LOCUS       NZ_CP056328             2694 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00821 plasmid pRHBSTW-00821_8, complete
            sequence.
ACCESSION   NZ_CP056328
VERSION     NZ_CP056328.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148702
            Assembly: GCF_013732395.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00821
  ORGANISM  Citrobacter sp. RHBSTW-00821
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00821
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149.488582115015x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,573
            Genes (coding)                    :: 5,393
            CDSs (with protein)               :: 5,393
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 35 of 180
            Pseudo Genes (incomplete)         :: 139 of 180
            Pseudo Genes (internal stop)      :: 26 of 180
            Pseudo Genes (multiple problems)  :: 17 of 180
            Pseudo Genes (short protein)      :: 1 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056328.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Citrobacter sp. RHBSTW-00821"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00821"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742663"
                     /plasmid="pRHBSTW-00821_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2440..2694,1..351))
                     /locus_tag="HV285_RS28265"
                     /old_locus_tag="HV285_28270"
     CDS             complement(join(2440..2694,1..351))
                     /locus_tag="HV285_RS28265"
                     /old_locus_tag="HV285_28270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            554..1570
                     /gene="mobV"
                     /locus_tag="HV285_RS28270"
                     /old_locus_tag="HV285_28275"
     CDS             554..1570
                     /gene="mobV"
                     /locus_tag="HV285_RS28270"
                     /old_locus_tag="HV285_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_167593153.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(1593..1919)
                     /locus_tag="HV285_RS28275"
                     /old_locus_tag="HV285_28280"
     CDS             complement(1593..1919)
                     /locus_tag="HV285_RS28275"
                     /old_locus_tag="HV285_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_228288709.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          555..1567
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacctgctcc attttttcct tataaccatc ttcaaatgtt gccgctacgc cctctgaaat
       61 catgaactta tcatcttctt catgatgaac tctgttagtg ttaaccacat aacaattttg
      121 gtataagcct tctgaaaact cagcgccagg ctcctcagcc atttccttta agctttcatc
      181 atcaaagatg tattcctttg ctaggaggta gaatagctcg ttccttttat aaccagattg
      241 ctgggttgct aatgtcagta aatggtcctc ttcttgggtt aacctaacgt taatcgtcac
      301 ttctatttgg tctgtttgat tttttttgtt tagcgatttt atgaatgaca tcttactttc
      361 ctcaccagag ttaatgtact aatcaatgat agtgaatcac tacattgaaa cagaagcatt
      421 gatcacatct ttgatttaag caatgacttt atgtcatata ttttttatta agttgaattt
      481 aacgaacgat tttgcgtagc aaagtctagt gagtacacta tcatcctgat agtggcccgc
      541 cggaggcatc gcaatgcgat cccccgactg gctggatgta cgccagaacg agggggtaag
      601 catggcgaat tacgcgataa tgcgctgcaa aaagctgacg ggaatgggca gtgtggccag
      661 cgctttgcag cactgctaca gggagcgtga gacgccgaat gctgacgctg aacggacacc
      721 agaaaactac tgctctgtgt ctcagtcggc agatgaggcg atgggaaagc tgcgggagct
      781 gttgccggaa aaacgccgta aagatgcggt actggctgtt gagtacgtta tgacggcaag
      841 ccctgagtgg tggaaagaag cgacaccgcg gcagcaggcg gagtttttcg cccattctga
      901 gcagtggctg gagaagaaat acgggaagga tcgtgttgtg gctgcggtag ttcaccggga
      961 cgaggccacc ccgcatttgt cagcgttcgt ggtgccattg acgcaggatg gacggctgtc
     1021 cgcgaaagag tttattggcg ggcggtcaaa gatgcgtgac gatcagagca cctacgctga
     1081 atcagtaaaa aaattgggtc ttgagcgcgg catcgaggga agccgtgcca cgcatcagac
     1141 agttcagcac tactaccaat cgataaaccg tggcacccgt agccaggtat caatctcgcc
     1201 agatgcgctg gagccgcgcg tattgcggaa aggaattttc accaaagacg tggaagatca
     1261 ggcagccata gccaaacgtc taagtcacgc agtgaacgat ggccttgcag gaaccatcgc
     1321 catggcctct cagagcgcgc agaatgcaaa aagagtgcgc gagctacaga agactatgga
     1381 ctcgcagcag aaacgcctac agagcgttac agagcctttt aagggccttt ccagagagca
     1441 gatgaccgaa atcctgatga tggctcagcg gtttaagcag caaaaccagg aaaaggaaaa
     1501 acagcagcgt attgaacgtg aaaaacagcg acagacgcga tcgcgtggca tgggtggtat
     1561 gagtcgttag ttttgatgcg acattccaga gatcagcttc taccgccctg tataacgctg
     1621 aggccgtttt ttagtgcttt tttgtggttg ctgacagtac ctttccaacc gaactgggga
     1681 ttcaagcgat aagttttgct ccgtcctatc ttaggacctt ccagtatgat cccgaaatca
     1741 atcaggtttt ttaccgcacg actgacattt gttttttgca tagtcagcgc ttccgcaata
     1801 tccatctgcg caacttggat gtagttctcg taatccagtt ctgcgagcaa ggccatcagc
     1861 acacgcatct ggtcatggtt tagctcattg gcaatggtaa ttagcgcggc ttggttcata
     1921 gtaaagtgcc tctggaatga tgacttttgt ttaggacgaa tcactgctac aaaaccacct
     1981 aattcctccc ctgtagtttg gtctacctgc gtgattctcc gcatctaaaa atctccattc
     2041 aggaaaaaat agttatctct caagataact aagttatctc tagagataac tattgtcaaa
     2101 aactttccgc ttgtggcgcg gcttcccagc catccccttc tgtatcttat tactctccac
     2161 aatcacacat cggcaaaatc aggtgctttt atgcctgttc tgagtgttag ggttcctatg
     2221 taagtgacgt ggaaattccg cttcctcgct cattcgctca ctacgttcgg tcatgagtct
     2281 gcggcgagcg gttcttgatt ttttaaaacg atattcggag ctttatttcg tcaagggcga
     2341 gcgtagcgag ttcatttacc cttgacgatg cactgtaaaa aacacttccg gaagtgtggt
     2401 tggtgtcaca aagtgatgcc aaccacctgt agactgggtt taatcttttt taagcagttt
     2461 atagccatct ctaatgctaa atagcgtgtg cataaacaca acgtttctcc ccagtattct
     2521 ctggatctca gacgcggtta gtggtttttc gtacctaaca aagcctgata gcttctggta
     2581 gtaggtcttg tctggcacgc cattatgatc tttctttaaa acatcaccac tggcgatacc
     2641 atgtgcaaca attccttcgc cggagcgata aagaaagact aaatctcctt tttt
//
