LOCUS       NZ_CP056327             2746 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00821 plasmid pRHBSTW-00821_7, complete
            sequence.
ACCESSION   NZ_CP056327
VERSION     NZ_CP056327.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148702
            Assembly: GCF_013732395.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00821
  ORGANISM  Citrobacter sp. RHBSTW-00821
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00821
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149.488582115015x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,573
            Genes (coding)                    :: 5,393
            CDSs (with protein)               :: 5,393
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 35 of 180
            Pseudo Genes (incomplete)         :: 139 of 180
            Pseudo Genes (internal stop)      :: 26 of 180
            Pseudo Genes (multiple problems)  :: 17 of 180
            Pseudo Genes (short protein)      :: 1 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056327.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Citrobacter sp. RHBSTW-00821"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00821"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742663"
                     /plasmid="pRHBSTW-00821_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            <448..549
                     /locus_tag="HV285_RS28245"
                     /old_locus_tag="HV285_28250"
                     /pseudo=""
     CDS             <448..549
                     /locus_tag="HV285_RS28245"
                     /old_locus_tag="HV285_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(738..1112)
                     /locus_tag="HV285_RS28250"
                     /old_locus_tag="HV285_28255"
     CDS             complement(738..1112)
                     /locus_tag="HV285_RS28250"
                     /old_locus_tag="HV285_28255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            1441..1629
                     /locus_tag="HV285_RS28255"
                     /old_locus_tag="HV285_28260"
     CDS             1441..1629
                     /locus_tag="HV285_RS28255"
                     /old_locus_tag="HV285_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1677..1904)
                     /locus_tag="HV285_RS28260"
                     /old_locus_tag="HV285_28265"
     CDS             complement(1677..1904)
                     /locus_tag="HV285_RS28260"
                     /old_locus_tag="HV285_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225622915.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRACMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          2..127
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.531"
                     /coverage="93.0"
                     /evalue="9.449999999999999e-48"
     Typing          2378..2746
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.946"
                     /coverage="82.0"
                     /evalue="4.16e-169"
ORIGIN
        1 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact
       61 atcgtcttga gtccaacccg gaaagacacg acttaacgcc attggcagca gccattggta
      121 agaggattca cggagcaggg tagtagagtg cttctacaga gttcttgaag tggcggcctg
      181 actgcggcta cactggaagg acagttttgg tatctgcgct ccgtgaaagc cagttacctt
      241 cggaagaaag agttggtagc tcttgattcc ggcaaacgaa ccaccgttgg tagcggtggt
      301 ttttttgttt acaggacatg agattacgac gagagtaaaa ggatcacaag aagatccggc
      361 tgtttaccag tgatttggct tcagtattgg tttccgccta gcggggcctt atacgtaaaa
      421 attccacacc tgacttatca cacaggggag ctccgcaagt accagagagc actgaatagc
      481 aggccacgga aatgtcttgg attccggcaa ccttcagtgg tctttgctga gctaagacag
      541 gcggcttagc gttgcacttc gaacttgaat ccgcccagaa gcattcagca gaagtcgcta
      601 acggtttgct tgcaaatgct atcaataatg ctttgcctaa agcaggaata aaaaagccac
      661 ctacacggtg gctttttttc tgtccgggaa ctagctgaat tgaacctaaa atcccggcca
      721 cgaactggta gacaccttta tccctgaaga tgtttgaaga gaaaacggcc tatttcatag
      781 gtcgtaaagt cagggtttag ctctttatca tccatcccta cagcgctccc tgcctcgatc
      841 aataccttct tcatgttgct aactttaagc ccgtctttgg taaccaagat ctttcctttt
      901 ggaagcttat gaatcacgta atccttgaag gaatgtgtgt ccttatctcc accaggcaaa
      961 gaaaagccct gacctaataa cgattttaat gaattattct cttcaattaa gttcaggaca
     1021 acatcatcaa ccgtcatgcc aaagtcattc acgtactctt ttaggctctc aatagttgat
     1081 ttctttagtc ttacgtcaga cataccattc atattattat taccccaccg ctctaatttg
     1141 aagaaataat acaacaaaat acaaattata caaataaata ttgttctgaa tacaacaagc
     1201 ggtctgtagg tgcgatgata actatgccgc accgccagat tgaacataac atccgttgcg
     1261 tgcaccgcct gagaggctct cagggcgttt ctacgacctc acctgcaaaa gaacacatct
     1321 taccccctga aaaccgctca gaaacgcgct gatgatttct gcgtacctgc atggctatgc
     1381 actcaagtga atgaagtaag atttcagtct gcgatcacca taaaaccggg aaaaagccgt
     1441 atgacaaaac aggaatcagc cgcactgaac atggcaaaat ttatccgggc gcagtcgctg
     1501 ctgctgctcg aaaaactcga cgtgctcgat cttgatgacg aggcagccga ctgtgagcga
     1561 atgcacgaac aggcagaagc gctttatcag aagctgagcg cacgcttcgg tatacaggac
     1621 ggcgaataaa gagacgaaaa gaacgctcac ttagacgaaa agcgtatcca gggcaactat
     1681 gtatatccac cgtgtacata gtcatgcctg acgctttcga gcaggtcgag catgcaggca
     1741 cgacgagctt gattggtatc ggacacaata tggcgatacg cctcaaacat aacagcaccc
     1801 gcatcaccgc ccgaacggcg aagcatagcg gaaatcatcc gcacaaagag cagacgttca
     1861 tcggggcaaa ggttcataag ttctcctggt gaaaaaccaa acatgaaagc acccatctat
     1921 taacgtcagc tgtgaacgct tcattagaag aaaataggta aacccgggaa aaagatccaa
     1981 acgttaacat cacaggtaaa cagaaacgtc gggtcgatcg ggaaattctt tcccggacgg
     2041 cgcggggttg ggcaagccgc aggcgcgtca gtgcttttag cgggtgtcgg ggcgcagccc
     2101 tgaaccagtc acgtagcgct agcggagtgt atactggcta aacttcagag atgccaagga
     2161 agggacagga tatgttttgt agcaccgcca ccacggtggc gatactccaa aacgcctggt
     2221 cagggcacag ccccgacacc ccagagccga ctgtaggtca ctggacgaac gcggcggaat
     2281 gatgtcggat gacgtcattt tgcggcgttt gccctatcct gcatcgtatt aaaaaatgca
     2341 gctatggcgc gtacggtgcc aaagataagc ccggagatat tccgcttcct cgctcactga
     2401 ctcgctccgc tcggtcgttc ggctgcggcg agcggtaccg gcttactggc ggggcggaaa
     2461 tttcctggca tcttccagga agaagcttaa cagggaagca agaaggccac ggagaagccg
     2521 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     2581 tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc tccctcgtgc
     2641 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat
     2701 ctcattccac gcctgacact cagttccggg taggcagttc gctcca
//
