LOCUS       NZ_CP056332             4074 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00807 plasmid pRHBSTW-00807_4,
            complete sequence.
ACCESSION   NZ_CP056332
VERSION     NZ_CP056332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148699
            Assembly: GCF_013745295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T3-00807
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160.322884477053x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013745295.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:17:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,196
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,990
            CDSs (with protein)               :: 4,990
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 38 of 80
            Pseudo Genes (incomplete)         :: 41 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 14 of 80
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00807"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00807_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1932..4074,1..17)
                     /gene="mobQ"
                     /locus_tag="HV282_RS25850"
                     /old_locus_tag="HV282_25855"
     CDS             join(1932..4074,1..17)
                     /gene="mobQ"
                     /locus_tag="HV282_RS25850"
                     /old_locus_tag="HV282_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(275..967)
                     /locus_tag="HV282_RS25855"
                     /old_locus_tag="HV282_25860"
     CDS             complement(275..967)
                     /locus_tag="HV282_RS25855"
                     /old_locus_tag="HV282_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1499..1753)
                     /gene="mobC"
                     /locus_tag="HV282_RS25860"
                     /old_locus_tag="HV282_25865"
     CDS             complement(1499..1753)
                     /gene="mobC"
                     /locus_tag="HV282_RS25860"
                     /old_locus_tag="HV282_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(1755..1931)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1932..2290
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.333"
                     /coverage="100.0"
                     /evalue="8.460000000000002e-179"
     Typing          complement(276..967)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.677"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1846..4073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.327"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcggttggtc acagtgaatg tgaacaatat tttgttttgt ttaacatcgt gttatgttcg
       61 tcgatgttct tcgatgttca tcgacaggga tgaatgcaag aagcacactc acataacgca
      121 agcatagttt tgtttttccg cccccaaaaa ttgggggttc ttttttcgct gcattatagc
      181 gtttttgacg caatctttca cttcaatagt accttcgcac tactaacaca aaaaaaacgc
      241 agcagggagc gttttaggtg ccttaaaatc gattttagat cgtcatcttg atctgtttgt
      301 cttccttgaa gcgaaaatgc agggctacaa ccgtgcgccc ctttttgatc gtctcaaccg
      361 ttacagccag gtcgcttttt ttgttcagtt cgtcaacaca gggttttatt agtattttat
      421 ttaaatctct gaattgtttg tatttatttt caatccctaa cattgaacga aagtcattaa
      481 gagcaataat tctctctccc gtactgcgga attgctggag gaactcgtat attcttatac
      541 tgtaactact tgataaacct gatatgtttt ttataacaac gcgcgtgaat tgccccttca
      601 gttgcgtcag ataaggcatt accgcgtcag aaaaggttat tcgagccttg gcctcacctt
      661 tagcgtactg tgcccgatac tggatccagc ggaactcctc acgcttctcg tcattcttga
      721 gaatgatcga cctgtcccat aaacgatcga tcgcttcctg taactgaacc tcggcatttt
      781 tccgccccat gtcagggaat gaatcaaaat attccgcagc ggtaatggtc attgtctttt
      841 gcaattcagc atcagcacca gatttcaatc gactgataca cagcaacaga aaacgttgtt
      901 cctgtagggt caacttgtag ctggcctcaa ccaaagcatt cgacttgtaa gcaatcaagt
      961 ccgacataca aagatcccgt tcaaatgtgg tgatgcttta atgtgcccca caaataagaa
     1021 atgtggaata ttttttttta tatcctcaca tttctgtgaa taacacctta aattcctcca
     1081 cctttacctt aaattcctcc acctaaacac cttaaattcc tccaccttta ccttaaattc
     1141 ctccacatat accactataa catattgatt ttattaatga attttgaccc taaaacaagt
     1201 attaaaaaca agatcttaaa aacaagatct aaaaacttaa cggcgagcct gtggataact
     1261 tggggcgctg aaaacaaaaa accaataaaa acaatgtgtt gcaagaaagc agaattgcaa
     1321 gttccgaata ctccccttcc aaccctgtgg aaatccagta ttcatgcggg ttcagcgcat
     1381 atcacctgcc gaaaaaccgt gcgtatgtgc tcaacctggc gacggattca cttccgcttc
     1441 ctcgcccact cgctcgctaa cgctcggtcg ctggggctgc ggcgagcgga cgatacaatc
     1501 actgtcctgg cagcgcctcc agctcgttaa tcagcggcag tatgcgcgtc acatcagcct
     1561 gtcgtgtcac ctctgccttc aggaactgga gtaaccaggc acgcaactgc acctcctccg
     1621 gctttctgac ctttgccagc aatgctgcac ccacaacaac cttctgcgct gtgtccaggc
     1681 tccggctctt cgccttcagg cgctgtaatc tcgcctcagc atcagcaatc ttctgttcga
     1741 gcgttctgct catgatgtaa ctccgtgcgc gatgaaaaat cgcattttag cgcgtcactg
     1801 gtagtttaaa aactgagctg gcataatgca cggcaaatca cgaagtgcgc acttatacaa
     1861 tctccactcc gtttcgattg tgtgcgccct gcggggctga aagaaaacta caaaaaggca
     1921 ttacggcaga aatggcgatt tatcatctca gcatgaaaat tatctccaga agcagcggct
     1981 acagcgccgt agcttccgct gcctatcgtt ccggttcact gatgcttgat gagcgtaccg
     2041 gactgactca cgactacacc cgcaaaagcg gtgtggctga agctgtgatt ctgacccccg
     2101 cgacagcccc tgcctggtgt acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg
     2161 aacgccggaa gaactcacaa cttgcgcggg aaattgaact cgctatcccc cgtgaactgc
     2221 cgcaggatgc cgcacgggaa accgttctcg cctttgtcag agaaaacttt gtcagtcagg
     2281 gcatgattgc tgatgtggcg ttccatcaca tggataaaac aaatccccat gcgcacatca
     2341 tgctgaccac gagggctgtc ggacctgctg ggttcggcgg aaaggtcagg gactggaacg
     2401 accggacaca tgccgagacg tggcgtgcat catgggctga ccatgcaaac agagcactgg
     2461 cgaacgccgg ttatcaggaa gagatagacc atcgttcata tgagcgacag gggcttgaga
     2521 aaacgcctgg tattcacctc ggaaagtctg cctgtgcgat ggaaacacgc ggtatcgaaa
     2581 cagaacgcgg cgaacagaat cgtctgatta acaggcttaa cctggaaata cagatttccc
     2641 gcactgaact caggaacagc gggctgacag agcctgcccg ccgtgtggct gaccttctga
     2701 atatcgtgct tcctgacaac gcaacttcgt acacactacg tgacatcatc gaagcgttgc
     2761 cgaaggacag taatgcggca tggaaactca ccagtaatgc catgtcgatg atggcagata
     2821 tgcaggcgac gcgcagacgc tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc
     2881 atgctgccag cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt attcccctga
     2941 tgcaatgggc ggcaccggaa taccgtagcg aacagggaaa aatccagtcg ctgagccagc
     3001 aaatggaaaa attgcgacag cattaccgga aggtggagaa acaggacatt cccgcgagtc
     3061 agacggcatt tgaaaaacag tggcagcagt ggggggcatc cggccttgca gctctcagag
     3121 tccagttgct taaacgtgaa gaggaattgc gccggaagga gcaggaggat acagaacgta
     3181 gtcgcgcgga gcgacgagcg gaacagctca ggaaaaatga caatgccgtt atggattcag
     3241 gggtactgag cgcagtcgtt acggggtatg gcgaggcccc catgcctggt aacggggaaa
     3301 tgacctgtta ccttctgctt cacaaccgga acggagaata cacgctctgg gggaacgagc
     3361 tggagaaata ccgcaccaga atctttgagc gtgttgattt aatgcgtgac aggagtggtt
     3421 atatctgtga ccgaagcgaa atcggtcaac atccaccact gcaacgtgtt tattcctctg
     3481 cgacctttga gcaacttctg acgcaggttt gccagacatg gccgcaatat acccgcaatc
     3541 tccgccagcc gaagacatgg ccggagtctt tctgtcttgg agaagaccgc cagccagcca
     3601 tgccatcact ggcagcaagg aaggtggatt ttacgcaggg acgtctgcca ccgacgctga
     3661 tgccggtaat gagttctgtc gaccgggaaa cccgccagct tcagcttttg ctggtcatgg
     3721 gcgtggatga ctctctgggc ggtgttgtcc ggcttaatgg tacgctgtat cccgcgtttg
     3781 ccgttccctc agccgataac agccagctgg tcatcagtgc actgaccgat aaggggttac
     3841 ggtatgcagg gtatggtgtt gccgtaaatc acgatgcaga cagccacata tcacctgcgc
     3901 cggaacttat ggagtttcat ctgaaaaccc gtgaggcacc gctttttgct gccgtcaaca
     3961 caccagaaaa acagccggat catctgttcc gctcgctggg tttcaatcgt acctgggacg
     4021 aatggcggcg cgaggaagat gcaagaacac acaccaccga gaggagacat gatc
//
