LOCUS       NZ_CP056313             2927 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00832 plasmid pRHBSTW-00832_7,
            complete sequence.
ACCESSION   NZ_CP056313
VERSION     NZ_CP056313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148709
            Assembly: GCF_013731735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00832
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132.73811565286x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013731735.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:16:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,980
            CDSs (total)                      :: 5,854
            Genes (coding)                    :: 5,668
            CDSs (with protein)               :: 5,668
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 73 of 186
            Pseudo Genes (incomplete)         :: 115 of 186
            Pseudo Genes (internal stop)      :: 29 of 186
            Pseudo Genes (multiple problems)  :: 24 of 186
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00832"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00832_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2798..2927,1..176)
                     /locus_tag="HV292_RS29615"
                     /old_locus_tag="HV292_29615"
     CDS             join(2798..2927,1..176)
                     /locus_tag="HV292_RS29615"
                     /old_locus_tag="HV292_29615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058876158.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(902..1324)
                     /locus_tag="HV292_RS29620"
                     /old_locus_tag="HV292_29620"
     CDS             complement(902..1324)
                     /locus_tag="HV292_RS29620"
                     /old_locus_tag="HV292_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1324..1515)
                     /locus_tag="HV292_RS29625"
                     /old_locus_tag="HV292_29625"
     CDS             complement(1324..1515)
                     /locus_tag="HV292_RS29625"
                     /old_locus_tag="HV292_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1931..2074
                     /locus_tag="HV292_RS30215"
     CDS             1931..2074
                     /locus_tag="HV292_RS30215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_085409091.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRNV
                     EV"
                     /name="replication initiation protein"
     gene            2364..2741
                     /locus_tag="HV292_RS29630"
                     /old_locus_tag="HV292_29630"
     CDS             2364..2741
                     /locus_tag="HV292_RS29630"
                     /old_locus_tag="HV292_29630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     Typing          1674..2039
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 cgttactgaa agttgctggt tatacctttt tatccctcat tgttggcgga cttcttgcca
       61 atatcattgt tcgcggatta tctgcgacaa cattcggcat tttgctcgta aatccattag
      121 caatgatatg tagcattgcc ggactgatag tccttgctat ccaacattta ccataacatc
      181 cgttatcagc accgcctgag aggccctcag cgcgtttctg acgggaaacc ggtaaaacag
      241 gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg
      301 agtatgcgcg caagtgcata gcgcggaaat tcacaccatt cagccggggt aagtcctgtt
      361 tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga
      421 agcgttgccg gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg ggttcgtcga
      481 cggccggcaa tcacgttatg gcaagtcacc ggacgactta gcacacgatt caccggcgat
      541 tcgaatcgtg aattgaatca agagtcacca tgaacaaaaa acacccaaag tgatgaattg
      601 aatcaagagt cgattcatca taacaatatg ataataaagc gtgattcaca tatgaatcgt
      661 gggcaattcg tcagcgattc gccatagagt cgtccacgac ttggatctgc actttgtaac
      721 acatcaccgc acatttattt ttcattaaac cattgttgta aataaggaat ctgcaaaatt
      781 ttaattttgc gtggggtgtg ggtattttta gtggtgagga gcgttcagag atctggcgat
      841 ttttgggaag gttcaagaac tccccgggat cgccggagag caaaactgaa acatggcagg
      901 atcaggattt tttccagaat ttccaccacg gagaatgagc cggtgaagag gggtcgtgct
      961 tgtgctccaa aagctgcata gcctggcgaa gggaatcaat atgctcatcg cgtgctttaa
     1021 cagccccttt aagaccatcg ttttcagctt caagtaaagc gattctatgt tgtaaatcat
     1081 gtaaaccaat ggtgttaacc tctgtagaaa ttgcgtcact ttttacatca tattttacag
     1141 tatcagttac accatcaaga gaaccaaaca ccctaataag ctcggataca tcaataactt
     1201 taatatcctt cccgtgcttt acaacagagg tataacttaa cttcccgctg ttaatcatct
     1261 tgtaaatagt gggacgacta accttaacaa gccgtgcagc ttcactaata gacactctgg
     1321 ccatcagtta ccatcctgta atgaatccag tctcgaggag agtgtacgga aaagatgctc
     1381 tgcatcatcg tggagtttct cacaaaggtc ggcctcagca tcaagatcaa gctcgttaag
     1441 cttctccagc aggagcagag actgattctg gatgaattta gccatgttca gcgccgtttt
     1501 ctgttgtttc gtcataaagg gcctcagagc gtcagtgctc agtgtaaaac ctgtctcttt
     1561 tccgcttcct cgctcactca gtcgctacgc tccggtcgtt cgactgcggc gagcggcagg
     1621 agcatgcata gaaaagctcg agaaaaccga gcggtcaaag gaggaaaaaa aggaaaggcc
     1681 acggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcgcag aaacggacgc
     1741 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     1801 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcac tccgctgtta
     1861 tggccgcgtt tatctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     1921 gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
     1981 tcgtcttgag tccaatccgg aaagacacga cttatcgcca ctggcagcag ccactggtaa
     2041 cggattcgca gaacaaagaa atgttgaagt gtgaggtgaa gtacagctac actgtaataa
     2101 ctaatttggt ttctgtgctc tgcgaaagcc agttacctcg gttcaaagag ttgctgctct
     2161 aagaaccttc gaaaaaaccg ccttgctggg cggttttttc gtttccaggg caagagatta
     2221 cgacgcaaca gaaaggatct caagaggatc ctattccatc cctctccccc ccaaagggaa
     2281 accagcacca ctccacgaca ccagttcttt gaagaaaatt attgaacccc ttgattttaa
     2341 taataaaaaa aacagatata atcatgatcc acaatcaccc aacttgggta ataaaatcaa
     2401 tgatttatag gttaggtatt ttatcaatat cgctgcttac agttggttgt gtttctacca
     2461 gcaacgttct tgagttaggt agggatacat atacggtttc agcaactgct gatggtttta
     2521 gagatgcggc atcagcacgt caaagtgcct ttaaaacagg ttctgagaaa tgtaatagcc
     2581 tcggtaaaaa attcctgctc actaacgaaa acgtggctaa cacaagaatg gggatagata
     2641 ccacagtaac actctacttc cgttgtcttg acgaaagaga cagcgagtat gtacgaccaa
     2701 atctacagaa agcacctgat ctacgcatcg agagtaaata attatgaatt tccttatgat
     2761 catgatccta tggtggatat cgtcattcat cacacacatg atgaaagttg gaaatgtcag
     2821 agagtacggt cccactaaaa gccatttact gttgatggtt actacgcttc ctgctggcgt
     2881 tggtgttttt atctatccga tagtagaggt gatatgggga gatctgt
//
