttagc ccgtgatgcc ggcctctttc
     4501 agactcttgc tcactttggc aaccgtcgca cgggaacagt tcaccgttcc catgatgtcg
     4561 gtgtatgaca tgccagcatt cagaagtctg gcgatcttct cgttgcgctg ggcgtcttcc
     4621 gggcgaccct tgtatttgcc agcctccttc gccttgctga ttccttgctc ctgacgacgt
     4681 ttacggtcgg tgtagtcctt acgtgcaatg gcggcaagca tatccagcat catcccgttc
     4741 aatgcttcca gcatacggtt agtaaactca tcgccggact ggattagctg gtggctggtc
     4801 ggtaagtcta acgctacaac cctgatcccc ttgctgctga tggtctgccg caatacctgc
     4861 cagtcgtccg cctttaagcg gctcaggcgg tctacctgct ccaccagcaa tacgtcatcc
     4921 ttctgtgcga tatccagcaa ccggaacaac tcagggcgct gcaacgacgc tcccgactcg
     4981 ttctctgtga aataggtggc aatggtcata cccttagaat gagcgaactc atcaagctgc
     5041 tctctggcgc gtgatgcgtc ctgctcttta gtcgaggctc ggaggtaggc ataaacacgt
     5101 gatgtcatag cggtatccct tatcagtctg tttttagtgg ttcaataata acagtctatt
     5161 aagagtggtt caataaatgt ttttcccggc ctgactggtt caataaacca gtgcctcttt
     5221 aggtatacct aaaccagacc gcatgacagc cagcatatcg atcaacccac cggcagatat
     5281 tttgaaaaga tcggcggtaa atcgtgaagg gggagaacat ccgcctaaca cctcagccca
     5341 tgcgccagcc gtacaatcac cggcaggata tttgcagcca gccggtttac gtatggtggg
     5401 ggtatcagcg atctcgcgcc ggaggggggt tataaatatg caacacgtga tatcaggtta
     5461 ggctttaggt tctctttagt cggctatttc aagaaagcca gatacagcaa ggggttgcgg
     5521 ggaga
//
