LOCUS       NZ_CP056325            22672 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter sp. RHBSTW-00821 plasmid pRHBSTW-00821_5, complete
            sequence.
ACCESSION   NZ_CP056325
VERSION     NZ_CP056325.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148702
            Assembly: GCF_013732395.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00821
  ORGANISM  Citrobacter sp. RHBSTW-00821
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 22672)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00821
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 149.488582115015x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,573
            Genes (coding)                    :: 5,393
            CDSs (with protein)               :: 5,393
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 35 of 180
            Pseudo Genes (incomplete)         :: 139 of 180
            Pseudo Genes (internal stop)      :: 26 of 180
            Pseudo Genes (multiple problems)  :: 17 of 180
            Pseudo Genes (short protein)      :: 1 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056325.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Citrobacter sp. RHBSTW-00821"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00821"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2742663"
                     /plasmid="pRHBSTW-00821_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(22126..22672,1..353)
                     /locus_tag="HV285_RS28120"
                     /old_locus_tag="HV285_28125"
     CDS             join(22126..22672,1..353)
                     /locus_tag="HV285_RS28120"
                     /old_locus_tag="HV285_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538612.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_060784212.1"
                     /translation="MTINNTDIGWELYRTFLGVLEEGSLSGAARSMGIAQPTVGRHVDA
                     LETALGVTLFTRSQTGLLPTEAALALQPFALTMYSAAAALKRAAESQGSGIQGTVRVTA
                     SEVIGQEVLPPILAKLQDTHPDLTVELVLTNRVQDLLQREADIAVRMTQPRQEQLIARC
                     VGVMPLGFHAHKRYLDKHGTPSSLADLAQHALIGFDEETPFVRAARKAFPIWNRENFAV
                     RTDNDMAQIALIRAGCGIGVCQMHIAARDLNLVNILPGSIPLSLETWLTMHEDLRNNPS
                     CKVTFDALLKGLQDYVTP"
                     /name="LysR family transcriptional regulator"
     gene            complement(480..2009)
                     /locus_tag="HV285_RS28125"
                     /old_locus_tag="HV285_28130"
     CDS             complement(480..2009)
                     /locus_tag="HV285_RS28125"
                     /old_locus_tag="HV285_28130"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715399.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_055396461.1"
                     /translation="MSDDTKKCPVTHMTTDFGAPVVTNRDSLTAGPRGPLLAQDVWLNE
                     KLAGFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFIRDPRQFPDLNKAVKRDPRTNLR
                     SATNNWDYWTLLPEALHQVTVVMSDRGIPASYRHMHGFSSHTYSLWNQAGERFWVKMHF
                     RTQQGIKNLTDAEAGELVAQDRESHQRDLYEAIERGEYPKWTMFIQVMPEADAEKYALH
                     PFDLTKVWYKGDYPLIEVGEFELNKNSENFFADVEQVAFSPSNLVPGIGVSPDRMLQAR
                     LFNYADAQRYRLGVNYHQIPVNQARCPVHSNHRDGQGRVDGNYGALPHYEPNSFGQWQG
                     QPQFSEPPLKLTGNAAHWSYDKDDHNYFEQPGKLFRLMNDGQKEALFGNTGRAMGDAPE
                     FIKFRHIRNCHAADPAYGAGVAKALGINLEKALASKKDDPMYGNPLVALPA"
                     /name="catalase"
     gene            2113..>2237
                     /locus_tag="HV285_RS28785"
                     /pseudo=""
     CDS             2113..>2237
                     /locus_tag="HV285_RS28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112884.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /name="IS481 family transposase"
     gene            <2398..2484
                     /locus_tag="HV285_RS28130"
                     /old_locus_tag="HV285_28135"
                     /pseudo=""
     CDS             <2398..2484
                     /locus_tag="HV285_RS28130"
                     /old_locus_tag="HV285_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            2532..2894
                     /gene="arsD"
                     /locus_tag="HV285_RS28135"
                     /old_locus_tag="HV285_28140"
     ARG             2533..2891
                     /name="arsD"
                     /gene="arsD"
                     /gene_name="arsenite efflux transporter metallochaperone
                     ArsD"
                     /tool="amrfinderplus"
                     /drug_class="ARSENIC"
                     /identity="98.33"
                     /coverage="100.0"
     gene            2912..4663
                     /gene="arsA"
                     /locus_tag="HV285_RS28140"
                     /old_locus_tag="HV285_28145"
     ARG             2913..4660
                     /name="arsA"
                     /gene="arsA"
                     /gene_name="arsenite efflux transporter ATPase subunit
                     ArsA"
                     /tool="amrfinderplus"
                     /drug_class="ARSENIC"
                     /identity="99.14"
                     /coverage="100.0"
     gene            4712..6001
                     /gene="arsB"
                     /locus_tag="HV285_RS28145"
                     /old_locus_tag="HV285_28150"
     ARG             4713..5998
                     /name="arsB"
                     /gene="arsB"
                     /gene_name="arsenite efflux transporter membrane subunit
                     ArsB"
                     /tool="amrfinderplus"
                     /drug_class="ARSENIC"
                     /identity="98.37"
                     /coverage="100.0"
     gene            6014..6439
                     /gene="arsC"
                     /locus_tag="HV285_RS28150"
                     /old_locus_tag="HV285_28155"
     ARG             6015..6436
                     /name="arsC"
                     /gene="arsC"
                     /gene_name="glutaredoxin-dependent arsenate reductase"
                     /tool="amrfinderplus"
                     /drug_class="ARSENIC"
                     /identity="99.29"
                     /coverage="100.0"
     gene            complement(6470..>6763)
                     /locus_tag="HV285_RS28155"
                     /old_locus_tag="HV285_28160"
                     /pseudo=""
     CDS             complement(6470..>6763)
                     /locus_tag="HV285_RS28155"
                     /old_locus_tag="HV285_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /name="ArsA-related P-loop ATPase"
     gene            complement(6883..7455)
                     /locus_tag="HV285_RS28160"
                     /old_locus_tag="HV285_28165"
     CDS             complement(6883..7455)
                     /locus_tag="HV285_RS28160"
                     /old_locus_tag="HV285_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556711.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001556711.1"
                     /translation="MRLFGYARVSTSQQSLDIQIKGLKEAGVKASRIFTDKASGSSTDR
                     KGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGEMG
                     KMVVTILSAVAQAERRRILERTNEGRQEARLKGIRFGRKRIIDRNSVLALHQQGTGATD
                     IARRLSIARSTVYKILEDESRVNLSKI"
                     /name="recombinase family protein"
     gene            7619..10627
                     /locus_tag="HV285_RS28165"
                     /old_locus_tag="HV285_28170"
     CDS             7619..10627
                     /locus_tag="HV285_RS28165"
                     /old_locus_tag="HV285_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_032651769.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRRG
                     RHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTLRE
                     HTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLLPGA
                     TTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKGPVTV
                     SGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQRKLAV
                     LTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALILARACSL
                     LLDEQADDAELRETIFNSIPKSRLAESVCKVNELARPQNNNFHDEMVEQYGRVKRFLPA
                     VLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVYDAEGRIQR
                     AGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCRALGHPTDGH
                     KGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEPTSLHRLNSRV
                     RLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLMAEACNIGLEPL
                     IKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWGGGEVASADGMRF
                     VTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRDSIFVLEGLLEQQT
                     GLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVDKSANYGALDELARG
                     CADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGLAQAIMEVGRVNKTLY
                     LLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRYREGQEDQLGALGLVTN
                     AVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINMLGHYTFTLPEDILKGEL
                     RELNFNLNNELTS"
                     /name="Tn3 family transposase"
     gene            11433..11624
                     /locus_tag="HV285_RS28170"
                     /old_locus_tag="HV285_28175"
     CDS             11433..11624
                     /locus_tag="HV285_RS28170"
                     /old_locus_tag="HV285_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_191212355.1"
                     /translation="MSKQQKTVLNMAKFIQAQSLLLLEKLNEQDLDAEADLCEKLHDDA
                     EQLYRTLAIRLSNLQGDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11624..12052
                     /locus_tag="HV285_RS28175"
                     /old_locus_tag="HV285_28180"
     CDS             11624..12052
                     /locus_tag="HV285_RS28175"
                     /old_locus_tag="HV285_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_191212354.1"
                     /translation="MAHVSISEAARLASVSRPTIYKLLKSGELSYTSSVKYGKPVKIID
                     TAELMRVFGALNVVSDSKRDAVKFDDDSTAINSDFLQDLQHQVALLQTENAGLKDAVNA
                     RDEHISSLRLAMQLLEHKQPLSDTPNLTASPWWKFWQK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(12599..12898)
                     /locus_tag="HV285_RS28180"
                     /old_locus_tag="HV285_28185"
     CDS             complement(12599..12898)
                     /locus_tag="HV285_RS28180"
                     /old_locus_tag="HV285_28185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="WP_191212353.1"
                     /translation="MTITCRFSVGIESPADTDTAWGIYVPAFDGTEYGCVSAADTEEGT
                     EAAAREAILAMTTYILATGGDLRALRDAGTAEYRDHADYRYCDRWLVIDTELPE"
                     /name="antitoxin HicB"
     gene            complement(13382..13597)
                     /locus_tag="HV285_RS28185"
                     /old_locus_tag="HV285_28190"
     CDS             complement(13382..13597)
                     /locus_tag="HV285_RS28185"
                     /old_locus_tag="HV285_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            14183..15079
                     /locus_tag="HV285_RS28190"
                     /old_locus_tag="HV285_28195"
     CDS             14183..15079
                     /locus_tag="HV285_RS28190"
                     /old_locus_tag="HV285_28195"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="WP_058845346.1"
                     /translation="MKEETISLRESLSKIVMEHHEKPVISAYEINWYLYELYRDKKFNG
                     LKIGKITADEPDYRVVGDATFHLLRKGIISKCVDGVVYYINNKDKPSAQQVVCSINPCS
                     YISYINAMEWHGITDRIPSEVHVTMSTPRYLKEYFSLKIKESFPLIKNKTFLIPQLVSS
                     IPIYDGKKIIFHKKTDFKIKNEQFASGGIRVTNIGETFLDMLKFPEYCGGFNHVVSVFE
                     GFAEKYLPIIVKEFSKNGNSMDKARAGYILEDICGLTHKGIDSWADSVQRGGSRKLIAS
                     EPYSNVFSERWCISINM"
                     /name="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
     gene            15089..15970
                     /locus_tag="HV285_RS28195"
                     /old_locus_tag="HV285_28200"
     CDS             15089..15970
                     /locus_tag="HV285_RS28195"
                     /old_locus_tag="HV285_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_191212352.1"
                     /translation="MEYSIEDWVGEAPRDRVVFRQAVHIVLSAIASSDYLRPKMIMKGG
                     MLLGIRYRSTRFTEDIDFSTKMKLSELDEDEFRRELNEALLISSDSLPYGVFCAIQSLG
                     IQPKKNYENATFPSFNLKIGYARKSVSGEMERLERGQSPNTIKIDYSFNEISFDVDDLV
                     LDDDGVQSYNITDLMAEKIRSIIQQPYRNRNRRQDIYDLNYLFNCVSLDDDERFAVLTS
                     LILKSEGRIPKEDIHCNTLDREDIKEHSAKDYGTLSQEVSGDIPDFEYAYNKVNDFYKS
                     LPWDLLVNKEGE"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            15967..16635
                     /locus_tag="HV285_RS28200"
                     /old_locus_tag="HV285_28205"
     CDS             15967..16635
                     /locus_tag="HV285_RS28200"
                     /old_locus_tag="HV285_28205"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing protein"
                     /protein_id="WP_191212351.1"
                     /translation="MMSSYYHFTSQGDRNENLDRFVYLECNGFNVSFIMDGFCNNTLDM
                     HYVDYFVDVVSRGCLMQIDINHACDFVLSVVNAHDGADGKSTLSMVIYDDEVMKYISIG
                     DSRIYFLDNKERTKDDSVAQRLVDSGFSHESSIPRHPYRNRLLKFVSRKSEHQELSFKD
                     RRLNNSEKVVLCTDGFWCHCNDDVIWDMDTESKVSNAFSAVISGMLVPLDNVSVALLSI
                     "
                     /name="protein phosphatase 2C domain-containing protein"
     gene            16910..>17179
                     /gene="arsR"
                     /locus_tag="HV285_RS28205"
                     /old_locus_tag="HV285_28210"
                     /pseudo=""
     CDS             16910..>17179
                     /gene="arsR"
                     /locus_tag="HV285_RS28205"
                     /old_locus_tag="HV285_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory transcriptional
                     repressor ArsR"
                     /name="arsR"
     gene            complement(17213..20179)
                     /locus_tag="HV285_RS28210"
                     /old_locus_tag="HV285_28215"
     CDS             complement(17213..20179)
                     /locus_tag="HV285_RS28210"
                     /old_locus_tag="HV285_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="WP_001138014.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(20183..20743)
                     /locus_tag="HV285_RS28215"
                     /old_locus_tag="HV285_28220"
     CDS             complement(20183..20743)
                     /locus_tag="HV285_RS28215"
                     /old_locus_tag="HV285_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_191212473.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQSGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            complement(21046..22029)
                     /locus_tag="HV285_RS28220"
                     /old_locus_tag="HV285_28225"
     CDS             complement(21046..22029)
                     /locus_tag="HV285_RS28220"
                     /old_locus_tag="HV285_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837906.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_055396456.1"
                     /translation="MESNTALVLGATGGIGGEMARQLRDAGWTVRALRRSGEQQIERRD
                     AMNREDVLAAAEGCSVIVHAVNPPGYRRWGELVLPMLDNTIAAAHANGATIVLPGTVYN
                     YGPSSFPTLHEDSPQQPITRKGSIRVEMEQRLKQASSQNCRVLLVRAGDFFGPKLGSSW
                     FSQGMLKPGQPVKSISVPNAPGMGHQWSYLPDVARTMVELLQLRSTLDPFAAFHTAGHW
                     DADGTQLAEAICRVVVKRGGVKPKVRAFPWWLTVLAAPFVPTLRELCQMRYLWSTRVKM
                     SNAKLVSTLGHEPHTPLDEAVEAALEGMGCLQTTPVRKLSTVNSDG"
                     /name="NAD-dependent epimerase/dehydratase family protein"
ORIGIN
        1 ctcccagctc tctggctgat ctggctcagc acgcgctgat tggctttgat gaagagacgc
       61 cgttcgtgcg tgccgcacgc aaggcattcc caatctggaa ccgcgaaaac tttgcggtgc
      121 gtaccgacaa cgacatggca cagattgcgc tgatccgagc tggctgcggt attggcgtgt
      181 gccaaatgca tatagcggcg cgcgacctga atctggtgaa catattgcct ggcagcattc
      241 cgctgagcct ggagacctgg ctcaccatgc acgaagatct gcgcaataac ccgagctgca
      301 aggtgacatt tgatgcactg ttgaaggggc tacaagacta cgtgactccc tagcaagtcg
      361 acatcgatct gaccagatga ggttgcttgc caatgactga agaacctgac ttagttgacg
      421 gtgtgagaaa agagccttgg cgtctaccaa ggctcttttt catacttccg gcgcttttac
      481 tacgcaggca gggcgaccag tgggtttccg tacatagggt catccttttt tgaggccagt
      541 gccttttcaa ggttgatgcc cagggccttg gctacgcctg caccgtatgc agggtctgcg
      601 gcgtggcaat tgcggatgtg acggaacttg atgaactctg gagcgtcgcc catggctctg
      661 ccggtgttgc caaaaagcgc ttccttctga ccgtcgttca ttagacggaa caacttgcca
      721 ggttgctcga agtagttgtg gtcatctttg tcgtagctcc agtgggctgc attgccggtg
      781 agcttgagcg gcggctccga gaactgcggc tggccctgcc attggccaaa gctgttgggt
      841 tcgtagtgcg gcaaagcacc atagttgcca tcgacccgcc cctgaccatc gcggtggttg
      901 ctgtgcactg ggcagcgagc ctgattgaca gggatctggt ggtaatttac gcccagtcga
      961 tagcgctgag catcggcgta gttgaaaagg cgtgcttgca gcatacggtc cggactgaca
     1021 ccgataccag gtaccagatt gctaggggaa aaggccacct gttcaacgtc ggcgaaaaag
     1081 ttctcggagt ttttgttcag ctcgaactca ccaacttcga tgagcggata gtcgcccttg
     1141 taccagacct tggtcagatc gaacggatgc aaggcgtact tctccgcatc agcctctggc
     1201 atgacctgaa taaacatcgt ccacttggga tattcgccac gttcgatggc ttcatacaga
     1261 tcgcgctgat ggctttcacg atcctgggcg accaattcgc cggcctctgc atcggtaagg
     1321 ttcttgatgc cctgctgggt ccggaaatgc atcttgaccc agaaacgctc gccggcctgg
     1381 ttccagaggc tgtaggtgtg cgagctgaaa ccgtgcatat gacggtagct ggcagggatg
     1441 ccgcgatcgc tcatgacgac agtgacttgg tgcagagctt cgggcagaag cgtccagtaa
     1501 tcccaattgt tggtggcgct gcgcaagttt gtgcgagggt cgcgtttgac ggccttattc
     1561 agatcaggga actggcgagg atcgcggatg aagaacaccg gagtgttatt gcccaccatg
     1621 tcccagtttc cctcttcggt atagaacttc agagcaaaac cacggatatc gcgctcggca
     1681 tcggccgcac cgcgctcccc agctaccgta gtgaagcggg caaacatctc cgtcttcttt
     1741 cctacctcgc tgaaaatctt agcgcgggtg tacttggtga tgtcgtgcgt gaccgtgaat
     1801 gtgccgaagg cacccgatcc cttggcgtgc atgcggcgct ctgggatgac ctcgcgcaca
     1861 aaaccggcca gcttttcatt cagccagaca tcctgagcca ataaggggcc gcgaggacct
     1921 gcagtgaggc tgtcgcggtt agtgaccacg ggggcgccga agtcagtagt catgtgggtt
     1981 acggggcact ttttggtgtc gtcgctcatg aatttctcct aattggttgt ggggccatca
     2041 acaactatag actaggtgtt tgatcgcaag ttttgatggt gcaatatttt accttgaata
     2101 gaaggaagca ctatgggcca gattctacac ggaagtgcca caacgacaaa ggttgtccgt
     2161 cgatcaatac aaaatagtca acgctttctg cgcgccatga aatcaacctg aaaacgatag
     2221 tgaggtggaa gaaacggacc ccgatcacag gtcggcctat agggtcgcat gactgcttag
     2281 ggtcgatagc gccctcctgc atgtcatgcc tcgcaggtgc agacttgcac tgctgctagg
     2341 taaaagccgt cgcgcgcctt agcgtgctat ttttcccgtt ttctgagacg accccggtta
     2401 agtcaacagg acgacgtaca ggccatcgcc cgtaagctgg catcggccaa ctgctctggc
     2461 agcggtaagg ctgtttgtat ctaaaaattt tgcctgtaca tatatgtttt tacgaatatg
     2521 aggtgttaaa gatgaaaatg ttaacggtgt ttgatccggc gatgtgctgc agtaccggcg
     2581 tttgtggttc agatgtcgat caggttttgg tcaatttttc tgctgatgtg caatggctga
     2641 aagggcgtgg ggttcagatc gaacgttata acctggcgca gcagcctatg agcttcgttg
     2701 agaacgaaaa agcgaaagca ttccttgagg cttctggagc tgaagggctt ccgctgctgt
     2761 tgcttgatgg tgaaacggtg atggccgggc gatacccaaa acgcgctgaa ctggctcgct
     2821 ggtttggtat accgctggaa aaggtgggtt tagcttccac cagttgctgt ggtggtaaaa
     2881 cttcctgttg ttgagtatgt caggaggaca tatgaaattc ttagagaata tcccgtctta
     2941 cctgtttttc accggtaagg gaggtgtagg caaaacttcg atttcctgcg caacggctat
     3001 acgtctggct gaactgggaa agcgtgttct gcttgtcagt accgacccgg cttccaatgt
     3061 cggtcaggta ttcgatcaga ctatcggtaa cactatccag cccgtgactg ctgtgtctgg
     3121 tctttcggct ctggagattg acccgcagga cgccgcccag caataccgcg ccagaatcgt
     3181 tgaccctatt aaaggtcttt tgcctgatga cgttgttaac agcatcagtg aacagctttc
     3241 aggagcttgt acgacagaga tcgctgcatt cgacgagttt actggcttac tgactgacgc
     3301 ttctctgctg acccgctttg atcacatcat ttttgatact gccccaactg gccacactat
     3361 tcgccttctt cagcttcccg gagcctggag cagcttcatc gaaagtaacc cggatggtgc
     3421 ttcctgtctt ggtccaatgg ctgggctgga aaaacaacgc gagcaatatg ctcatgcggt
     3481 tgaggcatta tccgatcctg aacgaactcg ccttgtgctg gtcgcgcgat tgcaaaaatc
     3541 aacactgcag gaagtcgctc gtactcatga cgaattatca gctattggcc ttaaaaatca
     3601 gtatttagtc attaacggtg tcctgcctgc gagcgaagca gaacgtgatg cactggccgc
     3661 tgcaatatgg caacgggagc aagaggcgct ggcaaatctt cctgccgggt tatctgattt
     3721 gccgacagac aacttatacc tgcagccgct gaacatggtt ggtgtatccg cattgaaagg
     3781 cctgctcaac gaacacgctg agataacatc gcttcctgaa caaagcccac agaacaagcc
     3841 agagaatatg tcgttgtctg tcctggttga tgatatcgcc cgcagtgaac acggtttgat
     3901 tatgctgatg ggcaaaggtg gtgtgggcaa aaccacaatg gctgcggcta tcgccgtcag
     3961 tctggcggat aagggttttg atgtgcatct caccacctca gatcctgctg cacatctcag
     4021 tacaacgctc aatggcagcc tcaaaaatct gcaggtcagc cgaatcaacc ctcacgatga
     4081 aactgaacgc tatcgtcagc atgttcttga gacgaaaggc agggatctgg atgaggctgg
     4141 gaaacggctg ctcgaagagg atttacgttc tccttgcaca gaagagattg cggtgttcca
     4201 ggcgttttca cgcgtaattc gtgaagctgg caaacggttt gtggtcatgg atacggcacc
     4261 taccgggcat acgctattac tgcttgatgc taccggggct tatcaccgtg agattgccag
     4321 gaagatgggg gataaaggtc attttaccac tccgatgatg cagcttcagg accaggaacg
     4381 taccaaagtt ttgctggtca ctctgcctga aaccacacct gtactggaag cggcaaacct
     4441 gcagtccgat cttgaacgtg cggggattca tccctggggc tggattatca ataacagcct
     4501 ttcgattgca cagacgcaat cgccgctgct ttgccagcgc gccctacaag agcgtcctca
     4561 aatcgaggtt gtgaaaaacc agcatgcaag ccgcatagcg ttagtaccgg taatggctgc
     4621 agagcccact ggcatcgaga aactcagaga gctggttgtt taattttttt tgtatataca
     4681 gggcggcaaa gccgtcctgt ctggaggatt tatgttactg gcaggcgcta tttttgtcct
     4741 gaccatcgtt ttggttatct ggcaaccgaa gggattaggg atcggctgga gtgcgacgct
     4801 gggcgcggta ctggcgctga tctctggcgt agtccatttt ggtgatatcc ctgtagtgtg
     4861 gaatattgtc tggaatgcga cggcgacctt tattgccgtg attattatca gccttctgct
     4921 cgatgaatcg ggctttttcg aatgggcggc gctgcacgtt tcacgttggg gtaacggtcg
     4981 cgggcgtttg ctgtttacct atatcgtttt gctcggcgca gcggtggcgg cactgtttgc
     5041 caacgatggt gcggcattga ttctgacgcc aattgttatc gccatgctgc ttgcattagg
     5101 tttcagcaaa ggcactacgc tggcatttgt catggccgcc gggtttattg ccgatacggc
     5161 cagtctgccg cttatcgtgt ccaacctggt taacatcgtt tctgcagact tctttgggct
     5221 gggtttcact gagtatgcgt cggtaatggt gccggtggat atcgcagcca ttatcgccac
     5281 gctggtgatg ctgcatctgt tcttccgcaa agatattccg ccaacttacg acctggctct
     5341 cctgaaagca ccggcaaaag cgattaaaga tctggcaacc ttcagaaccg gctggatagt
     5401 gttgatcctt cttctggttg gctttttcgt ccttgagccg ttgggtatcc cggtcagtgc
     5461 gattgctgct gtaggggctg tcattctgtt tgcggtggcg aagcgaggcc atgccattaa
     5521 cactggcaaa gtgcttcgcg gtgcgccatg gcagatcgtc atattctctc tgggaatgta
     5581 tctggtggtc tatggtctgc gtaatgccgg actaacagaa tacctctccg gtgtgttgaa
     5641 cgtgctggca gataaaggac tttgggccgc gacgtttggt actggcttcc tgacggcttt
     5701 cctctcttcc atcatgaaca atatgcctac cgtgctggtt ggtgctctct ctattgatgg
     5761 cagcactgca acaggcgtta tcaaagaggc gatgattttt gccaacgtga ttggctgcga
     5821 tcttggaccg aaaattacac ctattggtag cctggcaacg ttgctctggc tgcatgtcct
     5881 ttcacagaag aacatgacca tcacatgggg atactatttc cgcaccggga tcgtcatgac
     5941 tctgcctgtg ctgtttgtaa cgctggccgc gctggcgcta cgtctctctt tcactttgta
     6001 atgagatact gatatgagca acatcaccat ttatcacaac ccagcctgcg gcacgtcgcg
     6061 taatacgctg gagatgatcc gcaacagcgg tatcgagccg accgttattc tttaccttga
     6121 gactccacct tcacgcgatg agctgctcaa actcattgcg gatatgggca tttccgtccg
     6181 ggctttgctg cgtaagaacg tcgagccgta tgaggaactg ggtcttgcag aagataaatt
     6241 tactgacgac cagctcatcg actttatgct acagcatccg attctgatca accgtccgat
     6301 cgtagtgacg ccactgggaa ctaaactttg ccgtccttca gaggttgttc tcgatatcct
     6361 tccagatgcc cagaaagcgg ctttcaccaa ggaagacggt gaaaaagtcg ttgatgattc
     6421 aggtaaaaga ctgaaataaa gacgaagggg gcatttgccc ccttcattat tacttcgcca
     6481 tttcttccag caggtcaata ccaaccggct cttcgctctg tagcgctgtt aatgcaatac
     6541 gctttgcata ctgctcttcc acatcagaga tgagaggcag ctcgcggttc gccctgatct
     6601 tcaggaaagg cgaagacggt tgagcagctg ccaggctgtt attaacgacc catgcccacg
     6661 gctcgatgcc agctcggcgt agatcctgct gcaggttagc ggcttccagt acaggtgtcg
     6721 tttcggcaag cgtcacgata atcaccttcg tcttctccgg gtctgctgct ttttcgctat
     6781 cacctgtctt cgatgtcaga aagcgagaga attcggcttc atgctgtcgc cagaacacac
     6841 tcatgcaatt gttgaatgcc tttacatcac tcatgttcgt cctcaaattt tgctcagatt
     6901 aacccggctc tcatcttcca gaattttata aacagtggaa cgggcaatac tgagccggcg
     6961 ggcaatatct gttgcaccag ttccctgctg atgaagtgcc agtacactat ttctgtcgat
     7021 gatgcgcttt cggccaaacc ggataccttt cagcctggcc tcctgccgtc cttcgttcgt
     7081 acgctccaga atcctcctgc gttcggcctg ggctacagct gaaagaatgg tcacaaccat
     7141 tttccccatt tcaccgtcgg tgctgatccc gtcgtctatg aatcggaccg cgactccctg
     7201 agcgtcgaat tcttttatca gttggatcat atcggcagtg tcgcggccaa gccggtcgag
     7261 tttcttcacc aggatgacgt caccttcctc caccttcatt cgcagcagat ccagtccttt
     7321 cctgtcggta gaactgccgg atgctttgtc ggtaaatatg cgactggctt tcacccccgc
     7381 ctctttaagc cccttgatct gaatatcgag ggactgctgg cttgttgata cccgtgcgta
     7441 accaaaaagt cgcataaaaa tgtatcctaa atcaaatatc ggacagtggg tgtctgttat
     7501 aacaaaaaat cgatttaata gacacctaga caacaccatt ttagtgtgtc cgagaattta
     7561 taatatttcg gacggttgcg aaattgttaa ttcatgacca tcaggcaggg aggccggtat
     7621 gcccgtcgat tttctgacca ctgagcagga actgaattat ggtcgctatg ttgcagaacc
     7681 caatgacgtg cagctggcgc gctattttca tcttgatgag cgggatcttg ccttcattaa
     7741 ccagcggcgg ggaaggcata accggctggg aattgcgctt cagctcacca ccgcccgttt
     7801 tctgggcacc tttctgacag acttaacccg ggttctgcca ggtgttcagc aatttgtcgc
     7861 ggtgcagctt aatattcgcc gtccagaagt ccttacccgc tacgcagaac gggttaccac
     7921 cctcagagag catactgcgc tgattaagga atattacggt taccatgaat ttggcgattt
     7981 cccctggtct ttccgcctga agcgcctgct gtacacccgc gcatggctca gtaatgagcg
     8041 acccggcctt atgtttgatt ttgccaccgc gtggctgctt cagaataagg tcctgctgcc
     8101 cggagcaacc acacttgtac gtctcgtcag tgaaatacgc gaacgggcaa atcagcggct
     8161 gtggaagaaa ctggccgcgt tgccggacag ctggcagacc gcccgcgtga cggagcttct
     8221 ggacattcct gaggggcagc gtacttcacc gctggagcaa ctgaaaaagg gaccggtcac
     8281 cgtcagcggc ccggcattta ccgaagcgct ggagcggtat atccggctgc gaaacctgga
     8341 attttcccga ctgaatttta ccggtctgcc tgctatacaa ctgcgtaatt tggcccgtta
     8401 cgcaggcatg gcgtcggtaa aatatatcgc gcgaatgcca cagcagagaa aactggccgt
     8461 actcacagca ttcgttaaag cgcaggagac tgcggcgctg gatgaggccg ttgatgtgct
     8521 cgatatgctg atactggata tcactcgtgc ggcgaagaaa acggggcaga aaaaacggct
     8581 caggacgctt aaagatcttg atcgtgctgc gctaatattg gcgcgggcat gttcattgtt
     8641 gctggatgaa caggctgacg atgctgaact gagggagacc atattcaaca gcataccgaa
     8701 aagcaggctg gcagaatccg tctgcaaggt aaatgagctg gcccggcctc agaacaacaa
     8761 tttccatgac gaaatggtgg agcagtacgg gcgggtaaag cgctttcttc cggcggtatt
     8821 gcgcgacctg catttccagg cagcgccagc cggagaacat acgttgtccg ccattcatta
     8881 tctgaccgaa ctgaacggct cgaaaaagcg catcctggac aatgcgcctg aacatattat
     8941 taccggcccc tggaaacgcc tggtatacga tgcggagggc cggatacagc gtgccggtta
     9001 ctcgctttgt ctgctggagc gccttcagga tgcactacgt cgaagggaca tctggctcga
     9061 aaacagcgat cgctggggaa atccccgcga gaagctgttg cagggggaag aatggcaggc
     9121 tcagcgggtc cccgtttgcc gggcgctggg acatcccact gatggacata aaggcgtgca
     9181 acagttggcg gtccagctag atgaaacctg gaaaaccgtc gcatcccgct ttgaaggaaa
     9241 tgcggaggtt catatttgcc atgacggtaa acatccttcc ctgactatca gcagcctgga
     9301 gaaactggag gagccaacat cgttgcatcg tctcaacagt cgggtaaggc tgctactccc
     9361 gccagtagat ttgacggaac tgttgcttga aatagatgcc agaacgggat ttacacgtga
     9421 gtttacgcat gtcagtgaat ccggggcccg ggcacaggat ctgcacatca gcctgtgcgc
     9481 ggtcctgatg gcagaggcct gcaatatcgg gctggaaccg ctgataaagc acaatatacc
     9541 ggcgctgacg cgccaccggc ttagctgggt aaaacagaat tacctccggg cagaaacgct
     9601 ggtcagcgcc aatgcccgcc tggttgattt tcagtccaca ctggagcttg ctggccgctg
     9661 gggtggtggc gaagtggctt cagctgatgg catgcgcttt gtcacgccgg tgaaaaccgt
     9721 caattcagga cctaacagaa aatattttgg ttccggacgt ggcatcacct ggtacaactt
     9781 cgtctctgat cagtactctg gattccacgg catcgttgtc cccggcacat tacgagattc
     9841 catttttgtg ctggaaggcc ttctggagca gcagacaggg ctgaatccgg ttgagatcat
     9901 gacagacaca gccggtacca gcgacattat ttttggcctc ttctggctgc tgggatacca
     9961 gttttccccc cggcttgccg atgccggtga agcggtattc tggcgagtgg ataaatcggc
    10021 aaattacggt gcactggacg aactggcacg tggttgtgcc gatctgtcga aagccgagga
    10081 tcagtgggat gagatgatgc gaaccgccgg ttcgctgaaa ctgggcacca ttcatgcttc
    10141 agaactcatt cgctctttgc tgaaaagctc gcgcccatca gggctggcac aggcgatcat
    10201 ggaagtgggg cgcgtcaaca agacgctgta ccttcttaat tatattgatg atgaggatta
    10261 tcgtcggcgg atcctgacgc agctaaaccg gggggaaggc cgccatgctg tggcgagggc
    10321 gatctgctac gggcagcgcg gtgagatcag aaagcgctat cgtgaaggtc aggaagatca
    10381 gctgggggca ctgggcctgg tcactaacgc agtggtactg tggaacacac tttatatgca
    10441 ggaagccctg agctggatac gcagtaatgg agaagaaatc ggggttgaag atatcgcccg
    10501 gttgtcccca ctgatgcacg ggcatatcaa tatgctgggg cattatacgt ttacgctacc
    10561 ggaggatatt ttgaaggggg aactgagaga actaaatttc aatttaaaca atgaattaac
    10621 ttcttagcgt acgttttcgt tccattggcc ctcaaacccc atataagcgt aaggcaataa
    10681 caacaacttg caccgatagc tcgaacagtt atatgctcta gaaacgaaaa agccacccgg
    10741 caaggtggct tttcgaaggt tcaataaacc ggcaagtttt tgaaccgtgg taactgcatc
    10801 ttagcgggaa cagtcaccaa atctgtactt tcagtgtagc cggaaacatc gcaacacttc
    10861 aagctctagt atagcctatt cagttacaac tattcccgct aatacagtta ccagtggcta
    10921 ccgccagtgg cgattcgtcg tgtcttttcg ggttggattc aagtgaaaag ttaccggaac
    10981 aggcgtggta gtcggactaa acggggagtt cgtgcatata gtccagcttg gagcgaacca
    11041 cctaaaagga acccagttgc gtgggtagga atgagaaaac gccaccacag tgtaacgaaa
    11101 cggcggactg caacgggtaa accttacggt gggaacagga gagcgcacga gggagccatc
    11161 agggggaaac tcttggtatc tttaagtcct gtcgggtttc gccacttctg atttgagtgc
    11221 ctgaaatcac ctgatttctg tgaaactcgt caggagggcg aagcctatgg aaaaacgccc
    11281 ttgcgggcgt ggccttttgc tctttattcc gcttacatca tttgttgtta gtgttcccca
    11341 tccgctcgcc gcagacaagt gaccgagcat agcgagcgaa cggtcgagga agcggaaaat
    11401 aactgtaggt aacctggagc actgacgctg tgatgtccaa acaacagaaa acggtactga
    11461 atatggctaa attcatccag gcgcaatccc tgcttttgct ggagaagctc aatgaacagg
    11521 atctcgatgc tgaggccgac ctgtgcgaaa aattgcatga tgatgcggaa cagctctacc
    11581 gtactctggc tattcgtctg agtaatctac aaggggatct gtaatggctc atgtatcaat
    11641 tagcgaagcg gcacgtctcg ccagtgtcag tcgcccaact atttacaagc tacttaaatc
    11701 tggtgaactc agttacacct catctgtaaa gtatggtaag cccgtaaaaa tcattgatac
    11761 tgctgagctt atgcgggttt tcggcgctct taatgtcgta agtgatagta aacgtgatgc
    11821 tgtaaagttt gacgacgatt ctacagcgat taacagtgat tttttacagg atttacagca
    11881 tcaggtggca cttttgcaga cggaaaacgc cgggcttaaa gatgcggtga atgcccggga
    11941 tgagcatata agttcacttc gtctggcaat gcagcttctg gagcataaac agccgttatc
    12001 tgacactccg aatcttacgg catcaccctg gtggaagttc tggcagaaat gagcgtcgta
    12061 atgcaactca gtgcaatttg tcatggtatc gacgatcttt aatgcaactt agtgcgcatt
    12121 aacgaaattt agtgcaattt agtgcaatgc cgccattctc cataaggcac gttaccgact
    12181 gtatacagtc gcgtgccaaa gggtacccct ttgaaacccg ctgctacctt tctcatgctt
    12241 aacttgtctc tttctcatac ttaattacta ccacgtggtt ttaatactcc cgttccgttc
    12301 cgggtaccat gttgtcactg gattttccct gttttctcag cccttaaccc tcagaaatgg
    12361 tgatacacaa tacgcaccct ggcggggtgc gtgactggct gaattttaat gattttcagg
    12421 agaaagctgt tttgtggaca tcatacgctc cttcatactg gaaggggaaa aatacctgta
    12481 agttactgat ttttaactca tgacgtatca tacgtcgcat accatgtcat ggctcgccgc
    12541 cccgacaccc ggccagcgcc tcacgacccc gtaataaatt tcgtgcccgt tcaccgcttc
    12601 actccggtaa ttcagtgtca atcaccagcc accggtcaca gtaccggtaa tccgcatggt
    12661 cccggtattc tgccgtgccg gcatcacgca gtgcccgaag atcgcccccg gtggccagaa
    12721 tatatgtggt catggcaagg atagcctcgc gtgctgcggc ttcggtacct tcctctgtgt
    12781 ctgctgccga cacgcagcca tactctgtac cgtcgaacgc agggacgtag atcccccatg
    12841 cggtgtctgt atctgcgggg gattcgatcc cgaccgagaa gcggcacgtg atggtcatgt
    12901 ttttctccgg tttaatgtct gatggcttta tacgaaaaga caggcggtgc cagtgcgtta
    12961 tctggcagtt tactgcatga aatgccagac agacggaaga tgccgtctgt aacgcggtgc
    13021 cagtcagtcc ggtgcataac gattcatgcc tgtaaaattc atcagcgcga ttctgaggcg
    13081 ttttcagggg gtaagacgtg tggttttgcg tctccggcgg caaaatcgcg cacaggccgt
    13141 ttcagcggtg cgcgaaatgg gacgttatgg taaataagtg ggcgtaaaaa agcccgggtt
    13201 acgggcttct ggacgctatg acttaccacg acattctctg caccaacttt gattcgttgc
    13261 accagaagga atggttctga atccgaatag agtttgcaac tcacctgcag tatgagcggt
    13321 ggttcgcttg cagttagggc atgtatgagg aagattcacc gtgcctacca tttttctatc
    13381 cttaaacgtc tccgcgttct ctgagaattt tcattgccat gcttttttct gtgttactgg
    13441 ctgagccgaa aaaaccatca gatttaatga ggcgctttag ttcttcactg cttttgcttt
    13501 ctaagcgcat tttggttgcc tggagttcat gagctttttc atccaaagcg ttagctgcgc
    13561 cttttgcaat tttaccggcc gtttcccaaa atcccattta accactcctt aatgcttatc
    13621 caattggact tgttgtgatc cgacgactcc gacatttagg gcaataatca ggcgtgccat
    13681 aaccagtacg actggtccac tcatgcttac aatccaaaca cttagcccgg tagattgtcg
    13741 gtgccattga atactccatt gattttaaaa atatttgtat agtgcactaa aattatcggc
    13801 agtttttctc ggagcttgag aggtagataa ccctaagctg atatgttgcc tttatgatgg
    13861 ttttcgtgtg tcaaggttag ctaggtaagg gatttactgt gggaagtgct ggttcgcgaa
    13921 gtgaggcgtt atggtaaatc aggacgatag gttaagttag atttaaggat gccccgaaaa
    13981 taatgtcatt aaaaatacat gaaaccttac taaactaacg tccttaggac gttagtttag
    14041 taaggaaaca taaaaaagtc aatttttgaa aaaaaacctt tatattcata tcattagaca
    14101 aatataaatc gcgatgttaa atttgacttt tgttagtaga tgatgtataa gtccttagga
    14161 cttatacatc atctggtaca tcatgaaaga agaaacgatc tccctaagag aatccttaag
    14221 taagattgtc atggaacacc atgagaaacc tgtgatcagt gcatatgaaa ttaactggta
    14281 tttatacgag ctttatagag acaaaaaatt taatggatta aagattggga aaataacggc
    14341 agacgagcct gattataggg tcgttggcga tgctactttt cacttgctta ggaaggggat
    14401 tatatctaag tgtgttgatg gggttgttta ttatataaac aacaaagata aaccttcagc
    14461 tcagcaagta gtttgttcta ttaatccatg ttcatatata tcgtatataa atgcaatgga
    14521 gtggcatgga atcactgaca gaataccaag tgaagttcat gttactatgt cgacacctcg
    14581 atacttaaaa gagtattttt ctttaaaaat aaaagaaagc ttcccattga ttaaaaataa
    14641 aaccttttta atccctcagt tagtaagttc aatacctata tatgatggga aaaagataat
    14701 ttttcataag aaaacagact tcaaaatcaa gaatgaacaa ttcgcttctg gtggcattag
    14761 agttacaaat ataggtgaga cattcttaga tatgcttaag ttcccagaat actgtggtgg
    14821 ttttaatcat gttgttagtg tttttgaagg ttttgctgag aaatatttgc ctataatagt
    14881 aaaggaattc tctaagaatg gtaactctat ggataaagct agagctgggt atatattaga
    14941 agatatttgt ggtttaactc ataaaggcat tgattcttgg gctgatagtg tccagcgagg
    15001 tggaagtcgc aagttaattg catcagagcc atattcaaat gttttttctg aaagatggtg
    15061 tatatctata aatatgtaag agggttttat ggagtattct attgaagatt gggtcggtga
    15121 ggcgccgagg gatagggttg tgtttaggca agcggtacat atagtacttt ccgctattgc
    15181 aagcagtgat taccttaggc caaaaatgat aatgaaagga gggatgcttc tgggtattag
    15241 atataggagc acaagattca cggaggatat tgatttctct acaaaaatga agctttctga
    15301 gttggatgag gatgaattca gacgtgaatt aaatgaggca cttttaatat cttctgatag
    15361 ccttccatat ggtgtttttt gtgcgatcca aagcctcggg attcagccta aaaaaaatta
    15421 tgaaaatgca acattcccat catttaattt gaagataggc tatgccagaa aaagtgtttc
    15481 cggtgagatg gagcgactgg aaagagggca aagtccaaat actataaaga tagattatag
    15541 ttttaatgaa attagctttg atgtggatga tttggttctc gatgatgatg gtgtgcaatc
    15601 gtataacata acagatctaa tggctgaaaa aatcaggtct ataattcaac agccgtatag
    15661 aaacaggaat cgccgccaag atatttatga tttaaattat ttatttaatt gcgttagtct
    15721 tgatgatgat gagcgctttg ctgttctcac ttctttaatt ttaaaatccg agggtaggat
    15781 tccgaaggaa gatattcact gtaatacatt agatcgtgaa gatataaaag aacactcagc
    15841 caaagattat ggaactttat ctcaagaggt gtccggagat attcctgatt tcgagtatgc
    15901 ttacaataag gttaatgatt tctataaatc acttccttgg gatttgctag taaataaaga
    15961 aggtgaatga tgtcatcata ttatcatttc acctctcaag gtgataggaa tgaaaattta
    16021 gatagatttg tttatctcga atgtaatggt tttaacgttt catttattat ggatggtttt
    16081 tgcaataaca ccctagatat gcactatgtc gattactttg ttgatgtggt ttctagaggt
    16141 tgtctgatgc agatagatat aaatcacgct tgtgattttg ttcttagcgt tgttaacgcc
    16201 catgatggag cggatggtaa atctacgctc tcgatggtta tatatgatga tgaagtaatg
    16261 aaatatatat ctataggtga ttcgagaatt tattttttag ataataagga aaggaccaaa
    16321 gacgactccg ttgctcaacg tttggtcgat tctggttttt ctcatgaatc cagcatacca
    16381 aggcaccctt acagaaatcg acttcttaaa tttgtatcga gaaaatcaga gcatcaagaa
    16441 ttatcattta aagatcggcg attaaataat tctgagaaag ttgttttgtg taccgatggt
    16501 ttttggtgcc attgtaatga tgatgtcata tgggatatgg ataccgagag caaagtaagt
    16561 aatgcattct cagctgtcat tagtggtatg ttggtgcctc tagataatgt atctgtagca
    16621 ttgctctcga tataggggtc tgagggccaa tggaacgaaa acgtacgcta aggaaataac
    16681 caattgttat aattgatatt attcattctt ctctatgaac tgaaagcccg cactaagcaa
    16741 tgttttagcc tcaaatgact tttttaccta cccaactaac gtaacgatcg cacatcaggt
    16801 cgtttttcaa ccgcatagtg acacctgcac tcacatgcga gaaaacacat atgattttga
    16861 cttcttgttt tggtggagag acaataccca ttcatagcag gagtacataa tgccagaaat
    16921 tgcatcccta caacttttca aaatactatc tgatgaaacc cgcctgggta tcgtgctact
    16981 gctcagggag atgggggagc tgtgcgtatg cgatctctgc acagcgctgg aacaatcaca
    17041 acccaagacc tcccgccatc tggcgatgct tcgggaaagt ggcctgttgc tggatcgcaa
    17101 gcaggggaaa tgggttcatt atcgcttatc cccgcatatt ccttcctggg ctgccctggt
    17161 gattgagcag gcctggttag gggagcccgc agaattcgga aaaaatcgta cgctaaggtt
    17221 ttccgggcat ccgtaagggc cgaaacttcc cgtcttccag tctgcggctc tgccgccaga
    17281 cgtaatcgcc ggttaggttg atgtgctccc agcccagcgg cgacaggaat tgcagcagct
    17341 cgccgtccac cggcttgccg gcctcgacca acccctgggt ggcgcgttcc aggtacaccg
    17401 tgttccacag cacgatagcc gccgtcacca ggttgaggcc gctggcccgg tagcgctgct
    17461 gctcgaagct ccgatccctg atttccccaa ggcggttgaa gaacaccgcc ctggccagcg
    17521 agttgcgcgc ctcacctttg ttcaggccgg catgcacgcg gcggcgcagt tcaacacttt
    17581 gcagccagtc caggatgaac agcgtgcgct cgatccggcc cagctcgcgc agggccacgg
    17641 ccagtccgtt ctggcgcggg tagctgccga gcttgcgcag catcagcgag gcggtgacgg
    17701 tgccctgctt gatcgagctg gccaggcgca ggatgtcgtc ccagtgggca cgcacgtgct
    17761 tgatgttcag ggtgccgccg atcagcgggc gcaacgtcgg gtaggcttgc acgccctgcg
    17821 gcacgtacag cttggtttcg ccgaggtcgc ggatgcgcgg cgcgaagcgg aagcctagca
    17881 ggtgcatcag ggcaaagacg tgatcggtga agccggccgt gtcggtgtag tgctcctcga
    17941 tccgcaggtc ggactcgtgg tacagcaggc cgtcgagcac ataggtggaa tcgcggacgc
    18001 cgacattcac cacgcgggtg ctgaacggcg cgtactggtc ggagatatgg gtatagaaca
    18061 gccgtcccgg ctcgctaccg tacttcgggt tgacgtgccc ggtgctctcg ccccggccac
    18121 ccgcgcggaa gcgctggcca tcggaggatg aggtcgtgcc gtcgccccag tgggcggcaa
    18181 aggcgtggcg atactggtgg ttgaccagct cggccaaggc cgccgaatag gtttcgtcgc
    18241 ggatgtgcca ggcttgcagc caggacagct tggcgtaggt caggccgggg ctcgactcgg
    18301 ccatcttggt cagcccgagg ttgatcgcat caccgaggat tgcggacagc agcaacgtcc
    18361 tgtctttggc ctcggccccg tccttcaagt gggtgaagtg gcggctgaag cccgtccagt
    18421 cgtccacgtc catcagcagt tcggtgatct tgatgcgcgg cagtaactgg ctggtttggt
    18481 cgatcagcgc ctgcgcccga tccggcaccg ccgcatccag cggggtgatt ttcagccctg
    18541 actcggtgag gatggcatcg ggcagctcgt tgtccttggc caggcgggtg acggtggcca
    18601 actgctcgtc cagcagctgc aaacgctctt ccaggtactg gtcgctgttc gggttgatcg
    18661 ccaggggcag ggcctgctcg cgcttgagtg cggcgaactt ctcggccggc agcaggtagt
    18721 cgtcgaagtc gcggaactgc cgcgagccct tgacccagat gtcgccggag cgcagggcgt
    18781 tcttcagctc ggacagggcg cagatttcgt agaatttccg gtcgaggcct tccggggtga
    18841 tcaccagcgg cttccagcgc ggcttgatga aggccgtggg tgcatcggcc ggcaccttgc
    18901 gcaggttgtc ggcgttcatc tcacgcaggg tctgcacggc tgccagcacg ccttgcgcgg
    18961 ccggcgcggc gcgcagttcc agcacctcca gcaaggccgg cgtgtaacgg cgcagggtgg
    19021 cgaagttctc gccgaccagg tgcaggtggt cgaagccttc cggccgggcc agcagctcgg
    19081 cctcgctgac gctctcggtg aactcgtccc agggaatcac cgcctcgatg gcggcatagg
    19141 ggtcgctgcc gctttccttc gcttccagca gcgcctggcc gatcctggag tacaggcgca
    19201 ccttgtcgtt gatcgccttg ccctgcttct ggaactgctg ctgatgcttg tgcttcgcgc
    19261 cgctgaacag cttgaccagg atgcggtcat gcagatcgac cagctcatcg atcacggtcg
    19321 cggtgctctc cagcaccacg gcggccaggg tcgcgtagcg gcgctgcggc tcgaacttac
    19381 cgaggtcttt gggcgtcatc tgcccaccct cgcgggccag cttgagcagg cggttctggt
    19441 ggatgtgccg gcccaggcct tcgggcaagt ccaccaactg aaatgtcttc agccgctcga
    19501 tgtgttccag catgtgccga gagttgggtt tcagcggtgc ctggcgcagc caggtcaacc
    19561 aggtgatgct gctgccggcc ttgagcttca acagctcgtc cagcttggcc cgatgcgagt
    19621 ccgtgagtgg ttcgaccagg gcgcggtaga cccgccgatt ggctcgcgca atggcttccg
    19681 agcaggcccg gtcaatcacg ctcagcgccg gcaggatgcg tcgtttctgc cgtaggctct
    19741 ccagggcctg accggccagc agcaagcctt tgtcggtctg ctgggccagc tcggttagct
    19801 cgcgcaccag ggcgcggaag tcggacaggc cgaacggggc cagttgcagg taggtgcgca
    19861 gttcctgggc atgctcgcga cgggtcacgt cgcgctcgcc gtactttgcc cagctcgccg
    19921 gctcggcctg gacttgcttc gccacccaca ggatgaccgg ctcgggcagc tcgctgtcgg
    19981 ttcccagcgc gtaaccgggg tagcgcagca ggcagagctg cacggcgaag ccgaggcggt
    20041 tggcgtcgcc gcgtcgctgg cggatcagcg acaggtcgga gtcgttgaag gtgtagtagc
    20101 ggatcaggtc atcctggctt tccggcagcg caagcagggt gtcccgctcc gtggccgaga
    20161 ggatcaagcg acgcggcatg tgtcagtcgt ccgtgcggag gtactggtag agggtttccc
    20221 ggctgatgtt gaactcgcgg gcaagctgcg ccttgggctc gccggccgtc gctcgctgcc
    20281 gcagggtagc agcctgctca tcggacaggg ctttcttgcg gccccggtac gcgccactct
    20341 gcttggccaa ggcgatgccc tcacgctgcc gctcgcggat cagggcgcgc tcgaactcag
    20401 cgaaggcccc catcaccgac agcatcaggt tggccatcgg cgagtcctcg ccagtgaaca
    20461 ccaggccctc cttcaggaac tcgatgcgca cgccgcgctg agtcagcttc tgtaccaagc
    20521 gacgcaggtc atcgaggttg cgggccagcc gatccatgct gtgcaccacc actgtatcgc
    20581 cttcgcggac gaagctcagc agcgcttcga gctgggggcg ctgggtgtcc ttgcccgatg
    20641 ccttgtcggt gaacaccttg ctcacctggg tttgttccag ctggcgttcc gggttctggt
    20701 caaagctgct gacccggacg tagccgatgc ggtgcccctg cacgatgtct ccttggttga
    20761 aggcggctta agtgcacttt ctgttccgtt gtgcctcaaa gcccatttct gtcaggctga
    20821 aatctataac cttcgcgggc atgtgtcaaa aaatgggaaa gcagactcta ttctgacgaa
    20881 gcggcgcggc cctgcctgac atcaagttag ggtatagatc aggttgacgc cccaactctg
    20941 ggtttcggct cccggccaga agctgtcgtc cggtaggtag ctgtgaaagg caggtctagg
    21001 ttgatcacag tcaatcaagc actgtgaaat ttgcgacaga tgtcgttagc catcactgtt
    21061 gacggtgctt aacttgcgca caggcgtcgt ctgcaagcac cccatacctt ccaaggcggc
    21121 ctccaccgcc tcatccagcg gcgtatgcgg ctcatgccca agcgtcgaga ccagcttggc
    21181 gttgctcatc tttacccgcg tgctccacaa atagcgcatc tgacatagct ctcgcagggt
    21241 cggtacaaag ggcgcagcaa gcacggtaag ccaccagggg aaggctctca ctttgggctt
    21301 gactcctccg cgcttgacta ccacgcggca gatcgcttcc gccaactggg tgccgtctgc
    21361 gtcccaatga ccggccgtgt ggaaggcggc aaagggatcc aacgtgctac gcagctgcaa
    21421 cagctccacc atagtgcggg ctacgtcggg taggtaagac cactggtggc ccatgccggg
    21481 tgcgttgggc acactaatag acttaaccgg ctgaccgggc tttagcatgc cctgcgagaa
    21541 ccagctactg cccagcttgg gtccgaagaa gtcccccgca cgcaccagga gcactctgca
    21601 gttctggctg ctggcctgct tcagacgctg ctccatctcc acacgtatgc ttcccttccg
    21661 ggtgatgggc tgctgcggtg aatcctcatg taacgtggga aaggagctgg gaccgtagtt
    21721 gtagactgtg ccaggcagca cgatagtagc tccgttagca tgcgcggcgg cgatggtgtt
    21781 gtccagcatg ggcagtacca gctcacccca acgacgataa ccgggtgggt tgactgcgtg
    21841 cactatgacg gaacagcctt ctgctgctgc cagcacgtcc tcgcggttca tggcgtcgcg
    21901 ccgctcaatt tgctgctcac cgcttcggcg caatgcacgc actgtccagc cagcatcgcg
    21961 taattgccga gccatttcgc caccaatgcc gccggtggct cctagaacca aggccgtgtt
    22021 gctttccatt cattaccccc ggatgtgttg cgatggcaag gagtttctag cgggcactgc
    22081 tattaagcaa ttgaccaaga tgtgatagtc gccatacatt ttcgtatgac catcaacaac
    22141 accgatattg gctgggagct gtatcgcacc tttttgggcg tactggaaga gggctcgctg
    22201 tccggtgcag cgcgctccat gggaattgcc cagcccaccg tggggcggca tgtcgacgca
    22261 ctggaaacag cattgggcgt gacgctgttt acccgttcgc aaacggggct actacccacc
    22321 gaggcggcgc tagcgctgca gccgtttgcc ctgaccatgt acagcgctgc cgctgcgctg
    22381 aagcgcgcgg ccgaaagcca aggctcaggt atccaaggca cagtgcgagt tacggccagc
    22441 gaggtgatag ggcaagaggt attaccacct atcctggcga aactgcagga cacacacccg
    22501 gacctgacgg tagaactggt gctgaccaac agggtgcagg acctcctgca gcgcgaagcc
    22561 gacatcgccg tgcgcatgac acagcccaga caggagcaac taattgcccg atgcgttgga
    22621 gtgatgccac ttggctttca tgcgcacaaa cgctatctgg acaagcacgg ga
//
