LOCUS       NZ_CP056346             9581 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00696 plasmid pRHBSTW-00696_6, complete
            sequence.
ACCESSION   NZ_CP056346
VERSION     NZ_CP056346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148686
            Assembly: GCF_013733095.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00696
  ORGANISM  Citrobacter sp. RHBSTW-00696
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00696
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.1206555606231x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,690
            CDSs (total)                      :: 5,572
            Genes (coding)                    :: 5,410
            CDSs (with protein)               :: 5,410
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 42 of 162
            Pseudo Genes (incomplete)         :: 125 of 162
            Pseudo Genes (internal stop)      :: 24 of 162
            Pseudo Genes (multiple problems)  :: 25 of 162
            Pseudo Genes (short protein)      :: 1 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056346.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Citrobacter sp. RHBSTW-00696"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00696"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2742662"
                     /plasmid="pRHBSTW-00696_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(8541..9581,1..69))
                     /gene="mobA"
                     /locus_tag="HV269_RS28255"
                     /old_locus_tag="HV269_28245"
     CDS             complement(join(8541..9581,1..69))
                     /gene="mobA"
                     /locus_tag="HV269_RS28255"
                     /old_locus_tag="HV269_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_061351404.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISRDGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQSILARREAERQAQQSRDERDCVIDVTTSLREAIHERDDASVKPAQQPEPEVA
                     AGITFDFEKEPDKLNALVSDAMKDVQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLA
                     EKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            234..701
                     /gene="traD"
                     /locus_tag="HV269_RS28260"
                     /old_locus_tag="HV269_28250"
     CDS             234..701
                     /gene="traD"
                     /locus_tag="HV269_RS28260"
                     /old_locus_tag="HV269_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016517641.1"
                     /translation="MAKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTA"
                     /name="traD"
     gene            1296..2252
                     /locus_tag="HV269_RS28265"
                     /old_locus_tag="HV269_28255"
     CDS             1296..2252
                     /locus_tag="HV269_RS28265"
                     /old_locus_tag="HV269_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_039899550.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     gene            2260..2709
                     /locus_tag="HV269_RS28270"
                     /old_locus_tag="HV269_28260"
     CDS             2260..2709
                     /locus_tag="HV269_RS28270"
                     /old_locus_tag="HV269_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016517644.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2945..7822)
                     /locus_tag="HV269_RS28275"
                     /old_locus_tag="HV269_28265"
     CDS             complement(2945..7822)
                     /locus_tag="HV269_RS28275"
                     /old_locus_tag="HV269_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_058655487.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLTTADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     NSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPAFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGADGVHPDAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTGRMEVIAVADKVVKKTKRQTKKS
                     EIAGKARKSSGIGSAVSPAPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIRGILENPGNKIEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKKEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHSITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIVGNPPYSAGQGSA
                     NDNNANISYPGLDKRIKDTYALNSKATSTKNLMDSYIRAIRWASDRVGEQGVIGFITNA
                     GYIDSNSTDGLRKCLIDEFSSIYVFHLQGNSRRSSGELAKKEGAGIFEIRTPVAITILV
                     KNRERKSKGELKFFKIGDYLSREEKLEFISKVSSVDGAEIKSKWESIVPDSYNDWVNQR
                     DDSFYEFIEIGNKKNKKSTSLFLNYSLGVGTNRDAWVYQSSRSKLVDNINRTISFYNEN
                     LSSGVEPIFDVKSISWSSSLISAFSSGKKIKFEPLNIRESLYRPFQKQWLYFGGGLTHR
                     VGQFPKIFPIGGDNLIIAVTGLGGKKGFSVLISNCLMDLNLMDAGSQCFPLYLYDDESS
                     LSDDVGFLNATEKKRKYGISDEGLNYFSSHLNDMAITKEDVFYYLYGLLHSQDYRNRYS
                     DNLTKELPRIPCVKNINDFWAFSDAGRKLAGYHLNYETVEPYKANIDTGLISYSQLGKD
                     DFYVEKMKFAKKGEKDTVIYNNKIRIKNIPTEAYDYVVNGKPALEWVMERQGVSTHKDS
                     GIVNDANDWAVETMNNPRYPLELFLRIITVSLETQKIVNSLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(7935..8528)
                     /locus_tag="HV269_RS28280"
                     /old_locus_tag="HV269_28270"
     CDS             complement(7935..8528)
                     /locus_tag="HV269_RS28280"
                     /old_locus_tag="HV269_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_016517639.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     Typing          1297..2247
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.271"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8545..9581)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.306"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtaatcg gcatggggag aggcactgcc tttcccgccg gtttttacgc tgagatgata
       61 agatgccatc gtgttttatc ccgctgaagg cgcgcaccgt ttctgaacga agtgaagaaa
      121 cgtctaagtg cgcactgata ataaaaagct tatcagtgat tctacctgca tttgacctcg
      181 gccgccattc ctgaccataa tttccacatc aacattcaga ggataaaaca gtcatggcta
      241 aaagcgaaaa acaggaaaaa tgggcagcag acagagtgca gtacattcgc ggcttaaagt
      301 cgccgaatga acagcagaaa ctcatgctga tactgacgga taaagcggat aaaacagcgc
      361 aggatatcaa aacgctgtcc ctgctgatga aggctgaaca ggcagcagaa aaagcgcagg
      421 aagccagagc aaaagtcatg aatctgatac aggccgaaaa acgggcagaa gccagagccg
      481 cccgtaaagc ccgtgaccat gcgctgtacc agtcagccgg actgcttatc ctggcgggtc
      541 tggttgacag ccagacgggg aagcctgttg atgataccgc tgccttgctg ggtgcattag
      601 ccagtctgaa tgacctgtcg agggataatc cgaagtggtc agactggaaa atcagaggtc
      661 aggaactgct gaacagcaag aagtctgaca gcaccgcata gcagacccgc cataaaacgc
      721 cctgagaagc ccgtgacggg cttttcttgt gtcatgggta gatttccttg catgaatcca
      781 taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa
      841 ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat
      901 tcagcgattt gcccgagctt gcgagggtgc tacctcattc tttcgtgttt gaaggtatgt
      961 tttttggagg agcaaacgct gtttgcgaca tccttttatc atatcgctca attggcactg
     1021 gcggttagtt atccacaaag ctgggatcta ttctttttta tcttttttat tctttcttta
     1081 ttctagtgat tttaatgttt tgttttataa gtaaaaaata catgaaaggt taagtgaaaa
     1141 ttaggaaagg ttaagcaaaa attaggatct cctttcaaaa ggttaagtga aaattaggta
     1201 atccacacaa ccacataaaa ggttatgaca atgaaattga cataaccaat cggaagggtt
     1261 atgttgtttt aaaatcacaa ccaatggttt tagtcatggc tgaattggta gtattcaaag
     1321 caaacgagct tgcggttagt cgttatgacc taacagagca cgaaacgaaa ctaatcctgt
     1381 tttgtgttgc aaagctaaac cctacattag aaacaccaga tgaaactgaa cgaacggtta
     1441 aattttcatg ctccgaatat tcttcattta tggggataag ttatgagaat gcgtgggggc
     1501 gattaaatac atcaacacga gagttattta agcggtctat tgaattaata tatccaacag
     1561 gtgctgtatc tatgcgtgtt tttaattggg ctgaatatgc agagttcaat agggaaacac
     1621 aagaggtaac tttagttttt agtaaatata taatcccatt actatttcac ctaaagagat
     1681 tcattaaata caacctcgag catgttaagt cctttgaaaa taaatactca atgcgcattt
     1741 atgaatggct gctaaaagaa ctaacgcaaa gaaaaacaca taaagccaat atttcaataa
     1801 gcattgatga gtttaaattc atgctaatgt tagaaaaaag ctacccagaa tataaattac
     1861 taaatcattg ggttttaaaa ccaatttcaa aagatttaaa tacctatagc aatataaaac
     1921 tatctattga taagcgcggt cgtcctgctg atacgctgat cttccaagtt gaactggata
     1981 aacaaattga ccttgtgact gagctagcaa aagaaccaat atcaaaaaaa gaagataact
     2041 caatccgttt aacgcctgaa aatcgtctgc atgaggggct aaaaacaaca ttacatgatg
     2101 ctttaactgc gaaaatacaa cttactggat ttgaagctaa attcctgagt gacatgcaga
     2161 gtaagtatga tctcaacggc tcatttacat ggctgactca aaaacaacga gccacgctag
     2221 agaaaatttt agctaagtac ggacggaact gaggtatcta tggctttagt atcaataagc
     2281 gaagcagcca gactaactgg aaaaagccgc acaacagtac agtcatacat taagcaggga
     2341 aaactgacca agtgcacaga tagcggtggt gcttcaaaac tagacacatc agagcttcta
     2401 cgtgttttcg gatcatttac tggtcagcag gttggcagtg aacaatcagg cagtactgtg
     2461 cagcatttgg caggcgattt tgtgcagtta cctgaccaga atttgctaca tgaaaactat
     2521 gccttaaaag ctgaaataga actcgtaaag gcattgctgc gtgagaaaga gctactactc
     2581 gatgaaaaag acaaacgcaa tgaggatctc aaacatgctt tgctgctaat cgagtctaaa
     2641 ctgccgaata catcggaacc agtgacacct catgtggtga aaaaatcatg gcaattctgg
     2701 aagaaatagc gttttcagcc ggcaaaccga ctgaaaccgg atctgcgatt ctgataacaa
     2761 actagcaaca ccagaacagc ccgtttgcgg gcagcaaaac ccgtactttt ggacgttccg
     2821 gcggtttttt gtggcgagtg gtgttcgggc ggtgcgcgca agatccatta tgttaaaaac
     2881 gacaggattc atgaaaatca tcaaaattgt gcaggatggt ttttccatcc tgcacaccta
     2941 tcgattagat atctaattta ggcaggctat tgacgatttt ctgagtttcc aggctgacag
     3001 tgataattct caggaatagc tctagtgggt atctagggtt attcattgtt tcaactgccc
     3061 agtcattagc atcattcaca ataccgctat ctttatgagt agagacacct tgccgctcca
     3121 tcacccattc aagagctggc ttgccattca caacgtagtc ataggcttca gttggaatgt
     3181 tttttatgcg aattttattg ttatagatga ccgtatcttt ttcacctttt ttggcgaact
     3241 tcatcttctc gacataaaaa tcgtcttttc ctaattgaga ataactaata agccctgtat
     3301 caatattcgc tttatatggc tcaactgttt catagttcaa atgatacccg gctaatttgc
     3361 gaccagcatc agaaaaagcc caaaaatcat ttatattctt tacacatggt attcttggca
     3421 gttctttagt tagattgtca ctataacgat tacggtaatc ttggctgtgt aaaagcccat
     3481 aaagatagta aaaaacatcc tccttagtga tggccatatc attaaggtgg ctgctgaaat
     3541 aatttaaccc ttcatcactt ataccatact ttcttttttt ctcagttgca tttaaaaaac
     3601 caacatcatc actcaatgaa ctttcatcat catacaaata aagaggaaaa cactgagaac
     3661 ctgcatccat aagattaaga tccataaggc agtttgaaat taatactgaa aaacctttct
     3721 taccgccaag acctgtaact gctattatta aattatcacc accaattgga aatatctttg
     3781 gaaattgacc gactcgatgc gttaacccac caccaaagta gagccattgt ttctggaatg
     3841 gcctatatag tgattcccta atatttaaag gctcgaattt tatttttttc ccactagaaa
     3901 acgctgatat gagactactt gaccaactta ttgattttac atcaaaaata ggctccactc
     3961 ctgatgacaa gttctcattg taaaatgaaa tcgttctatt aatattatca actagctttg
     4021 atctactact ttgataaacc caagcatccc tgttagttcc tacacccagc gaataattta
     4081 aaaacaaaga tgtgcttttt ttattttttt tatttccaat ctcaataaat tcatagaaac
     4141 tatcatccct ttggttaacc caatcgttat atgagtcagg aacaatcgat tcccacttac
     4201 ttttaatttc agcaccatca acacttgaaa cttttgagat aaattctaat ttctcctctc
     4261 gcgacaaata gtctcctatt ttaaagaact tcagttcccc tttgcttttc ctctctctgt
     4321 ttttcactaa aatagtgata gcaactggtg ttcttatttc aaaaatccca gccccctctt
     4381 ttttcgctaa ctcgccagaa ctccttcttg aattaccttg caagtgaaat acatatattg
     4441 aactaaactc atcaattaag cacttcctga gcccatcagt tgaatttgag tcaatatagc
     4501 cagcattggt tataaatcca attacgcctt gctcaccaac gcggtcactt gcccatcgaa
     4561 tagctcgaat ataagaatcc atcagatttt ttgttgacgt tgccttggag tttaaagcat
     4621 atgtatcttt aattctttta tctaagccag ggtaagatat atttgcgttg ttgtcattcg
     4681 cagagccctg accagctgag tatggaggat tcccgacaat gactcgaata tccagcgctt
     4741 tttgacgctt acggcgttca ctgttatcaa ccaaaacctc actgaccagg tcatctttct
     4801 catacatttc aaaggtgtca gtcagacaaa tcccttcaaa tggcgtgtac tctgattctt
     4861 ccgtgatgct gtgatacacc gcctcaatat taatggcggc aatgtaatag gctaacaaga
     4921 cgatttcgtt ggcatggatt tcatttttaa acttgtaagc cagtttatct gacgggataa
     4981 gaccactttg cagcaggcgg gtgataaacg taccggttcc ggtgaatggg tcaagaatat
     5041 gcacaccttt gtcggcgagt gtttgtccga actccttttt cagcacgtca ttcacgctgt
     5101 gaataataaa atcaaccacc tcaacaggcg tatacacaat tcccagacgc tcagtcattc
     5161 tcgggaacgc gtttctgaaa aacttatcgt acaactgtac gataatctgc tgtttacctt
     5221 ctgcggtggt aatgccgtct gcacgcattt ttaccgactc atagaagctg ttcagcgtgc
     5281 tggcttcttt ttcgagatga tgctcctgta acgtgttaag cacgttctgc atcgccatac
     5341 tcatcgggtt atggtcggta aaattgtaat cggcaaacag ggcatcaaag accggcttgg
     5401 ttatcagatg ctgtgccagc atttcaatga tttcatcgtc ggaaacactg ttattcagat
     5461 catcacgcag ttcatcggca aatgccctga aggcactgat ttctatttta ttccccggat
     5521 tttcgagaat gccccgaatg cggtcgatat gggtacgggc aattttagca atgtcgtttg
     5581 cccagtcctc ccagtgatgc cggttaccgc attttttaac gatttttgcg tacagggcgc
     5641 gctcaatctc accaacggaa aactcaatat caaactgttc aggagcgggg ctcacggctg
     5701 aaccaatgcc actgctttta cgcgcttttc ctgctatctc agattttttc gtctgccgtt
     5761 tggttttctt caccacttta tcggctaccg cgatcacttc cattctgccg gtatctttac
     5821 cgttaaactc cagcttgttg atcatggcat caaagcggtc atcatgcgcc ctgagtgcat
     5881 tcagcacctg ccagacaaca cggtaagcct gattattgtt caacgcattc tccggctctt
     5941 ctccggcggg aatgacaacc ggcagaatga cgtagcccag ctccttacct tcagcacgcc
     6001 gcatcacacg tccgacagac tgcaccacat ccacctgaga gcttctcggc gttaaaaaca
     6061 acaccgcatc cagagaaggc acatccacgc cttcagataa acaacggacg ttagacaata
     6121 tccggcagac attttcatcc gtgtccgctt tcagccattc aattttggat tttttctcag
     6181 tggcattcat gctgccgtca acgtgcttca cctgacactg aagttttaat gatgggtcaa
     6241 tcgccttatc cggtgcggtt tccctgagaa gttcgatctc ggaggcctga taagcctcaa
     6301 ccactgctcc gaacatttca gtgatcagtt tagaactgac cttatgcgtc tttccgcgaa
     6361 agtctttttc tatcacctga cagaacgcaa cagcccgttt catcgcatca gggtgaacgc
     6421 catctgcccc cgccagtccc tgctttgcca gtgctttcca gcaacccaca atctttgccg
     6481 catcatcgac tttgagctga ttattttcat ccttcagcag agactgaatg cggcggctga
     6541 catgcttttc ttccacggca agcacaataa ctttgtaatc acacaaaatg cccaactgga
     6601 cggctttaga gaaggtaatg acatacaaat catccccata aagacttttg tcatccattg
     6661 agcatagggt aactccctca gtctctttcg catcatctcc aaaaattcgt ggggtcgcgg
     6721 tcatgtacag gcgtttttga ccggcaatat aggcattgtc gtgaatacga acaaaggcgg
     6781 actcatcatc cccttcaaag gtcgcacctg tggtccggtg agcttcatca caaatgatga
     6841 aatcaaatgc aggaaaaccc agtttttgcg cctgatggat gacctcaaca gagtggtagg
     6901 tagaaaacac cacgttcata taaggcggct tatcactgcc gaagatatca ggctgattaa
     6961 acgctttgac gaggctgttc gcattcgtgg tcgccggata tttcaggtcg ctgataccaa
     7021 caacgacatt gtcatcatgg tttttcttac ccacatcact gtcagagcag acagcaaaac
     7081 agcgcaggtc tatcagcgta tcctgtgtcc attcatccag cgtctgactc aacaatgcca
     7141 gactgggcac aaggaacagt acggttttcc cccgtccggc aaccgcttca gcgatacgca
     7201 gcgaggtgaa ggttttccct gtgccgcacg ccataataag cttgccgcgg tcggcagtcg
     7261 tcagccctct tttcacggct tcaagcgcgg gagtctgatg aggtcggagt tgtttttttg
     7321 ctttcatcac cggtttgtag gccggatcaa aatcgaattg tgaccagtcc agtgcgcttt
     7381 gctcaaggtc tgtcaggctt aacgcagtga caggaacggc cttgtcccgc agcatatttt
     7441 tagcttcctc cgtccagtta tccgtagagg caacgatata gcggagcgta aaatacgttt
     7501 tatcggaggc agccagaaaa ctatcaatat cctttttggc gattttagtc tgattgtagt
     7561 ttttgcactg gatagcatga aactcccccg ttttggttac ggcgaccaaa tcaatgccat
     7621 catctttttt cttcgtgatc ccaacggttt caccgtactg agaaacccat ccgccatagc
     7681 tcagcacgtc actgtacagc tcggcatatt taggttcatg gaggaaatac tgcacacaaa
     7741 ggttttcaaa ggttgtacct ttgtcccgct gtgttggtga agcctgttca atttttgtca
     7801 gcaatgcaga aagtgcattc atcgttgata tccttaatgt cctttatttg ctggggttat
     7861 cagatccccc cgacacgttt aattaatgct ttctccgccg gagatcgacg cacagcgttc
     7921 tgtgctctat gatgttattt cttaataatc atccaggtat tctctttatc ccctttatca
     7981 ccatacgtag tgcgagtgtc caccttaacg cagggctttc cgtcacagcg cgatatgtca
     8041 gccagcgggg ctttcttttg ccagaccgct tccatcctct gcatttcagc aatctggcta
     8101 tacccgtcat tcataaacca cgtaaatgcc gtcacgcagg aagccaggac gaagaatatc
     8161 gtcagtacaa gataaatcgc ggatttccac gtatagcgtg acatctcacg acgcatttca
     8221 tggatcatcg ctttcgccgt atcggcagcc tgattcagcg cttctgtcgc cggtttctgc
     8281 tgtgctaatc cggcttgttt cagttctttc tcaacctgag tgagcgcgga actcaccgat
     8341 ttcctgacgg tgtcagtcat attaccggac gcgctgtcca gctcacgaat gaccctgctc
     8401 agcgtttcac tttgctgctg taattgtgat gaggcggcct gaaactgttc tgtcagagaa
     8461 gtaacacgct tttccagcgc ctgatgatgc ccgataaggg cggcaatttg tttaatttcg
     8521 tcgctcatac aaaatcctgt ctatcgtgag aatgaccagc ctttatccgg cttctgtcgt
     8581 atctgttcgg cgagtcgctg tcgttctttc tcctgctgac gctgtttttc cgccagacgt
     8641 tcgcgctctc tctgcctttc catctcctga tgtatcccct ggaactccgc catcgcatcg
     8701 ttaacaaggg actgaagatc gatttcttcc tgtacgtcct tcatggcatc actgaccagt
     8761 gcgttcagct tgtcaggctc tttttcaaaa tcgaaggtga tgccggcagc gacttccggc
     8821 tcaggttgct gcgccggctt aacggacgca tcatcccgtt cgtggatggc ttcccgcagc
     8881 gaggtggtaa catcaatgac gcagtcccgt tcatcacggg actgctgcgc ctgtctttcc
     8941 gcttccctgc gcgcaagaat ggactgtaac tgttcagtat cgaaccgctg aacctgaccc
     9001 gcgcccagat gccgctcagg ctcacggtca atgccctgcg ccttcaggga acgggaatca
     9061 acccggtcag cgtgctgata ccgttcaagg tgcttattct ggaggtcagc ccagcgttcc
     9121 cgctgggcaa caaggtattc tttgcgttcg gtcggtgttt ccccgaaacg tgcctttttt
     9181 gcgccaccgc gctccggctc tttggtgtta gcccgtttaa aatactgctc agggtcacgg
     9241 tgaataccgt cattaatgcg ttcagagaac atgatatggg cgtggggctg ctcgccaccg
     9301 gctatcgctg ctttcggatt atggatagcg aactgatagg catggcggtc gccaatttcc
     9361 tgttggacaa aatcgcggac aagctcaaga cgttgttcgg gttttaactc acgcggcagg
     9421 gcaatctcga tttcacggta ggtacagccg ttggcacgtt cagacgtgtc agcggctttc
     9481 cagaactcgg acggtttatg cgctgcccac gcaggcatat tgccggactc cttatgctca
     9541 aggtcggagt ctttttcacg ggcatacttt ccatcacgcg a
//
