LOCUS       NZ_CP056339            11845 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00697 plasmid pRHBSTW-00697_4,
            complete sequence.
ACCESSION   NZ_CP056339
VERSION     NZ_CP056339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148687
            Assembly: GCF_013738635.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00697
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.5090617777702x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,445
            CDSs (total)                      :: 5,326
            Genes (coding)                    :: 5,155
            CDSs (with protein)               :: 5,155
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 53 of 171
            Pseudo Genes (incomplete)         :: 121 of 171
            Pseudo Genes (internal stop)      :: 31 of 171
            Pseudo Genes (multiple problems)  :: 29 of 171
            Pseudo Genes (short protein)      :: 1 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00697"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00697_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1..879
                     /locus_tag="HV270_RS26980"
                     /old_locus_tag="HV270_26990"
     CDS             1..879
                     /locus_tag="HV270_RS26980"
                     /old_locus_tag="HV270_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_009873352.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLAPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERL
                     SDRGFDRAPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHTGGVLLPVELQEIKRESGN
                     KGGKGYDSAAY"
                     /name="helicase RepA family protein"
     gene            860..1852
                     /gene="repC"
                     /locus_tag="HV270_RS26985"
                     /old_locus_tag="HV270_26995"
     CDS             860..1852
                     /gene="repC"
                     /locus_tag="HV270_RS26985"
                     /old_locus_tag="HV270_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_009873351.1"
                     /translation="MTQQRIKPAYAKFDPAHVFDGLFVPTKGKKRGRLYVPPRRFGDSE
                     ISFQGFEQLGADDQSILLAVSAQLGIDGLMIEAKPPGEIGQQLRLALDFDNDDGRPLKT
                     KKTSLRSLLIDAGYDDPESGAALARIKESLNRMGNAQIREINHATGWDYRCNLLSSRFN
                     HKTGEIFVAVNPRLTGAVFREQHVKVSLFERNELESEAAKLLHCWLCSNIRLGRSLGNG
                     NGAHIDTLAPHVWGKAAWEEASTKVRSVRRGQLRDALDEISDRTRGLQGGMGWAIDQTS
                     SGLVLVSRPKELPFLEGEHDMRPSEYHDLMSQPPMQEPDLFDPQDWTPD"
                     /name="repC"
     gene            complement(1788..2468)
                     /gene="tetR(C)"
                     /locus_tag="HV270_RS26990"
                     /old_locus_tag="HV270_27000"
     CDS             complement(1788..2468)
                     /gene="tetR(C)"
                     /locus_tag="HV270_RS26990"
                     /old_locus_tag="HV270_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(C)"
                     /protein_id="WP_173782116.1"
                     /translation="MNKLQREAVIRTALELLNDVGMEGLTTRRLAERLGVQQPALYWHF
                     KNKRALLDALAEAMLTINHTHSTPRDDDDWRSFLKGNACSFRRALLAYRDGARIHAGTR
                     PAAPQMEKADAQLRFLCDAGFSAGDATYALMAISYFTVGAVLEQQASEADAEERGEDQL
                     TTSASTMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEQVRLSPASSPAGRTNLVLAL
                     AAGS"
                     /name="tetR(C)"
     gene            2561..3751
                     /gene="tet(C)"
                     /locus_tag="HV270_RS26995"
                     /old_locus_tag="HV270_27005"
     ARG             2562..3748
                     /name="tet(C)"
                     /gene="tet(C)"
                     /gene_name="tetracycline efflux MFS transporter Tet(C)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3655..3993
                     /locus_tag="HV270_RS27000"
                     /old_locus_tag="HV270_27010"
     CDS             3655..3993
                     /locus_tag="HV270_RS27000"
                     /old_locus_tag="HV270_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002310911.1"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFTT
                     PRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRISGSVGSWPRVRMIVLLSLRTRL
                     GWRGCLTG"
                     /name="hypothetical protein"
     gene            3998..4189
                     /locus_tag="HV270_RS27435"
     CDS             3998..4189
                     /locus_tag="HV270_RS27435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873362.1"
                     /translation="MNHRYASEREATAAAKRLRPEQQHEWSSVSVFRKVWKRGSPLRAA
                     EVARNREWNQPVIPRYYD"
                     /name="hypothetical protein"
     gene            4201..>4497
                     /locus_tag="HV270_RS27010"
                     /old_locus_tag="HV270_27020"
                     /pseudo=""
     CDS             4201..>4497
                     /locus_tag="HV270_RS27010"
                     /old_locus_tag="HV270_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5159..5911)
                     /locus_tag="HV270_RS27015"
                     /old_locus_tag="HV270_27025"
     CDS             complement(5159..5911)
                     /locus_tag="HV270_RS27015"
                     /old_locus_tag="HV270_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873360.1"
                     /translation="MRIKIKGEITAERLAEALHAAAEKYEAVRPGHKVYGANLYLTAFD
                     ADGLPFDLVDHRGEPLSITIEAKSGELVKPALTAEGEAHRQKAKEEARRQAEEAEAEAQ
                     RRHRQTLDEYEQERQKRRKKEAEARKQFEDANAITAELLKTMPERFIDELNKTVQGVWD
                     DLKPTETQGKKKGQPKALPVFSIHADGLVLSVETWKNPRRVLNPLCTLQHGEIAPFWMH
                     EAWLEAMRRIVDLLDTLTAAPAEALESQ"
                     /name="hypothetical protein"
     gene            complement(5908..6546)
                     /locus_tag="HV270_RS27020"
                     /old_locus_tag="HV270_27030"
     CDS             complement(5908..6546)
                     /locus_tag="HV270_RS27020"
                     /old_locus_tag="HV270_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6753 family protein"
                     /protein_id="WP_009873359.1"
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLMA
                     LQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVAHN
                     TSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAAWAN
                     TPEGRLAYRFAQTGSLASLAKCDRPGWYVEKGVCYVKPASDGTYGWRLP"
                     /name="DUF6753 family protein"
     gene            complement(6539..7222)
                     /locus_tag="HV270_RS27025"
                     /old_locus_tag="HV270_27035"
     CDS             complement(6539..7222)
                     /locus_tag="HV270_RS27025"
                     /old_locus_tag="HV270_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mobD"
                     /protein_id="WP_009873358.1"
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSNP
                     DVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINTAARNNKGVSAYGETLNS
                     TLEELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNGSKL
                     RTAVEERGGKSVTFPDMADRVSDDIYSKRMSIAVALKELPIGNRAELTRWRNEAKKVLE
                     AVVHE"
                     /name="protein mobD"
     gene            complement(7235..7591)
                     /locus_tag="HV270_RS27030"
                     /old_locus_tag="HV270_27040"
     CDS             complement(7235..7591)
                     /locus_tag="HV270_RS27030"
                     /old_locus_tag="HV270_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873357.1"
                     /translation="MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAALQ
                     KRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPAKKAPVQAPGDTPPPRPADKKPADT
                     SKATFTPKPDSDDI"
                     /name="hypothetical protein"
     gene            7907..8218
                     /locus_tag="HV270_RS27035"
                     /old_locus_tag="HV270_27045"
     CDS             7907..8218
                     /locus_tag="HV270_RS27035"
                     /old_locus_tag="HV270_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003869.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_009873356.1"
                     /translation="MPFETQGPEPLDAVINVRLTAAEKARLKEDADLAGLSMSELVRRR
                     YFGRPIIANADAVMLKELRRIGGLLKHIHNESGGVYSKETAGALVALKDYFRKLNDDR"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            8208..10880
                     /gene="traI"
                     /locus_tag="HV270_RS27040"
                     /old_locus_tag="HV270_27050"
     CDS             8208..10880
                     /gene="traI"
                     /locus_tag="HV270_RS27040"
                     /old_locus_tag="HV270_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="WP_047353284.1"
                     /translation="MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARG
                     FMMDDPKSQTAEMIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGWK
                     DHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQRE
                     QNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQT
                     WEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQ
                     QVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDE
                     LMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQ
                     KSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVS
                     FVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNP
                     ELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFIL
                     DKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIR
                     DGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRA
                     PGYENRKPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSK
                     AKPALELAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEG
                     AIRQCAPATRQKDEGRDWNDYAQRTARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQ
                     QEQAKAQKIERDNSPGMSL"
                     /name="traI"
     gene            10947..11171
                     /locus_tag="HV270_RS27045"
                     /old_locus_tag="HV270_27055"
     CDS             10947..11171
                     /locus_tag="HV270_RS27045"
                     /old_locus_tag="HV270_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="WP_043149934.1"
                     /translation="MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQC
                     LLVAQADSADTAMQQFMDEALADVDGWTE"
                     /name="antitoxin MazE family protein"
     gene            11168..11494
                     /locus_tag="HV270_RS27050"
                     /old_locus_tag="HV270_27060"
     CDS             11168..11494
                     /locus_tag="HV270_RS27050"
                     /old_locus_tag="HV270_27060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303585.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_009873354.1"
                     /translation="MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLN
                     AAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSKVGPAFGRIDADALLEVERCLAVFL
                     GIAK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            11491..11817
                     /locus_tag="HV270_RS27055"
                     /old_locus_tag="HV270_27065"
     CDS             11491..11817
                     /locus_tag="HV270_RS27055"
                     /old_locus_tag="HV270_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873353.1"
                     /translation="MKTIYTIPAPDSLGAMIEVYGEPENAWYEWRIIDGGRTVRDTGTE
                     GHSAFQGRQYGQAEIALRDALMFASGLPIDGDTPTDAASLEAGYAAKAKAEAARQGAGT
                     PGSA"
                     /name="hypothetical protein"
     Typing          2..879
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8209..10877
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctttag acattatggc ggccttcacc aatgagccgc cagaacttga tttcatctgg
       61 cccggattct tggccggaac cgtgggcgca cttgttgcac ctggcgcaac tggcaagagc
      121 ttttttgctc ttgaagcggc catgtcaatc gcttgcagtg tggcaggcgg cgaccttgta
      181 ggactggccc cggcgcacac cgggcgcgtg gtctatctcg ctggcgaaga tccacagccc
      241 gcccttgtgc ggcgtgtcca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgctgaga acctgatgct tgagccgatc atgggcaagc ggttgaacgt gatggacgag
      361 gcgcacttgc gccgcgtcat cgactacagc gcaggggcgc ggctgattgt gctggacaca
      421 ttgagccgca ttcacagcct cgacgagaac agcaacggcg acatggccca ccttgtttcc
      481 gtgttggaac acatcgcggc aaccaccggg gcggctgtcc tgtacctgca ccacgtcaac
      541 aagggcagcg cccgcgacgg ccagaccgac cagcagcagg cagcgcgggg cgcgtctgcc
      601 ctgatcgaca acgccagatg gtgcggctat gtcgccaaga tgacggagca ggaagccgag
      661 cgcctgagcg accggggctt tgatcgtgcg cccatcggca acgagcggcg cggccttttt
      721 gtccgctttg gcgtgagtaa gcagaactac gacgcgaccc cgctagaccg ctggtatcag
      781 cgacacaccg gcggggtgtt gctgccggta gagctacagg agatcaagcg tgagagcggc
      841 aacaaggggg gcaagggcta tgactcagca gcgtattaaa ccggcctatg ccaaattcga
      901 tcccgctcat gtgtttgacg ggcttttcgt ccccacgaag gggaagaagc gcgggcgact
      961 gtatgtgccg ccgcgccgct tcggcgatag tgaaatcagc tttcaagggt tcgagcagct
     1021 tggagctgat gaccaatcaa tcttgcttgc agtaagcgcc cagcttggca ttgacggctt
     1081 gatgatcgag gcaaaaccac ccggcgaaat aggccagcaa ttgcgccttg ctttggactt
     1141 tgacaacgat gatggaaggc cgctgaaaac caagaaaacc agtttgcgca gcctgctgat
     1201 tgatgctgga tatgacgacc cagaaagcgg ggcggcactt gccagaatca aggaaagcct
     1261 gaaccgaatg ggtaacgcgc agatacggga aatcaatcac gctaccggct gggattaccg
     1321 ctgcaatctc ctttcgagtc ggttcaatca caagactggg gaaatctttg ttgccgtcaa
     1381 tccgcgcctg accggggcgg tttttcgaga gcaacacgtc aaggtttcgc ttttcgagcg
     1441 caacgagctg gagagtgaag ccgccaagct gctgcactgt tggctgtgct caaacatccg
     1501 gcttggccgt tcgcttggca atggcaacgg ggcgcacatc gacacgctag cgccccacgt
     1561 atggggcaag gcggcgtggg aagaagcatc aacgaaggtt cgatcagtca ggcggggtca
     1621 actacgcgat gctttagacg aaatctcaga ccgcacacgc ggactgcaag gcggtatggg
     1681 atgggccatc gaccagacca gcagcgggct tgtgctggta agtcgtccca aggaactgcc
     1741 gttccttgag ggtgagcacg acatgcgccc cagcgaatac cacgacctca tgagccagcc
     1801 gccaatgcaa gaaccagatt tgttcgaccc gcaggactgg acgccggact gagccgcacc
     1861 tgttcaagac cgccgatgat gagagccagg cctcgctcga atgccgcgtc cggaccgcct
     1921 tcgtagacga ttttcatcgc gctctgtagg cgcgccggca tcgtagacgc tgaggtggtc
     1981 aactgatctt cgccccgctc ctcggcgtct gcctcgctag cttgctgctc aagaacagcg
     2041 ccgacggtga agtagctgat tgccatcaac gcataggtcg cgtcacctgc cgaaaagcca
     2101 gcatcgcaaa ggaagcgaag ctgcgcgtcg gctttttcca tctgcggcgc ggctggccgc
     2161 gtcccggcat gaatacgcgc gccatcgcga taagcgagca acgcccgtcg aaaactgcat
     2221 gcattgccct tcaggaacga acgccagtcg tcgtcatccc ttggcgtcga atgcgtgtga
     2281 tttatcgtca gcatggcttc ggcaagtgcg tcgagcaacg cacgcttgtt cttgaaatgc
     2341 cagtagagcg ctggctgttg caccccgagg cgctcagcca gtcggcgcgt cgttagacct
     2401 tccatgccca cgtcgttaag cagttcgagc gcggttcgga tcacggcctc gcgttggagc
     2461 ttgttcattc gcgaattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag
     2521 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
     2581 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
     2641 actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt
     2701 gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc
     2761 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta
     2821 cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg
     2881 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     2941 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     3001 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     3061 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     3121 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3181 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3241 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     3301 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     3361 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     3421 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     3481 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     3541 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
     3601 ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
     3661 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
     3721 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     3781 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     3841 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     3901 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     3961 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     4021 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     4081 ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc
     4141 ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc
     4201 atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgcc ggtagctatt
     4261 gactatccgg ctgcactagc cctgcgtcag atggctctga tccaaggcaa actgccaaaa
     4321 tatctgctgg caccggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     4381 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     4441 ctgagtgatt tttctctggt gccgccctat ccctttgtgc agcttgccac gctcaaaggg
     4501 gtttgaggtc caaccgtacg aaaacgtacg gtaagaggaa aattatcgtc tgaaaaatcg
     4561 attagtagac aagaaagtcc gttaagtgcc aattttcgat taaaaagaca ccgttttgat
     4621 ggcgttttcc aatgtacatt atgtttcgat atatcagaca gttacttcac taacgtacgt
     4681 tttcgttcta ttggccttca gaccccatat ctaatcgata ggtgaaccat cctgcacaat
     4741 tctgatgatt ttcatgaatc ctgtcgtttt taacataatg ggtattaagt gtagtgtgtt
     4801 tgtctatcaa taagagattt atctaattta attcaagccc ccggcgcagg tggtaaccac
     4861 ccccggcgca ggtggtaacc acccccggcg caggtggtaa ccacccccgg cgcaggtggt
     4921 aacctgacaa gccacaaaaa cgcccgcaaa cccttgtcag tactgagttt tcgcgttttt
     4981 ccaaaattcc ggtctaagaa gttctaaaga acttctacca tgcgcggctt tcagccgccg
     5041 ctttaggcgt cgtctgaagc cttgccttcg ggcttcgccc tgcgcgggct aaagcccttg
     5101 cagacacacc cccgcccccg ccccgccgct acccccttcc ttgcaggcga aaacgcctct
     5161 actggctttc tagggcctct gccggggctg cggtgagcgt atccagcaga tcgactatcc
     5221 gcctcatggc ttctaaccac gcttcgtgca tccagaatgg ggctatttcg ccgtgctgga
     5281 gtgtgcaaag cggattgaga acccggcgcg ggttcttcca tgtctcgacg gaaagcacca
     5341 gcccgtccgc atggatggaa aagactggta gcgctttcgg ctggcccttc ttcttgcctt
     5401 gcgtttcggt tggcttgagg tcgtcccaaa cgccttgcac ggttttgttc agttcgtcga
     5461 tgaagcgttc cggcatggtt ttcaagagtt cggcagtgat tgcgttcgcg tcctcaaatt
     5521 gctttcttgc ttcggcttcc tttttccgtc gtttttgccg ctcctgttcg tattcgtcta
     5581 aggtttgcct gtgtctgcgc tgtgcttcag cttccgcttc ttcagcttgc cttcgtgctt
     5641 cctctttggc tttctgtcgg tgtgcttcac cctctgctgt gagtgctggt tttaccagtt
     5701 cgccagactt ggcctcgatg gtgatcgaaa gcggttcgcc gcgatggtct accagatcga
     5761 acggcagacc atcagcatcg aaggcggtta ggtagagatt ggccccatag accttgtgcc
     5821 ccggcctcac agcttcgtat ttttctgctg ctgcgtgcag tgcttccgct aggcgttctg
     5881 ccgtgatttc tcctttgatc ttgattctca tggcaacctc catccgtagg tgccatccga
     5941 tgcgggcttg acgtagcaca cgcctttttc gacgtaccac cccggcctat cgcacttcgc
     6001 caagctggcg aggcttcccg tttgcgcgaa gcggtaggcc aagcgcccct ctggcgtgtt
     6061 ggcccatgcc gccgccgctt tctcgtcctt ggcttcggtg tatcccgcgc cgtatccggc
     6121 ctgatagccc gaatccttgc cgctgctgtg catgtaccag ccgaacaggc cgacgcacag
     6181 gaaggccacc gcgatgcagc ccgccgccca ctgccacatc tgctttgccg atgtgttgtg
     6241 cgcgacctct tgcgctgctg ctgctaccgc ttgcgccaaa tccgccttgg ctgcttccgc
     6301 tgatgctttc acggttgcat ccgccgttac cttgaaattg accaaggtat ctttggccgc
     6361 ttgtgctatc gcttgcggga acttctcata ctggccttgg tagtgttgca aggccatgag
     6421 caccagccac agcgcatcat tgtttttcag gccgagcgca tcccgaaccc ggtaaagact
     6481 ttgccgctcc gcgtcactag gttgcctgcc gagcagcttt gcaaagctgt cgtctaggtc
     6541 actcatggac tacggcctcc aatacctttt tggcctcgtt gcgccaccgc gtaagctccg
     6601 cacggttgcc aatgggcagt tccttgagtg ctaccgcaat gctcatgcgc ttgctgtaaa
     6661 tgtcatcgct cacacggtcg gccatgtccg ggaatgtcac cgatttcccg ccgcgttcct
     6721 ctaccgccgt gcgcagcttg gagccgttgt aaagctcgaa cttcttttct tccccgaagt
     6781 gtccattgcg taccacatga acatccgcat tcggaattgc gtccatgtat tcctttagca
     6841 gttcgaggct atcccgttgg cggttaatca cccacagcgt gaccagcttg cgcttgagtt
     6901 cttcaagcgt gctattcagt gtttcaccgt aggcgctcac gcccttgtta ttccgtgctg
     6961 ccgtgttgat taccacgacg ctatccggct tgctatcgca caggttcact agctgaatcc
     7021 atccgtccgc ctcatccaga ttgataagct ccgtttccgt gctttcttta taggctttcc
     7081 atacgtccgg gttagaggtg tcagactcaa tcagcaagac gctttcgccg cgctcttgca
     7141 ggtaatcaac cgttgccatc gtaacaagcg acttgccaac gccgcccttg ctgccaccga
     7201 tcaggtaaat gcttttcgcc atgttgctta ctccttaaat gtcgtcgctg tcaggtttcg
     7261 gggtgaatgt cgccttcgat gtatccgcag gcttcttatc ggcggggcgg ggcgggggtg
     7321 tgtctcccgg tgcttgcacc ggggcttttt tcgctggctt tgctctctgc aagtagctct
     7381 tgagcgttgg ggttgcgatg ctcagacctt cgccgcgcag cgtctcggaa atctggtcaa
     7441 gcgtgtatcc acgcttttgc aaagcggcaa tctctttgga tagcacgcgc acggcctctt
     7501 gcttgctgtg ctcctgcttc ttcttttcga ctggcggcat ctcacgaagc tttgaagcga
     7561 tagcctctag ctgctctgtt gtgtatttca ttctgctaac tcctttgctc actggttgcg
     7621 gagttgacag catagcgcct tttatgcttg ttgcaaagtg cgtcccgtgc acctaaactg
     7681 ctgatatgat agtagagtgg ttgagcaata caggatgcgg attgtcatac acatgctacg
     7741 catgttcctg ccaatccccg cctgcccatt cggcgcttcg cttctcacgg tctggctcat
     7801 cctgcaaggg gcaagcccct tgaccccgga cagtgcggcg ctttcgcttc ttactgtcca
     7861 agacacaccc cgcccccgcc cccgcccccg gcgagaagga gacatgatgc catttgaaac
     7921 gcaggggccg gaaccgctgg acgccgttat caatgtgcgg ctgaccgcag cggagaaggc
     7981 cagattgaaa gaggacgccg accttgccgg gttgagcatg tccgagcttg tccgccgccg
     8041 ctacttcggc aggccgatca ttgccaacgc cgacgccgtg atgctcaagg agctgcggcg
     8101 catcggtgga ttgctgaagc acatccacaa cgagagcgga ggcgtttaca gcaaggaaac
     8161 agccggggcg cttgttgcgc tcaaggacta cttcaggaag ctaaacgatg atcgttaaga
     8221 aggttccgaa tccgaagaag tccgcgagca aggcgcagcg catcggccag cttaccagct
     8281 atgtccgttc gccggagtcg gagagtccgc aggaaaagtg tctgtatgcc ggggcgcggg
     8341 gtttcatgat ggacgacccc aagagccaga cggcagagat gattgcactc tcacaagaag
     8401 ccgtgcgcag caaggacacc atcaatcact acgtcttgag ttggcgcgaa ggcgaacagc
     8461 caagcccgga gcaagtagag gaagcggtta gcatcttcat ggatgagctt gggtggaaag
     8521 accaccaagc catctatggc cttcattccg acacggacaa tatccacctg cacatcgtca
     8581 tcaatcgggt gcatcccgaa acgctcaaga ttgttgagaa gaaccgtgga tttgatattg
     8641 aactggcaca caaagccatt gcacggattg agcacgccca aggctggcag cgtgagcaaa
     8701 acgggcgcta tcaggtgttg gagaacgggg agctaggccg agcgccatac gacccggaaa
     8761 agccacgcca gcccgaccag aagaagcggg acatggagaa ccgcaccggg gagaagtcag
     8821 cccaccgcat cgccatcgag gacggcgcag ccatcatcaa gcaggcgcag acgtgggagc
     8881 agttgcaccg cgagttagca gccaagggga tgcgctacga aaagaccggc agcggggcga
     8941 ccgtgtttgt cggggatgtt ggcgtgaagg ccagcgatgt agaccgcaac gccagccttg
     9001 ccaagatgca gaagcggcta ggtgaatacc agccagcgcc gcagcgtcag caggtagcgc
     9061 cgcgcgagcc ggagccgatc aagccagacg tgccgggatg gaaggactac atcaccgggc
     9121 gcaaggcaca ctacgccgag aagaacgcgg acaagctggc acaggacaag cggcaggagc
     9181 aggagcgcaa gcaacttgcc gagcagcaga aggcccgccg cgatgaactc atgcgcggaa
     9241 actggaaagg caagggcgaa gtgctcaacg ccatgcgcag cgtgattgcc gccgagcagg
     9301 ccgcagagaa agcggccttg aaggagaagc accagaagga gcgcgagcag caccggcagc
     9361 ggttccgacc ctatcccgac ctggagcagt ggcagcggat gcagaaaagc ccggagcttg
     9421 ccgagcagtg gcgacaccgg gccagcgagc cgcagcgcat cgagggcgac cgcagcgagc
     9481 cgccgacgcc gcgagacatt cgcgcctatc agcctgaaat cgtgggccag caggtgcatt
     9541 acagccggaa ggaagaagcg gggcggggcg ggggtgtgtc tttcgtggac aagggcaaaa
     9601 gcatcgatat tcacgactgg cgcaaccggg acagcacgct tgccgcgtta cagctatcgg
     9661 cgcagaagtg gggcagcttc accgtgatgg ggaacgacga atacaaggcc atgtgcggga
     9721 agttagcagc agagcacggc ttcaagatca caaatccaga gcttcaggag agcatccagc
     9781 aggagcggca gcggatacag caggaaaggg tgcaggcgat gaaatcggag cagctaaagc
     9841 agttcgagcg gtacgcggaa gcggtaggcg cagagcgtta ccgggtaacg tctatcaaga
     9901 tgcgcgagga cggcagcaag caaaccttca tcctcgacaa gaaggacggc atcacgcggg
     9961 gcttcacacc gcaggagatc gagcagcgca cgccggagat gcagcgccta cagcgccggg
    10021 gcgaaaacct ctactacacg ccgctatcgg acaagaagca tcacatcctc atcgacgaca
    10081 tgaaccggga gaagctggag cggcttatcc gcgacggcta ccagcccgcc gccgtgcttg
    10141 aatccagccc cggcaactat caggccatca tcaccgtgcc gaagctgggg acggcccacg
    10201 ataaggacgt gggcaaccgg ctgagcgatg ccctgaaccg tgaatacggc gacccgaagc
    10261 tatcgggagc catccacccg caccgtgcac ccggctacga gaaccgcaag cccaagcacc
    10321 agcgggagga cggcagctat cccgaggtgc gcttgctcaa ggccgagcgc cgggagtgca
    10381 tcaaggcgct ggccttgtcc agccagatcg acgccgagta tcagcggcaa gcggccttga
    10441 aggcgcagca gcccgagcgc agcaaagcca agcccgcctt ggagcttgca gcggccagcg
    10501 gcagcgcgat cgacgcctac cagcggcatt atcgcgacgt gctcaagcgg cagcgtggcg
    10561 gcgaggtgga cttgtcccgc ttggattcca tgattgccgt gcgtatgcgc gtcaccggcc
    10621 acgatcaagc cgccatcgag ggcgctatcc gccagtgcgc cccggccacc cggcagaagg
    10681 acgagggccg cgactggaac gactacgcgc agcgcaccgc ccgctatgcc tacagcgccg
    10741 caggcgaccg ccaagccgcc gagcttggga agtaccgcca gcagtgggag aagctggaag
    10801 ggcgcgagcc tgtacgccag caggagcagg ccaaggcgca gaagatcgag cgcgataact
    10861 cgccggggat gagtctttaa atacctgttg catgtaactt gcgaggtgcg tacaatgttg
    10921 catgtaactt aattggaggc ttggaaatgg caacagcaca cgttaatcag cgggttcaga
    10981 agcatcgcga tgcattacgc atggcggggc ttcgtccggt tcaaatctgg gtgccggata
    11041 cgcgccgccc cgacttcgcg gaggagtgcc gccgccaatg cctgcttgtg gctcaggccg
    11101 acagcgccga caccgccatg cagcagttca tggatgaagc cttggcagac gtggacggct
    11161 ggacggaatg atgcgcggcg accttgtgac catcgccatg cagggcgact ttggcaagcc
    11221 acggcctgcg cttgtggttc aggccaacca gttcagcgag cacggcagcg ttaccgtgct
    11281 gcctgtcacc agcacactta atgccgcgcc gttgctgcgc gtcacggttc agccgagcgc
    11341 agagaacggt ttgcagaagc catcgcaggt gatggtcgac aaggcgatga cggtgctgcg
    11401 cagcaaggta gggccagctt tcgggcgcat cgacgcggac gcactgcttg aggttgagcg
    11461 ttgcttggcc gtgttcttgg ggattgcgaa atgaaaacga tctacaccat tcccgccccg
    11521 gattccttgg gcgcgatgat cgaggtctac ggcgagccgg aaaacgcttg gtacgagtgg
    11581 cgcatcatcg acggcgggcg caccgtgcgg gacaccggca cggaaggcca cagcgccttt
    11641 cagggacgcc agtacgggca agcagagatt gccctacgtg atgcgctcat gttcgcttct
    11701 ggcctgccca tcgacggcga cacgccgacc gatgccgcca gtctggaagc gggctatgcc
    11761 gccaaggcga aggcagaagc ggcgcggcag ggtgcgggaa cacccggcag cgcctaacca
    11821 taaccgttct acacaggaga acagc
//
