LOCUS       NZ_CP056364             3724 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00667 plasmid pRHBSTW-00667_4, complete
            sequence.
ACCESSION   NZ_CP056364
VERSION     NZ_CP056364.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148675
            Assembly: GCF_013746155.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00667
  ORGANISM  Citrobacter sp. RHBSTW-00667
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00667
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.4144395815584x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,260
            CDSs (total)                      :: 5,142
            Genes (coding)                    :: 4,959
            CDSs (with protein)               :: 4,959
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 47 of 183
            Pseudo Genes (incomplete)         :: 139 of 183
            Pseudo Genes (internal stop)      :: 35 of 183
            Pseudo Genes (multiple problems)  :: 32 of 183
            Pseudo Genes (short protein)      :: 1 of 183
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056364.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Citrobacter sp. RHBSTW-00667"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00667"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742659"
                     /plasmid="pRHBSTW-00667_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3250..3724,1..647)
                     /locus_tag="HV258_RS26095"
                     /old_locus_tag="HV258_26105"
     CDS             join(3250..3724,1..647)
                     /locus_tag="HV258_RS26095"
                     /old_locus_tag="HV258_26105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181553786.1"
                     /translation="MEFKIKKMTVKDSREIKKKFEDCIKIAEEKNMQVGSILNIEMQDR
                     LKEINLKVKKETAEQYRNLFNYKLSVNAEDIIQHLLECNTRNSFDKTRSAFRFCIAEKI
                     QELRNEADRARKQKNFDLMKEKTIEAYKLFFFFERDFLSENRVTWNDISYRKIDSKSKK
                     KTMNSVSSIKAIFEDLKQKPELFEKYSMILAISSLTGCRPAEIQKGIGLELSPPLLNIC
                     ISGAKVGEDRGQEKRILKFNINEFAENEQFKLIMSKIKQNNKLEYKCSKQEYDSLRQYL
                     YINHPGFSLYTLRHRAASELKKDGFSEEMIAAFLGHRVTRSLENYGYAKSGKGGPKIAG
                     VEHTNAIKSNTKRYKSKTTPGGGNPSPTRKRAL"
                     /name="hypothetical protein"
     gene            1861..2109
                     /locus_tag="HV258_RS26100"
                     /old_locus_tag="HV258_26110"
     CDS             1861..2109
                     /locus_tag="HV258_RS26100"
                     /old_locus_tag="HV258_26110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063145815.1"
                     /translation="MEIEKLIDRYFLALERKVIREIYDKQTREELEKFIFKCNGEFREF
                     ERRFNTICSTEYYEQQSLIFYINYINAEFEQREKLNY"
                     /name="hypothetical protein"
     gene            2175..2876
                     /locus_tag="HV258_RS26105"
                     /old_locus_tag="HV258_26115"
     CDS             2175..2876
                     /locus_tag="HV258_RS26105"
                     /old_locus_tag="HV258_26115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063861804.1"
                     /translation="MTISKKNSELRERFKEYSSNKNIFDLADLRYEILKIYFDFKLKND
                     MNEQERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELTKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNFETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     PKIQPIKKRRL"
                     /name="hypothetical protein"
ORIGIN
        1 ctaaaagcaa aaaaaagact atgaatagtg tttccagtat taaagcaatt tttgaagatt
       61 tgaagcaaaa gccggagctt ttcgaaaaat attcaatgat actggctatt tcaagtttga
      121 ccggttgccg tccggctgag atccaaaaag gaattgggct ggaactaagc ccaccactat
      181 taaatatatg tattagcggg gcgaaagtgg gcgaagatcg aggacaggaa aaaagaatat
      241 tgaaattcaa tataaatgaa tttgctgaaa atgagcagtt caaattgatt atgtcaaaaa
      301 taaagcaaaa taataaatta gaatataaat gctcaaagca agagtatgat tctttgagac
      361 aatatttata tattaatcac ccaggttttt ctttgtatac attaagacat agggcagcaa
      421 gtgaattaaa aaaagacgga ttttcggaag aaatgatcgc tgctttcctg gggcacaggg
      481 taaccagatc tctagagaac tatggatacg caaaatctgg gaaaggtggt ccaaaaatcg
      541 caggtgttga gcacacaaac gcgattaaaa gcaatacaaa gcgatataag agcaaaacca
      601 cccctggagg gggaaaccct tccccaactc gtaaacgagc cttatagtcg attttagagc
      661 gttacagtgg gtcagcaatg ctggcccttt atatgtgtgt tagcctcgcg cgcgcgtctt
      721 gccggcacag atttgtggtg actggttgca tatgtgtatt agcagaaccg caggttggtg
      781 tggttgcttg tgcggtcggt gtaattaact aatcaatata tgtattagca ggcggcacgc
      841 gtgcgtaatg cccggaacgg gaccccggta acttttcgtt ggtggttttt tgagcaatta
      901 actgtcagtc gttgacattt gaagtgtcat ttttgctgtt aaaaatgaca caacgagtct
      961 cattgagtga gaattttgat tttttagtca tcaaaaaatt tgaagaacta ttgacagaac
     1021 agacagatag attataactg agtccagata caacaaaacc agcttttggc tggttttgag
     1081 ggacttaaag aagtggggac ttctgaaagt cttgtaatca agtgaaatca gcactcgatt
     1141 acgatatgta agtgaaaatc taacttataa aatcgcaatt gtcaaacttc tcttattaca
     1201 agtctcttca aaaatcctca gtttttcaaa tccttaagca accattgtgc ctattgtgtc
     1261 aggtcgtgat ctgtggaatc aagagggtcc ggatcaatgt tggttgtcgg caatagaagg
     1321 gtgcttacga attgccaacc tgggaggctg agttctaagg ctattttggt gaacagtgtt
     1381 ttatatggtc ggctatgaga aagcccagcc agaacctgaa aaggttcaaa aatggggtta
     1441 actgtcacaa gtaaaagcta tgaactgtat ctagagggtg taagagggtg ccattagcga
     1501 gaaattgcta acaaactcaa tagtctagaa ctttgtggtg aagtctaaaa ctttgcgatc
     1561 acacaaatag cccttactga tttttttgaa ataatcctta ttttttatgg gattaggatc
     1621 aaaaaatcgg taggggcgac ctttgggtga agccccaaaa gggctgaacc caaaggtcga
     1681 gcgaagccac agaaaaacaa tacagatctg ttatagagaa aaagcaatgc tgatctgtag
     1741 aaaatagaaa aacaatacta aagaataaaa gaaaaagagt tcggagaaaa aaattcgaaa
     1801 tttgattttt tttgcgattt gctatttaaa ttctaaatat aaaagaaaaa aggaaaaatt
     1861 atggaaatag aaaaattgat agatagatat tttttagcat tagaaagaaa agtgatccgt
     1921 gaaatttatg ataaacagac acgagaagaa ttagaaaaat ttatttttaa gtgtaatggt
     1981 gagtttagag agtttgaaag gcgatttaat acaatatgct ctactgaata ttatgaacaa
     2041 caaagcctta tcttttatat taattatata aatgcagagt ttgagcagag ggagaagtta
     2101 aattattaaa tttgacaaca ttaaaataat atattaagca tatatgtatt agcgaaacac
     2161 agagggctta atatatgaca atatctaaga aaaatagcga attgagagag cgattcaaag
     2221 agtattcaag taataaaaac atatttgatc ttgctgattt gagatatgaa atattaaaaa
     2281 tatattttga ttttaaatta aaaaacgata tgaatgagca agaaagaaaa tctcaagact
     2341 caagaagaaa agcacattta acagcattaa aaaaaagaat taaaagagaa atcgtttcga
     2401 aaatagttat tgatttagta aaatactaca acatagaaaa aacgactttt catttttttt
     2461 ctcatatatg tactgagatt ttagaaagaa atgtagataa cagatacatt ctaaataatt
     2521 tttcaaacat gattttagac gaaaaaaaag agttaacgaa attaagtgaa agcagaaatg
     2581 cttcaaacaa aatgagttta gaaaactctt attccgaact tgtttctatg tcacatatta
     2641 aagacaaatt atttaggaat gacaatttca aaactgcata tttaaaatgt tatggttgtg
     2701 caaatgagga attttcaaga tttaaagttt ttgcttttcc agacaatttt gagacattag
     2761 attttttatt tgaagaagaa agaataaaaa aagaagaaaa agagatatct aaaataatga
     2821 ttgaacaagt tgaagaagaa ccaaaaatac agccaataaa gaaaagaaga ttataatttt
     2881 tttttgcggc gaatcgtgag aaaaataaaa ttttaatttt gttttttgag cgattgatct
     2941 gtatgtaagt tgatgaaaaa tctagtaaaa tctgcgatct attttttctt ttgacttgcc
     3001 attattgtta tttttgttat aacaataatg gggggttgtc ttttacttga aaaatgagcg
     3061 tcagcgaaat tttgaaagga aaagtgtgcg aagagcacaa aacccttttg ctttttttcg
     3121 taatgacctt taaatttttt ttcattagag cgaagcgatt ttgaaaaata aatttattgg
     3181 gaatgaagaa aaaaggcaaa ccagcggagt gaaattaaaa caataaaact ataaaaaaaa
     3241 gaggtggcta tggaatttaa aataaagaaa atgactgtta aagacagcag agaaattaaa
     3301 aaaaaatttg aagattgcat taagatagca gaagaaaaaa atatgcaagt aggttcaatt
     3361 ctaaatattg aaatgcaaga tagattgaaa gaaataaatt taaaagtaaa aaaagaaaca
     3421 gcagaacaat atagaaatct gtttaattat aaattgtctg tgaatgcaga agatattatt
     3481 cagcacttgt tagaatgcaa cacaagaaac agttttgata aaacaagatc tgcttttaga
     3541 ttttgcattg ctgaaaaaat tcaagagcta agaaatgaag cagatcgagc aagaaagcaa
     3601 aagaattttg atttaatgaa agaaaaaact attgaagcat ataaattatt tttctttttt
     3661 gagcgagatt ttttgagcga aaaccgggta acttggaatg atatttctta tagaaaaata
     3721 gact
//
