LOCUS       NZ_CP056367             7720 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00658 plasmid pRHBSTW-00658_3,
            complete sequence.
ACCESSION   NZ_CP056367
VERSION     NZ_CP056367.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148674
            Assembly: GCF_013739375.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00658
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.849774211442x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013739375.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:03:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,967
            CDSs (total)                      :: 4,846
            Genes (coding)                    :: 4,751
            CDSs (with protein)               :: 4,751
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 25 of 95
            Pseudo Genes (incomplete)         :: 63 of 95
            Pseudo Genes (internal stop)      :: 22 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056367.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00658"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00658_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7268..7720,1..189))
                     /locus_tag="HV257_RS24610"
                     /old_locus_tag="HV257_24605"
     CDS             complement(join(7268..7720,1..189))
                     /locus_tag="HV257_RS24610"
                     /old_locus_tag="HV257_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019282081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="WP_044864689.1"
                     /translation="MTIPMQPKIYHIVHVDRLASILASNGLLCDARIIAQGAAGTTIGM
                     NSIKQRRLTELTLSSYPDLYVGQCVPFYFCPRSIMLYVIHRADSDELAYKGGQGPIVHL
                     QADLHSCVKWAQKNQRRWAFTLSNAGSYYFEDKNNLSDLNQLEWSAINATHWQRCKEGK
                     QAEFLMEDSFPWHLIEAIGVQSKAIYTQVVNTLKSAAHRPPVSINTHWYY"
                     /name="DUF4433 domain-containing protein"
     gene            complement(496..786)
                     /locus_tag="HV257_RS24615"
                     /old_locus_tag="HV257_24610"
     CDS             complement(496..786)
                     /locus_tag="HV257_RS24615"
                     /old_locus_tag="HV257_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_142431636.1"
                     /translation="MKPSLELEKHREAIRELVHKYGLHNPRIFGSVAKGTDTEVSDLDL
                     LVDAGKKTSLFDMGGLHEELSALLSCPVHVLTPNEIRTDIRVKVLFYAIPL"
                     /name="nucleotidyltransferase family protein"
     gene            2336..2659
                     /locus_tag="HV257_RS24620"
                     /old_locus_tag="HV257_24615"
     CDS             2336..2659
                     /locus_tag="HV257_RS24620"
                     /old_locus_tag="HV257_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181548355.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGAGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2868..>3302
                     /locus_tag="HV257_RS24965"
                     /pseudo=""
     CDS             2868..>3302
                     /locus_tag="HV257_RS24965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3337..3822
                     /locus_tag="HV257_RS24630"
                     /old_locus_tag="HV257_24625"
     CDS             3337..3822
                     /locus_tag="HV257_RS24630"
                     /old_locus_tag="HV257_24625"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142431637.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASGAGILWWQGNRIL
                     ENYTIIREQKSTQAMLSERNSGVQLSTCGDQRRRCVRVNPQAGGFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3826..4041
                     /locus_tag="HV257_RS24635"
                     /old_locus_tag="HV257_24630"
     CDS             3826..4041
                     /locus_tag="HV257_RS24635"
                     /old_locus_tag="HV257_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4397..4534
                     /locus_tag="HV257_RS24640"
                     /old_locus_tag="HV257_24635"
     CDS             4397..4534
                     /locus_tag="HV257_RS24640"
                     /old_locus_tag="HV257_24635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181548354.1"
                     /translation="MAMQSGEIYQAQAARFTGEFAAGFAGLTPHSRRTRPGRCGQGMLW
                     "
                     /name="hypothetical protein"
     gene            4852..5262
                     /locus_tag="HV257_RS24645"
                     /old_locus_tag="HV257_24640"
     CDS             4852..5262
                     /locus_tag="HV257_RS24645"
                     /old_locus_tag="HV257_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044864692.1"
                     /translation="MAFISLSNLAAKTGRVVQSQHVTATQTKKSTGAVELGFRLSKSAM
                     DETGIAIGDRVDILYDKDENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEDENKKLLSDDSITQLSEGVIIFRLGDE"
                     /name="hypothetical protein"
     gene            5520..6083
                     /locus_tag="HV257_RS24650"
                     /old_locus_tag="HV257_24645"
     CDS             5520..6083
                     /locus_tag="HV257_RS24650"
                     /old_locus_tag="HV257_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044864691.1"
                     /translation="MRALSIPPSVARASLASKFSSHLKVISLFNSMQDSQVVVLSSLIE
                     GHHLTSSGNNVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTVQTVYLIE
                     PEHIEQLFTDPELVFANQERTLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLIHHAYKEK
                     PLSEEMWLDIEEKFIHMLDELNAA"
                     /name="hypothetical protein"
     gene            complement(6214..7254)
                     /locus_tag="HV257_RS24655"
                     /old_locus_tag="HV257_24650"
     CDS             complement(6214..7254)
                     /locus_tag="HV257_RS24655"
                     /old_locus_tag="HV257_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_044864690.1"
                     /translation="MIEITRGDILRADAEAIVNTVNCVGVMGRGIALQFKKAWPDNFKA
                     YAIACKKNEVHPGKMFIFETGQLTNPRFIINFPTKRHWKGASKIEDIESGLASLVGDIQ
                     KMGIKSIAIPPLGAGLGGLEWPLVCEKIKHALQPLSDVHVFLYEPSGAPQNDKMAKQKE
                     TPKMTAGRAVLIELIQRYLKGLLDPSISLLEVHKLLYFMQEAGEPLRLKYQKAHYGPYA
                     QNLRHVLNALEGHFISGYADGGDFPEKELQLVPGAIEEARDFLEKHPSTRQRFERVSRL
                     VEGFESPQGLELLSTVHWVNKHEQVKNSKDVIEAIYSWNDRKRQLTARQISIAEKVLDR
                     QNWLTA"
                     /name="macro domain-containing protein"
     Typing          2048..2224
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.556"
                     /coverage="82.0"
                     /evalue="1.63e-44"
     Typing          complement(1583..2073)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.282"
                     /coverage="94.0"
                     /evalue="1.33e-138"
     Typing          2650..4145
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.978"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcaggatag ctactaagtg ttaactcggt gaggcgacgc tgtttaatgc tattcatacc
       61 aatcgtcgtt ccagcagcgc cttgagcaat gatacgcgca tcgcaaagca gcccatttga
      121 agcgaggatc gacgcaagtc ggtctacatg cactatgtga taaattttag gctgcatagg
      181 tatagtcatt tagcaccctt ctccatgatt tttattatat aagtttttgt tttgtttgtt
      241 aatttatacg tattgttagc tctctaaaac cttacctact atgaccagct catcatgata
      301 tgtgaggtta aaagcttcct tctttgcatg gtttggactt caaaccaact gctttgctgc
      361 tcattagccg caaccaggct gcatattatc atcgcatatg tttgtaaaat tgaccgtaat
      421 gcaggcgaaa aatggcctag attttccagt ctgaaaatta tcattttgaa ttaatgggat
      481 agcttctgct tagggtcaga gtggaatagc gtaaaaaagt accttcacac gtatatcagt
      541 cctgatttca ttaggagtta ggacatgaac cggacatgac aatagtgcgc ttaattcttc
      601 atgcaatcct cccatatcaa agagggaggt cttttttcct gcatcgacca gcaaatcgag
      661 atcactgact tctgtatctg tcccttttgc aacagagcca aaaatacggg ggttatgcag
      721 accatattta tgtacgagct ctctgatagc ttcgcggtgt ttttccagct caagtgaggg
      781 tttcatgacg attgtcctgt tatgtggcgg ctgaatactg tgttgatgat actatcggta
      841 aatcaattgc gtgagacaat agtgggacgg gtgatactta tggcttggca taaggtgaga
      901 caggcacaga aattgacggg gaaagctcgt tccagtctgt accgtgatat gtcatcaggc
      961 cgcttgtcat acaggactga ttcggacggt gggcgctcca ttgatacaag cgagttaatc
     1021 cgagtctatg gagagttgag acaagaaggg acagtgtcac gggacactga gagacatagt
     1081 gatgagacac acaatcaggt aatgagtgaa cttattcgtg aaataaaatc ccttcgtgat
     1141 gaagtttccg ggctaaggaa cgaattgcag gaggtgagac gcatagagga caagacggaa
     1201 gtcgctgaaa catcaaataa aaaacagtct tggtggcggc gtttaatctc atagtgtcct
     1261 gttactagac tctccagaga ctgttactat agttgtttga aggctgtttc tatacttaac
     1321 ttggcgtttt gaaaaggttt tttgctggag agttatcttg acgcgatccg tggttgcggc
     1381 gtaaattagc ccctgaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt
     1441 cagttgggga actgcttaac caggtaactg gcttcgagag agcgcaatta ccaaatccgt
     1501 ccttccagtg tagccgtagt aaggctacaa cttcaagaac tctgtaacca ctcggttata
     1561 aatttccgct ctctcaagcc cgttaccatt ggccgctgcc aatggcgatt agtcgtgtcc
     1621 atccgggttg ggatcatgtt tgttagttac cgggtgagac gcagtggtcg gactgaacgg
     1681 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     1741 gtggaatgag ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     1801 acaggagagc gcacgaggga gcgacccacc ggaaacggtg gggatcttta agtcctgtcg
     1861 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     1921 tatggaaaaa cggcaacgcc gcatcctttt tccagcctct cctgatttga gcgttacacg
     1981 cttaatcccg tcatccgtta gctggcaacg cccgccgcag tctcacggca agagcgtagc
     2041 gaccgagtga gcgaggaggc gtcatttcac cggcactggc attttgcact tacacttagc
     2101 acctgcattt ttcacctgcc acatgaagca cttccctgaa ttaccactcc gatctaacat
     2161 attaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac
     2221 ccgccaaaag ccactgacgc gcctgcggct tgtccaacac cgcgccgacc gggaaaatat
     2281 ttcccgcccg tcccggcgtt atcaggaggc cggcttggca gacaaacgca gcaaaatgct
     2341 cacgatgtgg gtgactgagg atgagcaccg gcggctgctg gaacgctgcg acggcaaaca
     2401 gctcgcggcc tggatgcggc agacgtgcct ggacgagaag ccagcccgca ccggcaaact
     2461 tccgtcgctc tcgccggcgc tgcttcgtca gcttgccggc atgggtaaca acctcaatca
     2521 gatcgccagg caggttaatg ccggtgcggg cagcgggcac gaccgcgtgc aggtggtcgc
     2581 cgcgctgatg gccatcgatg ccggactcga acggctgcgg cacgctgtac tggaaaaggg
     2641 ggcagacgat gatcgttaag tttcacccgc gtgggcgtgg cggcggcgcc ggtccggtgg
     2701 attatctgct cggtaaagac cggcagcgtg acggcgccac tgtcctgcag gggaagccgg
     2761 aagaggtgcg ggagcttatc gacgcctcgc cctacgccaa aaaatacacc tccggcgtgc
     2821 tgtcctttgc cgaagaggac ttaccgcccg gccagcgtga aaagctgatg gcgagcttcg
     2881 agcgggttct gatgcccgga ctcgataaag accagtacag cgtgctgtgg gttgagcacc
     2941 aggacaaggg gcggctggag ctgaacttcc tgatcccgaa cacggagctg ctgaccggca
     3001 aacgtctcca gccgtattac gaccgcgctg accgtccgcg catcgatgcc tggcagacca
     3061 tcgtgaacgg caggctggga ctgcacgacc cgaacgcgcc ggaaaaccgg cgggcgctgg
     3121 tcacgccgtc cgcgctgccg gaagcgaagc aggaggccgc tcaggcgatt acgcggggtt
     3181 tactgtcctt agtctcgttc ggggagctga aaacccgtca ggacgtcact gaggcgctgg
     3241 aaaatgcggg ttttgaggtg gtgcgcacca cgaaaagcag catcagcatt gccgacccgg
     3301 acggggggcg aaacatccga ctgaagggag ccatctatga acagtctttt aacgctggcg
     3361 aaggacttag agcagaaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc
     3421 gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcga gaagagaatc
     3481 agcgccgcca tcctcgacca cgacaggaag ctgtcctcag ccatgagcca gcgcacgaaa
     3541 gggatggttc gcatggtcag ccagacgtgg ctgaccatcg tcctggtctc cgtgctgctg
     3601 atcgcctcgg gcgcgggcat tctgtggtgg caggggaacc ggatactcga gaactacacg
     3661 atcatccggg agcagaagag cacgcaggcg atgctgtcag agcggaacag cggcgttcag
     3721 ctctcaacct gcggagacca gagacgccga tgcgtgaggg tgaatccgca agcgggcgga
     3781 ttcggagagg actcgagctg gatgatactg gcggggaaat agcacatgac ggagctggaa
     3841 aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag gctggacgag
     3901 tgggagaacg ccttcgcgga atggcggacg atgtctggtc ttatgcaacg ggagaacgcg
     3961 gcgctgagcg agcgcgtcac gggattgagc aggcaggtgc agagtttgag cgatcagctg
     4021 cgccggttgt cgcaaggctg aacggcatcg aggcacaccg ggagcaggcg cgggcggcac
     4081 agcaccagaa agcgctggag ctccagcggg cacagcgaca gaaaacctat aacgggccaa
     4141 cgctgggctg attcagcctg cctgacacct gacaaacccc aagaataagg gcttcaaaaa
     4201 ttcccgctaa atacgtcgtg atgaaaatat cacggctcaa aagaggggga tttatgaaga
     4261 tactaaaagc gctcaggctg atactgcaat gcgccaaact ggcaatggat ctgtgggagc
     4321 gatatggggg aaagtggcct ttttagtgct ttaccccgta tttatacgcg aaaactgctg
     4381 tcattcacct ggctgcatgg ctatgcagtc cggtgaaatt tatcaggcgc aggcggcgcg
     4441 gtttaccggg gagtttgctg ccggttttgc cgggctgacg ccgcattcgc ggcgaacccg
     4501 tccggggcgg tgcgggcaag ggatgttatg gtaaatggga gcagatctgc atgtataaaa
     4561 aagccgtcta tatagacggc ttttttatgc ctatctgatg atttagctat atgcgctcac
     4621 gctgttctta tttaaatctg aagcaaaaaa tagcctttaa ttacataaag atcgatctgg
     4681 cagcatagct tgacgagatc tactacgaga agttatttga aattttacgg gttgaaattg
     4741 tggttgcatg gggtctcgtt agaggtagta caaaaagatc ttgaagtccg cagcgatccc
     4801 tggttacact gttgtgcaag aactgatcga tgtttaaaac gggttgcact aatggctttt
     4861 atctctttat caaatttggc agcgaagacc ggaagagtgg tccagtcgca gcatgttaca
     4921 gccacgcaaa cgaaaaaaag cacaggcgct gtggagctag gcttccgctt gagtaaaagc
     4981 gctatggacg agacaggaat tgctattggg gatcgtgtcg acattcttta cgacaaagac
     5041 gaaaaccaat ggatgattaa gaaaattagt gagggtggct ttggtgtttc tgggcaaaaa
     5101 aacaagcatg gtgggtactc atcatgtgct gttagattga ccttacgtcc tggttttccg
     5161 cgtctttcgg aggatgagga tgagaacaaa aagcttcttt cagatgattc aatcactcag
     5221 ctaagtgaag gtgtaatcat tttcaggctg ggtgatgaat aggaaagacc aaaaaggaaa
     5281 ggcagagtta gtgaacgtcg ccaaacaatc gactaactcc gccttctggt caacacatca
     5341 ctacctttgg aagggtggag tgttatgggc ggcattatgt tcataaactt gcattctaat
     5401 aaagaagaaa tcatttcttt tgatgagtgt gaacacgaca ttttctattc tgttaggtgc
     5461 ggtgctattg actttggtct cttaacttta tgtttttaaa catatatgag gctacacata
     5521 tgcgcgcttt atcaatacct ccctcagttg cccgagccag cctagcgagt aaattttcgt
     5581 ctcatttgaa agttatctcg ctcttcaact cgatgcagga ttcccaagtg gtggtcctgt
     5641 catctctgat cgaaggccat catctcacca gttctggtaa caatgttaaa gctgactttg
     5701 aagtcactcg cttaccagca atcatcgaga tgctagagaa aaaatatttt ttccctattc
     5761 gtcatctaaa tgtctctgtt aaatcagtaa ctacgggaag gatgactgtt caaactgtct
     5821 atctcataga gcctgagcat atcgagcaac tgttcactga tcctgaactt gtgtttgcta
     5881 atcaggaaag gactcttttc tttcgttctc tagaaaaaga gggaaaaaac cttggcaagc
     5941 ttatcgagaa aaagggcagt gtgtctcaag ctgttttaag cctcatacat cacgcttaca
     6001 aagagaaacc tctttcagaa gaaatgtggc tagatatcga agagaagttc attcatatgc
     6061 tggatgaatt gaacgcagcg taaaaaaggg acgttacatt caaccctgca gtatagcggg
     6121 ttcagatgtg aaaaggatcg ctcctggtga gcgatccttt ttttgtaggc gaaaagagat
     6181 tttagtaaat agcacctaag tttttgaaaa tagttaggca gttaaccagt tttgcctgtc
     6241 gagaaccttt tcggctatag atatttgtct agctgttagc tgccttttcc tatcattcca
     6301 agaataaatg gcttcaataa cgtctttaga gtttttcact tgttcatgtt tatttaccca
     6361 atgaactgtt gataaaagct ctagtccctg tggggactca aatccttcaa caagacgcga
     6421 tactctctcg aatctctgac gtgtacttgg atgcttctcc aaaaaatcgc gagcttcttc
     6481 aatagctcca ggaacaagct gtaattcttt ttccgggaag tcaccgccat cggcataccc
     6541 agaaatgaag tgaccctcaa gagcgtttag tacatgacgc aagttttgcg cataagggcc
     6601 atagtgcgct ttctggtatt ttaagcgtaa tggctcccct gcttcctgca taaaatacaa
     6661 tagcttatgt acttcaagta gagatattga gggatcaagc aatcccttga ggtagcgctg
     6721 tataagctct attaataccg ctctgcctgc ggtcattttt ggtgtttctt tttgttttgc
     6781 cattttgtca ttttgaggag caccagacgg ctcatataga aaaacatgaa cgtcacttaa
     6841 tggctgtaat gcgtgtttga ttttttcaca aactaggggc cactcgagcc cccctaatcc
     6901 agcacccagc ggtggaatgg ctatcgactt aatacccatt ttttgtatat ccccaactaa
     6961 ggaggctaaa ccagactcaa tatcttctat tttgctagcg cctttccaat gccgtttagt
     7021 tggaaagttt attatgaaac gagggttcgt aagttgccct gtttcaaaaa taaacatctt
     7081 cccaggatgc acttcgtttt tcttgcatgc aattgcgtaa gctttgaaat tgtcaggcca
     7141 agctttttta aattgcaatg caattcccct acccataact cctacgcaat tgactgtatt
     7201 tacaatagct tctgcatcag ctcgcaaaat gtcaccacgg gttatctcga tcacttttac
     7261 ggcctcctca gtaataccaa tgagtgttaa tactaactgg tggtctatgt gctgctgatt
     7321 tcaatgtgtt aaccacctgc gtatagattg cttttgattg aacaccaatt gcctcaatca
     7381 aatgccaagg aaaactatct tccatgagaa attcggcttg cttcccttct ttacatcttt
     7441 gccagtgagt cgcgttaatc gcactccact ctagttgatt taaatctgac aaattatttt
     7501 tatcttcaaa gtagtaagag ccagcattgg ataaagtaaa tgcccagcgg cgttgatttt
     7561 tctgcgccca cttaacgcaa gagtgaagat cggcttggag atgaactatt ggaccttgtc
     7621 ctcctttgta agctagctca tcactatctg cacgatggat tacgtacaac atgattgatc
     7681 gtgggcaaaa gtagaagggc acacattgac ctacatataa
//
