LOCUS       NZ_CP056352             8329 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00678 plasmid pRHBSTW-00678_3, complete
            sequence.
ACCESSION   NZ_CP056352
VERSION     NZ_CP056352.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148682
            Assembly: GCF_013738915.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00678
  ORGANISM  Citrobacter sp. RHBSTW-00678
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00678
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.3983658811909x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:01:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,445
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,147
            CDSs (with protein)               :: 5,147
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 52 of 181
            Pseudo Genes (incomplete)         :: 134 of 181
            Pseudo Genes (internal stop)      :: 33 of 181
            Pseudo Genes (multiple problems)  :: 32 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056352.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Citrobacter sp. RHBSTW-00678"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00678"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742661"
                     /plasmid="pRHBSTW-00678_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1302..1592
                     /locus_tag="HV265_RS26930"
                     /old_locus_tag="HV265_26940"
     CDS             1302..1592
                     /locus_tag="HV265_RS26930"
                     /old_locus_tag="HV265_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_142431636.1"
                     /translation="MKPSLELEKHREAIRELVHKYGLHNPRIFGSVAKGTDTEVSDLDL
                     LVDAGKKTSLFDMGGLHEELSALLSCPVHVLTPNEIRTDIRVKVLFYAIPL"
                     /name="nucleotidyltransferase family protein"
     gene            1899..2540
                     /locus_tag="HV265_RS26935"
                     /old_locus_tag="HV265_26945"
     CDS             1899..2540
                     /locus_tag="HV265_RS26935"
                     /old_locus_tag="HV265_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019282081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="WP_044864689.1"
                     /translation="MTIPMQPKIYHIVHVDRLASILASNGLLCDARIIAQGAAGTTIGM
                     NSIKQRRLTELTLSSYPDLYVGQCVPFYFCPRSIMLYVIHRADSDELAYKGGQGPIVHL
                     QADLHSCVKWAQKNQRRWAFTLSNAGSYYFEDKNNLSDLNQLEWSAINATHWQRCKEGK
                     QAEFLMEDSFPWHLIEAIGVQSKAIYTQVVNTLKSAAHRPPVSINTHWYY"
                     /name="DUF4433 domain-containing protein"
     gene            2554..3594
                     /locus_tag="HV265_RS26940"
                     /old_locus_tag="HV265_26950"
     CDS             2554..3594
                     /locus_tag="HV265_RS26940"
                     /old_locus_tag="HV265_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_044864690.1"
                     /translation="MIEITRGDILRADAEAIVNTVNCVGVMGRGIALQFKKAWPDNFKA
                     YAIACKKNEVHPGKMFIFETGQLTNPRFIINFPTKRHWKGASKIEDIESGLASLVGDIQ
                     KMGIKSIAIPPLGAGLGGLEWPLVCEKIKHALQPLSDVHVFLYEPSGAPQNDKMAKQKE
                     TPKMTAGRAVLIELIQRYLKGLLDPSISLLEVHKLLYFMQEAGEPLRLKYQKAHYGPYA
                     QNLRHVLNALEGHFISGYADGGDFPEKELQLVPGAIEEARDFLEKHPSTRQRFERVSRL
                     VEGFESPQGLELLSTVHWVNKHEQVKNSKDVIEAIYSWNDRKRQLTARQISIAEKVLDR
                     QNWLTA"
                     /name="macro domain-containing protein"
     gene            complement(3725..4288)
                     /locus_tag="HV265_RS26945"
                     /old_locus_tag="HV265_26955"
     CDS             complement(3725..4288)
                     /locus_tag="HV265_RS26945"
                     /old_locus_tag="HV265_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044864691.1"
                     /translation="MRALSIPPSVARASLASKFSSHLKVISLFNSMQDSQVVVLSSLIE
                     GHHLTSSGNNVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTVQTVYLIE
                     PEHIEQLFTDPELVFANQERTLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLIHHAYKEK
                     PLSEEMWLDIEEKFIHMLDELNAA"
                     /name="hypothetical protein"
     gene            complement(4546..4956)
                     /locus_tag="HV265_RS26950"
                     /old_locus_tag="HV265_26960"
     CDS             complement(4546..4956)
                     /locus_tag="HV265_RS26950"
                     /old_locus_tag="HV265_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044864692.1"
                     /translation="MAFISLSNLAAKTGRVVQSQHVTATQTKKSTGAVELGFRLSKSAM
                     DETGIAIGDRVDILYDKDENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEDENKKLLSDDSITQLSEGVIIFRLGDE"
                     /name="hypothetical protein"
     gene            5627..6178
                     /locus_tag="HV265_RS26955"
                     /old_locus_tag="HV265_26965"
     CDS             5627..6178
                     /locus_tag="HV265_RS26955"
                     /old_locus_tag="HV265_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127786104.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKNADYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLAWKKFHPSSPSVPNTVVPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            complement(6377..6592)
                     /locus_tag="HV265_RS26960"
                     /old_locus_tag="HV265_26970"
     CDS             complement(6377..6592)
                     /locus_tag="HV265_RS26960"
                     /old_locus_tag="HV265_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_164846815.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLHRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6596..7081)
                     /locus_tag="HV265_RS26965"
                     /old_locus_tag="HV265_26975"
     CDS             complement(6596..7081)
                     /locus_tag="HV265_RS26965"
                     /old_locus_tag="HV265_26975"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_053521196.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<7116..7820)
                     /locus_tag="HV265_RS26970"
                     /old_locus_tag="HV265_26980"
                     /pseudo=""
     CDS             complement(<7116..7820)
                     /locus_tag="HV265_RS26970"
                     /old_locus_tag="HV265_26980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7759..8082)
                     /locus_tag="HV265_RS26975"
                     /old_locus_tag="HV265_26985"
     CDS             complement(7759..8082)
                     /locus_tag="HV265_RS26975"
                     /old_locus_tag="HV265_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176673556.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGQDRVQVVAALMAIDAGLERLRNAVLEKGED
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          16..506
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.282"
                     /coverage="94.0"
                     /evalue="1.33e-138"
     Typing          complement(6274..7769)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.986"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaaatgcc agtgccggtg aaatgacgcc tcctcgctca ctcggtcgct acgctcttgc
       61 cgtgagactg cggcgggcgt tgccagctaa cggatgacgg gattaagcgt gtaacgctca
      121 aatcaggaga ggctggaaaa aggatgcggc gttgccgttt ttccataggc tccgcccccc
      181 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggacttaa
      241 agatccccac cgtttccggt gggtcgctcc ctcgtgcgct ctcctgttcc tgcctttcgg
      301 tttaccggtg tcattccgct gttatggccg cgtttatctc attccacgcc tgacactcag
      361 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
      421 ccactgcgtc tcacccggta actaacaaac atgatcccaa cccggatgga cacgactaat
      481 cgccattggc agcggccaat ggtaacgggc ttgagagagc ggaaatttat aaccgagtgg
      541 ttacagagtt cttgaagttg tagccttact acggctacac tggaaggacg gatttggtaa
      601 ttgcgctctc tcgaagccag ttacctggtt aagcagttcc ccaactgact taaccttcga
      661 aaaaaccacc tccccaggtg gttttttcgt ttcaggggct aatttacgcc gcaaccacgg
      721 atcgcgtcaa gataactctc cagcaaaaaa ccttttcaaa acgccaagtt aagtatagaa
      781 acagccttca aacaactata gtaacagtct ctggagagtc tagtaacagg acactatgag
      841 attaaacgcc gccaccaaga ctgtttttta tttgatgttt cagcgacttc cgtcttgtcc
      901 tctatgcgtc tcacctcctg caattcgttc cttagcccgg aaacttcatc acgaagggat
      961 tttatttcac gaataagttc actcattacc tgattgtgtg tctcatcact atgtctctca
     1021 gtgtcccgtg acactgtccc ttcttgtctc aactctccat agactcggat taactcgctt
     1081 gtatcaatgg agcgcccacc gtccgaatca gtcctgtatg acaagcggcc tgatgacata
     1141 tcacggtaca gactggaacg agctttcccc gtcaatttct gtgcctgtct caccttatgc
     1201 caagccataa gtatcacccg tcccactatt gtctcacgca attgatttac cgatagtatc
     1261 atcaacacag tattcagccg ccacataaca ggacaatcgt catgaaaccc tcacttgagc
     1321 tggaaaaaca ccgcgaagct atcagagagc tcgtacataa atatggtctg cataaccccc
     1381 gtatttttgg ctctgttgca aaagggacag atacagaagt cagtgatctc gatttgctgg
     1441 tcgatgcagg aaaaaagacc tccctctttg atatgggagg attgcatgaa gaattaagcg
     1501 cactattgtc atgtccggtt catgtcctaa ctcctaatga aatcaggact gatatacgtg
     1561 tgaaggtact tttttacgct attccactct gaccctaagc agaagctatc ccattaattc
     1621 aaaatgataa ttttcagact ggaaaatcta ggccattttt cgcctgcatt acggtcaatt
     1681 ttacaaacat atgcgatgat aatatgcagc ctggttgcgg ctaatgagca gcaaagcagt
     1741 tggtttgaag tccaaaccat gcaaagaagg aagcttttaa cctcacatat catgatgagc
     1801 tggtcatagt aggtaaggtt ttagagagct aacaatacgt ataaattaac gaacaaaaca
     1861 aaaacttata taataaaaat catggagaag ggtgctaaat gactatacct atgcagccta
     1921 aaatttatca catagtgcat gtagaccgac ttgcgtcgat cctcgcttca aatgggctgc
     1981 tttgcgatgc gcgtatcatt gctcaaggcg ctgctggaac gacgattggt atgaatagca
     2041 ttaaacagcg tcgcctcacc gagttaacac ttagtagcta tcctgattta tatgtaggtc
     2101 aatgtgtgcc cttctacttt tgcccacgat caatcatgtt gtacgtaatc catcgtgcag
     2161 atagtgatga gctagcttac aaaggaggac aaggtccaat agttcatctc caagccgatc
     2221 ttcactcttg cgttaagtgg gcgcagaaaa atcaacgccg ctgggcattt actttatcca
     2281 atgctggctc ttactacttt gaagataaaa ataatttgtc agatttaaat caactagagt
     2341 ggagtgcgat taacgcgact cactggcaaa gatgtaaaga agggaagcaa gccgaatttc
     2401 tcatggaaga tagttttcct tggcatttga ttgaggcaat tggtgttcaa tcaaaagcaa
     2461 tctatacgca ggtggttaac acattgaaat cagcagcaca tagaccacca gttagtatta
     2521 acactcattg gtattactga ggaggccgta aaagtgatcg agataacccg tggtgacatt
     2581 ttgcgagctg atgcagaagc tattgtaaat acagtcaatt gcgtaggagt tatgggtagg
     2641 ggaattgcat tgcaatttaa aaaagcttgg cctgacaatt tcaaagctta cgcaattgca
     2701 tgcaagaaaa acgaagtgca tcctgggaag atgtttattt ttgaaacagg gcaacttacg
     2761 aaccctcgtt tcataataaa ctttccaact aaacggcatt ggaaaggcgc tagcaaaata
     2821 gaagatattg agtctggttt agcctcctta gttggggata tacaaaaaat gggtattaag
     2881 tcgatagcca ttccaccgct gggtgctgga ttaggggggc tcgagtggcc cctagtttgt
     2941 gaaaaaatca aacacgcatt acagccatta agtgacgttc atgtttttct atatgagccg
     3001 tctggtgctc ctcaaaatga caaaatggca aaacaaaaag aaacaccaaa aatgaccgca
     3061 ggcagagcgg tattaataga gcttatacag cgctacctca agggattgct tgatccctca
     3121 atatctctac ttgaagtaca taagctattg tattttatgc aggaagcagg ggagccatta
     3181 cgcttaaaat accagaaagc gcactatggc ccttatgcgc aaaacttgcg tcatgtacta
     3241 aacgctcttg agggtcactt catttctggg tatgccgatg gcggtgactt cccggaaaaa
     3301 gaattacagc ttgttcctgg agctattgaa gaagctcgcg attttttgga gaagcatcca
     3361 agtacacgtc agagattcga gagagtatcg cgtcttgttg aaggatttga gtccccacag
     3421 ggactagagc ttttatcaac agttcattgg gtaaataaac atgaacaagt gaaaaactct
     3481 aaagacgtta ttgaagccat ttattcttgg aatgatagga aaaggcagct aacagctaga
     3541 caaatatcta tagccgaaaa ggttctcgac aggcaaaact ggttaactgc ctaactattt
     3601 tcaaaaactt aggtgctatt tactaaaata tcttttcgcc tacaaaaaaa ggatcgctca
     3661 ccaggagcga tccttttcac atctgaaccc gctatactgc agggttgaat gtaacgtccc
     3721 ttttttacgc tgcgttcaat tcatccagca tatgaatgaa cttctcttcg atatctagcc
     3781 acatttcttc tgaaagaggt ttctctttgt aagcgtgatg tatgaggctt aaaacagctt
     3841 gagacacact gccctttttc tcgataagct tgccaaggtt ttttccctct ttttctagag
     3901 aacgaaagaa aagagtcctt tcctgattag caaacacaag ttcaggatca gtgaacagtt
     3961 gctcgatatg ctcaggctct atgagataga cagtttgaac agtcatcctt cccgtagtta
     4021 ctgatttaac agagacattt agatgacgaa tagggaaaaa atattttttc tctagcatct
     4081 cgatgattgc tggtaagcga gtgacttcaa agtcagcttt aacattgtta ccagaactgg
     4141 tgagatgatg gccttcgatc agagatgaca ggaccaccac ttgggaatcc tgcatcgagt
     4201 tgaagagcga gataactttc aaatgagacg aaaatttact cgctaggctg gctcgggcaa
     4261 ctgagggagg tattgataaa gcgcgcatat gtgtagcctc atatatgttt aaaaacataa
     4321 agttaagaga ccaaagtcaa tagcaccgca cctaacagaa tagaaaatgt cgtgttcaca
     4381 ctcatcaaaa gaaatgattt cttctttatt agaatgcaag tttatgaaca taatgccgcc
     4441 cataacactc cacccttcca aaggtagtga tgtgttgacc agaaggcgga gttagtcgat
     4501 tgtttggcga cgttcactaa ctctgccttt cctttttggt ctttcctatt catcacccag
     4561 cctgaaaatg attacacctt cacttagctg agtgattgaa tcatctgaaa gaagcttttt
     4621 gttctcatcc tcatcctccg aaagacgcgg aaaaccagga cgtaaggtca atctaacagc
     4681 acatgatgag tacccaccat gcttgttttt ttgcccagaa acaccaaagc caccctcact
     4741 aattttctta atcatccatt ggttttcgtc tttgtcgtaa agaatgtcta cacgatcccc
     4801 aatagcaatt cctgtctcgt ccatagcgct tttactcaag cggaagccta gctccacagc
     4861 gcctgtgctt tttttcgttt gcgtggctgt aacatgctgc gactggacca ctcttccggt
     4921 cttcgctgcc aaatttgata aagagataaa agccattagt gcaacccgtt ttaaacatcg
     4981 atcagttctt gcacaacagt gtaaccaggg atcgctgcgg acttcaagat ctttttgtac
     5041 tacctctaac gagaccccat gcaaccacaa tttcaacccg taaaatttca aataacttct
     5101 cgtagtagat ctcgtcaagc tatgctgcca gatcgatctt tatgtaatta aaggctattt
     5161 tttgcttcag atttaaatga gaacagcgtg agcgcatata gctaaatcat cagataggca
     5221 taaaaaagcc gtctatatag acggcttttt tatacatgca gatctgctcc catttaccat
     5281 aacatccctt taccgcaccg ctccggacgg gttcagcgtg aaagcggcgt tagcccggta
     5341 aaaccggcag taaactcccc gctaaaccgc gcattctgcg cctgataaat ttcacgtggc
     5401 tgcatagcca tgcagccacg tgaatggctc atgctttcaa tgtatatcct cgtccaagcc
     5461 cctgttttaa ccggtttatc aaaaaaacca aacaggaagt cttaccacta acaataaaag
     5521 aggtaggggg attaccttct atcgcataaa aaacatttac aacataaatt ctatcgtata
     5581 gattacataa aaaaatgaaa ttcattcacc tattaagggt ttctatatga aagatatgaa
     5641 agctgttgtg gtttttaccg ggaaggattt gaacataatg agaactgagg ggggatcagg
     5701 ctactggcat gccagaacag acaggctcaa cgatgccgat tacctcatag cagtaagaaa
     5761 cagaagggaa acgtgggcag taaaagacct ggaacacggc acagcattcc tgatcgccaa
     5821 aattaccggg tgttttaaaa acgctgatta cgacgacagg aacgtcataa cctttgatga
     5881 atacgcagaa attcacactc caaaagcatg gaaaatgcta accgatggtc agcgctatcc
     5941 ggttgcttac ctgagcgcac aggaagcttt tttacgtatt ggggtaacac cggaacaact
     6001 ggcatggaag aaattccatc cttcatcccc atcggttcct aatacagtgg ttcctggtct
     6061 ggctgaagaa aaaacagaaa aactctccct taacgaggca attgaaagag ctaaaaagga
     6121 tattagcaac gcaacaggga ttgatagctc agccataaca ataagcatca agatctgatg
     6181 aagcgtgaac aacaagatag cagcctcatg ttctagaact gaacctgtgc gccagaaaca
     6241 gcaagcccat gcttttccgg cgcacagatt caaagtgacg gaccgttgta cgttcgctgt
     6301 cgctgagatc gttccagctc cagcgctttc tgatgctgcg ccgcccgttc ctgctcacga
     6361 tgtgcctcta tcacgttcag ccttgtgaca accggtgcag ctgctcgctc aaactctgca
     6421 cctgcctgct caagcccgtg acgcgctcgc tcagcgccgc gttctcccgt tgcataagac
     6481 cagacatcgt ccgccattcc gcgaaggcgt tctcccactc gtccagcctt ttcgagtagt
     6541 cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctctgtc atgtgctatt
     6601 tccccgccag tatcatccag ctcgaatcct ctccgaaccg tcccgcttcc gggttcaccc
     6661 tcacgcagcg gcgtctctgc tctccgcagg tcgagagctg cacgccgctg ttcctctcgg
     6721 acagcatggc ctgcgtgctc ttctgctccc ggatggtcgt gtaattgtcg agtatctgct
     6781 gcccctgcca ccacagaatg cctgcgcccg atgctgtcag cagtgcagac accaggacga
     6841 tggtcaacca cgtctggctg accatacgca ccatgccttt cgtgcgctgg ctcatggctg
     6901 aggacagctt ccggtcgtgg tcgaggatgg cggcgctgat tctcttctcg ctttcgctca
     6961 gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     7021 tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa agactgttca
     7081 tagatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatgctg
     7141 ttttttgtgg tgcgtaccac ctcaaaacct gcgctttcca gcgcctcagt gacgtcctgg
     7201 cgggttttca gctccccgga cgaggccaag gccagtaaac cccgtgtaat tgcctgagcg
     7261 gcttcctgct tcgtttccgg cagcgcggac ggcgtgacca gcgcccgccg gttttccggc
     7321 gcgttcgggt cgtgcagccc cagtctgccg ttcacaatgg tctgccaggc atcgatgcgc
     7381 ggacggtcgg cgcggtcgta atacggctgg agacgtttgc cggtcagcag ctccgtgttc
     7441 gggatcagga agttcagctc cagccgcccc ttgtccctgt gctcaaccca cagcacgctg
     7501 tactggtctt tatcgagtcc gggcatcaga acccgctcga agctcgccat cagcttttca
     7561 cgctgaccgg gcggtaaatc ctgttcggca aaggacagga cgccggaggt atactttttg
     7621 gcgtagggtg aggcgtcgat aagctcccgg acttcctccg gtttcccctg caggacgctg
     7681 gctccgtcgc gctgccggtc tttccccagc agataatcca ccggaccggc gccgccaccg
     7741 cgcccacggg gatgaaactt aacgatcatc gtcttccccc ttttccagca ccgcgttccg
     7801 cagccgttcg agtccggcat caatggccat cagcgcggcc acaacctgca cccggtcctg
     7861 gccggtacca ccgccggcgt taacccggcg ggctatctgg ttcaggttat tgcccatgcc
     7921 ggcaagctgg cgcaacaacg ccggcgaaat cgagggaagt ttgccggccc gggcgggctt
     7981 ctcatccaga cacgtctgcc gcatccaggc cgcgagctgt ttaccgtcgc agcgctccag
     8041 cagacggcgg tgctcatctt ccgttaccca catcgtgagc attttgctgc gtttatgtgc
     8101 caatccggtc tcctgataac accgggacgg cgggaaatct tttcccggac ggcgcggtgt
     8161 tggacaagcc gctggcgcgt cagtggcttt tggcgggttt cggggcgcag ccctgaacca
     8221 gtcacgtagc gctagcggag tgtatactgg cttaatatgt tagatcggag tggtaattca
     8281 gggaagtgct tcatgtggca ggtgaaaaat gcaggtgcta agtgtaagt
//
